Abstract: The present invention relates to the production of steviol glycoside rebaudiosides D4, WB1 and WB2 and the production of rebaudioside M from Reb D4.
Type:
Grant
Filed:
August 30, 2018
Date of Patent:
August 24, 2021
Assignee:
Conagen Inc.
Inventors:
Guohong Mao, Jacob Edward Vick, Michael Batten, Yang Luo, Yilin Wu, Beihua Zhang, David Byun, Xiaodan Yu
Abstract: The invention provides a recombinant Thraustochytriaceae cell for the production of a glycomolecule. The cell comprises a nucleic acid encoding a heterologous glycomolecule, and a sequence encoding a heterologous oligosaccharyltransferase. The cell produces the heterologous glycomolecule having a higher glycan occupancy compared to the same heterologous glycomolecule produced by a corresponding cell not comprising the heterologous oligosaccharyltransferase. The glycan occupancy can be more than 25%. The cells advantageously produce and, optionally secrete, the heterologous glycomolecule. Thus, the invention provides recombinant organisms that provide glycomolecules having a glycosylation profile that is more similar to the glycosylation profile produced in a manunalian cell.
Type:
Application
Filed:
October 30, 2020
Publication date:
June 24, 2021
Applicant:
Conagen Inc.
Inventors:
Nicky C. Caiazza, Jun Urano, Spiros Kambourakis
Abstract: The present disclosure relates to the production of steviol glycosides rebaudioside J and rebaudioside N through the use of rebaudioside A as a substrate and a biosynthetic pathway involving various 1,2 RhaT-rhamnosyltransferases.
Type:
Application
Filed:
December 2, 2020
Publication date:
June 24, 2021
Applicant:
Conagen Inc.
Inventors:
Guohong Mao, Michael Batten, Phillip Hunt, Oliver (Xiaodan) Yu
Abstract: Disclosed is a steviol glycoside referred to as rebaudioside D3. Rebaudioside D3 has five ?-D-glucosyl units connected to the aglycone steviol. Also disclosed are methods for producing rebaudioside D3, a UDP-glycosyltransferase fusion enzyme, and methods for producing rebaudioside D and rebaudioside E.
Type:
Grant
Filed:
April 10, 2020
Date of Patent:
March 9, 2021
Assignee:
Conagen Inc.
Inventors:
Guohong Mao, Venkata Sai Prakash Chaturvedula, Xiaodan Yu
Abstract: The present invention relates to the production of steviol glycoside rebaudiosides D4, WB1 and WB2 and the production of rebaudioside M from Reb D4.
Type:
Application
Filed:
June 18, 2020
Publication date:
January 7, 2021
Applicant:
Conagen Inc.
Inventors:
Guohong Mao, Jacob Edward Vick, Michael Batten, David Byun, Yang Luo, Yilin Wu, Beihua Zhang, Xiaodan Yu
Abstract: The present disclosure relates to the production of steviol glycosides rebaudioside J and rebaudioside N through the use of rebaudioside A as a substrate and a biosynthetic pathway involving various 1,2 RhaT-rhamnosyltransferases.
Type:
Grant
Filed:
November 8, 2019
Date of Patent:
January 5, 2021
Assignee:
Conagen Inc.
Inventors:
Guohong Mao, Michael Batten, Phillip Hunt, Oliver (Xiaodan) Yu
Abstract: The invention provides recombinant organisms containing a nucleic acid encoding a heterologous glycomolecule that has a low sulfation profile or that is unsulfated. In one embodiment the heterologous glycomolecule is an immunoglobulin molecule. The recombinant organisms have a genetic modification to at least one sulfotransferase gene, such as a deletion, disruption, or other genetic modification. The cells advantageously produce and, optionally secrete, the heterologous glycomolecule. Thus, the invention provides recombinant organisms that provide glycomolecules having a glycosylation profile that is more similar to the glycosylation profile produced in a mammalian cell, and therefore may be safer and more effective for use as a therapeutic in humans or animals. The glycomolecules can be a glycoprotein, glycopeptide, or a glycolipid.
