Abstract: We describe pH-sensitive endosomolytic polymers, delivery particles containing pH-sensitive endosomolytic polymers. The described particles are capable of delivering polynucleotides to cells from the peripheral circulation with subsequent release from endosomes. The endosomolytic polymers are inactive outside the cell but disrupt membranes upon exposure to an acidified endosomal compartment.
Type:
Application
Filed:
September 19, 2006
Publication date:
February 15, 2007
Applicant:
MIRUS BIO CORPORATION
Inventors:
David Rozema, Darren Wakefield, Jon Wolff, Vladimir Budker, Tatyana Budker, Sean Monahan, Vladimir Trubetskoy, James Hagstrom, Aaton Loomis, Paul Slattum
Abstract: Intermolecular mutual recognition between pathogenic matter, such as allergen group or disease-causing microbe, capable of functioning at the attachment thereof to living organism and a living organism is inhibited by the use of pectins or nucleic acids.
Abstract: A hydraulic control device for an industrial machine, which can efficiently control a high flow rate of a pressure oil by a simple hydraulic instrument configuration, is provided.
Abstract: Disclosed is a process for transfecting genetic material into a mammalian cell to alter endogenous properties of the cell. The process comprises designing a polynucleotide for transfection. Then the polynucleotide is inserted into a mammalian vessel such as a tail vein or artery. Prior to insertion, subsequent to insertion, or concurrent with insertion the permeability of the vessel is increased thereby the genetic material is delivered to the parenchymal cell altering endogenous properties of the cell.
Type:
Grant
Filed:
June 20, 2003
Date of Patent:
December 12, 2006
Assignee:
Mirus Bio Corporation
Inventors:
Sean D. Monahan, Jon A. Wolff, Paul M. Slattum, James E. Hagstrom, Vladimir G. Budker, David B. Rozema
Abstract: Processes are described for obtaining high levels of gene expression in primates after injection of nucleic acid to the liver via the lumen of the hepatic vein. The described process results in high level of gene expression with transient increases in liver enzymes.
Type:
Grant
Filed:
December 5, 2002
Date of Patent:
December 5, 2006
Assignee:
Mirus Bio Corporation
Inventors:
Jon A. Wolff, Julia Hegge, James E. Hagstrom, Vladimir G. Budker
Abstract: A system relating to the delivery of desired compounds (e.g., drugs and nucleic acids) into cells using pH-sensitive delivery systems. The system provides compositions and methods for the delivery and release of a compound to a cell.
Type:
Grant
Filed:
July 15, 2003
Date of Patent:
November 21, 2006
Assignee:
Mirus Bio Corporation
Inventors:
Jon A. Wolff, James E. Hagstrom, David B. Rozema, Sean D. Monahan, Vladimir G. Budker
Abstract: Described is a deliverable composition with low toxicity comprising an amphipathic compound, a polycation, and a siRNA. The composition may be used in the process of delivering a siRNA to an animal cell or more particularly, a mammal cell.
Type:
Grant
Filed:
May 28, 2002
Date of Patent:
September 5, 2006
Assignee:
Mirus Bio Corporation
Inventors:
David L. Lewis, James E. Hagstrom, Hans Herweijer, Aaron G. Loomis, Sean D. Monahan, Jon A. Wolff
Abstract: An polyampholyte is utilized in a condensed polynucleotide complex for purposes of nucleic acid delivery to a cell. The complex can be formed with an appropriate amount of positive and/or negative charge such that the resulting complex can be delivered to the extravascular space and may be further delivered to a cell.
Type:
Grant
Filed:
July 26, 2004
Date of Patent:
August 29, 2006
Assignee:
Mirus Bio Corporation
Inventors:
David B. Rozema, Vladimir G. Budker, James E. Hagstrom, Vladimir Trubetskoy, Jon A. Wolff, Sean D. Monahan, Paul M. Slattum
Abstract: An polyampholyte is utilized in a condensed polynucleotide complex for purposes of nucleic acid delivery to a cell. The complex can be formed with an appropriate amount of positive and/or negative charge such that the resulting complex can be delivered to the extravascular space and may be further delivered to a cell.
Type:
Grant
Filed:
January 28, 2005
Date of Patent:
August 29, 2006
Assignee:
Mirus Bio Corporation
Inventors:
Vladimir S. Trubetskoy, James E. Hagstrom, Vladimir G. Budker, Jon A. Wolff, David B. Rozema, Sean D. Monahan
Abstract: Polyampholyte are able to condense nucleic acid to form small complexes which can be utilized in the delivery of nucleic acid to mammalian cells. The polyampholytes can be formed prior to interaction with nucleic acid or they can be formed in the presence of nucleic acid. Stabilized polycation/nucleic acid complexes can be modified to reduce the positive charge of the polycation and add targeting ligands without destabilizing the complex. The resultant particles retain their small size and are more effective in delivery of nucleic acid to cells in vivo.
