Abstract: A human insulin-like growth factor (IGF) binding protein stock solution and method of making the same include a non-recombinant human IGF binding protein-3 (nr-IGFBP-3) in an aqueous buffered medium. The concentration of the nr-IGFBP-3 in the stock solution ranges from about 16 micrograms per milliliter (?g/ml) to about 40 ?g/ml. A kit includes a set of calibrators for nr-IGFBP-3. The set of calibrators includes the nr-IGFBP-3 in different concentrations.

Abstract: Disclosed herein are antibodies that specifically bind to a methotrexate analyte. Such antibodies can be used to detect a methotrexate analyte in a sample, such as in a homogeneous enzyme immunoassay method. Hapten structures to elicit such antibodies and conjugates useful in immunoassays are also described.

Abstract: A method to assess a subject diagnosis is provided. The method includes receiving an image from an image-capturing device, the image comprising an area of interest in a test cartridge, and finding a border of the area of interest of the test cartridge and applying a geometrical transformation on an area delimited by the border of the test cartridge to bring the image of the area of interest in the test cartridge to a selected size and a selected shape. The method also includes identifying a target region within the area of interest of the test cartridge, evaluating a quality of the image based on a characteristic feature of the target region, and providing commands to adjust an optical coupling in the image-capturing device when the quality of the image is lower than a selected threshold.

Abstract: An apparatus is provided. A strip has first and second end sections, and a first face and second face. Two electrocardiographic electrodes are provided on the strip with one of the electrocardiographic electrodes provided on the first face of the first end section of the strip and another of the electrocardiographic electrodes positioned on the first face on the second end section of the strip. A flexible circuit is mounted to the second face of the strip and includes a circuit trace electrically coupled to each of the electrocardiographic electrodes. The apparatus includes a wireless transceiver and a battery.

Abstract: A fluidic tubing assembly and method for a blood analyzer comprising a base, a first tube, and a second tube. The base is connectable to the blood analyzer and has a front side, a rear side, a first side, a second side, a top side, and a bottom side. A first connector is supported by the first side. The first end of the first tube is connected to the first connector. A second connector is supported by the top side. The second end of the first tube is connected to the second connector. A third connector is supported by the top side. The first end of the second tube is connected to the third connector. A fourth connector is supported by the bottom side. The second end of the second tube is connected to the fourth connector.

Abstract: There is provided a chloride selective membrane including an epoxide-based matrix reacted with a stoichiometric amount of an amino compound and an activator such that the epoxide-based matrix comprises a number of quaternary ammonium groups.

Abstract: The timed system for rapid assay is a cartridge system capable of receiving a sample system and performing an assay of the enclosed sample. The cartridge includes a reaction chamber for conducting the assay, with access between the sample system, reaction chamber, and reagent canisters controlled by the motion of a valve. Motion of the valve is in turn controlled by a gearing subsystem that allows for timed opening of various fluent and fluid lines between the sample system, reaction chamber, and reagent canisters.

Abstract: Disclosed is a sensor assembly including a flow channel; two or more working electrodes located in the flow channel; and one or more reference electrodes located in the flow channel, wherein a total number of working electrodes is greater than a total number of reference electrodes. Volume in the flow channel may be minimized Liquid testing apparatus and methods of testing test liquids are provided, as are other aspects.

Abstract: Embodiments provide a fitting for tubing connection, a male fitting assembly, and a torque adaptor. The fitting for tubing connection includes a head including a plurality of first gripping portions, each first gripping portion extending from a top of the head; and a shaft attached to the head, wherein the shaft comprises a plurality of external threads.

Abstract: The invention provides an advanced, modular, intraoperative neurophysiological monitoring (IONM) system, referred to as a “NeuroNet-VII” System, which is the first IONM system designed with a USB hub architecture comprising serially-connected functional modules which provide multi-modality simultaneous data acquisition which support all data types useful in operating rooms, diagnostic laboratories, intensive care units, and epilepsy monitoring units. The unique module architecture makes the IONM system highly modular compared to current systems which typically place components in a limited number of centralized enclosures.

Abstract: A method of monitoring a specimen container or specimen in a diagnostic laboratory system includes moving the specimen container on a track within the diagnostic laboratory system; moving a sensor module on the track; and monitoring at least one characteristic of the specimen container or a specimen located in the specimen container using the sensor module. Other methods, sensor modules, and diagnostic laboratory systems are disclosed.

