Abstract: An antigen with an increased half-life is provided for the formulation of more stable and consistent clinical diagnostic immunoassay controls and calibrators. An antigen analogue comprises a first and a second polypeptide which is identical or similar to corresponding terminal amino acid sequences of an antigen. The first and second polypeptides are connected with a PEG chain. Also provided are methods of calibrating assays using a compound disclosed herein.
Type:
Grant
Filed:
June 14, 2022
Date of Patent:
October 22, 2024
Assignee:
Ortho Clinical Diagnostics, Inc.
Inventors:
Brett Hirsch, Jian Zheng, Stephen Daggett
Abstract: A computer-implemented method for detecting a failure of a device connected to a sensor is disclosed. The method includes a machine learning model receiving a trace signal from the sensor indicating a status of the device, the machine learning model encoding the trace signal into a plurality of vector representations, and the machine learning model determining whether the trace signal is valid or invalid based on the plurality of vector representations.
Type:
Grant
Filed:
February 25, 2021
Date of Patent:
October 22, 2024
Assignee:
Siemens Healthcare Diagnostics Inc.
Inventors:
Mark Edwards, Vivek Singh, Bogdan Georgescu, Ankur Kapoor
Abstract: An apparatus and method for removing bubbles from a fluid sample. The apparatus has a barrel and a filter member. The barrel has a first end, a second end, a sidewall, and an inner surface defining an internal chamber. The first side has an inlet opening and the second side has an outlet opening. The filter member is slidably disposed within the internal chamber and defines an inlet side and an outlet side of the internal chamber. The filter member has a gas-permeable, liquid-impermeable membrane which permits the gas portion of the fluid sample to pass across the filter member. The barrel has a second gas-permeable, liquid-impermeable membrane which provides a fluid-tight seal across the outlet opening.
Abstract: An apparatus, vortex generator assembly and method for automated cell lysis and nucleic acid purification and processing. The vortex generator assembly includes sample holder having a lysis well, at least one wash well, and an elution well. The vortex generator assembly also includes a sample holder cover having a plurality of vibration rods for creating a vortex in the wells of the sample holder. The apparatus includes motor operating a rotating cam to cause the vibration rods to vibrate and create the vortex in a well holding fluid and magnetic beads, wherein the vortexing speed is sufficient to overcome the magnetic attraction between the beads and disperse the beads in solution, to collect nucleic acids such as DNA.
Abstract: An apparatus and method for removing bubbles from a fluid sample. The apparatus has a barrel and a filter member. The barrel has a first end, a second end, a sidewall, and an inner surface defining an internal chamber. The first side has an inlet opening and the second side has an outlet opening. The filter member is disposed within the internal chamber and defines an inlet side and an outlet side of the internal chamber. The filter member has a gas-permeable, liquid-impermeable membrane which permits the gas portion of the fluid sample to pass across the filter member from the inlet side to the outlet side. The gas-permeable, liquid-impermeable membrane provides a fluid-tight seal across the filter member. The filter member is pierceable so a probe may be passed through the filter member to withdraw the liquid portion of the fluid sample from the internal chamber.
Abstract: Methods of real-time detection of short-sample aspiration faults in an automated diagnostic analysis system include spectral analysis of a pressure slope waveform based on aspiration pressure measurement signals. The spectral analysis may include using a moving average filter or a wavelet transform, such as, e.g., a continuous wavelet transform (CWT) or a discrete wavelet transform (DWT), to identify distinct transient behavior in the pressure slope waveform. These methods accurately identify short-sample aspiration faults such that an automated diagnostic analysis system can timely terminate an analysis of a detected short sample to avoid a possibly erroneous sample test result. Apparatus for real-time detection of short-sample aspiration faults is also provided, as are other aspects.
Abstract: Embodiments provide a tube retainer system, including: an outer body; a diaphragm having a circular opening; a valve including a valve head and a valve stem attached to the valve head; a cam; a camshaft extending through the cam; a drive gear attached to the camshaft; a restraint gear attached to the camshaft; a push bar located below the diaphragm; and a pawl. The diaphragm, the cam, the drive gear, the restraint gear, the push bar, and the pawl are all provided in the outer body, when a bottom of a tube is inserted into the circular opening and a downward pressure is applied on the diaphragm, the cam is oriented to close the valve to form a partial vacuum in the outer body to secure the bottom of the tube within the circular opening.
Abstract: A processing unit for automated preparation of biological samples included in specimen tubes is described herein. The processing unit includes a removable sample preparation component configured to receive specimen tubes, read identifiers associated with the biological samples contained in the specimen tubes, mix reagents included in the specimen tubes, and transfer the specimen tubes to at least one of a centrifuge or a storage compartment. The processing unit further includes a centrifuge loader for automatic loading and unloading of the specimen tubes onto a centrifuge. The automated centrifuge loader includes a magnetic brake that can stop the centrifuge rotor at a precise position to facilitate loading and unloading of a specimen tube. The centrifuge includes a swing bucket designed to pivot around an axis and slide vertically to enable moving the bucket to a position in which the swing bucket can engage with the port where the specimen tube resides.
