Patents Assigned to CHINESE ACADEMY OF AGRICULTURAL SCIENCES
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Publication number: 20180295868Abstract: The present invention provides a flavor extract of low-temperature-pressed peanut cake which is extracted from low-temperature-pressed peanut cake. In the flavor extract, the content of pyrazines flavor substances is not less than 55%, and the content of the aldehydes substances is not more than 15%. The present invention also provides a method for improving the flavor of peanut oil by using the flavor extract of the low-temperature-pressed peanut cake. The flavor extract extracted from peanut cake according to the present invention, which contains 55% or more pyrazines flavor substances, can not only improve the flavor of low-temperature-pressed peanut oil significantly, but also retain the original quality and color of the low-temperature-pressed peanut oil when it is added to low-temperature-pressed peanut oil.Type: ApplicationFiled: September 6, 2017Publication date: October 18, 2018Applicant: Institute for Application of Atomic energy, Chinese Academy of Agricultural SciencesInventors: Qiang Wang, Hui Hu, Yunhua Liu, Lei Deng, Aimin Shi, Hongzhi Liu, Li Liu
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Patent number: 10053682Abstract: The present invention relates to the field of genetic engineering and hereditary engineering. The present invention discloses a ?-galactosidase (?-galactoside galactohydrolase, EC 3.2.1.23) mutant with high transglycosidase activity, which is obtained by single-site-saturation mutation of amino acid sequences of ?-galactosidase from Aspergillus candidus and Aspergillus oryzae, with own signal peptides removed. The transglycosidase activity of the mutant is over 15% higher than that of wild types. Meanwhile, the present invention also discloses a DNA molecule which encodes the mutant, a recombinant expression vector containing the DNA molecule, and a host cell expressing the DNA molecule. In addition, the present invention also provides a method for preparing ?-galactosidase mutant with high transglycosidase activity by using the recombinant expression vector and applications of the mutant, the DNA molecule, the recombinant expression vector and the host cell in preparation of ?-galactosidase.Type: GrantFiled: April 7, 2015Date of Patent: August 21, 2018Assignee: BIOTECHNOLOGY RESEARCH INSTITUTE, CHINESE ACADEMY OF AGRICULTURAL SCIENCESInventors: Wei Zhang, Yuhong Zhang, Bo Liu, Ning Sun, Jialin Zhang
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Patent number: 10041085Abstract: A plant type related protein, and a coding gene and an application thereof are provided. The protein is: (a) a protein consisting of the amino acid sequence of SEQ ID NO: 1; (b) a SEQ ID NO: 1-derived protein having substitution, deletion, and/or addition of an amino acid residue on the sequence of SEQ ID NO: 1, and related to the plant type and/or inactivation of a plant brassinolide type, or (c) a protein having more than 80% homology to the sequence of SEQ ID NO: 1 and related to a plant type and inactivation of a plant brassinolide type. The protein and its coding gene have very important value in improving crop production, improving the visual enjoyability of a green plant, implementing simple cultivation of a plant and improving the breeding efficiency, and has a broad prospective in genetic improvement of a plant, new variety cultivation and an application.Type: GrantFiled: April 9, 2013Date of Patent: August 7, 2018Assignee: Cotton Research Institute, Chinese Academy of Agricultural SciencesInventors: Fuguang Li, Zuoren Yang, Chaojun Zhang, Yufen Wang, Zhixia Wu, Chuanliang Liu, Xueyan Zhang, Ye Wang, Fenglian Li, Qianhua Wang, Wenqiang Qin, Depei Kong
