Abstract: Match-paired monoclonal antibodies against major royal jelly protein 4 (MRJP4) are secreted by hybridoma cell lines having microbial deposit numbers of CGMCC No. 17294 and CGMCC No. 17295, which are used in an ELISA kit and a colloidal gold immunoassay strip for detecting the MRJP4. The positive and MRJP4-sepcific cell lines are obtained by cell fusion using an antigen of MRJP4 recombinant protein and a cross-reaction with other major royal jelly proteins. The MRJP4 recombinant protein is used as an antigen to obtain several positive and cell lines by cell fusion and twoMRJP4-specific fusion cell lines are obtained by a cross-reaction with other major royal jelly proteins. Primary screening of a matched antibody pair is performed according to antibody pairing for recognizing different epitopes.

Abstract: Provided are a fungus-sourced high-temperature neutral Family-45 cullulase as well as a coding gene and application thereof. The cullulase has optimal pH value of 5.5, and optimal temperature of 60° C., has certain enzyme activity in alkaline condition, and has good alkali resistance, maintains about 70% enzyme activity in optimal condition after being processed at 90° C. for 1 hour, maintains about 50% enzyme activity in optimal condition after being processed in boiling water for 1 hour, and can be well applied in c and other fields.

Abstract: The present invention relates to a method for preparing a whole bovine-derived broadly neutralizing antibody against serotype O foot-and-mouth disease virus, and belongs to the technical field of antibody preparation. The method includes the following steps: 1) conducting the immunization on the cattle; 2) screening for the serotype O foot-and-mouth disease virus antigen-specific single B cells; 3) amplifying variable region genes of the heavy and light chains; 4) acquiring constant region sequences of the heavy and light chains; 5) preparing a full-length heavy chain vector; 6) co-transfecting a cell, taking the supernatant of cell culture, and purifying. The method utilizes different foot-and-mouth disease virus strains to infect cattle and obtains a neutralizing antibody capable of neutralizing three topotypes of serotype O FMDVs by screening, and thus can obtain a whole bovine-derived broadly neutralizing monoclonal antibody.

Abstract: Provided is an amino acid optimization of a functional motif on an IrrE protein of a Deinococcus geothermalis strain and homologous proteins thereof obtained by site mutation, wherein a second-site or fifth-site alanine in a functional domain motif 154LAELAR159 is mutated. into serine.

Abstract: The present invention provides a method for improving transformation efficiency of a plant. The method according to the present invention comprises the use of a nucleic acid encoding the amino acid sequence set forth in SEQ ID NO: 2 or 4, or a nucleic acid encoding a polypeptide comprising an amino acid sequence having at least 85% identity with the amino acid sequence set forth in SEQ ID NO: 2 or 4, and having a function of improving transformation efficiency of a plant.

Abstract: This disclosure relates to the field of molecular biology. Provided are novel genes that encode pesticidal proteins. These pesticidal proteins and the nucleic acid sequences that encode them are useful in preparing pesticidal formulations and in the production of transgenic pest-resistant plants. Methods to create or alter pesticidal proteins are provided for altered or enhanced pesticidal activity.

Abstract: Provided are a glucose oxidase CnGODA, an encoding gene thereof, a recombinant expression vector comprising the gene, and a recombinant strain; the amino acid sequence of the glucose oxidase CnGODA is as represented in SEQ ID NO.1 or SEQ ID NO.2. Further provided is a method for use in preparing glucose oxidase CnGODA, and application of glucose oxidase CnGODA.

Abstract: The present invention provides a flavor extract of low-temperature-pressed peanut cake which is extracted from low-temperature-pressed peanut cake. In the flavor extract, the content of pyrazines flavor substances is not less than 55%, and the content of the aldehydes substances is not more than 15%. The present invention also provides a method for improving the flavor of peanut oil by using the flavor extract of the low-temperature-pressed peanut cake. The flavor extract extracted from peanut cake according to the present invention, which contains 55% or more pyrazines flavor substances, can not only improve the flavor of low-temperature-pressed peanut oil significantly, but also retain the original quality and color of the low-temperature-pressed peanut oil when it is added to low-temperature-pressed peanut oil.

