Abstract: The present disclosure provides a one-pot chemoenzymatic method for site-specific modification and conjugation of antibodies at their Fc glycan site to produce structurally well-defined antibody conjugates carrying defined drugs and other entities. The method is enabled by the discovery that certain endoglycosidases have the ability to both deglycosylate an antibody and to recognize selectively modified small disaccharide oxazolines for transglycosylation on antibodies without hydrolysis of the resulting products.

Abstract: The present disclosure provides a preparation method of AM-type polystyrene microsphere ofloxacin imprinted polymer as well as an application thereof. A monomer acrylamide and an initiator ammonium persulfate are subjected to graft polymerization on the surface of modified polystyrene primary amine resin, to get grafted particles; then an adsorption test of a levofloxacin solution by the grafted particles PAM/PSA is conducted, and then a levofloxacin surface molecularly imprinted material MIP-PAM/PSA is prepared by using ethylene glycol diglycidyl ether as the crosslinking agent. The present disclosure can realize the separation and purification of racemic ofloxacin effectively, thus providing a new method and material for separating and enriching s-type ofloxacin in the industry. Because the antibacterial efficacy of S-ofloxacin on Gram-negative bacteria and positive bacteria is 8-128 times that of its enantiomer R-ofloxacin, so the present technology can improve the efficacy of a drug greatly.

Abstract: Disclosed herein are universal donor stem cells and related methods of their use and production. The universal donor stem cells disclosed herein are useful for overcoming the immune rejection in cell-based transplantation therapies. In certain embodiments, the universal donor stem cells disclosed herein do not express one or more MHC-I and MHC-II human leukocyte antigens. Similarly, in certain embodiments, the universal donor stem cells disclosed herein do not express one or more human leukocyte antigens (e.g., HLA-A, HLA-B and/or HLA-C) corresponding to MHC-I and MHC-II human leukocyte antigens, thereby rendering such cells hypoimmunogenic.

Abstract: A multimodal imaging means for evaluating tissue oxygenation status, its working method and application are provided. The means includes: a test object acquisition module, a basic data measurement module, a grayscale ultrasound module, a photoacoustic imaging module, a planar wave imaging module, a calculation module, and a statistical analysis module. Structural information, oxygenation status data, and vascular density information of placenta of pregnant rats are obtained, diagnostic ability AUC of receiver operating characteristics ROC in a diagnostic mode is calculated with a standard method, and statistical analysis is conducted. It can evaluate tissue oxygen saturation in real-time and non-invasive manner, and an evaluation result is more accurate.

Abstract: The present invention generally relates to nanowires. In one aspect, the present invention is generally directed to systems and methods of individually addressing nanowires on a surface, e.g., that are substantially upstanding or vertically-oriented with respect to the surface. In some cases, one or more nanowires may be individually addressed using various integrated circuit (“IC”) technologies, such as CMOS. For example, the nanowires may form an array on top of an active CMOs integrated circuit.

Abstract: This disclosure describes a novel “kick and kill” strategy as an effective cancer therapy for treating virus-associated cancers. In particular, this disclosure provides a method of reactivating a latent Epstein-Barr virus (EBV) in a cell infected with the EBV. Also provided are a method of eliciting or enhancing an immune response against an EBV-positive cancer cell in a subject infected with the EBV and a method of treating a subject having cancer associated with EBV infection.

Abstract: Compositions and methods are provided herein for conducting prime editing of a target DNA molecule (e.g., a genome) that enables the incorporation of a nucleotide change and/or targeted mutagenesis. The compositions include fusion proteins comprising nucleic acid programmable DNA binding proteins (napDNAbp) and a polymerase (e.g., reverse transcriptase), which is guided to a specific DNA sequence by a modified guide RNA, named an PEgRNA. The PEgRNA has been altered (relative to a standard guide RNA) to comprise an extended portion that provides a DNA synthesis template sequence which encodes a single strand DNA flap which is synthesized by the polymerase of the fusion protein and which becomes incorporated into the target DNA molecule.

Abstract: Embodiments of the disclosure include methods and compositions for producing NKT cells effective for immunotherapy and also methods and compositions for providing an effective amount of NKT cells to an individual in need of immunotherapy. In specific embodiments, the NKT cells are CD62L+ and have been exposed to one or more costimulatory agents to maintain CD62L expression. The NKT cells may be modified to incorporate a chimeric antigen receptor, in some cases.

Abstract: Embodiments of the disclosure include methods and compositions in which adoptive T cell therapy is generated by particular methods in which a population of the T cells are directed against one or more neoantigens of any kind. In specific embodiments, the T cells are produced following exposure of PBMCs to peptides that encompass one or more antigens, and the produced T cells are tested for their efficacy. Following identification of efficacious peptides, they are utilized to produce the adoptive T cell therapy against the neoantigen(s).

Abstract: The disclosure relates to a valve (20) for use with breathing assistance apparatus.

