Abstract: The present invention relates to mammalian cells modified to provide for improved expression of a recombinant protein of interest. In particular, the invention relates to CHO cells and other host cells in which the expression of one or more endogenous secreted proteins has been disrupted, as well as to the preparation, identification and use of such cells in the production of recombinant proteins.

Abstract: For diagnostic purposes, and particularly point-of-care diagnostic purposes, there is a need for devices capable of detecting quorum sensing molecules such as AHL within a biological sample with high precision, and which furthermore are fast and simple to use. The present invention relates to an electrochemical device, comprising: 5 —at least one reference electrode (RE), —at least one counter electrode (CE), —two or more working electrodes (WEs), wherein each working electrode differ from the other working electrode(s) with respect to at least one of the following characteristics: surface area, 10 size, material, and coating, —a sample receiving area for receiving a biological sample, wherein the electrodes and the sample receiving area is fluidly connected —means for transferring the sample to the electrodes for measurement, and —means for displaying a result of the measurement.

Abstract: Solvent consumption in supercritical ethanol, propanol or butanol treatment of either refined pre-extracted lignin or comparatively impure lignin-rich solid residual from hydrothermally pretreated lignocellulosic biomass can be minimized by conducting the reaction at very high loading of lignin to solvent. Comparatively impure, crude lignin-rich solid residual can be directly converted by supercritical alcohol treatment to significantly diesel-soluble lignin oil without requirement for pre-extraction or pre-solubilization of lignin or for added reaction promoters such as catalysts, hydrogen donor co-solvents, acids, based or H2 gas. O:C ratio of product oil can readily be obtained using crude lignin residual in such a process at levels 0.20 or lower.

Abstract: The invention relates to a method for producing zeolite, zeolite-like or zeotype particles comprising the steps of: 1) Adding one or more metal precursors to a silica or alumina source; 2) Reducing the one or more metal precursors to form metal nanoparticles on the surface of the silica or alumina source; 3) Passing a gaseous hydrocarbon, alkyl alcohol or alkyl ether over the silica or alumina supported metal nanoparticle to form a carbon template coated zeolite, zeolite-like or zeotype precursor composition; 4a) Adding a structure directing agent to the carbon template coated zeolite, zeolite-like or zeotype precursor composition thereby creating a zeolite, zeolite-like or zeotype gel composition; 4b) Crystallizing the zeolite, zeolite-like or zeotype gel composition by subjecting said composition to a hydrothermal treatment; 5) Removing the carbon template and structure directing agent and isolating the resulting zeolite, zeolite-like or zeotype particles.

Abstract: The invention therefore relates to a method for producing zeolite, zeolite-like or zeotype encapsulated metal nanoparticles, the method comprises the steps of: 1) Adding one or more metal precursors to a silica or alumina source; 2) Reducing the one or more metal precursors to form metal nanoparticles on the surface of the silica or alumina source; 3) Passing a gaseous hydrocarbon, alkyl alcohol or alkyl ether over the silica or alumina supported metal nanoparticles to form a carbon template coated zeolite, zeolite-like or zeotype precursor composition; 4a) Adding a structure directing agent to the carbon template coated zeolite, zeolite-like or zeotype precursor composition thereby creating a zeolite, zeolite-like or zeotype gel composition; 4b) Crystallising the zeolite, zeolite-like or zeotype gel composition by subjecting said composition to a hydrothermal treatment; 5) Removing the carbon template and structure directing agent and isolating the resulting zeolite, zeolite-like or zeotype encapsulated meta

Abstract: The present invention relates to isomerization of C4-C6 aldoses to their corresponding C4-C6 ketoses. In particular, the invention concerns isomerization of C4-C6 aldoses over solid zeolite catalysts free of any metals other than aluminum, in the presence of suitable solvent(s) at suitable elevated temperatures. C6 and C5 aldose sugars such as glucose and xylose, which are available in large amounts from biomass precursors, are isomerized to fructose and xylulose respectively, in a one or two-step process over inexpensive commercially available zeolite catalysts, containing aluminum as the only metal in the catalyst. The ketoses obtained are used as sweeteners in the food and/or brewery industry, or treated to obtain downstream platform chemicals such as lactic acid, HMF, levulinic acid, furfural, MMHB, and the like.

Abstract: The invention described materials and methods for producing oxidized 13R-MO, such as forskolin. In particular, the invention describes P450s involved in oxidation of 13R-MO including CYP76AH8, CYP76AH11, CYP76AH15, CYP76AH17, CYP71D381 and CYP76AH9. Host organisms expressing one or more of these P450s are useful in the production of oxidized 13R-MO.

Abstract: An anchoring device for anchoring tendons for structural reinforcement. The anchoring device has a longitudinal central axis defining an axial direction. The anchoring device includes an outer barrel and an inner wedge. The outer barrel has a cylindrically or frusto-conically shaped inner surface defining a cylindrically or frusto-conically shaped inner space. The inner wedge has a frusto-shaped outer surface and a coaxial bore. The shaped inner space is configured for allowing the inner wedge to be positioned in the shaped inner space of the outer barrel in the axial direction. The coaxial bore of the inner wedge is configured for receiving a tendon. The inner wedge includes inner and outer portions, the inner portion overlapping the outer portion as seen in a radial direction.