Abstract: Disclosed is a steviol glycoside referred to as rebaudioside D3. Rebaudioside D3 has five ?-D-glucosyl units connected to the aglycone steviol. Also disclosed are methods for producing rebaudioside D3, a UDP-glycosyltransferase fusion enzyme, and methods for producing rebaudioside D and rebaudioside E.
Type:
Application
Filed:
April 10, 2020
Publication date:
November 19, 2020
Applicant:
Conagen Inc.
Inventors:
Guohong Mao, Venkata Sai Prakash Chaturvedula, Xiaodan Yu
Abstract: Disclosed are steviol glycosides referred to as rebaudioside V and rebaudioside W. Also disclosed are methods for producing rebaudioside M (Reb M), rebausoside G (Reb G), rebaudioside KA (Reb KA), rebaudioside V (Reb V) and rebaudioside (Reb W).
Abstract: Disclosed are steviol glycosides referred to as rebaudioside V and rebaudioside W. Also disclosed are methods for producing rebaudioside M (Reb M), rebausoside G (Reb G), rebaudioside KA (Reb KA), rebaudioside V (Reb V) and rebaudioside (Reb W).
Abstract: Disclosed are steviol glycosides referred to as rebaudioside V and rebaudioside W. Also disclosed are methods for producing rebaudioside M (Reb M), rebausoside G (Reb G), rebaudioside KA (Reb KA), rebaudioside V (Reb V) and rebaudioside (Reb W).
Abstract: The present disclosure generally relates to novel polynucleotide molecules for use in regulating gene expression in recombinant cells, such as labyrinthulomycetes cells. The disclosure further relates to nucleic acid constructs, such as vectors and expression cassettes, containing a regulatory element operably linked to a heterologous nucleotide sequence. The disclosure further relates to methods for stably transforming a host cell, such as a labyrinthulomycetes cell with transgenes. Stably transformed recombinant cells, progeny, biomaterials derived therefrom, and methods for preparing and using the same are also provided.
Type:
Application
Filed:
March 16, 2020
Publication date:
October 8, 2020
Applicant:
Conagen Inc.
Inventors:
Nicky C. Caiazza, Maung N. Win, Jun Urano
Abstract: The present disclosure relates to the production of 8-methyl nonanoic acid and medium chain branched fatty acids, e.g., via microbial fermentation.
Abstract: The present disclosure relates to the use of beta-glucosidase to enhance the production efficiency of desired steviol glycosides, such as rebaudioside M (reb M).
Type:
Application
Filed:
December 23, 2019
Publication date:
September 17, 2020
Applicant:
Conagen Inc.
Inventors:
Guohong Mao, Jacob Edward Vick, Michael Batten, Oliver Yu
Abstract: Disclosed are steviol glycosides referred to as rebaudioside V and rebaudioside W. Also disclosed are methods for producing rebaudioside M (Reb M), rebausoside G (Reb G), rebaudioside KA (Reb KA), rebaudioside V (Reb V) and rebaudioside (Reb W).
Abstract: Disclosed are whole-cell catalysts, methods of making the whole-cell catalysts, and methods of using the whole-cell catalysts to produce steviol glycosides.
Type:
Grant
Filed:
April 14, 2016
Date of Patent:
August 25, 2020
Assignee:
Conagen Inc.
Inventors:
Guohong Mao, Jacob Edward Vick, Shi-Yan Li, Xiaodan Yu
Abstract: The present invention relates to the production of steviol glycoside rebaudiosides D4, WB1 and WB2 and the production of rebaudioside M from Reb D4.
Type:
Grant
Filed:
April 12, 2019
Date of Patent:
July 28, 2020
Assignee:
Conagen Inc.
Inventors:
Guohong Mao, Jacob Edward Vick, Michael Batten, David Byun, Yang Luo, Yilin Wu, Beihua Zhang, Xiaodan Yu
Abstract: Disclosed are methods for ergothioneine biosynthesis. More particularly, the present disclosure relates to methods for microbial ergothioneine biosynthesis.