Abstract: Embodiments of the disclosure include high-throughput profiling of transcriptomes of subcellular compartments or structures, including a droplet-based single-cell total-RNA-seq method that enables profiling of transcripts localized in particular subcellular compartment or structures. In specific embodiments, the disclosure provides for transcriptome profiling of single nuclei that allows for construction of a cell atlas using only long non-coding RNA species that can be applied for tissue-wide identification of cell-type-specific lncRNA species.
Abstract: Novel small molecule proteolysis-targeting chimeras (PROTACs) are provided, along with methods for their use as Bruton's tyrosine kinase (BTK) degraders. The small molecule PROTACs described herein are useful in treating and/or preventing BTK-related diseases, such as cancer, neurodegenerative disorders, inflammatory diseases, and metabolic disorders. Also provided are methods for inducing BTK degradation in a cell using the compounds and compositions described herein.
Type:
Application
Filed:
May 15, 2024
Publication date:
November 21, 2024
Applicant:
Baylor College of Medicine
Inventors:
Wen-Hao Guo, Xin Yu, Ran Cheng, Jin Wang
Abstract: The present invention is based, in part, on the discovery of the human-specific regulatory control of cGAS and the structure of the active human cGAS-DNA complex, as well as compositions comprising the modified hcGAS polypeptide, hcGAS-DNA complex, hcGAS-DNA-ATP complex, and methods of screening for modulators of the structure, expression, and/or activity of such polypeptides and complexes.
Type:
Grant
Filed:
June 26, 2019
Date of Patent:
November 19, 2024
Assignees:
President and Fellows of Harvard College, Dana-Farber Cancer Institute, Inc.
Inventors:
Philip J. Kranzusch, John J. Mekalanos, Aaron T. Whiteley, Wen Zhou
Abstract: Disclosed is a neural computer including an image sensor capable of controlling a photocurrent. The neural computer according to an embodiment includes a preprocessor configured to receive an image and generate a feature map for the received image; a flattening unit configured to transform the feature map generated by the preprocessor into tabular data to provide data output; and an image classifier configured to classify images received through the preprocessor by using the data output by the flattening unit as an input value. The preprocessor includes an optical signal processor configured to receive the image and generate the feature map.
Type:
Grant
Filed:
October 13, 2021
Date of Patent:
November 19, 2024
Assignees:
Samsung Electronics Co., Ltd., President and Fellows of Harvard College
Inventors:
Changhyun Kim, Houk Jang, Henry Julian Hinton, Hyeonjin Shin, Minhyun Lee, Donhee Ham
Abstract: N-type polymer based electrochemical devices include one or more source electrodes, one or more drain electrodes, one or more channels, a gate electrode, and an electrolyte solution are disclosed. The channels include one or more n-type polymers and one or more enzymes. The gate electrode includes one or more n-type polymers and one or more enzymes. The source and the drain electrodes are electrically connected by the corresponding channel. The electrolyte solution contains one or more metabolites capable of reacting with the one or more enzymes in the channel and the gate electrode and is in electrical contact with the channel and the gate electrode. Saturation current that flows through the channel increases when the metabolites react with the enzymes to produce electrons, which are directly transferred to the n-type polymers at the gate electrode and the channel. Methods of making and using the n-type electrochemical device are also disclosed.
Type:
Grant
Filed:
January 18, 2019
Date of Patent:
November 19, 2024
Assignees:
King Abdullah University of Science and Technology, Institut Mines Telecom, Imperial College London
Inventors:
Sahika Inal, Anna-Maria Pappa, Alexander Giovannitti
Abstract: A microfluidic device includes a first layer of a porous material with pores having a first average pore size and a liquid-receiving area through which a liquid sample is received into the microfluidic device. A second layer of another porous material, with pores of a second average pore size, is stacked below the first layer and has a channel with a starting end positioned at least in part in an overlapping manner with the liquid-receiving area. The channel has a terminating end extending laterally at a predetermined wicking distance from the starting end. The first average pore size and the second average pore size cause a wicking effect in which at least some of the liquid sample flows along the channel at least a portion of the wicking distance between the starting end and the terminating end.
Type:
Grant
Filed:
September 18, 2023
Date of Patent:
November 19, 2024
Assignee:
Trustees of Tufts College
Inventors:
Charles R. Mace, Syrena C. Fernandes, Samuel B. Berry
Abstract: An apparatus and method for medical imaging uses a first and second contrast agent, the second agent targeted to a particular tissue type. First images are obtained using the first agent, and second images using the second agent using medical imaging systems. An image processing system is adapted to process the first and second medical images by fitting parameters of a pharmacokinetic model to the first medical images, identifying a nontargeted tissue type, scaling the fitted parameters to best match the nontargeted tissue in the second medical images, executing the pharmacokinetic model to prepare a correction image, and generating corrected medical images by subtracting the correction image from the second medical images.