Abstract: A method for calibrating an imager of a reagent analyzer, comprises positioning a dry reagent pad at a first read position in a field of view of the imager, the first read position illuminated by an illumination source with a first intensity, detecting a reference optical signal by the imager, indicative of a first reflectance value of the dry reagent pad at the first read position, positioning the dry reagent pad at a second read position, the second read position illuminated with a second intensity different from the first intensity, detecting a first optical signal by the imager, indicative of a second reflectance value of the dry reagent pad at the second read position, and calculating, by a processor, a calibration factor for the dry reagent pad at the second read position based on a difference between the reference optical signal and the first optical signal.

Abstract: Kits containing a multiplexed chemiluminescent detection system and microfluidics devices and methods for detecting the presence and/or concentration of biotin and at least one target analyte in a sample are disclosed. The kits, microfluidics devices, and methods utilize singlet oxygen-activatable chemiluminescent compounds in combination with two or more fluorescent molecules that emit light at different wavelengths.

Abstract: Kits containing a chemiluminescent detection system and microfluidics devices and methods for determining the concentration of biotin in a sample are disclosed. The kits, microfluidics devices, and methods utilize a sensitizer capable of generating singlet oxygen in its excited state and having avidin or an analog thereof directly or indirectly bound thereto, as well as a singlet oxygen-activatable chemiluminescent compound having biotin or an analog thereof directly or indirectly bound thereto.

Abstract: An asthma management system and method are disclosed for collecting environmental and individual health data to predict the onset of asthma symptoms to allow for preventative therapy tailored on an individual basis. In one embodiment a computer system is in electrical communication with an individual user interface and one or more environmental factor collection points via a communications network. The user interface is adapted to send and receive asthma-related data including asthma profile and real-time asthma status data to the computer system via the communication network, and the environmental factor collection points are adapted to send and receive data to the computer system via the communications network.

Abstract: A method of characterizing a specimen container or a specimen in an automated diagnostic system includes capturing an image of a specimen container containing a specimen using an imaging device. The method further includes characterizing the image using a first AI model and determining whether a characterization confidence of the image is below a pre-selected threshold. The first AI model is retrained with the image having the characterization confidence below the pre-selected threshold to a second AI model, wherein the retraining includes data selected from one or more of a group of: non-image data, and text data. Quality check modules and systems configured to perform the method are also described, as are other aspects.

Abstract: An apparatus and method for transferring a fluid sample from a fluid sample collection apparatus to a liquid sample analyzer. The apparatus includes a barrel having a first end, a second end, a sidewall extending between the first end and the second end, and an inner surface defining an internal chamber. The first end has an inlet opening and the second end having an outlet opening. The first end of the barrel has a barrel connection portion engageable with a portion of the fluid sample collection apparatus and the second end of the barrel has a tubular portion configured to engage with the liquid sample analyzer so a combination of the fluid sample collection apparatus and the barrel is attachable to the liquid sample analyzer with no additional support.

Abstract: A lysis device including a sample vessel, at least one piezo element, and a controller is disclosed. The sample vessel has a microchannel formed therein. The sample vessel has at least one port extending through a surface to the microchannel. The piezo element is attached to the surface of the sample vessel. The controller has logic to cause the controller to emit a first signal including a series of frequencies to the at least one piezo element to cause the at least one piezo element to generate ultrasonic acoustic standing waves in the sample vessel, to receive a second signal indicative of measured vibration signals from the sample vessel detected by the at least one piezo element, and to determine a resonant frequency of the sample vessel using the measured vibration signals.

Abstract: The present disclosure pertains to techniques that leverage machine learning models to identify tumor-specific mutations through an integrated analysis of next generation sequencing data. In a particular aspect, a computer-implemented method is provided that includes generating sequence reads from one or more samples collected from the same patient, generating variant call files by analyzing the sequence reads corresponding respectively to the one or more samples, comparing variant call files to generate a list of candidate somatic variants, generating, by a classification machine learning model, scores for each of the candidate somatic variants in the list of candidate somatic variants, where the scores are generated based on a plurality of classifications generated by the classification machine learning model, determining, based on the scores, a ctDNA status for the patient, where the ctDNA status is either positive or negative, and generating a report that provides the ctDNA status for the patient.