Abstract: A container assembly is disclosed including an outer container, a hollow inner member, and a closure. The outer container has a closed bottom, an open top, and a sidewall extending therebetween. The hollow inner member is disposed within the outer container and has an inner surface defining at least one capillary channel. The inner member includes a first end adjacent to the open top of the outer container and has an outer periphery seated against the sidewall of the outer container. The closure has a proximal end and a distal end. The closure proximal end is seated at least partially within the first end of the inner member to seal the outer container and inner member and define a fluid collection chamber. The closure distal end defines a recessed area shaped to direct fluid under capillary action to the at least one capillary channel in the inner member.

Abstract: An injection monitoring device includes a syringe having a barrel configured to contain a medicament and a plunger configured to be displaced linearly into the interior of the barrel to dispense the medicament, a flange positioned between a proximal end and a distal end of the injection monitoring device and configured to be gripped during performance of the injection, and one or more force sensors positioned to detect data associated with an amount of force applied to the injection monitoring device during performance of an injection.

Abstract: Flush syringe assemblies are described herein. Such flush syringe assembly may include a barrel including a side wall defining a chamber, a collar mounted on a distal wall of the barrel and surrounding an elongate tip. A cap may be attached to the collar via a hinge. The flush syringe assembly may also include a cap attached to the collar via a hinge. A ring may be disposed around the collar with a locking element that engages with a corresponding mating locking projection on the cap. The cap may include a slanted surface. The slanted surface of the cap may include one or more ribs made of an elastomeric material.

Abstract: Disclosed herein are methods, compositions, and kits for barcoding nucleic acids. For example, particles for barcoding are provided, where the particle comprises: a first plurality of oligonucleotide barcodes each comprising a target-binding region, and a second plurality of oligonucleotides each comprising a target-specific target-binding region.

Abstract: An infusion set includes a base (221) and a flexible catheter (242) movable from a first catheter position disposed substantially entirely within the base (221) to a second catheter position in which a free end of the catheter (242) is disposed externally of the base (221). An introducer needle (234) is located within the catheter (242) and is movable between a first introducer needle position disposed substantially entirely within the base (221) and a second introducer needle position in which a free end of the introducer needle (234) is disposed externally of the base (221). A spring (281) is activated to move the catheter (242) from the first to the second catheter position and the introducer needle (234) from the first to the second introducer needle position to facilitate insertion of the catheter (242).

Abstract: A medicament delivery system (1) and method for determining an occlusion comprising a reservoir (4) that carries a medicament, a pump (3) connected to the reservoir (4) that draws the medicament out of the reservoir (4) for medication delivery, and an occlusion sensor (600) connected to the reservoir (4) that senses whether an occlusion is present wherein the occlusion sensor (600) is secured to an outer surface of the reservoir (4), the occlusion sensor (600) provides resistance output (630) to control electronics (8), and the control electronics (8) determines whether an occlusion is present based on the resistance output (630). The occlusion sensor (600) can be a strain gauge bonded onto a mid-section of an outer surface of a flexible reservoir (4) to provide a sensitive strain reading.

Abstract: An automated apparatus can provide pre-analytical processing of samples, racking and forwarding to an adjacent analyzer for analysis. The apparatus may have a controller that implements an auto-learn process to teach robotic handlers the locations within the workspace(s) of the apparatus. A robotic sample handler may include a sensor configured to generate a detection signal when in a near vicinity of a fiducial beacon in the workspace of the apparatus for biological sample preparation, preprocessing and/or diagnostic assay performed by one or more analyzers of the automated apparatus. The controller may control the robotic sample handler to conduct a search pattern so that a location of the fiducial beacon may be detected and thereafter calculated to obtain a more accurate location of the beacon. The calculated positions may then serve as a basis for the controlled movement of samples by the robot to and from locations of the workspace.

Abstract: Aspects of the disclosure include a system for performing cytometry that can be operated in three operational modes. In one operational mode, a fluorescence image of a sample is obtained by exciting one or more fluorophore(s) with a top-hat-shaped excitation beam with a plurality of beams that are radiofrequency shifted relative to one another. In another operational mode, a sample can be illuminated successively over a time interval by a laser beam at a plurality of excitation frequencies in a scanning fashion to generate an image of the sample from the detected fluorescence. In another operational mode, the system can be operated to illuminate a plurality of locations of a sample concurrently by a single excitation frequency (e.g., a horizontal extent), which is generated, e.g., by shifting the central frequency of a laser beam by a radiofrequency and detected fluorescence is used to analyze the sample.

Abstract: The invention relates to a device for vacuum stoppering a medical container. The device includes a main body defining an internal volume that has a variable pressure chamber configured to be connected to a vacuum pump. A stopper holder system is arranged in communication with the variable pressure chamber and is configured to receive and hold a stopper aligned with the direction of travel of a piston rod. A piston rod is moveable inside the internal volume of the main body along a longitudinal axis between a proximal rest position and a distal operative position wherein the piston rod pushes the stopper into the medical container. A container holder system provided in the main body, is arranged distally relative to the stopper holder system and in communication with the variable pressure chamber.

Abstract: A device for accommodating and magnetizing a tissue-penetrating medical device of various lengths, with or without a cover covering a portion or the entirety of the tissue-penetrating medical device, is disclosed including a sleeve member having an open proximal end, a distal end, an inner surface, an outer surface having a graduated injection depth gauge to indicate needle penetration depth when the cover is placed into a magnetizer, and a hollow body extending between the proximal end and the distal end to form a protective closure over a shaft of a tissue-penetrating medical device. A device having one or more magnetizing elements sectioned into a plurality of movable segments pivoting around an axis to accommodate needles with different lengths is also disclosed. Also disclosed is a device having one or more magnetizing means mounted on a movable element to magnetize needles of various lengths.

