Abstract: A sample dispatcher is disclosed and is configured for individually introducing a plurality of portions of one or more sample fluids into a flow of a mobile phase of a liquid separation system. The liquid separation system is configured for separating compounds of the sample fluids and comprises a mobile phase drive configured for driving the mobile phase through a separation unit configured for separating compounds of the sample fluids in the mobile phase. The sample dispatcher comprises one or more sample reservoirs, each configured for receiving and temporarily storing a respective sample fluid portion or at least a part thereof, and a bypass channel.

Abstract: A device and a method of forming the same are disclosed. The device comprises a substrate and a thin film. The substrate is characterized by a first coefficient of thermal expansion. The thin film is attached to a surface of the substrate, and is characterized by a second coefficient of thermal expansion. The thin film includes first and second layers in states of compression, and a third layer in a state of tension, the third layer being positioned between the first and second layers. The thin film is in a net state of tension within a temperature range.

Abstract: A method for fragmenting a genome is provided. In certain embodiments, the method comprises: (a) combining a genomic sample containing genomic DNA with a plurality of Cas9-gRNA complexes, wherein the Cas9-gRNA complexes comprise a Cas9 protein and a set of at least 10 Cas9-associated guide RNAs that are complementary to different, pre-defined, sites in a genome, to produce a reaction mixture; and (b) incubating the reaction mixture to produce at least 5 fragments of the genomic DNA. Also provided is a composition comprising at least 100 Cas9-associated guide RNAs that are each complementary to a different, pre-defined, site in a genome. Kits for performing the method are also provided. In addition, other methods, compositions and kits for manipulating nucleic acids are also provided.

Abstract: A method of enriching for a fragment of a genome, as well as corresponding compositions and kits, are provided. In certain embodiments, the method comprises: (a) contacting a sample comprising fragmented DNA with a Cas9-gRNA complex comprising mutant Cas9 protein that has inactivated nuclease activity and a Cas9-associated guide RNA that is complementary to a site in the DNA, to produce a Cas9-fragment complex that comprises a fragment of the fragmented DNA; and (b) isolating the complex. In addition, other methods and compositions for Cas9/CRISPR-mediated nucleic acid manipulation are also provided.

Abstract: An ion detection system for detecting ions whose velocity varies during an operating cycle. The ion detection system includes a dynode electron multiplier (e.g., a microchannel plate (MCP)) having a bias voltage input, and a bias voltage source to apply a bias voltage to the bias voltage input of the dynode electron multiplier. With a fixed bias voltage applied to its bias voltage input, the dynode electron multiplier has a gain dependent on the velocity of ions incident thereon. The bias voltage applied by the bias voltage source to the bias voltage input of the dynode electron multiplier varies during the operating cycle to reduce the dependence of the gain of the dynode electron multiplier on the velocity of the ions incident thereon.

Abstract: A reagent for oligonucleotide synthesis or purification, wherein the reagent has a structure of: X—C—L—H??(Formula A) wherein X is a phosphoramidite group, an H-phosphonate group, an acetal group, or an isocyanate; C is a direct bond or a cleavable adaptor represented by —Ca—Cb—; L is a hydrocarbyl chain; and H is a terminal alkyne or an activated cyclooctyne. The reagent of Formula (A) can be used in the synthesis and purification of oligonucleotides.

Abstract: The present invention relates to methods and systems for detecting leaks in a head space sampling device. One exemplary method for detecting leaks in a head space sampling device includes establishing fluid communication between a head space and a pressurization gas conduit. Gas pressure and flow rate are monitored within the pressurization gas conduit during pressurization of the head space. Changes in monitored gas pressure and flow rate are used to evaluate whether a leak exists within the head space sampling device or the vial containing the head space. One exemplary head space sampling device includes a conduit for receiving a pressurization gas, flow and pressure sensors for measuring gas flow and pressure within the conduit, a ventilation valve, a pressure valve for controlling gas flow through the conduit, and a controller for processing and controlling pressure and flow through the conduit.

Abstract: The present invention discloses methods of using DNA polymerase fusions at high pH in PCR, DNA sequencing and mutagenesis protocols.

Abstract: Polyamide sample preparation tubes with a hollow polyamide tube and an extraction medium contained within the tube are utilized in the sample preparation devices. The sample preparation tubes are substantially inert and show little tendency to dissolve or leach contaminants into nonaqueous liquids. The polyamide tubes may be used in preparing samples for analytical procedures such as GC, GC/MS, LC or LC/MS.

Abstract: A vacuum pump and a method of controlling the operation of the vacuum pump employ an expansion chamber to reduce a pressure differential between an inlet side and an exhaust side of the pump. In particular, the expansion chamber may be used to rid the pump head of the pump of a pocket of relatively high pressure fluid that accumulates behind a poppet valve disposed over an exhaust opening. The pump itself may be used to create a level of vacuum pressure in the expansion chamber. Alternatively, a piston or diaphragm may be used. In either case, the expansion chamber is isolated from the ambient and interior of the pump head once a level of vacuum pressure is produced in the expansion chamber. Then the exhaust outlet is redirected from the ambient to the interior of the expansion chamber.

