Abstract: Provided herein is method comprising: contacting an initial RNA sample containing a population of different RNA molecules with a divalent cation and a set of DNAzymes that are designed to cleave multiple target RNAs in the initial sample, thereby producing a product RNA sample that comprises: a) uncleaved RNA molecules and b) cleaved RNA fragments that contain a 2?,3?-cyclic-phosphate and a 5? hydroxyl as the result of DNAzyme cleavage.

Abstract: The present invention provides compositions and improved methods for multi-site directed mutagenesis and DNA shuffling. The present compositions and methods provide increased mutation frequency and increased number of transformants which allow one to sequence only a few clones in order to identify the correct mutants and to obtain the desired mutant by screening large number of transformants in a short time. Moreover, the inclusion of FEN-1, PEF and optimized buffer and cycling conditions provided in the present invention should also facilitate random mutagenized library construction and the mutagenesis of large or difficult templates.

Abstract: A shear valve for use in a high performance liquid chromatography system, the shear valve comprising a first shear valve member and a second shear valve member, wherein at least one of the first and second shear valve members is adapted to be moved with respect to the other, one of the first and second shear valve members comprises a plurality of ports, and the other comprises at least one fluid path for fluidly coupling respective ones of the ports in dependency on a relative movement position of the first and second shear valve members with respect to each other, wherein the first shear valve member is at least partially coated with an adhesion-promoting layer and a diamond like carbon layer on the adhesion-promoting layer.

Abstract: A method of determining a leak in a pumping system includes determining a metered flow rate of a fluid through a first channel, determining a measured flow rate within the channel, and identifying an amount of a leak in the pumping system as the difference between the metered flow rate and the measured flow rate.

Abstract: In accordance with an embodiment of the present invention, there is provided a method of performing the western blot analytical technique, wherein the method comprises carrying out the following steps in the following order: a), pre-staining the proteins within a sample; b). separating the proteins using gel electrophoresis; c). analysing the separated proteins to determine the total protein load and/or at least one housekeeping protein load; d). transferring the proteins onto at least one membrane; e). probing the separated proteins to detect a target protein; and f). analysing the probed proteins to determine the target protein's molecular weight and/or load.

Abstract: An atomic force microscope (AFM) comprises a physical system and a controller comprising a plurality of digital filters and configured to control the physical system. The AFM is tuned by performing automatic loop shaping on a loop response defined by a frequency response of the physical system and a frequency response of the controller, and adjusting a gain of the controller according to a peak in a magnitude of the loop response.

Abstract: A method of calibrating a reconstructed signal from a plurality of sub-signals is provided. The method includes injecting a calibration signal having multiple tones into a received input signal; dividing the input signal into a first and second sub-signal, including an overlapping frequency band; performing a first frequency translation by converting frequency components of the second sub-signal; digitizing the first sub-signal and the frequency converted second sub-signal; performing a second frequency translation to reverse the first frequency translation to obtain a reconstructed second sub-signal; and quantifying impairments to the digital first sub-signal and reconstructed second sub-signal caused by differences in magnitude and phase of frequency components within the overlapping frequency band.

Abstract: A system comprises an oscilloscope and an oscilloscope probe having a status indicator. The status indicator is located on a probe head of the oscilloscope probe and is configured to convey information to a user to indicate at least one characteristic of the signal. In certain embodiments, the status indicator comprises a light or a screen to convey signal related information to the user.

Abstract: A vessel includes a cylindrical section coaxially disposed about a central axis of the vessel. The cylindrical section includes an inside vessel surface, an outside vessel surface opposing the inside vessel surface, an upper end region circumscribing a vessel opening, and a lower end region axially spaced from the upper end region. A bottom section is disposed at the lower end region. A flange is coaxially disposed about the central axis and extends radially outward from the outside vessel surface at the upper end region. The flange includes a bottom surface facing generally toward the lower end region and perpendicular to the inside vessel surface. A ring may be coaxially disposed about the central axis and provide the bottom surface. An annular shoulder may protrude from the outside vessel surface, and may be spaced from or integrally adjoined to the flange.

Abstract: A mass analyzer comprises a pair of planar electrode structures. The electrode structures are disposed opposite to each other, parallel to each other, and axially offset from each other. The electrode structures are configured to generate, in response to an applied voltage, a cylindrically-symmetric, annular electric field comprising an annular radially focusing central lens region surrounding an axis of symmetry, and an annular mirror region surrounding the annular radially focusing central lens region.

Abstract: The present invention is a process for making an inorganic/organic hybrid totally porous spherical silica particles by self assembly of surfactants that serve as organic templates via pseudomorphic transformation.

