Patents Represented by Attorney, Agent or Law Firm Steve T. Zelson
  • Thermostable xylanase from a strain of Rhodothermus marinus
    Patent number: 5395765
    Abstract: A xylanase obtained from Rhodothermus marinus, the method of obtaining it and a process for its use are disclosed. The xylanase has an optimum temperature of from 85.degree. to 100.degree. C., a relative activity of more than 50% in the interval of from pH 5 tp pH 8 after incubation for 5 minutes at 65.degree. C., a relative temperature stability at 80.degree. C. of more than 80% after incubation at pH 7 for 3 hours, a pH optimum of about 6 and an isoelectric point in the range of from 3 to 7. The xylanase can be obtained from the strains ATCC 43812, ATCC 43813 or a mutant thereof. The process for obtaining the xylanase involves cultivation of a xylanase-producing strain of Rhodothermus marinus in a suitable nutrient medium containing carbon and nitrogen sources and inorganic salts followed by recovery of the xylanase. Also disclosed is a process for the treatment of lignocellulosic pulp with the xylanase.
    Type: Grant
    Filed: March 22, 1994
    Date of Patent: March 7, 1995
    Assignee: Novo Nordisk A/S
    Inventors: Leif A. L. Dahlberg, Olof P. Holst, Lisbeth Anker
  • Yeast processing system
    Patent number: 5395922
    Abstract: A polypeptide for production in yeast comprises a fusion of a signal peptide, a leader peptide and a heterologous protein or polypeptide. The polypeptide is modified in its amino acid sequence adjacent to a yeast processing site positioned between the C-terminal end of the leader peptide and the N-terminal end of the heterologous protein so as to provide a presentation of the processing site which makes it accessible to proteolytic cleavage. Such a presentation is provided by adding one or more amino acids (at least one of which is negatively charged) to either the C-terminal end of the leader or the N-terminal end of the protein, or both. The heterologous protein may, for instance, be aprotinin or insulin precursor or an analogue thereof.
    Type: Grant
    Filed: February 15, 1994
    Date of Patent: March 7, 1995
    Assignee: Novo Nordisk A/S
    Inventors: Soren Bjorn, Kjeld Norris, Fanny Norris
  • Use of an enzyme containing granulate in a method for production of a pelletized fodder
    Patent number: 5391371
    Abstract: An enzyme containing T-granulate, coated with a coating agent comprising a high melting fat or wax, is combined with a mixture containing fodder components, and the combination is steam treated and subsequently pelletized. The method considerably improves the stability of the enzyme in the pelletized mixture.
    Type: Grant
    Filed: June 4, 1993
    Date of Patent: February 21, 1995
    Assignee: Novo Nordisk A/S
    Inventors: Kim T. Jacobsen, Poul E. Jensen
  • Detergent composition comprising an alkyl sulfate and a subtilisin variant
    Patent number: 5389307
    Abstract: This invention relates to a detergent composition comprising an alkyl sulphate and one or more subtilisins and/or subtilisin variants, wherein said subtilisins and subtilisin variants have a glutamic acid residue in position 195 and/or an alanine residue in position 222.
    Type: Grant
    Filed: April 6, 1993
    Date of Patent: February 14, 1995
    Assignee: Novo Nordisk A/S
    Inventors: Poul Lindegaard, Dorrit A. Aaslyng
  • Assay method using internal calibration to measure the amount of analyte in a sample
    Patent number: 5387503
    Abstract: This invention relates to a method of determining the amount of test analyte in a sample using internal calibration comprising mixing a sample with a predetermined amount of a calibrator analyte foreign to the sample and with a comparable behavior in an assay to that of the test analyte, contacting the mixture with a solid support containing, each in a separate area, a regent for binding the test and calibrator analytes, respectively, contacting the solid support with a mixture of labelled reagents for binding the test and calibrator analytes, respectively, and determining the amount of test analyte in the sample by comparing the levels of labelled reagent bound to the test and calibrator analytes.
    Type: Grant
    Filed: November 12, 1992
    Date of Patent: February 7, 1995
    Assignee: Novo Nordisk A/S
    Inventors: Johan Selmer, Fritz Poulsen
  • Insulin preparations containing nicotinic acid or nicotinamide
    Patent number: 5382574
    Abstract: Insulin preparations containing nicotinamide or nicotinic acid or a salt thereof shown interesting pharmacological properties.
    Type: Grant
    Filed: May 20, 1992
    Date of Patent: January 17, 1995
    Assignee: Novo Nordisk A/S
    Inventor: Klavs H. Jorgensen
  • Pure C3b inactivator and a process for producing said protein
    Patent number: 5378811
    Abstract: A pure Factor I protein essentially free of infectious virus. Factor B and C3. The protein is derived from plasma and pasteurized by heating to a temperature of 50.degree. to 65.degree. C. for 0.5 to 100 hours in the presence of one or more stabilizers for Factor 1. Preparations containing the protein are useful in the treatment of Factor I deficiency and autoimmune diseases.