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Patent number: 10024827Abstract: The present application relates to a method for simultaneously detecting four isomers of resveratrol in peanut by using ultra performance liquid chromatography during the operation procedure and accurately controlling the detection conditions. The method of the invention can achieve continuous sample injection, and perform sample analysis in batch. The detection time for each sample is only about 10 min, which greatly improves the detection efficiency. Further, the ultra performance liquid chromatography has high sensitivity and a low detection limit. The method of the invention adopts ethanol extraction under heating in the earlier stage of extraction, and controls the temperature of the extraction, so as to achieve effective extraction of four isomers of resveratrol, time saving, and efficient extraction and separation.Type: GrantFiled: December 18, 2017Date of Patent: July 17, 2018Assignee: Institute of Food Science and Technology, Chinese Academy of Agricultural SciencesInventors: Qiang Wang, Hui Hu, Xiaoyong Xia, Aimin Shi, Hongzhi Liu, Li Liu, Bo Jiao
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Publication number: 20180163215Abstract: Provided is an amino acid optimization of a functional motif on an IrrE protein of a Deinococcus geothermalis strain and homologous proteins thereof obtained by site mutation, wherein a second-site or fifth-site alanine in a functional domain motif 154LAELAR159 is mutated into serine.Type: ApplicationFiled: May 9, 2016Publication date: June 14, 2018Applicant: BIOTECHNOLOGY RESEARCH INSTITUTE, CHINESE ACADEMY OF AGRICULTURAL SCIENCESInventors: Wei ZHANG, Zhengfu ZHOU, Ming CHEN, Min LIN
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Patent number: 9765313Abstract: The present invention relates to the field of genetic engineering, in particular, the present invention relates to a method for producing a phytase variant with an improved thermal stability, and a phytase variant and the use thereof. The phytase variant contains at least one proline modification, compared to the phytase from Escherichia coli and other mutants thereof. The phytase variants with the modification have preferably improved properties, such as the thermal stability, optimal reaction temperature, pH property, specific activity, protease resistance and performance in animal feeds.Type: GrantFiled: November 12, 2013Date of Patent: September 19, 2017Assignee: Feed Research Institute, Chinese Academy of Agricultural SciencesInventors: Bin Yao, Huoqing Huang, Huiying Luo, Chao Shao, Yingguo Bai, Yaru Wang, Peilong Yang, Pengjun Shi, Kun Meng, Heng Zhao, Rui Ma
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Publication number: 20170240978Abstract: The present invention relates to the field of biotechnology, and in particular to SNP markers as well as use and detection method thereof. The present invention employs whole genome sequencing to conduct whole genome sequencing on a large number of individuals of Apis mellifera sinisxinyuan, and obtains 21 pieces of SNP information specific in Apis mellifera sinisxinyuan, which become marker SNP sites for identifying Apis mellifera sinisxinyuan. The SNP sites provided by the present invention, which are specific in Apis mellifera sinisxinyuan, play important roles in the differentiation of western honey bee in China from the western honey bees in other regions, and in the adaptive evolution of western honey bee in China to the local environment.Type: ApplicationFiled: January 20, 2017Publication date: August 24, 2017Applicant: INSTITUTE OF APICULTURAL RESEARCH, CHINESE ACADEMY OF AGRICULTURAL SCIENCESInventors: Xiao CHEN, Wei SHI, Chao CHEN, Zhiguang LIU, Huihua WANG, Haikun GUO
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Publication number: 20170218461Abstract: The present invention relates to the field of biotechnology, in particular to genes and use thereof. The present invention employs whole genome sequencing to perform whole genome re-sequencing on a large number of individuals of the honey bee Apis mellifera sinisxinyuan, and obtains genes specific to the A. m. sinisxinyuan. The genes play important roles in the differentiation of A. m. sinisxinyuan from the honey bees in other regions and in the adaptive evolution of A. m. sinisxinyuan to the local environment. The Foxo gene or the Ebony gene provided in the present invention can be used to identify A. m. sinisxinyuan from other subspecies; can also be used for studying the genetic diversity of species resources of bees; and can further be used for studying cold resistance genes. This will fill the gap in the research field of A. m. sinisxinyuan by Chinese researchers.Type: ApplicationFiled: January 23, 2017Publication date: August 3, 2017Applicant: INSTITUTE OF APICULTURAL RESEARCH, CHINESE ACADEMY OF AGRICULTURAL SCIENCESInventors: Chao CHEN, Wei SHI, Zhiguang LIU, Xiao CHEN, Huihua WANG, Haikun GUO