Abstract: The invention discloses a drop test device for a case of a vehicle battery pack. The device includes a bracket body, and a mounting frame is suspended horizontally on an upper end surface of the bracket body. The two mounting frames are fixed parallel to the bracket body, and a fixing frame is horizontally fixed below each of the mounting frames on the bracket body. A sliding seat is slidably arranged between the two mounting frames, and one side of the fixing frame is provided with a side connecting plate. A sliding rail is arranged between the sliding seat and the side connecting plate, and a simulated platform mechanism is slidably arranged on the sliding rail for body installation of the vehicle battery pack so as to design installation states of the vehicle battery pack according to different models.

Abstract: Methods, systems, compositions and strategies for the delivery of WW domain-containing fusion proteins into cells in vivo, ex vivo, or in vitro via ARMMs are provided. Methods, systems, compositions and strategies for the delivery of Cas9 proteins and/or Cas9 variants into cells in vivo, ex vivo, or in vitro via fusion to ARMM associated proteins (e.g., ARRDC1 or TSG101) are also provided.

Abstract: This disclosure addresses burdensome pulmonary aspirations and proposes a non-invasive system and device capable of detecting gastric distension as a predictive “warning sign” of aspiration risk and a treatable risk factor to reduce the risk of highly morbid and often lethal aspiration events.

Abstract: Described herein are antibodies or variants thereof that specifically bind to coronavirus antigens, such as SARS-CoV-2 antigens. The antibodies can be neutralizing antibodies. Also provided are methods of using the antibodies, including methods of treating a subject infected with SARS-CoV-2, and methods of diagnosing a subject infected with SARS-CoV-2.

Abstract: The present invention generally relates to systems and methods for imaging or determining nucleic acids, for instance, within cells. In some embodiments, the transcriptome of a cell may be determined. Certain embodiments are directed to determining nucleic acids, such as mRNA, within cells at relatively high resolutions. In some embodiments, a plurality of nucleic acid probes may be applied to a sample, and their binding within the sample determined, e.g., using fluorescence, to determine locations of the nucleic acid probes within the sample. In some embodiments, codewords may be based on the binding of the plurality of nucleic acid probes, and in some cases, the codewords may define an error-correcting code to reduce or prevent misidentification of the nucleic acids. In certain cases, a relatively large number of different targets may be identified using a relatively small number of labels, e.g., by using various combinatorial approaches.

Abstract: Embodiments of the disclosure include methods and compositions for enhancing expansion of immune cells for immunotherapy. In particular embodiments, immune cells, such as T-cells, express a constitutively active cytokine receptor in which the transmembrane and endodomains are able to provide an activating signal separately from any input to the corresponding exodomain to which they are operably linked. In specific embodiments, the transmembrane and endodomain from IL-7R? is utilized with the exodomain of CD34.

Abstract: A composite material is disclosed including: a first material including a plurality of crosslinked first polymer chains including a plurality of first polymer monomeric units; a coating layer on the surface of the first material, wherein the coating layer includes a plurality of adhesion polymer chains, wherein the plurality of adhesion polymer chains includes a plurality of the first polymer monomeric units and a plurality of first bond-forming units, wherein the adhesion polymer chains are interwoven with the first polymer chains; and a second material including a plurality of second polymer chains, wherein the coating layer is disposed in-between the first and the second material and contacting the surface of the first and the second material, and a portion of the second polymer chains includes a plurality of second polymer monomeric units and second bond-forming units; wherein the first and the second bond-forming units form one or more bonds.

Abstract: A quantum computer uses interactions between atomic ensembles mediated by an optical cavity mode to perform quantum computations and simulations. Using the cavity mode as a bus enables all-to-all coupling and execution of non-local gates between any pair of qubits. Encoding logical qubits as collective excitations in ensembles of atoms enhances the coupling to the cavity mode and reduces the experimental difficulty of initial trap loading. By using dark-state transfers via the cavity mode to enact gates between pairs of qubits, the gates become insensitive to the number of atoms within each collective excitation, making it possible to prepare an array of qubits through Poissonian loading without feedback.

Abstract: Devices and methods are disclosed for remote clinical monitoring performance of exercises using a smart resistance exercise device including a resistance band, a first handle connected to a first end of the resistance band and a second handle connected to a second end of the resistance band, a force sensing assembly operably coupled to the resistance band, and a local receiving device communicatively coupled to the force sensing assembly. The force sensing assembly of the device includes a housing, and a force sensor disposed in the housing and operatively connected to the resistance band to measure a force exerted on the resistance band. The force sensing assembly also includes a processing and communication module communicatively coupled to the force sensor to receive measurements of the force sensor and communicatively coupled to the local receiving device to transmit the measurements to the local receiving device.

Abstract: Compositions are described for direct protein delivery into multiple cell types in the mammalian inner ear. The compositions are used to deliver protein(s) (such as gene editing factors) editing of genetic mutations associated with deafness or associated disorders thereof. The delivery of genome editing proteins for gene editing and correction of genetic mutations protect or restore hearing from genetic deafness. Methods of treatment include the intracellular delivery of these molecules to a specific therapeutic target.