Abstract: New and improved hydrogels and methods associated therewith including their use for cell cultures and various other biomedical applications are disclosed. Because the instant invention does not rely on two dimensional surface area for cell growth but uses a three dimensional model, the hydrogels as disclosed herein have improved properties that allow for improved cell growth, easier and better harvesting and isolation of cells and small molecule products such as exosomes, as well as higher yields that can be achieved less expensively.

Abstract: Provided herein are antibodies and chimeric priming receptors that bind PSMA and antibodies and chimeric antigen receptors that bind CA9. Also provided are systems of chimeric priming receptors that bind PSMA and chimeric antigen receptors that bind CA9, cells expressing such systems, and methods of use thereof.

Abstract: A method for dynamic evolution and/or adaptation and monitoring of characteristics in living cells is provided, wherein the method may be performed at a microfluidic-enabled cell-culture device comprising pneumatic layer for directing flow of fluid to a plurality of individually addressable wells, and one or more sensors configured to detect data regarding environments inside one or more of the plurality of wells. The method may involve culturing a population of cells in a first well of the plurality of wells, perturbing one or more characteristics of an environment in the first well following the culturing of the population of cells, monitoring one or more characteristics of the population of cells in the first well, and removing all or part of the evolved/adapted population of cells from the first well.

Abstract: The present disclosure is directed to methods of treating or preventing RNA virus infections and retroviral diseases, such as HIV and AIDS, comprising administering to a subject in need an effective amount of (a) a capsid inhibitor and (b) an adenosine derivative disclosed herein. Compositions comprising an effective amount of an adenosine derivative and an effective amount of a capsid (CA) inhibitor are also provided.

Abstract: Provided herein are compounds, compositions and methods of using the compounds and compositions for the treatment of diseases modulated, as least in part, by AhR. The compounds are represented by formulae: wherein the letters and symbols X1, X2, Z, R1b, R1c, R1d, R1e, R2a, R2b, R2c and R2d have the meanings provided in the specification.

Abstract: Disclosed herein are compositions and methods for treating cardiomyopathies and certain cancers using drugs that target BANCR. In an aspect siRNAs that target exons 3 and 4 of BANCR are used.

Abstract: The invention relates generally to multispecific polypeptides that bind at least CD3, a second antigen, and a receptor of a T cell, such as a costimulatory receptor or an inhibitory receptor, in which the multispecific polypeptide constructs are able to engage CD3. In some embodiments, the multispecific polypeptide constructs bind a costimulatory receptor and provide costimulatory binding activity. In some embodiments, the multispecific polypeptide constructs bind an inhibitory receptor and block inhibitory activity. In some aspects, the multispecific polypeptides have constrained CD3 binding and bind to or engage CD3 only upon binding to the second antigen, such as a tumor associated antigen. In some embodiments, the multispecific polypeptides contain cleavable linkers that, when cleaved, result in dual effector functions. Also provided are methods of making and using these multispecific polypeptides in a variety of therapeutic, diagnostic and prophylactic indications.

Abstract: This invention provides rho-associated protein kinase (ROCK) inhibiting compounds for therapeutic applications as described further herein.

Abstract: The invention comprises methods and compositions for treating autoimmune diseases with engineered immune cells including cytotoxic T cells and natural killer (NK) cells. The engineered immune cells comprise a chimeric antigen receptor (CAR). Methods of making the engineered cells, methods of administration and treatment regimens are also disclosed.

Abstract: A method of treating hypertension includes an implantable stimulator generating stimulation sessions at a duty cycle that is less than 0.05 and applying the stimulation sessions by way of the central electrode and the annular electrode to a location associated with the hypertension and within a patient.

Abstract: Disclosed herein are methods for preventing or treating non-arteritic anterior ischemic optic neuropathy in a subject by administering to the subject a nucleic acid molecule comprising a nucleic acid sequence encoding OCT4, a nucleic acid sequence encoding SOX2, and a nucleic acid sequence encoding KLF4.

Abstract: Provided herein are novel thermostable phycobiliproteins. These proteins may be stabilized by the introduction of disulfide bonds which stabilize the protein. Modified cells expressing these thermostable phycobiliproteins and methods of making them are also provided.

Abstract: The present invention provides a method of treating a nucleotide repeat expansion disorder comprising delivering a pair of engineered nucleases, or genes encoding engineered nucleases, to the cells of a patient such that the two nucleases excise the nucleotide repeat responsible for the disease permanently from the genome. The invention provides a general method for treating nucleotide repeat expansion disorders and engineered nucleases suitable for practicing the method. The invention further provides vectors and techniques for delivering engineered nucleases to patient cells.

Abstract: Provided herein are methods of generating gonadal cell populations such as ovarian somatic cells from pluripotent stem cells. Also included are gonadal cell populations, as well as intermediate cell populations generated therein.

Abstract: Improvements to vaccines against RSV include G protein CCD portions complexed with mAb that block interaction with CX3C-R and modified forms of mAb to prolong serum half-life, as well as inhalable vaccines.

Abstract: Aspects of the present invention relate to a networked cell culture incubator and to methods for operating such an incubator. In one aspect, the cell culture incubator includes a network interface for communicating with a source of parameter data utilized successfully by other incubators. The incubator receives appropriate parameter data and conducts the incubation process as prescribed by the parameter data so as to provide an improved environment for cell culture growth. The incubator may share its own parameter data with the data source for use by other incubators. The incubator and data source may share other forms of data as well.

Abstract: Provided herein are wearable harnesses for securing and positioning an ultrasound device on a subject. The wearable harnesses can be used to position an ultrasound transducer to a human body. The wearable harnesses may include two adjustable shoulder straps, an adjustable waist strap, a pocket for holding a power pack, and a pocket for holding the transducer. For example, the pocket for holding the transducer may be positioned over the subject's spleen such that the ultrasound transducer may be positioned over the subject's spleen. Provided herein is also a treatment unit to be used to the wearable harness, that includes an ultrasound transducer; and a mechanical lens comprising a plurality of concentric rings and configured to adjust ultrasonic waves emitted by the ultrasound transducer.

Abstract: Provided herein are a fusion protein comprising an interleukin-22 domain and an albumin binding domain, and a pharmaceutical composition thereof. Also provided herein are methods of their use for treating, preventing, or ameliorating one or more symptoms of an inflammatory disease.

Abstract: This invention provides rho-associated protein kinase (ROCK) inhibiting compounds for therapeutic applications as described further herein.

Abstract: Provided herein are VHH-containing polypeptides that bind ?? T-cells. Uses of the VHH-containing polypeptides are also provided.