Type:
Grant
Filed:
November 13, 2023
Date of Patent:
November 19, 2024
Assignee:
The Trustees of Dartmouth College
Inventors:
Kenneth M. Tichauer, Scott C. Davis, Brian William Pogue
Abstract: In some aspects, a device comprises a plurality of walls defining a slot and one or more channels wherein the one or more channels are intersected by the slot; wherein the one or more channels have a cross-section in a plane perpendicular to a vertical axis of the device that changes along the vertical axis; and one or more floats sized to allow movement of the one or more floats within said one or more channels, wherein the one or more floats have a surface characteristic that is different from the surface characteristic of the walls such that, upon contact with a fluid, said walls and said floats form different contact angles and induce a repulsive capillary force between the walls and the one or more floats at a surface of the fluid; and wherein the slot has a width that is smaller than the width of the one or more channels such that the one or more floats are prevented from moving between the one or more channels.
Type:
Grant
Filed:
August 28, 2020
Date of Patent:
November 19, 2024
Assignee:
President and Fellows of Harvard College
Inventors:
Maya Faaborg, Cheng Zeng, Michael P. Brenner, Yohai Bar-Sinai, Ahmed Sherif, Vinothan N. Manoharan
Abstract: Fabrication of a low-cost and miniaturized solid-contact thread-based ion sensing electrode (ISE) that is suitable for point-of-care applications is described. The fabrication process is simple and scalable. Different types of threads and a variety of different conductive ion-to-electron transducer inks can be used to fabricate the thread-based ISEs with Nernstian responses. Multiple thread-based sensors can be easily bundled with the reference electrode to fabricate a customized sensor for multiplexed ion-sensing in small sample volumes. The thread-based electrodes can be used in both single-use and reusable fashion. The thread-based multiplexed ion-sensor is used for detection of a wide array of ions in many different types of aqueous solutions.
Type:
Grant
Filed:
November 13, 2018
Date of Patent:
November 19, 2024
Assignee:
President and Fellows of Harvard College
Inventors:
Seyedeh Moloud Mousavi, George M. Whitesides
Abstract: Systems and methods for focused direction deposition of a micron or nanometer dimension polymeric fiber and materials of such fibers are described herein. Systems and methods employ one or more gas flows to entrain and deflect fibers produced by a rotary jet spinning system forming a focused fiber stream. Some embodiments enable control of alignment and distribution of the fibers with a relatively high fiber throughput.
Type:
Grant
Filed:
January 14, 2020
Date of Patent:
November 12, 2024
Assignee:
President and Fellows of Harvard College
Inventors:
Qihan Liu, Kevin Kit Parker, Huibin Chang
Abstract: A textile actuator and harness system can include a harness configured to be worn with a portion extending across a wearer's joint. The harness comprises a substantially inextensible section and at least two mounting locations spaced for positioning across the joint, with at least one located along the substantially inextensible section of the harness. A textile envelope defines a chamber and is made fluid-impermeable by (a) a fluid-impermeable bladder contained in the textile envelope and/or (b) a fluid-impermeable structure incorporated into the textile envelope. The textile envelope is secured to the harness at each mounting location, and the textile envelope has a pre-determined geometry configured to produce assistance to the joint due to inflation of the textile envelope during a relative increase in pressure inside the chamber.
Type:
Grant
Filed:
December 4, 2019
Date of Patent:
November 12, 2024
Assignee:
President and Fellows of Harvard College
Inventors:
Conor J. Walsh, Ciaran O'Neill, Nathan Phipps
Abstract: A nanopore system provided herein includes first and second fluidic reservoirs in fluidic communication with a nanopore forming a fluidic path between the reservoirs. An enzyme clamp, provided in the first fluidic reservoir, abuts the nanopore and is reversibly bound to a sequential plurality of polymer subunits of a target polymer molecule in ionic solution. The clamp has an outer clamp diameter that is greater than the nanopore diameter. An electrical circuit includes an electrode in each of the reservoirs for applying a voltage bias across the nanopore. A pulse generator is connected in the electrical circuit to apply control pulses across the nanopore to step the clamp along sequential polymer subunits of the target polymer molecule. The system includes no fuel or source of fuel for the clamp. A controller is connected in the electrical circuit for controlling the collection of electrical indications of polymer subunits.