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Publication number: 20170218462Abstract: The present invention relates to the field of biotechnology, in particular to genes and use thereof. The present invention employs whole genome sequencing to perform whole genome re-sequencing on a large number of individuals of the honey bee Apis mellifera sinisxinyuan, and obtains genes specific to A. m. sinisxinyuan. The genes play important roles in the differentiation of A. m. sinisxinyuan from the honey bees in other regions and in the adaptive evolution of A. m. sinisxinyuan to the local environment. The FilI gene or the Ds gene provided in the present invention can be used to identify A. m. sinisxinyuan from other subspecies; can also be used for studying the genetic diversity of species resources of bees; and can further be used for studying stress resistance genes. This will fill the gap in the research field of A. m. sinisxinyuan by Chinese researchers.Type: ApplicationFiled: January 23, 2017Publication date: August 3, 2017Applicant: INSTITUTE OF APICULTURAL RESEARCH, CHINESE ACADEMY OF AGRICULTURAL SCIENCESInventors: Zhiguang LIU, Wei SHI, Chao CHEN, Xiao CHEN, Haikun GUO, Huihua WANG
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Publication number: 20170204389Abstract: The present invention relates to the field of genetic engineering and hereditary engineering. The present invention discloses a ?-galactosidase (?-D-galactoside galactohydrolase, EC 3.2.1.23) mutant with high transglycosidase activity, which is obtained by single-site-saturation mutation of amino acid sequences of ?-galactosidase from Aspergillus candidus and Aspergillus oryzae, with own signal peptides removed. The transglycosidase activity of the mutant is over 15% higher than that of wild types. Meanwhile, the present invention also discloses a DNA molecule which encodes the mutant, a recombinant expression vector containing the DNA molecule, and a host cell expressing the DNA molecule. In addition, the present invention also provides a method for preparing ?-galactosidase mutant with high transglycosidase activity by using the recombinant expression vector and applications of the mutant, the DNA molecule, the recombinant expression vector and the host cell in preparation of ?-galactosidase.Type: ApplicationFiled: April 7, 2015Publication date: July 20, 2017Applicant: BIOTECHNOLOGY RESEARCH INSTITUTE, CHINESE ACADEMY OF AGRICULTURAL SCIENCESInventors: Wei ZHANG, Yuhong ZHANG, Bo LIU, Ning SUN, Jialin ZHANG
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Publication number: 20170130241Abstract: A plant type related protein, and a coding gene and an application thereof are provided. The protein is: (a) a protein consisting of the amino acid sequence of SEQ ID NO: 1; (b) a SEQ ID NO: 1-derived protein having substitution, deletion, and/or addition of an amino acid residue on the sequence of SEQ ID NO: 1, and related to the plant type and/or inactivation of a plant brassinolide type, or (c) a protein having more than 80% homology to the sequence of SEQ ID NO: 1 and related to a plant type and inactivation of a plant brassinolide type. The protein and its coding gene have very important value in improving crop production, improving the visual enjoyability of a green plant, implementing simple cultivation of a plant and improving the breeding efficiency, and has a broad prospective in genetic improvement of a plant, new variety cultivation and an application.Type: ApplicationFiled: April 9, 2013Publication date: May 11, 2017Applicant: Cotton Research Institute, Chinese Academy of Agricultural SciencesInventors: Fuguang Li, Zuoren Yang, Chaojun Zhang, Yufen Wang, Zhixia Wu, Chuanliang Liu, Xueyan Zhang, Ye Wang, Fenglian Li, Qianhua Wang, Wengiang Qin, Depei Kong
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Patent number: 9416064Abstract: A method for producing controlled-release fertilizer coated with polyurethane is provided, wherein granular fertilizer is preheated under fluidized state in a fluidized bed; the surface of the granular fertilizer is pretreated after liquid paraffin being atomized; polyol and isocyanate are mixed in a nozzle, then atomized and sprayed onto the surface of the granular fertilizer; after the reaction materials are added, liquid paraffin is added for surface release treatment.Type: GrantFiled: August 6, 2014Date of Patent: August 16, 2016Assignee: CHINESE ACADEMY OF AGRICULTURAL SCIENCES INSTITUTE OF AGRICULTURAL RESOURCES AND REGIONAL PLANNINGInventors: Xiangdong Yang, Bingqiang Zhao, Yanting Li, Juan Li, Zhian Lin, Liang Yuan
