Abstract: The present invention relates to a method for in-situ synthesis of alkyl esters of fatty acids and their efficient extraction from oleaginous microbes. Particularly, the present invention provides a rapid method for downstream processing of oleaginous biomass for in-situ synthesis of alkyl esters of fatty acids and their efficient extraction. The method of the present invention results in increased transesterification efficiency (>95%) of FAAE production from wet biomass, while eliminating the necessity of biomass harvesting, drying and large requirement of chemical solvents.

Abstract: The present disclosure relates to a process for producing levulinic acid from the technical lignin. The technical lignin is rich in lignin and ash and do not have any other use and usually incinerated as a waste management strategy. During the incineration process, the cellulose also gets burnt and wasted.

Abstract: The present invention includes Uttroside B compositions and method for the treatment of hepatocellular carcinoma. Chemotherapeutic options for liver cancer are limited and the prognosis of HCC patients remains dismal. Sorafenib, is the only drug currently available for the treatment of hepatocellular carcinoma.

Abstract: The invention relates to the domain of microscope based imaging. The invention provides methods and apparatuses for providing improved microscope based digital imaging solutions that are capable of providing high quality images with a high level of image detail. The invention additionally provides solutions for artificial intelligence based controlling of a digital microscope's imaging functions to enable bright field/dark field imaging functionality to be combined with spectroscopic functions to obtain higher detail and more meaningful information about a specimen sample.

Abstract: An integrated approach for utilizing waste products of 2G bio-refineries to fractionate the lignin of high purity. The present invention also provides a method of recycling of two waste products (2-G ethanol residue as the substrate (LRBR) and fusel oil/synthetic fusel oil (SFO) as one of the solvent) of the biorefineries in a beneficial manner to fractionate the lignin of high purity. The present method of separating high purity lignin comprises fractioning extractive-free lignin rich residue with a solvent, wherein the solvent is a mixture of SFO/Fusel oil and Formic acid. An optimized ratio of waste fusel oil and formic acid gave more than >85% yields of high purity lignin.

Abstract: The present invention discloses a process of xylitol production from pure xylose, xylose containing liquid extract from the acid pretreated biomass (LEPB) and co-production of ethanol and xylitol from dilute acid pretreated lignocellulosic biomass by natural S. cerevisiae DBT-IOC S24 (MTCC25086). The present invention also discloses a process of xylitol production wherein a thermo and inhibitor tolerant S. cerevisiae DBT-IOC S24 (MTCC25086) natural strain has been induced to produce xylitol from xylose at defined process parameters using xylose containing diluted acid pretreated biomass as well as from xylose containing synthetic media in presence of glucose, lignocelluloses biomass derived xylitol inducer maintaining aeration at 1VVM and pH at 5.0 in the medium.

Abstract: The present invention includes Uttroside B compositions and method for the treatment of hepatocellular carcinoma. Chemotherapeutic options for liver cancer are limited and the prognosis of HCC patients remains dismal. Sorafenib, is the only drug currently available for the treatment of hepatocellular carcinoma.

Abstract: A multi-action wound dressing for accelerated wound healing by means of multi-therapeutic action is disclosed. The dressing comprises a porous sheet (101); two flexible sheets (102) having a first sheet (103) and a second sheet (104) attached to each other; multichannel conduits (105) or a plurality of single channel conduits; multichannel tubes (106), side adhesive tapes (107) and an optional wound contact layer (108). The porous sheet (101) includes a top planar surface (201), thickness (202) and a bottom uneven surface (203). The bottom uneven surface (203) lies on the surface of the wound and may have surface patterns (204). The pattern (204) may be wavy patterns and/or any other regular and/or irregular surface protrusion that allow intermediate gaps between wound surface and the bottom surface (203) of the porous sheet through which fluid can flow over the wound surface.

Abstract: A device including a probe unit and a driver unit for tissue extraction and sample collection is disclosed. The probe unit includes an injection unit, a driving mechanism, a filtering mechanism and a tissue sample container. The injection unit includes a needle actuated by a prime-mover and the driving mechanism, which drives the needle into the subject for extraction of tissue sample. The tissue extraction is performed by a rotational motion of the needle and pressure. The pressure is provided by a pressure subsystem in the lumen of the needle and the tissue is collected in the tissue sample container. The amount of pressure applied is controlled by a control subsystem based on the organ specific requirement.

Abstract: The present invention relates to a mutant fungal strain of Penicillium funiculosum ‘MRJ-16’ characterized by the ability to produce high titer of enzyme mixture comprising FPase, CMCase, Cellobiase, ?-glucosidase, endoglucanase, ?-L arabinofuranosidase, ?-xylosidase, xylanase, pectinase, cellobiohydrase and oxidases and produce enzymes in the presence of a catabolite repressor molecule like glucose and/or xylose. The titer of enzyme mixture produced using mutant fungal strain MRJ-16 is at least two fold higher than naive Penicillium funiculosum strain NCIM 1228, when used in a fermentation process. The mutant strain ‘MRJ-16’ with high hydrolytic activity and catabolite derepressed character is having application in the method of degrading or saccharifying biomass to produce valuable products for example-bioethanol.

Abstract: The present invention provides a breathable orthopedic cast device for immobilizing and stabilizing at least one fractured limb. The breathable orthopedic cast device comprising: a customizable cast member having hollow tubes pre-injected with at least one infusion material, wherein hollow tubes are interlinked with each other to form a mesh structure to provide breathability to a fractured limb. The breathable orthopedic cast device is a flat unfolded geometry that can be applied by wrapping over an injured body member, or is customized to a shape corresponding to the contours of the body member.

