Abstract: A gene comprising the nucleic acid sequence of SEQ ID NO:1 and another gene comprising the nucleic acid sequence of SEQ ID NO:2, the latter being artificially synthesized according to plant preferred codons. Both genes encode a protein having the amino acid sequence of SEQ ID NO:3. Also provided are recombinant vectors containing each of the genes and host cells transformed with the recombinant vectors. The host cells can be prokaryotic cells [[and]] or eukaryotic cells. The transgenic plants comprising the gene having the nucleic acid sequence of SEQ ID NO:2 show improved salt and drought tolerance after the [[said]] gene is expressed in the transgenic plants.

Abstract: The present invention belongs to the field of biological detection. Multi-line immunochromatographic test strip for semi-quantitative detection of aflatoxin B1 comprises a paperboard, wherein a water-absorbing pad, a detection pad, a gold-labeled pad and a sample pad are adhered sequentially on one surface of the paperboard from top to bottom, wherein each adjacent pads is overlapped and connected, the detection pad uses a nitrocellulose film as a backing pad, the nitrocellulose film is provided with a transverse control line, a test line I, a test line II and a test line III, wherein the control line is coated with a rabbit anti-mouse polyclonal antibody, and the test line I, test line II and test line III are coated with aflatoxin B1-bovine serum albumin conjugate (AFB1-BSA), respectively: and the gold-labeled pad is transversely coated with a nanogold-labeled anti-aflatoxin B1 monoclonal antibody.

Abstract: DNA molecule for expressing hairpin RNA, the constructing method and the use thereof are provided. The DNA molecule is a closed single-strand cyclic DNA molecule which is constructed by linking four fragments A, B, C and D as following order: A-C-B-D or A-D-B-C, wherein A is a sense strand or anti-sense strand fragment of the target double-strand DNA; B is the fragment which is reversely complementary to A; C and D are DNA fragments with stem-loop structure and they meet one of the following requirements: 1) the nucleotide sequence of fragment C or D comprises at least one sequence e; 2) the nucleotide sequence of fragment C comprises at least one sequence e, the nucleotide sequence of fragment D comprises at least one sequence f, wherein sequences e and f individually are one strand of different restriction endonuclease recognition sequences. The DNA molecule for expressing hairpin RNA and its constructing method are useful in constructing lhRNA library.

Abstract: An EPSP synthase (5-enolpyruvylshikimate-3-phosphate synthase) with high glyphosate resistance and a nucleotide sequence encoding the synthase are disclosed. The gene encoding the EPSP synthase has low homology with the reported EPSP synthase. A transgenic plant obtained by the expression of the gene in plant has an increased resistance to glyphosate after experimental confirmation.

Abstract: Provide a nucleotide coding sequence and another nucleotide coding sequence artificially synthesized according to biased codons of plant. Construct recombinant vectors containing the genes as above and transform them into host cells including prokaryotic cells and eukaryotic cells. It is confirmed that the resulting transgenic plant has improved salt and drought tolerance after the said genes are expressed in the plant.

Abstract: The use of a lignin monomer synthetic gene 4-CL encoding 4-coumarate: CoA ligase from Populus tomentosa in increasing Sclerotinia resistance and lodging resistance. The 4-CL gene is responsible for catalyzing various cinnamic acids and their derivatives to produce respective coenzyme A esters, involved in the synthesis of various downstream secondary products, and is the key gene in the regulation of lignin synthesis. Results of the study show that, increase of lignin content improves plant's resistance against pathogenic infection, enhances the stem strength of rape, and thus significantly improves the lodging resistance. Specifically, the result shows that lignin content of transgenic rape increases more than 10% compared to lignin content of recipient control; lesion extension area of transgenic plant leaves decreases around 30% compared to recipient control; and stem strength of transgenic plant increases up to around 20% compared to control.

Abstract: The invention discloses maize bZIP transcriptional factors, namely, ABP2, ABP4 and ABP9, the genes encoding these factors, and the use thereof. The transcriptional factors are proteins having an amino acid sequence set forth in SEQ ID NO: 2, 4, or 6, or proteins derived therefrom by substitution, deletion or addition of one or more amino acid residues of SEQ ID NO: 2, 4, or 6, and having the same activity as a protein shown by SEQ ID NO: 2, 4, or 6. The ABP2, ABP4 and ABP9 genes encoding these factors, respectively, are the DNA sequences having an identity of more than 90% with a sequence shown by SEQ ID NO: 1, 3 or 5 and the encoded proteins having such same functions. These genes are important for breeding plant varieties with an enhanced tolerance to abiotic stresses and for improving plant tolerance to abiotic stresses.

Abstract: The invention discloses maize bZIP transcriptional factors, namely, ABP2, ABP4 and ABP9, the genes encoding these factors, and the use thereof. The transcriptional factors are proteins having an amino acid sequence set forth in SEQ ID NO: 2, 4, or 6, or proteins derived therefrom by substitution, deletion or addition of one or more amino acid residues of SEQ ID NO: 2, 4, or 6, and having the same activity as a protein shown by SEQ ID NO: 2, 4, or 6. The ABP2, ABP4 and ABP9 genes encoding these factors, respectively, are the DNA sequences having an identity of more than 90% with a sequence shown by SEQ ID NO: 1, 3 or 5 and the encoded proteins having such same functions. These genes are important for breeding plant varieties with an enhanced tolerance to abiotic stresses and for improving plant tolerance to abiotic stresses.

Abstract: The invention discloses maize bZIP transcriptional factors, namely, ABP2, ABP4 and ABP9, the genes encoding these factors, and the use thereof. The transcriptional factors are proteins having an amino acid sequence set forth in SEQ ID NO: 2, 4, or 6, or proteins derived therefrom by substitution, deletion or addition of one or more amino acid residues of SEQ ID NO: 2, 4, or 6, and having the same activity as a protein shown by SEQ ID NO: 2, 4, or 6. The ABP2, ABP4 and ABP9 genes encoding these factors, respectively, are the DNA sequences having an identity of more than 90% with a sequence shown by SEQ ID NO: 1, 3 or 5 and the encoded proteins having such same functions. These genes are important for breeding plant varieties with an enhanced tolerance to abiotic stresses and for improving plant tolerance to abiotic stresses.