Type:
Grant
Filed:
January 28, 2005
Date of Patent:
August 22, 2006
Assignee:
Mirus Bio Corporation
Inventors:
Darren H. Wakefield, David B. Rozema, Jon A. Wolff, Vladimir Trubetskoy, James E. Hagstrom, Vladimir G. Budker, Jason Klein, So Wong
Abstract: A complex is described that is deliverable to a cell comprising inserting a nucleic acid or other cargo into a reverse micelle. The reverse micelle has the property to compact the nucleic acid for easier delivery.
Type:
Grant
Filed:
July 25, 2003
Date of Patent:
August 15, 2006
Assignee:
Mirus Bio Corporation
Inventors:
Sean D. Monahan, Jon A. Wolff, Paul M. Slattum, James E. Hagstrom, Vladimir G. Budker
Abstract: We describe compounds that bind to and are internalized by hepatocytes. Association of these compounds to other molecules or complexes can be used to target the molecules or complexes to hepatocytes in vivo or in vitro.
Abstract: A method of forming polymers in the presence of nucleic acid using template polymerization. Also, a method of having the polymerization occur in heterophase systems. These methods can be used for the delivery of nucleic acids, for condensing the nucleic acid, for forming nucleic acid binding polymers, for forming supramolecular complexes containing nucleic acid and polymer, and for forming an interpolyelectrolyte complex.
Type:
Grant
Filed:
December 5, 2001
Date of Patent:
May 23, 2006
Assignee:
Mirus Bio Corporation
Inventors:
Jon A. Wolff, James E. Hagstrom, Vladimir G. Budker, Vladimir S. Trubetskoy, Paul M. Slattum, Lisa J. Hanson
Abstract: We describe a process for generating multilayer particles comprising condensing a polymer with an oppositely charged polymer to form a particle and sequentially adding oppositely charged polymers to the particle forming at least three layers of polymers. The process is used to form a composition for delivering a biologically active compound to a cell.
Type:
Grant
Filed:
March 8, 2004
Date of Patent:
May 16, 2006
Assignee:
Mirus Bio Corporation
Inventors:
Vladimir Trubetskoy, Jon A. Wolff, James E. Hagstrom, Vladimir G. Budker, So Chun Wong, Jason Klein
Abstract: A polyampholyte is utilized in a condensed polynucleotide complex for purposes of nucleic acid delivery to a cell. The complex can be formed with an appropriate amount of positive and/or negative charge such that the resulting complex can be delivered to the extravascular space and may be further delivered to a cell.
Type:
Grant
Filed:
March 11, 2002
Date of Patent:
April 25, 2006
Assignee:
Mirus Bio Corporation
Inventors:
Vladimir S. Trubetskoy, James E. Hagstrom, Vladimir G. Budker, Jon A. Wolff, David B. Rozema, Sean D. Monahan
Abstract: A method of forming polymers in the presence of nucleic acid using template polymerization. Also, a method of having the polymerization occur in heterophase systems. These methods can be used for the delivery of nucleic acids, for condensing the nucleic acid, for forming nucleic acid binding polymers, for forming supramolecular complexes containing nucleic acid and polymer, and for forming an interpolyelectrolyte complex.
Type:
Grant
Filed:
January 12, 2004
Date of Patent:
April 4, 2006
Assignee:
Mirus Bio Corporation
Inventors:
Vladimir Trubetskoy, Jon A. Wolff, Paul M. Slattum, Lisa Hanson, Vladimir G. Budker, James E. Hagstrom
Abstract: An process for the reversible modification of membrane interaction of a compound is described. Modification of membrane interaction can be used to facilitate delivery of molecules to cells in vitro and in vivo. The described modifiers, which are used to reversibly inactivate the membrane active compounds, can also be utilized as cross-linkers or to reverse the charge of a molecule.
Type:
Grant
Filed:
May 23, 2003
Date of Patent:
March 28, 2006
Assignee:
Mirus Bio Corporation
Inventors:
David B. Rozema, Darren Wakefield, Jon A. Wolff, Kirk Ekena, James E. Hagstrom
Abstract: Disclosed is a process for providing for expression of an exogenous nucleic acid in an extravascular parenchymal cell of a mammal. The nucleic acid is inserted into a vessel of a mammal and the permeability of the vessel is increased. Increasing permeability of the vessel allows delivery of the nucleic acid to an extravascular parenchymal cell.
Type:
Grant
Filed:
December 10, 2004
Date of Patent:
March 21, 2006
Assignee:
Mirus Bio Corporation
Inventors:
Jon A. Wolff, Vladimir G. Budker, James E. Hagstrom, Julia Hegge, Hans Herweijer
Abstract: A method of forming polymers in the presence of nucleic acid using template polymerization. Also, a method of having the polymerization occur in heterophase systems. These methods can be used for the delivery of nucleic acids, for condensing the nucleic acid, for forming nucleic acid binding polymers, for forming supramolecular complexes containing nucleic acid and polymer, and for forming an interpolyelectrolyte complex.
Type:
Grant
Filed:
December 5, 2001
Date of Patent:
November 1, 2005
Assignee:
Mirns Bio Corporation
Inventors:
Jon A. Wolff, James E. Hagstrom, Vladimir G. Budker, Vladimir S. Trubetskoy, Paul M. Slattum, Lisa J. Hanson