Abstract: The present invention provides an estimate of the prior requested in a crop allocation model. The crop allocation model is a mathematical modelling to estimate crop area, yield and production for 42 major crops in the world across a global 5 arc minute grid. The model uses a downscaling approach that accounts for spatial variation in the biophysical conditions influencing the productivity of individual crops, and uses crop gross revenue potential of alternate crops when considering how to prioritize the allocation of specific crops to individual gridcells. The proposed methodology is an entropy-based optimization procedure that imposes a range of consistency and aggregation constraints. A particular feature of this method is the explicit inclusion of error terms. There is inherent uncertainty in many aspects of the model, such as input data, incomplete information on farmers' behavior, spatial heterogeneity of crop varieties and managements cross regions in the world.

Abstract: The invention belongs to the field of chemical analysis and detection, and specifically relates to a pretreatment method for LC-MS detecting metabolomics of Aspergillus flavus. The method includes: culturing a strain of Aspergillus flavus; quenching the Aspergillus flavus; disrupting the cell membrane of Aspergillus flavus, and extracting a metabolome. The invention adopts a cold glycerol buffer solution combined with a rapid filtration method for quenching, and a MeOH/DCM/ACN/EA/HCOOH mixture is used as an metabolome extract, thereby achieving the object of efficiently extracting different polar compounds, and metabolome compound coverage is high; pretreatment of the cell metabolomics of Aspergillus flavus by the method of the invention can ensure the repeatability and stability of the metabolomics analysis method and reduce the false positive of the test results.

Abstract: Foot-and-mouth disease virus like particles, the particles including a structural protein VP0, a structural protein VP1 including a mineralization peptide, a structural protein VP3, and a calcium phosphate coat. The structural protein VP1 including a mineralization peptide is encoded by a gene sequence represented by SEQ ID NO. 7, SEQ ID NO. 8 or SEQ ID NO. 9. The structural protein VP0 is encoded by a gene sequence represented by SEQ ID NO. 2. The structural protein VP3 is encoded by a gene sequence represented by SEQ ID NO. 3. The calcium phosphate coat covers the structural protein VP0, the structural protein VP1 including a mineralization peptide, and the structural protein VP3.

Abstract: A virus-like particle of Senecavirus A, the particle including a structural protein VP0, a structural protein VP1 and a structural protein VP3. The structural protein VP0 is encoded by a gene sequence represented by SEQ ID NO: 1. The structural protein VP1 is encoded by a gene sequence represented by SEQ ID NO: 2. The structural protein VP3 is encoded by a gene sequence represented by SEQ ID NO: 3.

Abstract: The present disclosure relates to the use of a cpcR regulator gene, which directs the expression of promoters of genes encoding Cry proteins, for reducing the sporulation of a strain of Bacillus thuringiensis, new recombinant strains of Bacillus thuringiensis and uses thereof as biopesticide.

Abstract: A temperature-sensitive attenuated FMDV strain, construction method and applications thereof. The construction method of the temperature-sensitive attenuated FMDV strain is as follows. Mutating a cytosine on K region loop of IRES domain 4 of an FMDV genome to a guanine or an adenine to obtain the temperature-sensitive attenuated FMDV strain, or replacing a K region of IRES domain 4 of an FMDV genome with a K region of IRES domain 4 of a bovine rhinovirus genome to obtain the temperature-sensitive attenuated FMDV strain.

Abstract: High-moisture TPP and its preparation by crushing and mixing low-temperature defatted peanut protein powder and extrusion texturizing at 60° C. to 80° C. in the feeding zone, 90° C. to 100° C. in the mixing zone, 120° C. to 160° C. in the melting zone, 90° C. to 150° C. at the cooling die, and 50° C. to 100° C. in the molding zone. Water is added online during extrusion to adjust the moisture content to 45% to 60%. The cooled product is high-moisture TPP that has a moisture content of 55% or more, bright white color, fragrant taste, abundant fibrous structure, is immediately edible, and can be used as a substitute for meat in the manufacture of chicken dices, pulled meat, vegetarian sausage and the like. The method fully utilizes raw materials, has almost no waste, is continuous, has a high process integration, and low energy consumption, and it can enhance the value of peanut protein powder.