    Type: Grant
    Filed: July 9, 1993
    Date of Patent: January 3, 1995
    Assignee: Novo Nordisk A/S
    Inventors: Karina O. Alsoe, Jesper Kihl
  • Vector and method for making tissue factor pathway inhibitor (TFPI) analogues in yeast
    Patent number: 5378614
    Abstract: A method for making TFPI analogues lacking part of the C-terminal end of the native TFPI molecule is described by cultivation of a yeast strain transformed with an expression vector containing a DNA sequence encoding such TFPI analogues. The TFPI analogues will at least contain the two first Kunitz domains and lack the third Kunitz domain.
    Type: Grant
    Filed: March 2, 1993
    Date of Patent: January 3, 1995
    Assignee: Novo Nordisk A/S
    Inventors: Jens G. L. Petersen, Ole J. Nordfang
  • Antipsychotic piperidine derivatives
    Patent number: 5378714
    Abstract: The present invention relates to therapeutically active piperidine derivatives, of formula 1 ##STR1## wherein, R.sup.1, R.sup.2, A, X and Y are as defined in the specification. The compounds are useful in the treatment of indications related to the CNS-system, cardiovascular system or to gastrointestinal disorders.
    Type: Grant
    Filed: November 19, 1992
    Date of Patent: January 3, 1995
    Assignee: Novo Nordisk A/S
    Inventors: John B. Hansen, Lone Jeppesen, Frederik C. Gronvald
  • Detergent additive granulate and detergent
    Patent number: 5376288
    Abstract: The detergent additive granulate is a coated co-granulate containing at least one enzyme and at least one photobleaching agent. If a detergent, a component of which is such detergent additive granulate, is used for laundering, the photobleaching agent will not tend to adhere selectively to the laundry during the initial washing process.
    Type: Grant
    Filed: May 28, 1993
    Date of Patent: December 27, 1994
    Assignee: Noro Nordisk A/S
    Inventors: Per Falholt, Erik K. Markussen
  • Heterocyclic compounds
    Patent number: 5376668
    Abstract: The present invention relates to therapeutically active azacyclic compounds, a method of preparing the same and to pharmaceutical compositions comprising the compounds. The novel compounds are useful as stimulants of the cognitive function of the forebrain and hippocampus of mammals and especially in the treatment of Alzheimer's disease, severe painful conditions and glaucoma.
    Type: Grant
    Filed: March 5, 1993
    Date of Patent: December 27, 1994
    Assignee: Novo Nordisk A/S
    Inventors: Per Sauerberg, Preben H. Olesen, Charles H. Mitch
  • Cell wall lytic enzymes from bacillus pabuli
    Patent number: 5374545
    Abstract: A bacteriolytic enzyme complex is obtained from a bacterial culture of Bacillus pabuli strains, e.g. , isolates 350-2 (NRRL B-18446) and 391-1 (NRRL B-18447). This bacteriolytic enzyme complex is useful as an antibacterial agent against both Gram-positive and Gram-negative bacteria. The enzyme complex may be produced by cultivating the B. pabuli microorganisms in an aqueous medium containing cornsteep liquor, after which the lyric enzyme complex can be recovered from the fermentation broth.
    Type: Grant
    Filed: July 1, 1993
    Date of Patent: December 20, 1994
    Assignee: Novo Nordisk A/S
    Inventors: Chi-Li Liu, Janet M. Overholt
  • Aprotinin analogues and a process for the production thereof
    Patent number: 5373090
    Abstract: This invention relates to novel aprotinin analogues having a selected inhibition profile against serine proteases.
    Type: Grant
    Filed: November 19, 1990
    Date of Patent: December 13, 1994
    Assignee: Novo Nordisk A/S
    Inventors: Kjeld Norris, Lars C. Petersen
  • Hyperthermostable alpha-amylase
    Patent number: 5370997
    Abstract: A hyperthermostable alpha-amylase is provided by culturing strains of Pyococcus. These enzymes are of great interest for industrial applications, for example in starch liquefaction processes. The microorganisms Pyococcus woesei or Pyococcus furiosus are capable of producing the desired alpha-amylase with the following properties:(a) a pH optimum between 4.0 and 6.0;(b) a temperature optimum between 80 and 120 degrees centigrade;(c) an activity which is essentially independent of calcium ions; and(d) a residual activity of 100% after one hour at 90 degrees centigrade and of at least 80% after one hour at 100 degrees centigrade in the presence of stabilizing amounts of substrate.
