Abstract: A method of preparing dialysed aqueous extract of fenugreek seeds comprising washing the fenugreek seeds is distilled water, sterilizing the said seeds, subjecting the sterilized seeds to the step of grinding to form powder, suspending the said powder in phosphate buffered saline (PBS), subjecting the said suspension to the step of filtration to obtain the filtrate, treating the filtrate with activated charcoal to obtain clear supernatant, subjecting the supernatant to the step of lyophilization and the powder thus obtained was dissolved in phosphate buffered saline (PBS), dialyzing the aqueous extract of fenugreek seeds to obtain dialysed fenugreek seed extract (FSE) which was aliquoted and stored.

Abstract: Maintenance of quiescent hematopoietic stem and progenitor cells (HSPC) in culture without the addition of exogenous growth factors is an important aspect in clinical hematology. A recent report described the ability of Flt3 receptor-interacting lectin (FRIL) in the maintenance of cord blood (CB) derived progenitors in vitro. Since FRIL is a mannose binding lectin, the effectiveness of four such lectins of well-characterized specificities on the preservation of HSPC of CB origin have been examined. The HSPC preservation activity of lectins was assessed by in vitro colony forming unit (CFU) and long-term culture initiating cell (LTC-IC) assays. It was found that all four lectins had a HSPC preservation activity at least up to 30 days in culture without addition of exogenous growth factors. The results indicate that use of such lectins may provide a cost effective method of HSPC maintenance for clinical use.

Abstract: A collector for fecal discharge is provided comprising a self-expanding resilient collection component, a housing sheath and a transit component. The collection component has an open proximal and distal end and a lumen connecting them. The component comprises interconnected resilient arms circumscribing its contour, wherein adjacent arms are resiliently biased away from each other for exerting outwardly radial pressure for expanding and anchoring the component to rectal walls upon deployment. The housing sheath comprises a flexible and resilient material overlaying at least one of inner or outer contour of collection component without interfering with or blocking its ends or lumen. The transit component provides a conduit for fecal discharge to migrate from collection component to a receptacle and comprises a flexible, tubular sheath having a first open end connected to a second end by a lumen, wherein the first end engages with the proximal end of the collection component.

Abstract: Provided herein is a modified method of agglutination to detect infections caused by microorganisms including the steps of staining the test serum, plasma or blood or purified antibodies with a protein stain; mixing serum, plasma or blood with stained antibodies with an equal quantity of colored antigen particles on a glass slide; adding diluted Antiglobulin conjugated with Biotin to the mixture; subjecting the mixture to the step of mixing, adding diluted Avidin (preferably tagged with a visible indicator) to the mixture and thoroughly mixing all the ingredients.

Abstract: This invention relates to a technique to enhance the clinical efficiency of radiotherapy and readiosurgery using perturbative beaming and tissue specific radiobiology, comprising, Considering the normal steady beam dose rate, then increasing the temporal stochastic fluctuation of the output intensity of the radiation beam from a medical linear accelerator or cobalt gamma knife beam setup, developing a computer-based methodology by considering the tumor specific radiobiology and magnitude of the tailor made dose rate fluctuation using an embedded system device consisting of requisite hardware and software as described herein.

Abstract: A method for medical image enhancement based on image transform resonance. A novel method for Stochastic Resonance of medical image enhancement device based on the Integral Transform of the image, comprising: generating specific types of integral transforms like radon transform for CT, Fourier transform for MRI with image transform component; preparing stochastic perturbation waveform, with perturbator component performing the stochastic resonance on the image transform, with stochastic resonator component; characterizing the image enhancement factor of the SR-processed image with performance monitoring component; adjusting and controlling the bistability parameters of the double-well system that induces stochastic resonance with control component; arranging the provisional display of the array matrix of the SR-enhanced images, as the bistability parameters are varied with matrix display component; subjecting the final image to the step of display with final image display component.

Abstract: This invention relates to a method for detecting, (i) malignant astrocytoma from normal brain tissue (ii) glioblastoma and anaplastic astrocytoma (iii) primary glioblastoma and secondary glioblastoma (iv) progressive pathway and denovo pathway comprises determining the level of expression of miRNAs listed in table 2, 3, 4, 5, wherein a higher or lower level of expression of miRNAs in the test sample as compared to the control sample differentiates and kit for characterizing a) malignant astrocytoma from normal brain tissue cell comprising reagent capable of specifically detecting the level of expression of the genes of miRNAs and instructions for using said kit for characterizing malignant astrocyoma from normal brain tissue cells b) glioblastoma from anaplastic astrocytoma comprising reagent capable of specifically detecting the level of expression of the genes of miRNAs and instructions for using said kit for characterizing glioblastoma from anaplastic astrocyoma c) primary glioblastoma from secondary gliob

Abstract: The invention relates to a fully automated screening, diagnosis and classification technique for Alzheimer's disease using magnetic resonance imaging signals from the ventricular zone contour of the brain to get a fundamental index of brain deterioration comprising the steps: —obtaining a gray scale MRI image of the brain region; —applying a contour edge-detecting algorithm to the image; —employing a grid covering method for calculating a first order metric index of ventricular zone contour; —superimposing metric square grids of increasing edge length to the binary contour image and counting the metric grid squares; —plotting the logs of metric grid squares against the logs of edge lengths, wherein the gradient of the plot is the linear topological metric index.

Abstract: A novel chemical moiety triterpenoid AECHL-1 isolated from root bark of Ailanthus excelsa having the structure:

Abstract: A process for affecting reductive conversion reactions in a reactor using the palladized bacterial cellulose immobilized on a support for reductive conversion reactions in a reactor.

