Patents Assigned to Dade Behring Marburg GmbH
  • Patent number: 6414116
    Abstract: The present invention relates to immunoreactive peptides that are homologous with the region encompassing amino acid positions 450 to 655 of glycoprotein II of varicella zoster virus. In this context, preference is given to those peptides corresponding to segments of amino acids 505 to 647, 517 to 597, 535 to 584 or 545 to 582. The immunoreactive peptides are useful for methods of diagnosing varicella zoster virus infection.
    Type: Grant
    Filed: December 23, 1998
    Date of Patent: July 2, 2002
    Assignee: Dade Behring Marburg GmbH
    Inventors: Markus Eickmann, Dorothee Gicklhorn, Klaus Radsak, Hans-Peter Hauser, Bernhard Giesendorf
  • Patent number: 6410696
    Abstract: A method of measuring the amount of cyclosporin in a sample suspected of containing cyclosporin is disclosed. A method of inactivating interfering cross-reactive material in an assay for measuring the amount of cyclosporin in a sample suspected of containing cyclosporin is also disclosed. Compositions wherein cyclosporin is conjugated to an immunogenic carrier or a label, optionally through a linking group, at an alanine nitrogen atom of the cyclic backbone of cyclosporin are also disclosed. Compositions wherein atiocyclosporin is conjugated, optionally through a linking group, to an immunogenic carrier or a label are also disclosed. Where cyclosporin is conjugated to an immunogenic carrier, the conjugates may be used as immunogens for the preparation of antibodies which are capable of recognizing cyclosporin.
    Type: Grant
    Filed: November 13, 2000
    Date of Patent: June 25, 2002
    Assignee: Dade Behring Marburg GmbH
    Inventors: Dariush Davalian, Maureen H. Beresini, Svetlana Alexander, Mae Wan-Leng Hu, Edwin F. Ullman
  • Patent number: 6406667
    Abstract: Methods, compositions and kits are disclosed. The methods are directed to determining the presence or relative amounts of two or more components in a medium. A combination is provided comprising a medium suspected of containing the components and a label reagent for each of the components. The label reagent comprises a chemiluminescent composition that is activated by electromagnetic radiation. A first specific binding pair (sbp) member may be associated with the reagent depending on the components to be determined. Luminescence emitted by each of the chemiluminescent compositions upon activation is differentially detectable. Where a first sbp member is employed, it is capable of binding to the component or to a second sbp member to form a complex related to the amount of the component. At least one of the chemiluminescent compositions comprises a fluorescent energy acceptor. After the above are combined, the chemiluminescent compositions are activated.
    Type: Grant
    Filed: June 15, 1999
    Date of Patent: June 18, 2002
    Assignee: Dade Behring Marburg GmbH
    Inventors: Sharat Singh, Edwin F. Ullman
  • Patent number: 6406913
    Abstract: Methods are disclosed for determining an analyte in a medium suspected of containing the analyte. One method comprises treating a medium suspected of containing an analyte under conditions such that the analyte, if present, causes a photosensitizer and a chemiluminescent compound to come into close proximity. The photosensitizer generates singlet oxygen and activates the chemiluminescent compound when it is in close proximity. The activated chemiluminescent compound subsequently produces light. The amount of light produced is related to the amount of analyte in the medium. Preferably, at least one of the photosensitizer and chemiluminescent compound is associated with a surface which is usually a suspendible particle, and a specific binding pair member is bound thereto. Compositions and kits are also disclosed.
    Type: Grant
    Filed: June 6, 1995
    Date of Patent: June 18, 2002
    Assignee: Dade Behring Marburg GmbH
    Inventors: Edwin F. Ullman, Hrair Kirakossian, John S. Pease, Yuri Daniloff, Daniel B. Wagner
  • Publication number: 20020052471
    Abstract: The invention relates to human procalcitonin and the preparation and use thereof. In particular, a process for preparing human procalcitonin is described wherein a gene coding for a polypeptide comprising the amino acid sequence of human procalcitonin is inserted into a vector; a host organism is transformed with this gene-containing vector; and the polypeptide expressed by the host organism is isolated. Furthermore the use of the polypeptides according to the invention, in particular as medicaments and diagnostic agents is described.
    Type: Application
    Filed: December 22, 2000
    Publication date: May 2, 2002
    Applicant: Dade Behring Marburg GmbH.
    Inventors: Harald Althaus, Hans-Peter Hauser
  • Publication number: 20020050147
    Abstract: The invention relates to containers for the dispatch and storage of commodities under controlled temperature conditions, in which the refrigerating or heating agents used for maintaining a specific temperature range can be introduced, spatially separated from the commodities in the interior of the container by one or more separators.