Abstract: An integrated approach for utilizing waste products of 2G bio-refineries to fractionate the lignin of high purity. The present invention also provides a method of recycling of two waste products (2-G ethanol residue as the substrate (LRBR) and fusel oil/synthetic fusel oil (SFO) as one of the solvent) of the biorefineries in a beneficial manner to fractionate the lignin of high purity. The present method of separating high purity lignin comprises fractioning extractive-free lignin rich residue with a solvent, wherein the solvent is a mixture of SFO/Fusel oil and Formic acid. An optimized ratio of waste fusel oil and formic acid gave more than >85% yields of high purity lignin.

Abstract: The present invention relates to a method for obtaining a high titer of enzyme mixture comprising cellulases, hemicellulases and ?-glucosidases in reutilization of waste water generated during hot water extraction of lignocellulosic biomass or biorefinery waste water using Penicillium funiculosum MRJ-16 mutant strain. The cellulose or lignocellulosic biomass used in the fermentation process is selected from the group consisting of rice straw, wheat straw, corn stover, cotton stalk or a combination thereof. The enzyme mixture obtained by the present process is used for the saccharification of acid pretreated lignocellulosic biomass.

Abstract: The present invention relates to an improved method of second generation ethanol production from a lignocellulosic biomass. The process comprises subjecting a slurry of pre-treated lignocellulosic biomass comprising C5 and C6 sugars in a fermentor; preferentially fermenting mainly C5 sugars by incubating the pretreated lignocellulosic biomass of step (i) with first cellulase enzyme, co-fermenting microorganism, nutrient in the fermentor at 30-37° C. for a period of 16 to 24 hours; adding second cellulase enzyme to the fermentation broth of step (ii) and increasing the temperature to 45-55° C. for hydrolysis; allowing the fermentation broth of step (iii) to cool to a temperature of 35-38° C.; and preferentially fermenting C6 sugars by incubating the broth of step (iv) with a second dose of co-fermenting microorganism for a period of 6-8 hours to obtain ethanol. The process results in high ethanol productivity in shortest duration of time.

Abstract: An advantageous process for pre-treating lignocellulosic biomass to hydrolysable polysaccharide enriched biomass with reduced amount of inhibitors of catalysis comprising steps of: providing a feedstock comprising cellulosic biomass; chopping of cellulosic biomass to have a cellulosic biomass feed material of uniform size; feeding of uniform sized feed material to a horizontal counter current extraction unit; removing excess of water from the feed material obtained from the extraction unit; soaking the extruded feed material obtained from the extraction unit; optionally removing excess of water from the feed material obtained after acid soaking; steam pre-treating the feed material obtained after removing excess of water from the acid soaked feed material; neutralizing the slurry obtained from the steam pre-treating; and optionally saccharifying the neutralized slurry.

Abstract: The present invention relates to a process for simultaneous production of citric acid and cellulolytic enzymes. The batch process comprising (i) adding slurry of a pre-treated lignocellulosic biomass or cellulose in a fermentation media; (ii) inoculating 10% (v/v) active liquid seed culture of Penicillium funiculosum MRJ-16 in the fermentation media of step (i); (iii) subjecting the culture of step (ii) to fermentation in an aerated fermenter at a temperature of 28-33° C. for a duration of 96 hours; and (iv) collecting enzyme broth after fermentation of step (iii) to obtain citric acid and cellulolytic enzymes. The batch and fed-batch process of the present invention results in high titer production of citric acid and cellulolytic enzymes in a single step and using single microbial strain.

Abstract: The present invention discloses a recombinant, modified extracellular chitinase having an amino acid sequence set forth in SEQ ID NO.4 or SEQ ID NO.5. The recombinant, modified chitinase is derived by inserting two-point mutations at positions 661 and 2158 in the native chitinase gene of Brevibacillus laterosporus LAK 1210 which results in amino acid substitution of tyrosine (Y) residue with histidine (H) at positions 221 and 720. The modified chitinase exhibits both exochitinase and endochitinase activity. The recombinant, modified enzyme is thermoactive with a temperature optimum of 55-60° C. and high thermostability (Tm of 66.7° C.), functions in a broad pH range (pH 3.0-11.0) having a pH optimum of 9.0 and also exhibits improved solubility and enhanced efficacy for control of insects and phytopathogenic fungi.

Abstract: A multifunctional chemical agents comprising functional agents Fn1, Fn2 and linkers, for the linchpin directed (LDM), protein directed (PDPM) modifications of proteins, and Fn1 accelerated kinetic labeling by Fn2.

Abstract: The present invention relates to a method of cellulases enzymes production by Penicillium funiculosum MRJ-16 using minimum media components and/or low cost media components like cellulose or pretreated lignocellulosic biomass as carbon source and soya flour or defatted soya flour or de-oiled soya cake as nitrogen source. The present invention also provides a method for production of high titer of cellulases and hemicellulases enzymes using Penicillium funiculosum MRJ-16 using minimum media and/or low cost media components.

Abstract: A multi-dimensional micro fluid focussing device. The device includes an apparatus for multi-dimensional micro fluid focussing. The device also includes an analyser for analysing the focused fluid. The apparatus includes a microchannel having an inlet defining a first region, a middle region and an outlet defining a second region. A first junction is formed at the intersection of the first region and the middle region. A second junction is formed at the intersection of the middle region and the second region. A first sheath positioned proximal to the first junction and a second sheath positioned at the second junction. The junctions formed, along with the positioning of the sheaths enable the multi-dimensional focusing of the fluid. The analyser includes a holder for removably retaining the apparatus. A microscope is positioned across the holder. A recording unit is coupled to the microscope.