Patents Represented by Attorney, Agent or Law Firm Robert L. Starnes
  • Patent number: 8058513
    Abstract: The present invention relates to isolated polypeptides having feruloyl esterase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Grant
    Filed: October 30, 2009
    Date of Patent: November 15, 2011
    Assignee: Novozymes, Inc.
    Inventors: Kimberly Brown, Doreen Bohan
  • Patent number: 8044264
    Abstract: The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Grant
    Filed: May 30, 2008
    Date of Patent: October 25, 2011
    Assignee: Novozymes, Inc.
    Inventors: Alfredo Lopez de Leon, Hanshu Ding, Kimberly Brown
  • Patent number: 8034996
    Abstract: The present invention relates to isolated polypeptides having acetylxylan esterase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Grant
    Filed: September 26, 2008
    Date of Patent: October 11, 2011
    Assignee: Novozymes A/S
    Inventors: Alfredo Lopez de Leon, Hanshu Ding
  • Patent number: 8034995
    Abstract: The present invention relates to isolated polypeptides having feruloyl esterase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Grant
    Filed: December 3, 2008
    Date of Patent: October 11, 2011
    Assignee: Novozymes A/S
    Inventors: Michelle Maranta, Kimberly Brown
  • Patent number: 8034599
    Abstract: The present invention relates to isolated polypeptides having alpha-L-arabinofuranosidase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Grant
    Filed: November 20, 2008
    Date of Patent: October 11, 2011
    Assignee: Novozymes A/S
    Inventors: Michelle Maranta, Kimberly Brown, James Langston
  • Patent number: 7960160
    Abstract: The present invention relates to an isolated polypeptide having xylanase activity from Aspergillus fumigatus with a molecular weight of approximately 50 kDa by SDS-PAGE and homologous polypeptides thereof comprising an amino acid sequence having at least 95% identity with amino adds 20 to 397 of SEQ ID NO: 6; encoded by a polynucleotide comprising a nucleotide sequence having at least 95% identity with nucleotides 107 to 1415 of SEQ ID NO: 5: or encoded by a polynucleotide that hybridizes under high stringency conditions with (i) nucleotides 107 to 1415 of SEQ ID NO: 5, (ii) the cDNA sequence of (i), or (iii) a full-length complementary strand of (i) or (ii). The present invention also relates to a method for producing such polypeptides having xylanase activity comprising: (a) cultivating a wild-type Aspergillus fumigatus cell under conditions conducive for production of the polypeptide; and (b) recovering the polypeptide.
    Type: Grant
    Filed: February 9, 2005
    Date of Patent: June 14, 2011
    Assignee: Novozymes, Inc.
    Inventors: Debbie Yaver, Paul Harris, Suzanne Otani, Janine Lin, Haiyan Ge
  • Patent number: 7960149
    Abstract: The present invention relates to isolated polypeptides having beta-glucosidase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.
    Type: Grant
    Filed: February 17, 2010
    Date of Patent: June 14, 2011
    Assignee: Novozymes, Inc.
    Inventors: Paul Harris, Elizabeth Golightly
  • Patent number: 7955830
    Abstract: The present invention relates to isolated polypeptides having lipase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.
    Type: Grant
    Filed: November 11, 2010
    Date of Patent: June 7, 2011
    Assignees: Novozymes, Inc., Novozymes A/S
    Inventors: Debbie Yaver, Suzanne Otani, Janine Lin, Christopher Amolo, Kim Borch, Shamkant Anant Patkar, Michael Lamsa, Barbara Cherry
  • Patent number: 7943350
    Abstract: The present invention relates to methods for degrading a lignocellulosic material, comprising: treating the lignocellulosic material with an effective amount of one or more cellulolytic enzymes in the presence of at least one surfactant selected from the group consisting of a secondary alcohol ethoxylate, fatty alcohol ethoxylate, nonylphenol ethoxylate, tridecyl ethoxylate, and polyoxyethylene ether, wherein the presence of the surfactant increases the degradation of lignocellulosic material compared to the absence of the surfactant.
    Type: Grant
    Filed: June 22, 2007
    Date of Patent: May 17, 2011
    Assignee: Novozymes, Inc.
    Inventors: Elena Vlasenko, Joel Cherry, Feng Xu
  • Patent number: 7932073
    Abstract: The present invention relates to variants of a parent glycoside hydrolase, comprising a substitution at one or more positions corresponding to positions 21, 94, 157, 205, 206, 247, 337, 350, 373, 383, 438, 455, 467, and 486 of amino acids 1 to 513 of SEQ ID NO: 2, and optionally further comprising a substitution at one or more positions corresponding to positions 8, 22, 41, 49, 57, 113, 193, 196, 226, 227, 246, 251, 255, 259, 301, 356, 371, 411, and 462 of amino acids 1 to 513 of SEQ ID NO: 2 a substitution at one or more positions corresponding to positions 8, 22, 41, 49, 57, 113, 193, 196, 226, 227, 246, 251, 255, 259, 301, 356, 371, 411, and 462 of amino acids 1 to 513 of SEQ ID NO: 2, wherein the variants have glycoside hydrolase activity. The present invention also relates to nucleotide sequences encoding the variant glycoside hydrolases and to nucleic acid constructs, vectors, and host cells comprising the nucleotide sequences.
    Type: Grant
    Filed: August 8, 2007
    Date of Patent: April 26, 2011
    Assignee: Novozymes, Inc.