Abstract: An oscilloscope current probe system includes a probe amplifier unit, a probe head identifier, and first and second probe heads interchangeably connectable to the probe amplifier unit. Each probe head has a respective electrically-readable type identifier, a respective current input to receive a current to be measured, a respective internal sensing resistor in connected series with the current input, and a respective output at which a measurement voltage across the sensing resistor is output. The first and second probe heads differ in the resistance of their sensing resistors and the way their type identifiers read. The probe amplifier unit includes a differential amplifier to amplify the measurement voltage output by the probe head connected thereto. The probe head identifier is to read the type identifier of the probe head connected to the probe amplifier unit.

Abstract: An oscilloscope system includes a current probe, a processing unit and a display unit. The current probe includes a high and low gain signal paths for receiving simultaneously a signal of interest from a device under test (DUT), and to output amplified small and large current portions of the signal of interest, respectively. The processing unit receives first and second digitized data corresponding to the amplified small and large current portions output by the high and low gain signal paths, respectively, and processes the first and second digitized data for display as zoomed-in and zoomed-out waveform data, respectively. The display unit displays simultaneously a zoomed-in waveform in a first display window, based on the zoomed-in waveform data, and a zoomed-out waveform in a second display window, based on the zoomed-out waveform data, using the same time scale. The small current portion is less than a predetermined threshold.

Abstract: A probe for a measurement instrument comprises an input terminal configured to receive an input signal from a device under test (DUT), an output terminal configured to transmit an output signal to a measurement instrument, and a clamping circuit disposed in a signal path between the input terminal and the output terminal and configured to clamp an internal probe signal between an upper clamping threshold and a lower clamping threshold to produce the output signal, wherein the clamping circuit operates with substantial gain and amplitude linearity throughout a range between the upper clamping threshold and the lower clamping threshold.

Abstract: Methods, tools, systems and computer readable media for compliance testing instrumentation and/or software. Data from one or more analytical instruments and/or software is converted to a technology-neutral format, which is independent of instrument type, instrument model, instrument manufacturer and data type of the analytical instrument or software from which the data was outputted. Calculations are performed on the converted data to produce one or more outputs, and then selection is made from the one or more outputs to populate a final report, wherein the one or more outputs are standardized and are directly comparable to outputs resultant from carrying out the method on another set of one or more other analytical instruments and/or software, irrespective of manufacturer or model of the other analytical instruments and/or software.

Abstract: A multi-band frequency multiplier configured to generate frequencies and multiplied frequencies in an integrated system. The multi-band frequency multiplier includes a multi-band multiplier core with a multiplier core differential amplifier configured to receive a multiplier input signal. A switchable load impedance connects to the multiplier core differential amplifier, and includes n multiplier sections. Each multiplier section includes a section impedance and a section switch. The multiplier core differential amplifier generates an output signal having a frequency substantially equal to k times the input frequency in a range of a selected one of n critical frequencies when a selected one of the section switches corresponding to the selected one of the n critical frequencies is triggered.

Abstract: Disclosed are porous-shell particles, methods of making the particles, and uses thereof. In one aspect, the porous-shell particles are superficially porous particles.

Abstract: A method of calibrating a test instrument comprises determining a first response of a calibration device on the test instrument over a first set of operating ranges, determining a derived second response of the calibration device on the test instrument over a second set of operating ranges based on the first response, measuring the second response of the calibration device on the test instrument over the second set of operating ranges, and determining correction factors of the test instrument for the second set of operating ranges based on a comparison between the measured second response and the derived second response.

Abstract: The fluorescence measurement system includes a host apparatus and a self-contained, multichannel optical cartridge. The host apparatus includes a well plate receptacle, and a stage having optical cartridge receptacles that are elongate along one axis of the well plate. The stage and the well plate receptacle are movable relative to one another in the direction of the other axis of the well plate. The optical cartridge engages with the optical cartridge receptacle and includes a linear array of optical assemblies each including an excitation light source, and an emission light detector to generate an intensity signal. The optical cartridge also includes, a memory to store calibration information for each optical assembly. The host apparatus additionally includes a processor to correct the intensity signal from each optical assembly of the optical cartridge using the calibration information received from the optical cartridge for the optical assembly.

Abstract: Ion guides for use in mass spectrometry (MS) systems are described. The ion guides are configured to provide a reflective electrodynamic field and a direct current (DC or static) electric field to provide ion beams that are more spatially confined with a comparatively large mass range.

Abstract: A method for displaying signal analysis data comprises performing a plurality of sweep operations upon a received signal. With the sweep operations, spectral analysis is performed upon a first frequency band of the received signal. Displays are rendered of data generated from the spectral analysis in the first band and a second band different from the first band simultaneously, and the displays in the first band and the second bands are updated according to the sweep operations.