Abstract: Aspects of the present disclosure include a method for sorting cells of a sample based on an image of a cell in a flow stream. Methods according to certain embodiments include detecting light from a sample having cells in a flow stream, generating an image mask of a cell from the sample and sorting the cell based on the generated image mask. Systems having a processor with memory operably coupled to the processor having instructions stored thereon, which when executed by the processor, cause the processor to generate an image mask of a cell in a sample in a flow stream and to sort the cell based on the generated image mask are also described. Integrated circuit devices (e.g., field programmable gate arrays) having programming for generating an image mask and for determining one or more features of the cell are also provided.

Abstract: Disclosed herein include systems, methods, kits, and compositions for labeling nuclear target-associated DNA in a cell. Some embodiments provide digestion compositions comprising a DNA digestion enzyme and a binding reagent capable of specifically binding to the nuclear target. Some embodiments provide conjugates comprising a transposome and a binding reagent capable of specifically binding to a nuclear target. The transposome can comprise a transposase (e.g., Tn5 transposase), a first adaptor having a first 5? overhang, and a second adaptor having a second 5? overhang. The methods can comprise contacting a permeabilized cell comprising a nuclear target associated with dsDNA, such as genomic DNA (gDNA), with the compositions provided herein to generate a plurality of nuclear target-associated dsDNA fragments (e.g., nuclear target-associated gDNA fragments) each comprising the one or two single-stranded overhangs.

Abstract: A nest and tub arrangement for the storage of medical device components including a tub having a plurality of guide elements extending from at least one surface of the tub and into the interior of the tub, and at least one nest having a plurality of receptacles for the storage of a plurality of medical device components therein. The at least one nest is sized and configured for placement within the tub such that the plurality of guide elements align and removably retain the at least one nest within the tub.

Abstract: A valve actuator that moves in a catheter assembly between a first position where a valve is closed and a second position where the valve is open, the valve actuator including a shaft portion at a distal end of the valve actuator that is configured to pierce the valve, a mating portion at a proximal end of the valve actuator that is configured to engage a Luer device, a diameter reduction region that connects the shaft portion and the mating portion, and a plurality of windows that extend through the valve actuator for flushing fluid, the plurality of windows being disposed in the diameter reduction region, wherein each of the plurality of windows does not extend a full length of the diameter reduction region.

Abstract: A catheter system may include a catheter assembly, which may include a catheter adapter and a catheter extending distally from a distal end of the catheter adapter. The catheter system may also include a needle assembly, which may include a needle hub and an introducer needle. The needle hub may include: a distal end coupled to the proximal end of the catheter adapter; a proximal end; a wall disposed between the distal end of the needle hub and the proximal end of the needle hub; and a flashback chamber proximate the wall. The introducer needle may be press-fit within a through-hole of the wall. The catheter system may also include a plug coupled to the proximal end of the needle hub. A blood collection device may be coupled to a proximal end of the plug to collect blood from a patient.

Abstract: Devices, systems and methods are provided to securely and wirelessly connect a medical device to a controller or smartphone with medical device control app, and to wirelessly connect the controller or smartphone with medical device control app to other devices providing additional integrated disease management (IDM) functions. At least two different wireless communication protocols are used depending on level of security needed, with greater security needed for exchange of medical device control operation commands and related data between the medical device and its controller or smartphone with medical device control app to avoid nefarious or unintended changes in medical device control. Exchanges of historical data or notifications relating to the medical device and requiring less security can be transmitted to the controller and/or the smartphone or other IDM devices using a different and more commonly used wireless communication protocol that may afford less security.

Abstract: Disclosed herein include systems, methods, compositions, and kits for performing targeted single cell mRNA sequencing assays. Capture oligonucleotides capable of specifically binding to target nucleic acid species are provided in some embodiments. Depletion oligonucleotides capable of specifically binding to undesirable nucleic acid species are provided in some embodiments. The method can comprise random priming and extension of barcoded transcripts followed by one or more enrichment and/or depletion steps using said capture oligonucleotides and/or depletion oligonucleotides. Immune repertoire profiling methods are also provided in some embodiments.

Abstract: Inserts can be formed with elution characteristics to cause the inserts to elute an antimicrobial agent when subject to a fluid within a medical device. An insert can be formed with a desired geometry to allow the insert to be compression fit within a medical device to prevent the insert from moving or becoming dislodged once inserted into the medical device. The material may also be hygroscopic so that the insert swells when subject to a fluid thereby enhancing the compression fit of the device within the medical device. In some cases, the material can be reinforced using an internal structure. Inserts can be formed in many ways including by casting, thermoforming, or extrusion. In some cases, the inserts can be formed using a peel-away sleeve or material. The peel-away sleeves can be formed of a non-sticky material which facilitates removal of the inserts once the inserts have cured.

Abstract: Disclosed herein include systems, methods, compositions, and kits for whole transcriptome analysis (WTA) with random priming and extension (RPE). The RPE-based WTA method can comprise hybridizing random primers with a plurality of first strand barcoded polynucleotides associated with a solid support and extending the random primers to generate a plurality of extension products. The method can comprise amplifying the plurality of extension products to generate a sequencing library.

Abstract: A medical injection system adapted for a single-handed injection, the medical injection device comprising a gas compartment configured to contain a gas, the gas compartment comprising a piston, a reservoir intended to contain a drug composition, the reservoir having a proximal portion and a distal portion provided with a fluid exit configured to receive an injection needle, wherein the piston can move proximally between a distal position defining a first volume of the gas compartment to a proximal position defining a second volume of the gas compartment smaller than the first volume, and wherein the gas compartment is in gas communication with the reservoir in order to force the drug composition through the fluid exit, when the piston is moved proximally.