Abstract: Synthetic leaders for effective secretion of proteins in yeast are provided. Also provided are replicable yeast vectors containing a DNA-sequence encoding the synthetic leader positioned upstream to a DNA-sequence encoding the desired product and operably connected with promoter and signal sequences. There are also provided yeast strains transformed with such vectors and a method for producing proteins by means of the transformed yeast strains.

Abstract: The invention relates to an apparatus for simultaneous injection of two different kinds of preparations in a preset proportion.

Abstract: The present invention relates to an improved method for isolating brush border membranes from insects. The method can be used to isolate brush border membranes from small insects.

Abstract: Novel extrinsic pathway inhibitors (EPI) are provided wherein one or more of the amino acid residues of native EPI have been deleted. A preferred group of the novel EPI analogues have no or a low heparin binding capacity. The novel EPI analogues can be used for the treatment of patients having coagulation disorders or cancer.

Abstract: Novel alkaline serine proteases produced by Nocardiopsis sp. strains, for example by aerobic cultivation of strain 10R (NRRL-18262) and Nocardiopsis dassonvillei strain M58-1 (NRRL 18133), the proteases being characterized by a pH optimum of about 8, and by retention of at least 60% of maximum activity at pH 7-11 measured with casein as substrate.The proteases of the invention may be distinguished from the known protease of the Nocardiopsis strain ZIMET 43647 (of East German 2004328) and known Bacillus and fungal alkaline proteases. The Alkaline proteases of strains 10R and M58-1 may also be distinguished from each other.The protease preparations of this invention are useful detergent additives for cold water laundering.

Abstract: Novel aryloxyphenylpropylamines having the formula ##STR1## wherein X is H, cyano, halogen, halogenoalkyl, C.sub.1-6 -alkoxy, C.sub.1- -alkyl, C.sub.1-5 -alkanoyl, C.sub.3-5 -alkylene, aryloxy- or aralkoxy, andR is 3,4-methylenedioxy, aryl or heteroaryl which are optionally substituted with one or more cyano, halogeno, C.sub.1-6 -alkyl, C.sub.1-6 -alkoxy, C.sub.1-6 -alkenyl, trifluoromethyl, C.sub.3-5 -alkylene, aryloxy or aralkoxy; andR.sup.1 and R.sup.2 independently is C.sub.1-10 -alkyl, C.sub.3-7 -cycloalkyl, C.sub.2-10 -alkenyl, C.sub.3-6 -cycloalkyl-C.sub.1-5 -alkyl, optionally substituted with C.sub.1-5 -alkoxy or cyano; orR.sup.1 and R.sup.2 may together form a carbocyclic ring and a salt thereof with a pharmaceutically acceptable acid,provided however that R.sup.1 is not C.sub.3-7 -cycloalkyl, C.sub.1-10 -alkyl, or alkenyl which may be straight, branched or cyclic, unsubstituted or substituted with C.sub.1-4 -alkoxy, aryloxy or cycloalkyl or cycloalkylalkyl, when X is H and R.sup.2 is a methyl group.

Abstract: Heterocyclic dihydroxyquinoxaline compounds having the formula ##STR1## wherein R.sup.1 is hydroxy; and R.sup.5 and R.sup.6 together form a fused phenyl, cyclohexyl or pyridyl ring, which is optionally substituted with halogen or CN; and R.sup.7 and R.sup.8 independently are hydrogen, hydroxy, NO.sub.2, halogen, CN, CF.sub.3, C.sub.1-4 - acetyl, SO.sub.2 NHCOCO.sub.2 Et, SO.sub.2 NR'R' or SO.sub.2 R', wherein R' is hydrogen or C.sub.1-4 -alkyl; or R.sup.5 and R.sup.6 independently are hydroxy, hydrogen, NO.sub.2, halogen, CN, CF.sub.3, C.sub.1-4 -alkoxy, acetyl, SO.sub.2 NHCOCO.sub.2 Et, SO.sub.2 NR'R' or SO.sub.2 R', wherein R' is hydrogen or C.sub.1-4 -alkyl; and R.sup.7 and R.sup.8 together form a fused phenyl, cyclohexyl or pyridyl ring which is optionally substituted with halogen or CN.The invention also relates to a method of preparing the compounds, pharmaceutical compositions thereof, and their use.

Abstract: The ALDC derivative is produced by treating ALDC in an aqueous medium with glutaraldehyde in defined proportions with optional subsequent immobilization. The ALDC derivative which is used in beer fermentation exhibits a satisfactory stability at low pH.

Abstract: With a blood measuring apparatus according to the invention, which uses capillary tubes (16, 17), these can be filled with blood from a drop of blood of about 1 microliter. This small amount of blood can be produced by the pricking of a finger. The blood sample can then be placed in an apparatus (1) with equipment for the determination of the blood by transillumination. The tubes (16, 17) are mounted in an independent plate piece (6), and the tubes are secured in V-form so that the inlet ends lie closely up against each other. This provides the possibility of filling two tubes at the same time, and herewith the possibility of simultaneous determination of one's own blood and a comparison with blood in, for example, a prepared tube.

Abstract: New substituted urea compounds of formula I, or a pharmaceutically acceptable salt thereof: ##STR1## wherein A is ##STR2## wherein R.sup.3 is an oxadiazol, substituted with C.sub.1-8 -alkyl, C.sub.2-8 -alkenyl, C.sub.2-8 alkynyl, C.sub.3-7 cycloalkyl, benzyl, phenyl, C.sub.1-6 -alkoxy, C.sub.1-6 -alkylthio, amino or alkylamino;R.sup.2 is --H or lower alkyl;X is O or S;and R.sup.1 is a group of formula II, III, IV or V ##STR3## where n is 2 or 3, P is 1 or 2, q is 1 to 3, r is 1-3 and R.sup.4 and R.sup.5 are H,C.sub.1-7 alkyl or C.sub.3-6 cycloalkyl; and N-oxides thereof. The compounds are useful in pharmaceutical preparations for treating psychotic disorders, nausea and vomiting.