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Method for auxiliary identification of inbred line of wuzhishan miniature pig and its special primer
Patent number: 9029090Abstract: A method for auxiliary identification of WZSP inbred line and its special primers are disclosed. The method may comprise: testing whether a deoxyribose nucleotide at site 1273 from 5? end of genomic DNA comprising SEQ ID NO: 1 in a test pig is A or G, measuring whether the test pig genotype is GG, GA or AA wherein the test pig with GG genotype is a candidate for the WZSP inbred line and the test pigs with GA genotype and AA genotype are non-WZSP inbred line. The method can be applied to breed WZSP inbred line, which can be used to preliminarily screen all the pigs in test pig group, eliminate non-WZSP inbred line, find out a candidate WZSP inbred line and make a further confirmation in combination with other methods. The method and primers can also be used to judge whether a WZSP on market is counterfeiting or not.Type: GrantFiled: March 1, 2010Date of Patent: May 12, 2015Assignee: Institute of Animal Science Chinese Academy of Agricultural SciencesInventors: Shutang Feng, Kui Li, Yulian Mu, Shulin Yang -
Patent number: 8742085Abstract: A method for preparing a transgenic animal of simultaneous multiple-gene expression is provided. Additionally, a method for preparing a transgenic embryo, which introduces both phytase gene and human myxovirus resistant gene A into a target embryo, to obtain a transgenic embryo is provided. The transgenic animal of simultaneous multiple-gene expression can be achieved by transplanting the transgenic embryo into the body of a female target animal. A significant advantage of the foregoing methods, among many others, exists in that the simultaneous expression of multiple genes can be achieved in one transgenosis, which provides a convenient method for the preparation of combined-gene transferred animals.Type: GrantFiled: June 24, 2010Date of Patent: June 3, 2014Assignee: Institute of Animal Science, Chinese Academy of Agricultural SciencesInventors: Kui Li, Huiming Ju, Junhua Fan, Lijing Bai, Yulian Mu, Shulin Yang, Zhonglin Tang, Wentao Cui
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Patent number: 8455620Abstract: The present invention relates to a novel phytase enzyme, a novel isolated nucleic acid molecule coding the enzyme, and a novel Yersinia intermedia having phytase activity. Particularly, the present invention relates to the phytase having (a) Theoretical molecular weight 45.5 kDa, (b) high specific activity 3960±248 U/mg, (c) high stability at high temperature and wide pH, (d) optimal pH of 4.0-5.0, (e) optimal temperature of 50-60° C., (f) high resistance to pepsin and trypsin. The phytase is very suitable to be used in feed of monogastrics as feed additive. The present invention also relates to a recombinant vector comprising said nucleic acid molecule, a recombinant host cell (e.g., Pichia pastoris) harboring said recombinant vector, and a method for producing phytase using the recombinant host cell. The present invention further provides a feed additive comprising said phytase and/or host cells expressing a phytase as effective ingredient.Type: GrantFiled: April 30, 2006Date of Patent: June 4, 2013Assignee: Feed Research Institute Chinese Academy of Agricultural SciencesInventors: Bin Yao, Huiying Luo, Huoqing Huang, Yaru Wang, Tiezheng Yuan, Xiuyun Shi, Yingguo Bai, Kun Meng, Peilong Yang
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Publication number: 20130053628Abstract: A method for cultivating a transgenic animal with an increased expression amount of porcine growth hormone is provided. Additionally, a method for cultivating a transgenic embryo, which introduces a recombinant expression vector of 1) TRE-GH or 2) TET-ON into a target embryo to obtain a transgenic embryo with a higher expression amount of porcine growth hormone than a target embryo without introduction is provided. A transgenic animal can be obtained by transplanting the embryo into an animal. The experiments demonstrate that growth hormone (GH) is sharply increased in blood of transgenic pigs after adding DOX.Type: ApplicationFiled: June 24, 2010Publication date: February 28, 2013Applicant: Institute of Animal Science, Chinese Academy of Agricultural SciencesInventors: Kui Li, Huiming Ju, Xinmin Zheng, Lijing Bai, Wentao Cui, Zhonglin Tang, Yulian Mu, Shulin Yang