Type:
Application
Filed:
June 18, 2024
Publication date:
October 10, 2024
Applicant:
Babson Diagnostics, Inc.
Inventors:
Mark S. FLANAGAN, James PAOLINO, David George SORENSON, Nicholas Robert MONACO
Abstract: Improved analyte detection system within a blood gas analyzer, the improved system comprising and/or consisting of at least one CO-oximetry system that is formed as an integrated, unitary structure(s) with an electrochemical sensor module, and methods of use related thereto.
Abstract: Methods and reagents are disclosed for minimizing a false result in an assay measurement for determining a concentration of an analyte in a sample suspected of containing the analyte. The method comprises pretreating both an antibody and a sample to be subjected to a non-agglutination immunoassay. In the method the antibody and the sample are combined with a pretreatment agent selected from the group consisting of hydroxyphenyl-substituted C1-C5 carboxylic acids and metallic salts thereof and halogen-substituted C1-C5 carboxylic acids and metallic salts thereof in an amount effective to enhance the accuracy of the non-agglutination immunoassay.
Abstract: Methods of early real-time detection or prediction of aspiration faults in an automated diagnostic analysis system include an artificial intelligence algorithm configured to use either cluster analysis or probabilistic graphical modeling based on an aspiration pressure measurement signal waveform. Aspiration faults may include short-volume aspiration and unwanted gel pick-up. These methods may allow for timely termination of an aspiration process so as to avoid or minimize possible detrimental downstream consequences such as faulty sample test results and/or instrument downtime for servicing and cleanup. Apparatus for early real-time detection or prediction of aspiration faults are provided as are other aspects.
Abstract: Reagents are disclosed for use with potentiometric magnesium ion selective electrodes, along with kits containing same as well as methods of use thereof.
Abstract: The present disclosure provides methods and kits for direct sequencing of nucleic acid targets. Such methods can be used to determine if one or more nucleic acid targets are present in a sample.
Abstract: A machine learning system and method for somatic mutation discovery are provided that provides improved identification of tumor-specific mutations. The improved identification of tumor-specific mutations may affect discovery of alterations and therapeutic management of cancer patients.
Abstract: An analyte detection apparatus is disclosed and configured for testing a substance for the presence or absence of at least one target analyte. In at least one embodiment, a housing provides a mixing chamber and a testing chamber in selective fluid communication with the mixing chamber. During use of the apparatus, an end cap is disengaged from the housing, a volume of the substance is collected via a collection scoop provided by the end cap, the end cap is re-engaged with the housing, thereby introducing the substance from the collection scoop into the mixing chamber, the substance is mixed with a delivery fluid within the mixing chamber, and a mixing valve is rotated into an open position, allowing the substance to travel into the testing chamber and come into contact with a test medium, with a resulting visual indication from the test medium being viewable via a result window.
Type:
Application
Filed:
March 21, 2024
Publication date:
September 26, 2024
Applicant:
Defense Diagnostics Inc.
Inventors:
Ahmad Hussain, Jeremy Ridley, Chris Kolb, Justin Bechstein, Ayan Bhandari, Stefan Grady Foulstone, Rose Olivieri
Abstract: Methods of characterizing a sample container or a biological sample in an automated diagnostic analysis system using an artificial intelligence (AI) algorithm include retraining of the AI algorithm in response to characterization confidence levels determined to be unsatisfactory. The AI algorithm is retrained with data (including image data and/or non-image data) having features prevalent at the site where the automated diagnostic analysis system is operated, which were not sufficiently or at all included in training data used to initially train the AI algorithm. Systems for characterizing a sample container or a biological sample using an AI algorithm are also provided, as are other aspects.
Type:
Application
Filed:
July 6, 2022
Publication date:
September 26, 2024
Applicant:
Siemens Healthcare Diagnostics Inc.
Inventors:
Venkatesh NarasimhaMurthy, Vivek Singh, Yao-Jen Chang, Benjamin S. Pollack, Ankur Kapoor, Rayal Raj Prasad Nalam Venkat
Abstract: A method for detecting an abnormal operating condition of a heat exchanger is provided. The method includes determining the actual current through a heat exchanger, using the actual current to indicate an abnormal operating condition, and declaring a fault state. A step of comparing the baseline current to the actual current includes normalizing the actual current, calculating a difference between the normalized actual current and the baseline current, and determining that difference is less than zero.
Abstract: Kits containing a multiplexed chemiluminescent detection system and microfluidics devices and methods for detecting the presence and/or concentration of biotin and at least one target analyte in a sample are disclosed. The kits, microfluidics devices, and methods utilize singlet oxygen-activatable chemiluminescent compounds in combination with two or more fluorescent molecules that emit light at different wavelengths.