    Type: Grant
    Filed: January 29, 1993
    Date of Patent: December 6, 1994
    Assignee: Novo Nordisk A/S
    Inventors: Garabed Antranikian, Rainhard Koch, Andreas Spreinat
  • Imidazoquinazoline compounds and their use
    Patent number: 5371080
    Abstract: Imidazoquinazoline compounds having the formula ##STR1## wherein Q is ##STR2## --COOR.sup.8 or --CN; wherein R.sup.1 is H, alkyl, unsubstituted or substituted cycloalkyl, alkoxy, alkoxyalkyl or CF.sub.3 ; R.sup.8 is alkyl; R.sup.2 and R.sup.3 independently are H, alkyl optionally substituted, hydroxyalkyl, alkoxy, alkoxyalkyl, alkoxycarbonylalkyl, formylalkyl, or an acetal thereof, or R.sup.2 and R.sup.3 together with the N-atom form a 4-6 membered monocyclic amino group in which ring system one or more of the carbon atoms may be exchanged with N, O or S, sulphinyl, sulphonyl or carbonyl or an acetal thereof, each of these ring systems optionally being substituted; R.sup.4, R.sup.5, R.sup.6 and R.sup.7 independently are H, OH, halogen, CF.sub.3, NO.sub.2, NH.sub.2, CN, alkyl, alkenyl, alkynyl, alkoxy, alkoxyalkyl or alkoxycarbonyl.
    Type: Grant
    Filed: December 15, 1992
    Date of Patent: December 6, 1994
    Assignee: Novo Nordisk A/S
    Inventors: Holger C. Hansen, Marit Kristiansen
  • Method for protection of proteolysis-susceptible protein during protein production in a fluid medium
    Patent number: 5371198
    Abstract: A process for the microbial production of a protein susceptible to inactivation in a fluid production medium by continuously and reversibly protecting said protein against said inactivation during the production stage, separating the protein from the production medium, deprotecting the protein, and recovering the protein product. The process is especially useful for obtaining substantially increased yields of the protein in question by continuously precipitating said protein.
    Type: Grant
    Filed: March 9, 1992
    Date of Patent: December 6, 1994
    Assignee: Novo Nordisk A/S
    Inventors: Per L. Joergensen, Poul E. Pedersen, Joergen Petersen, Torben K. Nielsen, Jan. M. Mikkelsen
  • Enzymatic deinking process with pH shift and addition of alkaline cellulase
    Patent number: 5364501
    Abstract: Good deinking of waste paper can be obtained at high consistency by first pulping at high pH, then lowering the pH, adding alkaline cellulase and letting the cellulase act during continued pulping and/or maceration. The improved deinking results in higher whiteness and better cleanliness (fewer ink particles) in paper made from the treated pulp after separation of ink particles. This cellulase treatment results in better operation of certain equipment such as thickeners and in better drainage of the pulp during paper making.
    Type: Grant
    Filed: October 14, 1992
    Date of Patent: November 15, 1994
    Assignee: Novo Nordisk A/S
    Inventors: Jean-Luc A. G. Baret, Marc Leclerc, Jean-Pierre Lamort
  • Proteases
    Patent number: 5362414
    Abstract: This invention is in the field of detergent proteases derived from strains of a new Bacillus sp. More specifically, the invention is directed towards a protease derived from a strain of Bacillus sp. TY 145. Moreover, the invention is directed towards a process for the preparation of the protease the use of the protease enzyme, and detergent composition comprising the protease of the invention.
    Type: Grant
    Filed: August 18, 1993
    Date of Patent: November 8, 1994
    Assignee: Novo Nordisk A/S
    Inventors: Helle Outtrup, Claus Dambmann, Poul Lindegaard
  • Alkaline protease from Bacillus J 20
    Patent number: 5358865
    Abstract: An alkaline protease isolated from a strain of Bacillus sp. J 20 is disclosed. The protease has a mass of about 29 kD, a pI of about 8.8, a pH optimum above 10, and a temperature optimum of about 45.degree. to 65.degree. C. The use of the protease as a detergent enzyme, as well as detergent compositions and detergent additives comprising the protease is also disclosed.
    Type: Grant
    Filed: February 22, 1993
    Date of Patent: October 25, 1994
    Assignee: Novo Nordisk A/S
    Inventors: Helle Outtrup, Claus Dambmann, Dorrit A. Aaslyng
  • Storage case for a blood coagulation meter
    Patent number: D353711
    Type: Grant
    Filed: July 15, 1993
    Date of Patent: December 27, 1994
    Assignee: Novo Nordisk A/S
    Inventor: Steve McGugan