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METHOD FOR AUXILIARY IDENTIFICATION OF INBRED LINE OF WUZHISHAN MINIATURE PIG AND ITS SPECIAL PRIMER
Publication number: 20120315636Abstract: An auxiliary method for identification of WZSP inbred line and its special primers are provided. The method is to test if DNA at site 1273 from 5? end of genomic DNA SEQ ID NO: 1 in test pig is A or G, measure the test pig genotype is GG, GA or AA; GG or AA genotype is homozygote that DNA at site 1273 is G or A; GA genotype is their heterozygote; test pig of GG genotype is candidate for the WZSP inbred line; test pigs of GA and AA genotypes are not WZSP inbred line. The method can be applied to breed WZSP inbred line, can preliminarily screen all the pigs in test pig group, eliminate non WZSP inbred line, find out candidate WZSP inbred line, combine with other methods for further confirmation. The method can also be used to judge whether WZSP on market is counterfeiting or not.Type: ApplicationFiled: March 1, 2010Publication date: December 13, 2012Applicant: Institute of Animal Science, Chinese Academy of Agricultural SciencesInventors: Shutang Feng, Kui Li, Yulian Mu, Shulin Yang -
Publication number: 20120233717Abstract: A method for preparing a transgenic animal of simultaneous multiple-gene expression is provided. Additionally, a method for preparing a transgenic embryo, which introduces both phytase gene and human myxovirus resistant gene A into a target embryo, to obtain a transgenic embryo is provided. The transgenic animal of simultaneous multiple-gene expression can be achieved by transplanting the transgenic embryo into the body of a female target animal. A significant advantage of the foregoing methods, among many others, exists in that the simultaneous expression of multiple genes can be achieved in one transgenosis, which provides a convenient mean for the preparation of combined-gene transferred animals etc.Type: ApplicationFiled: June 24, 2010Publication date: September 13, 2012Applicant: Institute of Animal Science, Chinese Academy of Agricultural SciencesInventors: Kui Li, Huiming Ju, Junhua Fan, Lijing Bai, Yulian Mu, Shulin Yang, Zhonglin Tang, Wentao Cui
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Patent number: 8207403Abstract: An EPSP synthase (5-enolpyruvylshikimate-3-phosphate synthase) with high glyphosate resistance and a nucleotide sequence encoding the synthase are disclosed. The gene encoding the EPSP synthase has low homology with the reported EPSP synthase. A transgenic plant obtained by the expression of the gene in plant has an increased resistance to glyphosate after experimental confirmation.Type: GrantFiled: November 16, 2007Date of Patent: June 26, 2012Assignee: Biotechnology Research Institute The Chinese Academy of Agricultural SciencesInventors: Min Lin, Aimin Liang, Wei Lu, Liang Li, Ming Chen, Wei Zhang, Shuzhen Ping
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Patent number: 8183220Abstract: A double-effective vaccine vector against foot-and-mouth disease virus having a bicistronic expression vector sequence, the bicistronic expression vector sequence is an antisense gene sequence capable of conjugating with 5? UTR of RNA of the foot-and-mouth disease virus genome and an intact sequence of VP1 structural protein gene of the foot-and-mouth disease virus. Animal experiments show that the vaccine vector provides double effects in terms of gene therapy and gene immunization for the prevention and treatment of foot-and-mouth disease in animals. Also provided are construction methods and methods of use of the vaccine vector.Type: GrantFiled: October 13, 2009Date of Patent: May 22, 2012Assignee: Lanzhou Veterinary Research Institute Chinese Academy of Agricultural SciencesInventors: Jixing Liu, Bin Yang, Xi Lan, Xiangping Yin, Yinmei Bai, Xiaorong Han, Xuerui Li, Baoyu Li, Yuping Fang, Zhiyong Li