Abstract: The invention relates to a realization method for computer-aided screening of target aptamers and small molecule compounds, which is realized by adopting a molecular docking technology-based reverse virtual screening algorithm and comprises the steps of generating random unrepeated sequences with an appointed length of n based on a sequence length input by a user; modeling a double-stranded DNA structure for each sequence in the random unrepeated sequences and generating a corresponding double-stranded DNA three-dimensional structure file; carrying out format transformation for each generated double-stranded DNA three-dimensional structure file to be used for molecular docking; carrying out format transformation for target small molecules to enable the processed target small molecules to be used for the molecular docking; carrying out molecular docking for each target small molecule and each aptamer; and reading the score files after the molecular docking by two matrix generation functions, and respectively g

Abstract: High-moisture TPP and its preparation by crushing and mixing low-temperature defatted peanut protein powder and extrusion texturizing at 60° C. to 80° C. in the feeding zone, 90° C. to 100° C. in the mixing zone, 120° C. to 160° C. in the melting zone, 90° C. to 150° C. at the cooling die, and 50° C. to 100° C. in the molding zone. Water is added online during extrusion to adjust the moisture content to 45% to 60%. The cooled product is high-moisture TPP that has a moisture content of 55% or more, bright white color, fragrant taste, abundant fibrous structure, is immediately edible, and can be used as a substitute for meat in the manufacture of chicken dices, pulled meat, vegetarian sausage and the like. The method fully utilizes raw materials, has almost no waste, is continuous, has a high process integration, and low energy consumption, and it can enhance the value of peanut protein powder.

Abstract: The present invention provides an estimate of the prior requested in a crop allocation model. The crop allocation model is a mathematical modelling to estimate crop area, yield and production for 42 major crops in the world across a global 5 arc minute grid. The model uses a downscaling approach that accounts for spatial variation in the biophysical conditions influencing the productivity of individual crops, and uses crop gross revenue potential of alternate crops when considering how to prioritize the allocation of specific crops to individual gridcells. The proposed methodology is an entropy-based optimization procedure that imposes a range of consistency and aggregation constraints. A particular feature of this method is the explicit inclusion of error terms. There is inherent uncertainty in many aspects of the model, such as input data, incomplete information on farmers' behavior, spatial heterogeneity of crop varieties and managements cross regions in the world.

Abstract: High-moisture TPP and its preparation by crushing and mixing low-temperature defatted peanut protein powder and extrusion texturizing at 60° C. to 80° C. in the feeding zone, 90° C. to 100° C. in the mixing zone, 120° C. to 160° C. in the melting zone, 90° C. to 150° C. at the cooling die, and 50° C. to 100° C. in the molding zone. Water is added online during extrusion to adjust the moisture content to 45% to 60%. The cooled product is high-moisture TPP that has a moisture content of 55% or more, bright white color, fragrant taste, abundant fibrous structure, is immediately edible, and can be used as a substitute for meat in the manufacture of chicken dices, pulled meat, vegetarian sausage and the like. The method fully utilizes raw materials, has almost no waste, is continuous, has a high process integration, and low energy consumption, and it can enhance the value of peanut protein powder.

Abstract: The present disclosure belongs to the technical field of farm irrigation, and particularly relates to a novel pressureless irrigation device. The novel pressureless irrigation device of the present disclosure uniformly conveys irrigation water to crop plant root systems on the basis of a “connector” principle; and the size of a flow cross-section necessarily satisfies the extremely-low flowing speed requirement of “lossless conveyance” for the “connector” in a static pressure dominant operating state, thereby eliminating a problem of irrigator blockage which accompanies energy dissipation. Water enters the atmosphere via transpiration of crop plants, and a water supply water level control pool at a head portion is triggered to replenish the water. The water consumption and water replenishment are adaptive, and human intervention and additional energy are not required, thereby realizing low-cost and “fool-proof” automatic irrigation and true sense of uniform irrigation.