Abstract: An endoprotease preparation, comprising an isolated endoprotease is disclosed. The endoprotease is a serine protease, shows immunochemical idenity to a protease derived from Fusarium oxysporum DSM 2672, hydrolyzes the oxidized beta-chain of bovine insulin at the peptide bonds Arg (22)-Gly (23) and Lys (29)-Ala(30), has optimum activity towards casein in the pH range of 8.5-11.0 with nearly constant activity in the pH range, has optimum activity at a temperature of about 45.degree. C., and has an isolectric point of about 9.0-10.0. The preferred microorganism that the enzyme is isolated from is Fusarium oxysporum DSM 2672.

Abstract: The solubility of an enzyme in a liquid detergent can be improved by chemical modification of free primary amino groups in the enzyme while still preserving the enzyme activity. The modification preferably involves aldehyde treatment, acylation, or alkylation of the amino groups.

Abstract: The present invention relates to therapeutically active piperidine compounds, a method of preparing the same and to pharmaceutical compositions comprising the compounds. The novel compounds are useful as stimulants of the cognitive function of the forebrain and hippocampus of mammals and especially in the treatment of Alzheimer's disease, severe painful conditions and glaucoma.

Abstract: Novel thieno[2,3-b]pyrazine-2,3(1H,3H)-diones or tautomeric forms thereof of the formula (I) ##STR1## The compounds are useful in the treatment of neurological and psychiatric diseases.

Abstract: This invention relates to mutants or variants of Bacillus thuringiensis producing high amounts of active delta-endotoxins. The delta-endotoxins produced by the mutant or variant B. thuringiensis will have an activity directed towards the same pest insects as its parent B. thuringiensis delta-endotoxins, such as against lepidopterans (mutants from B. thuringiensis subsp. kurstaki or subsp. aizawai), dipterans (mutants from B. thuringiensis subsp. israelensis) or coleopterans (mutants from B. thuringiensis subsp. tenebrionis).

Abstract: Physically strong particles containing an immobilized enzyme are prepared for use in a fixed bed-column. The particles are prepared by adding a homopolymer of 1-amino ethylene or a copolymer of 1-amino ethylene and N-vinyl formamide to an aqueous medium containing an enzyme, adding a cross-linking agent to cross-link the enzyme and polymer and cause flocculation, and dewatering, sub-dividing and drying the resultant flocculant. Preferably, the enzyme is glucose isomerase and the cross-linking agent is glutaraldehyde, polyazetidine or diisocyanate.

Abstract: The heterocyclic compounds having the general formula I ##STR1## wherein R.sup.3 is ##STR2## wherein R' is H, C.sub.1-6 -alkyl or C.sub.3-7 -cycloalkyl; --B-- is --C(R").dbd.N-- or --N.dbd.C(R")-- wherein R" is a cyclic amine or --NR"'R"", wherein R"' and R"" independently are H,C.sub.1-6 -alkoxy, C.sub.3-7 -cycloalkyl or C.sub.1-6 -alkyl.The compounds are useful in psychopharmaceutical preparations as anticonvulsants, anxiolytics, hypnotics and in improving the cognitive function of the brain of mammals.

Abstract: A disubstituted piperazine compound having the formula ##STR1## wherein R.sup.1 is halogen, methoxy, C.sub.1-6 -alkyl or trifluoromethyl, and R.sup.2 is methyl or substituted C.sub.1-8 -alkyl, C.sub.3-8 -alkenyl or C.sub.3-8 -cycloalkyl, where substituents may be hydroxy-, keto- or oximino-groups in any position leading to a stable tertiary amine; or R.sup.2 is a straight or branched C.sub.1-8 -alkyl or C.sub.3-8 -alkenyl, which in any position may be substituted as above, but is terminally substituted with one of the following groups: cyano, optionally C.sub.1-4 -alkoxy-substituted C.sub.1-4 -alkoxy, dimethoxy, optiontionally substituted phenoxy, phosphonic acid, thienyl, furyl, oxazoline, isoxazole, oxadiazole, where the optional substitution is represented by C.sub.1-6 -alkyl or phenyl, provided that when cyano is the only substituent in R.sup.2, R.sup.2 must contain at least four carbon atoms, and pharmaceutically acceptable acid addition salts thereof.

Abstract: The transfer of a textile dye from a dyed fabric to another fabric during washing or rinsing is inhibited by adding an enzyme exhibiting peroxidase activity or an enzyme exhibiting a suitable oxidase activity to the wash liquor in which said fabrics are washed and/or rinsed. Peroxidase is produced extracellularly by some strains of Bacillus pumilus. The novel peroxidase preparation from B. pumilus is a microperoxidase, i.e. it contains hemopeptide as an active component. The preparation has improved stability at high temperature, at high pH and at high concentrations of hydrogen peroxide. It can be produced without undesired catalase activity.

Abstract: Thermally stable, positionally non-specific lipases native to Candida species of C. antartica, C. tsukubaensis, C. auriculariae, C. humicola, and C. foliarum, are isolated. The lipase of C. antarctica, is preferred. Two lipase activities are elaborated by C. antarctica. One lipase fraction being 43 kD in molecular weight, and of an isoelectric point of about 8.0 and has excellent thermostability. The other fraction being 33 kD in molecular weight and of an isoelectric point of about 6.0 and has high retention of residual activity at pH 10.