    Type: Application
    Filed: October 5, 2001
    Publication date: May 2, 2002
    Applicant: Dade Behring Marburg GmbH
    Inventors: Hans-Joachim Mai, Hans-Jurgen Weber
  • Patent number: 6376209
    Abstract: The present invention relates to a process for increasing the FVII sensitivity of a thromboplastin reagent by means of heat treatment.
    Type: Grant
    Filed: May 15, 1998
    Date of Patent: April 23, 2002
    Assignee: Dade Behring Marburg GmbH
    Inventors: Thomas Wissel, Hermann Keuper, Hubert Nettelhoff, Heinz-Georg Kandel, Reiner Muth, Michael Kraus
  • Patent number: 6372426
    Abstract: The present invention relates to a method and a diagnostic aid for the qualitative or quantitative detection of antibodies and for determining their avidity. This makes it possible to diagnose the early phase of viral, bacterial or parasitic infections. The diagnostic aid according to the invention is particularly suitable for automated processing in large analytical laboratories.
    Type: Grant
    Filed: July 24, 2000
    Date of Patent: April 16, 2002
    Assignee: Dade Behring Marburg GmbH
    Inventor: Wolfgang Zens
  • Patent number: 6372423
    Abstract: The present invention relates to preparations of functionally active platelets and to a process for stabilizing these functionally active platelets by means of freeze-drying. In particular, a lyophilized or reconstituted platelet composition containing an anticoagulant, a cake forming agent, and an inhibitor of platelet function is disclosed.
    Type: Grant
    Filed: June 7, 2000
    Date of Patent: April 16, 2002
    Assignee: Dade Behring Marburg GmbH
    Inventor: Konrad Braun
  • Publication number: 20020012958
    Abstract: The present invention relates to a process for increasing the FVII sensitivity of a thromboplastin reagent by means of heat treatment.
    Type: Application
    Filed: May 15, 1998
    Publication date: January 31, 2002
    Applicant: Dade Behring Marburg GmbH
    Inventors: THOMAS WISSEL, HERMANN KEUPER, HUBERT NETTELHOFF, HEINZ-GEORG KANDEL, REINER MUTH, MICHAEL KRAUS
  • Patent number: 6340599
    Abstract: Compositions are disclosed comprising (a) a metal chelate wherein the metal is selected from the group consisting of europium, terbium, dysprosium, samarium osmium and ruthenium in at least a hexacoordinated state and (b) a compound having a double bond substituted with two aryl groups, an oxygen atom and an atom selected from the group consisting of oxygen, sulfur and nitrogen wherein one of the aryl groups is electron donating with respect to the other. Such composition is preferably incorporated in a latex particulate material. Methods and kits are also disclosed for determining an analyte in a medium suspected of containing the analyte. The methods and kits employ as one component a composition as described above.
    Type: Grant
    Filed: May 11, 1998
    Date of Patent: January 22, 2002
    Assignee: Dade Behring Marburg GmbH
    Inventors: Sharat Singh, Edwin F. Ullman
  • Patent number: 6337182
    Abstract: Method for the Quantitative Determination of DNA Sequences A method for the quantitative determination of DNA sequences containing at least one mutationally eliminated restriction site is disclosed in which the DNA sequences to be quantitatively determined are selectively amplified continuously eliminating any residual wild-type DNA sequences.
    Type: Grant
    Filed: June 6, 1991
    Date of Patent: January 8, 2002
    Assignee: Dade Behring Marburg GmbH
    Inventors: Peter A. Cerutti, Emanuela Felley-Bosco, Martha Sandy, Paul Amstad, Jacob Zijlstra, Charareh Pourzand
  • Patent number: 6335158
    Abstract: The present invention is directed toward nucleic-acid based methodologies for the detection of human immunodeficiency virus (HIV) nucleic acids in a sample. A novel HIV-1 isolate, designated MVP5180/91, was isolated from a West African Cameroonian patient with immunodeficiency. Nucleic acid and amino acid sequence comparisons of this isolate, with other HIV-1 strains of subtypes A-E and HIV-2 isolates, demonstrated that this virus shares only limited homology with other known HIV-1 and -2 isolates. However, this virus does display some genetic relatedness to another Cameroonian isolate designated ANT-70. These viruses form the basis for a new HIV-1 group which has been designated subtype O. An immunologically important epitope, corresponding to amino acids 601-623 of the MVP5180/91 transmembrane envelope glycoprotein, was identified. Labeled nucleic acids can be prepared from the nucleotide sequence encoding this region and employed in standard hybridization assays to detect HIV-1 nucleic acids.
    Type: Grant
    Filed: August 10, 1998
    Date of Patent: January 1, 2002
    Assignee: Dade Behring Marburg GmbH
    Inventors: Stefan Brust, Stefan Knapp, Manfred Gerken, Lutz G. Guertler
  • Publication number: 20010049146
    Abstract: The present invention relates to the use of sterol esters for the long-term stabilization of biological fluids, in particular even those which are obtained by lyophilization and subsequent reconstitution.