Abstract: In a mass spectrometer or gas chromatograph/mass spectrometer system, a conditioning gas such as, for example, hydrogen is added to condition or clean one or more components or regions of the mass spectrometer such as the ion source. The conditioning gas may be added upstream of the mass spectrometer such as, for example, into a sample inlet or a chromatographic column, or may be added directly into the mass spectrometer. The conditioning gas may be added off-line, when the mass spectrometer is not analyzing a sample, or on-line during sample analysis. When added on-line, the conditioning gas may be mixed with a carrier gas such as, for example, helium. In another embodiment, the conditioning gas also serves as the carrier gas through the column; another gas such as, for example, helium may be added to the carrier gas stream.

Abstract: A method of filtering a signal sampled by a sampler, for example, in an equivalent time oscilloscope includes applying a correction to an actual frequency response of the sampler, with respect to a reference frequency response, to a first frequency range of the sampled signal, and transitioning across a second frequency range of the sampled signal from the correction applied to the first frequency range to no correction of the actual frequency response of the sampler, the second frequency range being higher than the first frequency range. The method further includes compensating in a third frequency range of the sampled signal for excess gain incurred while applying the correction and transitioning from the correction to no correction in the first and second frequency ranges, respectively, so that statistics of asynchronous components of the sampled signal are preserved, the third frequency range being higher than the second frequency range.

Abstract: A drive mechanism for back pressure regulator used in liquid chromatography, supercritical fluid chromatography, or supercritical fluid extraction allows very fine automated control over a very wide range of pressures by combining a linear actuator compressing a spring, pushing a pin. The nozzle assembly of the regulator comprises a flow through chamber containing a diaphragm and a seat, in which the pin pushes the diaphragm against the seat, together with an upstream pressure sensor and electronic feedback control to the motor of the actuator. The BPR of the embodiments exhibits high pressure stability and extremely low pressure noise, even at moderate to high pressures. The exemplary BPR can be use at either constant pressure or to generate pressure programs where the pressure is varied versus time. Further, the nozzle assembly has a field-replaceable head, requiring no mechanical adjustment on replacement.

Abstract: A system and method for determining the performance of a channel emulator includes an input signal, at least one transmitter configured to receive the input signal, the at least one transmitter coupled to at least one receiver through the channel emulator, the at least one transmitter configured to transmit the input signal through the channel emulator, the at least one receiver configured to receive a faded receive signal. The system also comprises a signal processor configured to receive a processor signal and the faded receive signal, the signal processor configured to correlate the processor signal and the faded receive signal to develop a correlated signal that represents a channel impulse of the channel emulator. The channel impulse of the channel emulator is used to extract at least one channel coefficient that reflects the performance of the channel emulator.

Abstract: Provided are improved processes and apparatuses for solid phase oligonucleotide synthesis, the improvements comprising carrying out detritylation of the nascent oligonucleotide using a composition of an organic solvent, a protic solvent and a selected concentration, or selected concentration range, of water. In some embodiments, an oligonucleotide synthesis apparatus includes means for adding water to a detritylation reagent. In some embodiments, an apparatus can include a water detector for analyzing the water concentration of a detritylation reagent to be reacted with a nascent oligonucleotide. An apparatus can comprise a feedback loop to control the concentration of water at the point of the detritylation reaction and/or to control the detritylation reaction time. The apparatuses and methods reduce batch-to-batch variations in the manufacture of oligonucleotides immobilized on the surface of various substrates.

Abstract: Aspects of the invention provide systems and methods for high-throughput screening of fluidic samples. In some embodiments, two chromatography columns are utilized in series. The first chromatography column can have a high affinity for phosphorylated compounds while the second chromatography column has a high affinity for one or more analytes of interest.

Abstract: A magnetic resonance imaging (MRI) apparatus comprises a plurality of cylindrical electromagnetic coils arranged in a coaxial configuration around a sample region. The coils are used to capture resonance signals from a sample at different times according to a geometric echo effect. The measurements can then be combined to produce an MRI signal.

Abstract: A system for performing analog-to-digital conversion comprises a sampling unit that generates multiple digital samples from an analog input signal at each recurrence of a periodic interval, and a processing unit that combines the digital samples to produce a digital output signal. In certain embodiments, the sampling unit comprises multiple analog-to-digital converters arranged in parallel, and the processing unit comprises a digital signal processor that detects outliers in the digital samples and averages any non-outliers among the digital samples to generate the digital output signal.

Abstract: A current probe enabling measurement of current of a signal in a circuit includes a ferrite core defining a gap, a wire wrapped around the ferrite core and a magnetic field sensor. The wire is configured to receive the signal from the circuit, where the current of the signal flowing in the wire generates a magnetic field in the ferrite core. The magnetic field sensor is positioned in the gap of the ferrite core, the magnetic field generated in the ferrite core flowing through the magnetic field sensor, which produces a voltage proportional to an intensity of the magnetic field. The current is measured based on the voltage produced by the magnetic field sensor.