Abstract: The present disclosure describes a device and corresponding method for measuring tar in a tar environment, e.g., a tar producing environment such as a stove or a combustion engine, based on UV absorption spectroscopy. A first measurement along an optical path in the tar environment is performed at a wavelength less than 340 nm at which both tar and non-tar elements absorb. This measurement is compensated for non-tar absorption by means of a second measurement at a wavelength equal to or greater than 340 nm at which tar does not absorb. From the non-tar compensated absorbance value a measure of tar in the tar environment is derived and an air intake in the tar environment is regulated based on the measure of tar.

Abstract: The present invention relates to a new strategy for capturing NOx using a two-step process.

Abstract: An isolated glycosyltransferase (GT) polypeptide capable of: (I): conjugating glucose to flavokermesic acid (FK); and/or (II): conjugating glucose to kermesic acid (KA) and use of this GT to e.g. make Carminic acid.

Abstract: The present invention relates to a rotor for a pyrolysis centrifuge reactor, said rotor comprising a rotor body having a longitudinal center axis, and at least one pivotally mounted blade being adapted to pivot around a pivot axis under rotation of the rotor body around the longitudinal center axis. Moreover, the present invention relates to a pyrolysis centrifuge reactor applying such a rotor.

Abstract: The present invention relates to a method for hydrogenolysis of alpha-hydroxy esters or acids, comprising reacting the alpha-hydroxy ester or acid in the presence of a heterogeneous catalyst. The present invention also relates to a method for producing propionic acid ester, and the use of any of the methods for the production of propionic acid esters, such as alkyl propionate.

Abstract: A step-up DC-DC power converter comprises a primary side circuit and a secondary side circuit coupled through a galvanic isolation barrier. The primary side circuit comprises a positive and a negative input terminal for receipt of an input voltage and an input capacitor coupled between the positive and negative input terminals and the secondary side circuit comprises an output capacitor chargeable to a converter output voltage between a first positive electrode and a second negative electrode. The galvanic isolation barrier comprises a first capacitor coupled in series with the positive input terminal of the primary side circuit and the first positive electrode of the output capacitor; and a second capacitor coupled in series with the negative input terminal of the primary side circuit and the second negative electrode of the output capacitor.

Abstract: The present invention relates to a method for manufacturing an at least partly hollow MEMS structure. In a first step one or more through-going openings is/are provided in core material. The one or more through-going openings is/are then covered by an etch-stop layer. After this step, a bottom electrically conducting layer, one or more electrically conducting vias and a top electrically conducting layer are created. The bottom layer is connected to the vias and vias are connected to the top layer. The vias are formed by filling at least one of the one or more through-going openings. The method further comprises the step of creating bottom and top conductors in the respective bottom and top layers. Finally, excess core material is removed in order to create the at least partly hollow MEMS structure which may include a MEMS inductor.

Abstract: The present disclosure relates to a filament stretching rheometer for measuring rheological and/or mechanical properties of a sample, comprising: a pair of opposed surfaces for holding the sample therebetween; an actuator configured to provide a controlled axial displacement of at least one of said opposed surfaces; and a sample scanning unit for measuring a diameter of said sample, the sample scanning unit configured for measuring said sample diameter at an axial position controlled independently of the displacement of the opposed surfaces, the sample scanning unit configured for being positioned at a starting point before said controlled axial displacement, wherein the starting point is selected from a position where a minimum diameter of the said sample is determined by said sample scanning unit.

Abstract: The present invention relates to an optical measuring system comprising a polymer-based single-mode fibre-optic sensor system (102), an optical interrogator (101), and an optical arrangement (103) interconnecting the optical interrogator (101) and the polymer-based single-mode fibre-optic sensor system (102). The invention further relates to an optical interrogator adapted to be connected to a polymer-based single-mode fibre-optic sensor system via an optical arrangement. The interrogator comprises a broadband light source arrangement (104) and a spectrum analysing arrangement which receives and analyses light reflected from the polymer-based single-mode fibre-optic sensor system.

Abstract: The present invention concerns the absorption and desorption behavior of carbon dioxide (CO2) using ionic liquids derived from amino acids adsorbed on porous carrier materials.

Abstract: The invention provides a genetically modified micro-organism for intracellular biosynthesis of a cellular metabolite, comprising a synthetic error correction system having a penalty gene, whose expression leads to arrested growth or cell death (e.g. a toxin gene) in combination with a survival gene, whose expression provides an antidote that restores cell viability and normal growth (e.g. a cognate antitoxin gene). Alternatively, the system has a survival gene, alone, whose expression is essential for growth (i.e. essential gene). The synthetic error correction system further comprises a biosensor, whose function is to induce expression of the survival gene which leads to cell growth, only, when the cell produces a pre-defined level of a given metabolite.

Abstract: The present disclosure relates to a switched capacitor DC-DC converter configured for converting a DC input voltage into a higher or lower DC output voltage. The switched capacitor DC-DC converter comprises an output voltage regulator utilizing a feedback loop with a multi-level quantizer configured to convert a lowpass filtered control signal into a corresponding digital control signal.