    Type: Application
    Filed: October 29, 1999
    Publication date: December 6, 2001
    Applicant: Dade Behring Marburg, GmbH
    Inventor: HARALD ALTHAUS
  • Patent number: 6326008
    Abstract: The present invention relates to the identification of toxoplasma gondii antigens and the preparation thereof by genetic engineering. A cDNA expression gene bank of this parasite was prepared. Recombinant clones which are of diagnostic interest were identified using a high-titer rabbit anti-Toxoplasma gondii serum, and isolated.
    Type: Grant
    Filed: December 16, 1999
    Date of Patent: December 4, 2001
    Assignee: Dade Behring Marburg GmbH
    Inventors: Stefan Knapp, Robert Ziegelmaier, Hans Küpper
  • Patent number: 6326159
    Abstract: Receptors are disclosed that are antibodies that exhibit a binding affinity for an immune complex of a monoepitopic antigen and an antibody for such antigen that is substantially greater than the binding affinity for the monoepitopic antigen or the antibody for the monoepitopic antigen apart from the immune complex. Normally, the monoepitopic antigen has a molecular weight less than 1500 and is an organic compound. The antibodies of the present invention find use in a method for determining a monoepitopic antigen in a sample suspected of containing such antigen. The method comprises forming an immune sandwich complex comprising the monoepitopic antigen or an analog thereof, a first monoclonal antibody that binds to the monoepitopic antigen, and a second monoclonal antibody that is an antibody of the present invention and detecting the immune sandwich complex.
    Type: Grant
    Filed: September 2, 1994
    Date of Patent: December 4, 2001
    Assignee: Dade Behring Marburg GmbH
    Inventors: Edwin F. Ullman, John Jelesko, Marcel R. Pirio, Thomas D. Kempe
  • Patent number: 6322794
    Abstract: The invention relates to seroreactive epitopes on proteins of human papillomavirus HPV18. In addition, the invention relates to peptides which have amino-acid sequences which coincide in whole or in part with the sequences of the seroreactive epitopes, and to vaccines which contain such peptides.
    Type: Grant
    Filed: December 10, 1993
    Date of Patent: November 27, 2001
    Assignee: Dade Behring Marburg GmbH
    Inventors: Conrad Bleul, Lutz Gissmann, Martin Müller
  • Patent number: 6319690
    Abstract: The invention relates to the preparation and use of gene banks of human antibodies (Ab). Starting from a mixture of human B-lymphocytes, their mRNA is translated into the cDNA using oligo-dT primers. Subsequently, an amplification of the Ab-specific cDNA by means of polymerase chain reaction (PCR) takes place using suitable oligonucleotide primer sequences. Expression of this amplified Ab-specific cDNA in a bacterial expression vector, e.g. the vector pFMT described below, in E. coli thus makes available a human-antibody library with a comprehensive repertoire for screening selected antigens in vitro.
    Type: Grant
    Filed: May 6, 1993
    Date of Patent: November 20, 2001
    Assignee: Dade Behring Marburg GmbH
    Inventors: Melvyn Little, Frank Berthold Breitling, Thomas Seehaus, Stefan Dübel, Iris Klewinghaus
  • Patent number: 6317702
    Abstract: The present invention relates to a method for instrument determination of measured variable L(t) which changes with time, the intention being to determine the maximum rate value of L(t) in a region with a linear reaction profile, and the time as well as the duration of the reaction time window being variable with the linear region and depending on the nature of the reaction and the reaction conditions. Specifically, the present invention relates to the determination of protein concentrations with the aid of light scatter, which is produced by specific antibodies, in homogeneous solutions. In particular, the invention relates to reactions which take place slowly and have a largely linear profile over a relatively long time, the rate of formation of antigen-antibody complexes in the linear section of the reaction (VMaxLin) being determined as the measured variable.
    Type: Grant
    Filed: February 11, 2000
    Date of Patent: November 13, 2001
    Assignee: Dade Behring Marburg GmbH
    Inventor: Jürgen Patzke
  • Patent number: RE37596
    Abstract: The invention relates to monoclonal antibodies (MAbs) and fragments thereof which bind to defined tumor-associated antigens, principally of small cell lung carcinoma (SCLC), of melanoma, of neuroblastoma and other tumors of neuroectodermal origin, to hybridoma cell lines for the preparation thereof, and to the antigens which can be defined and/or isolated with the aid of these antibodies or antibody fragments. The antibodies, antibody fragments and antigens can be used as diagnostic, aid, active substance or active substance carrier.
    Type: Grant
    Filed: June 16, 1999
    Date of Patent: March 19, 2002
    Assignee: Dade Behring Marburg GmbH
    Inventors: Klaus Bosslet, Bernhard Auerbach, Helmut Peters