Abstract: A cell observation system according to the present invention includes: a first image-acquisition device disposed in an incubator and acquires a first image of cells in a culturing vessel; a second image-acquisition device disposed outside the incubator; a processing device connected to the first image-acquisition device and the second image-acquisition device; and a display displays at least a region of the first image, as well as the second image. The second image-acquisition device includes a second image-acquisition unit that acquires a second image of the interior of the culturing vessel, that removed from the incubator. The processing device extracts target cells in the first image, calculates positions at which the extracted target cells are present and stores the positions in the memory, and causes the display to display the positions at which the target cells are present in a superimposed manner on an indication indicating the culturing vessel.

Abstract: A cell observation system according to the present invention includes: a first image-acquisition device disposed in an incubator and acquires a first image of cells in a culturing vessel; a second image-acquisition device disposed outside the incubator; a processing device connected to the first and second image-acquisition devices; and a display. The second image-acquisition device includes a second image-acquisition unit that acquires a second image of the interior of the culturing vessel that removed from the incubator, a support that supports the second image-acquisition unit and the culturing vessel, and a position measuring unit that measures a position between the culturing vessel and the second image-acquisition unit at the time of acquiring the second image. The processing device extracts target cells in the first image, calculates positions of the target cells, and displays the relationship between the positions of the target cells and the second image.

Abstract: An observation device includes: an illumination optical system configured to emit illumination light to above a sample from below; and an objective optical system configured to acquire an image of transmitted light below the sample in a path different from a path for the illumination optical system, the transmitted light resulting from the illumination light that has been emitted from the illumination optical system, that has been reflected above the sample, and that has passed through the sample. The objective optical system includes, in the vicinity of a pupil plane, a pupil modulation element that partially decreases transmittance and that modulates the phase of light, and the illumination optical system includes a light source, and an illumination-region restricting section configured to restrict light from the light source to a particular emission region.