Type:
Grant
Filed:
May 13, 2021
Date of Patent:
November 12, 2024
Assignee:
President and Fellows of Harvard College
Inventors:
Daniel Branton, Stephen Jordan Fleming, Jene A. Golovchenko
Abstract: The disclosure describes various aspects related to enabling effective multi-qubit operations, and more specifically, to techniques for enabling parallel multi-qubit operations on a universal ion trap quantum computer. In an aspect, a method of performing quantum operations in an ion trap quantum computer or trapped-ion quantum system includes implementing at least two parallel gates of a quantum circuit, each of the at least two parallel gates is a multi-qubit gate, each of the at least two parallel gates is implemented using a different set of ions of a plurality of ions in a ion trap, and the plurality of ions includes four or more ions. The method further includes simultaneously performing operations on the at least two parallel gates as part of the quantum operations. A trapped-ion quantum system and a computer-readable storage medium corresponding to the method described above are also disclosed.
Type:
Grant
Filed:
April 28, 2023
Date of Patent:
November 12, 2024
Assignees:
IonQ, Inc., University of Maryland, College Park
Inventors:
Caroline Figgatt, Aaron Ostrander, Norbert M. Linke, Kevin A. Landsman, Daiwei Zhu, Dmitri Maslov, Christopher Monroe
Abstract: The disclosure provides rechargeable lithium ion batteries comprising at least one lithium salt-graphite composite electrode. In particular, the disclosure provides a rechargeable “water-in-bisalt” lithium ion battery with a high potential where at least a portion of the lithium salt is phase separated from the aqueous electrolyte, and where the anionic-redox reaction occurs within the graphitic lattice.
Abstract: An afocal sensor assembly detects a light beam with an aberrated wavefront. The afocal sensor assembly is configured to provide sorted four-dimensional (4D) light field information regarding the light beam, for example, via one or more plenoptic images. Based on the 4D light field information, a lossy reconstruction of an aberrated wavefront for one or more actuators of an adaptive optics (AO) device is performed. The AO device can be controlled based on the lossy reconstruction to correct the wavefront of the light beam. In some embodiments, the aberrated wavefront is due to passage of the light beam through atmospheric turbulence, and the lossy reconstruction and correction using the AO device is performed in less than 1.0 ms. The lossy reconstruction of the aberrated wavefront can have a phase accuracy in a range of ?/2 to ?/30.
Type:
Grant
Filed:
December 2, 2021
Date of Patent:
November 12, 2024
Assignee:
University of Maryland, College Park
Inventors:
Chensheng Wu, Jonathan Ko, John R. Rzasa, Christopher C. Davis, Daniel Paulson
Abstract: The present invention provides methods for preparing an alpha-keratin solution comprising alpha-keratin protofibrils and intermediate filaments in liquid crystal phase and uses thereof for fabricating, e.g., shape-memory polymeric fibers, yarns, threads, fabrics, structures and objects.
Type:
Grant
Filed:
July 26, 2019
Date of Patent:
November 12, 2024
Assignee:
President and Fellows of Harvard College
Abstract: The disclosure provides compounds that inhibit the invasion of host cells by intracellular parasites. These find use, for example, in treating and preventing periodontitis or a periodontitis-related condition or symptom.
Type:
Application
Filed:
July 16, 2024
Publication date:
November 7, 2024
Applicants:
Meharry Medical College, Duke University
Abstract: The present disclosure provides compounds of Formula (I) and (II), which may be ROCK2 inhibitors. The present disclosure also provides pharmaceutical compositions and kits comprising the compounds, and methods of treating or preventing diseases and disorders associated with ROCK2 (e.g., fibrotic disease, autoimmune disease, inflammatory-fibrotic condition, inflammatory condition, edema, ophthalmic disease, cardiovascular disease, central nervous system disorder, cancer) by administering to a subject in need thereof the compounds or pharmaceutical compositions.
Type:
Application
Filed:
July 26, 2022
Publication date:
November 7, 2024
Applicants:
President and Fellows of Harvard College, Trustees of Boston University
Abstract: The approach disclosed herein is a process for non-equilibrium chemical and materials processing using the combination of non-equilibrium plasma, non-equilibrium multi-functional catalysis, a precisely programed heating and quenching (PHQ), and supersonic reaction quenching to dynamically change the chemical equilibrium and increase the yield and selectivity of the products. An important feature of the disclosed approach is to realize an efficient and high selectivity synthesis method of chemicals and materials by using non-chemical equilibrium, non-equilibrium catalysts, and non-equilibrium of excited states via active control of molecule excitation by low temperature hybrid plasma, dynamics of chemical reactions by programed heating and supersonic quenching, and the design of non-equilibrium catalysts by thermal shocks and plasma coupling to enable distributed and electrified chemical synthesis of hydrogen, ammonia, valued carbon and other chemical products at atmospheric conditions.
Type:
Application
Filed:
March 31, 2022
Publication date:
November 7, 2024
Applicants:
The Trustees of Princeton University, University of Maryland, College Park