    Inventors: Sarah Teter, Connie Ward, Joel Cherry, Aubrey Jones, Paul Harris, Jung Yi
  • Patent number: 7910804
    Abstract: The present invention relates to isolated polypeptides having alpha-glucosidase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.
    Type: Grant
    Filed: January 29, 2010
    Date of Patent: March 22, 2011
    Assignee: Novozymes, Inc.
    Inventors: Debbie Yaver, Janine Lin, Alexander Blinkovsky, Kimberly Brown, Michael Rey
  • Patent number: 7868227
    Abstract: The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Grant
    Filed: June 24, 2010
    Date of Patent: January 11, 2011
    Assignees: Novozymes, Inc., Novozymes A/S
    Inventors: Nikolaj Spodsberg, Paul Harris, Randall Kramer
  • Patent number: 7824884
    Abstract: The present invention relates to isolated polypeptides having endoglucanase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.
    Type: Grant
    Filed: April 27, 2006
    Date of Patent: November 2, 2010
    Assignee: Novozymes, Inc.
    Inventors: Paul Harris, Alfredo Lopez de Leon, Michael Rey, Hanshu Ding, Elena Vlasenko
  • Patent number: 7820408
    Abstract: The present invention relates to methods of improving the introduction of DNA into bacterial host cells.
    Type: Grant
    Filed: November 29, 2007
    Date of Patent: October 26, 2010
    Assignee: Novozymes, Inc.
    Inventors: Michael Thomas, Michael Rey
  • Patent number: 7811806
    Abstract: The present invention relates to methods for producing a hyaluronic acid, comprising: (a) cultivating a Bacillus host cell under conditions suitable for production of the hyaluronic acid, wherein the Bacillus host cell comprises a nucleic acid construct comprising a hyaluronan synthase encoding sequence operably linked to a promoter sequence foreign to the hyaluronan synthase encoding sequence; and (b) recovering the hyaluronic acid from the cultivation medium. The present invention also relates to an isolated nucleic acid sequence encoding a hyaluronan synthase operon comprising a hyaluronan synthase gene and a UDP-glucose 6-dehydrogenase gene, and optionally one or more genes selected from the group consisting of a UDP-glucose pyrophosphorylase gene, UDP-N-acetylglucosamine pyrophosphorylase gene, and glucose-6-phosphate isomerase gene.
    Type: Grant
    Filed: December 20, 2002
    Date of Patent: October 12, 2010
    Assignee: Novozymes, Inc.
    Inventors: Alan Sloma, Regine Behr, William Widner, Maria Tang, David Sternberg, Stephen Brown
  • Patent number: 7771983
    Abstract: The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Grant
    Filed: December 4, 2009
    Date of Patent: August 10, 2010
    Assignee: Novozymos, Inc.
    Inventors: Nikolaj Spodsberg, Paul Harris, Randall Kramer
  • Patent number: 7741074
    Abstract: The present invention relates to isolated polypeptides having endoglucanase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Grant
    Filed: December 3, 2008
    Date of Patent: June 22, 2010
    Assignee: Novozymes, Inc.
    Inventors: Alfredo Lopez de Leon, Michael Rey
  • Patent number: 7741466
    Abstract: The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.
    Type: Grant
    Filed: March 21, 2008
    Date of Patent: June 22, 2010
    Assignee: Novozymes, Inc.
    Inventors: Kimberly Brown, Paul Harris, Elizabeth Zaretsky, Edward Re, Elena Vlasenko, Keith McFarland, Alfredo Lopez de Leon
  • Patent number: 7705137
    Abstract: The present invention relates to microbial trypsin variants having chymotrypsin-like activity, comprising: (a) a one or more substitutions corresponding to positions 144, S193A, 198, 201, 218, 223, 227, 228, 229, 230, and 231 of amino acids 25 to 248 of SEQ ID NO: 2, (b) one or more deletions corresponding to positions 192, 197, and 226 of amino acids 25 to 248 of SEQ ID NO: 2; and (c) an insertion between positions corresponding to positions 224 and 225 of amino acids 25 to 248 of SEQ ID NO: 2. The present invention further relates to nucleotide sequences encoding microbial trypsin variants having chymotrypsin-like activity; nucleic acid constructs, expression vectors, and recombinant host cells comprising such nucleotide sequences; and methods of producing microbial trypsin variants having chymotrypsin-like activity or a precursor thereof.
    Type: Grant
    Filed: January 30, 2008
    Date of Patent: April 27, 2010
    Assignee: Novozymes, Inc.
    Inventors: Alan Klotz, Kimberly M. Brown, Elizabeth J. Zaretsky
  • Patent number: 7691574
    Abstract: The present invention relates to methods for monitoring differential expression of a plurality of genes in a first Bacillus cell relative to expression of the same genes in one or more second Bacillus cells using microarrays containing Bacillus genomic sequenced tags. The present invention also relates to computer readable media and computer-based systems. The present invention further relates to substrates containing an array of Bacillus licheniformis or Bacillus clausii GSTs.
    Type: Grant
    Filed: August 12, 2005
    Date of Patent: April 6, 2010
    Assignees: Novozymes, Inc., Novozymes A/S
    Inventors: Randy M. Berka, Ib Groth Clausen, Alexandre Bolotine, Alexei Sorokine, Alla Lapidus