Abstract: Chimeric tumor suppressor activating peptides derived from matrix attachment region binding protein (MARBP) SMARI unique in their sequence comprising a arginine rich motif flanked by serine residues wherein from the stretch of four consecutive serine residues the first serine residue gets phosphorylated by the protein kinase C family of serine threonine kinases being indispensable for its functionality, the phosphorylation being directly correlated to the phosphorylation of p53 at serine 15 residue thereby stabilizing it, wherein the peptide activates p53 by modifying it post translationally which allow phosphorylation and translocation of p53 to the nucleus.

Abstract: A process for generating transgenic animals using recombinant lentiviruses. The process comprises injecting recombinant lentiviruses into the interstituim of the testis of a male to produce mature spermatozoa within a few days. The male with transgene expressing lentivirus is mated with a female, forming a progeny carrying the transgene.

Abstract: This invention relates to a simple end point assay for detection of transient intracellular Ca2+ with broad applicability to many Ca2+ channel proteins comprising, Generation of expression constructs for the fusion proteins having the Ca2+/calmodulin dependent protein kinase II (CaMKII) phosphorylation sites of NR2A or NR2B subunits of N-methyl-D-aspartate receptor (NMDAR) or the voltage gated potassium channel of Drosophila (Eag) or any protein sequence which binds to the T-site of CaMKII similar to NR2B, conjugated to mitochondrial localizing signal sequence, or mutants of these sequences as described herein. Generation of mammalian expression constructs of ?-CaMKll as a chimera with green fluorescent protein (GFP-?-CaMKII) or its mutants as described herein.

Abstract: A novel recombinant chimera of DNA construct having esat-6 region of Mycobacterium tuberculosis and kinesin region of Leishmania donovani cloned together on two sides of self cleaving peptide in a DNA vaccine vector pVAX-1 wherein the chimeric construct is operatively linked to a transcriptional promoter thus capable of self replication and expression within the mammalian cell, and the process of preparation thereof comprising: analysis of the predicted protein sequence of kinesin motor domain and esat-6 domain using Promiscuous MHC Class-1 Binding Peptide Prediction Servers; amplification of gene coding for kinesin motor domain and esat-6 domain; cloning of kinesin esat-6 gene region in pGEM-T™ vector for sequence analysis; generation of chimeric construct by directional cloning in pVAX-1 vector.

Abstract: Provided herein is a modified method of agglutination to detect infections caused by microorganisms including the steps of staining the test serum, plasma or blood or purified antibodies with a protein stain; mixing serum, plasma or blood with stained antibodies with an equal quantity of colored antigen particles on a glass slide; adding diluted Antiglobulin conjugated with Biotin to the mixture; subjecting the mixture to the step of mixing, adding diluted Avidin (preferably tagged with a visible indicator) to the mixture and thoroughly mixing all the ingredients.

Abstract: This invention is a process for the preparation of talc based formulation for LDPE-degrading bacterial consortia. The process comprises preparing an active consortium. The active consortium is divided into four parts in centrifuge tubes. The tubes are spun at 5000 rpm. A supernatant is decanted from the tubes. The tubes are vortexed. Talc is added to each tube. The tubes with talc are again vortexed for some time to produce a homogeneous mixture. The mixture is poured into glass dishes. The dishes are kept at room temperature as aseptically for drying.

Abstract: A novel compound 5,8a-di-1-propyl 1-octahydronapthalen-1-(2H) One, and a process for isolating the compound and provides the process for isolation of the compound 5,8a-di-1-propyl 1-octahydronapthalen-1-(2H) One from Trachyspermum ammi (Ajowan caraway) seeds involving washing the seeds, drying the same and crushing it to form a powder; subjecting the powder to solvent extraction; isolating the active compound by chromatography; cooling the fractions; and subjecting the fractions to the step of crystallization in pure methanol.

Abstract: A collector for fecal discharge is provided comprising a self-expanding resilient collection component, a housing sheath and a transit component. The collection component has an open proximal and distal end and a lumen connecting them. The component comprises interconnected resilient arms circumscribing its contour, wherein adjacent arms are resiliently biased away from each other for exerting outwardly radial pressure for expanding and anchoring the component to rectal walls upon deployment. The housing sheath comprises a flexible and resilient material overlaying at least one of inner or outer contour of collection component without interfering with or blocking its ends or lumen. The transit component provides a conduit for fecal discharge to migrate from collection component to a receptacle and comprises a flexible, tubular sheath having a first open end connected to a second end by a lumen, wherein the first end engages with the proximal end of the collection component.

Abstract: This invention relates to a technique to enhance the clinical efficiency of radiotherapy and readiosurgery using perturbative beaming and tissue specific radiobiology, comprising, Considering the normal steady beam dose rate, then increasing the temporal stochastic fluctuation of the output intensity of the radiation beam from a medical linear accelerator or cobalt gamma knife beam setup, developing a computer-based methodology by considering the tumor specific radiobiology and magnitude of the tailor made dose rate fluctuation using an embedded system device consisting of requisite hardware and software as described herein.

Abstract: This invention relates to a process for the production of organic formulation of bio pesticide containing Pseudomonas Fluorescens comprising preparation of Mother culture of P. fluorescens and inoculating in King's B broth, at 30±1° C. for 24-36 hours followed by liquid fermentation process in 5-10% pongamia cake aqueous extract, 5-10% neem cake aqueous extract, 0.3-0.5% sugarcane molasses and King's B broth followed by solid fermentation using sterile Pongamia deoiled cake, Neem deoiled cake, wheat Bran.