Patents Assigned to Gesellschaft fur Biotechnologische Forschung
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Patent number: 8513280Abstract: The present invention is about the use of at least one epothilone or derivative thereof as an active ingredient for manufacturing a medicament for use in the treatment of disease(s) involving a neuronal connectivity defect.Type: GrantFiled: September 9, 2010Date of Patent: August 20, 2013Assignees: Institut National de la Sante et de la Recherche Medicale, Commissariat a l'Energie Atomique, Gesellschaft fur Biotechnologische Forschung mbH (GBF)Inventors: Annie Andrieux, Didier Job, Annie Schweitzer, Gerhard Höfle
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Publication number: 20080120733Abstract: The invention relates to a method for the production of a eukaryotic cell selectable by inactivation or reduction of an endogenous gene function, comprising the steps of (a) introduction of one or more vectors into the cell and (b) expression of a siRNA and preferably shRNA coded by the one or more vectors, directed against an endogenous selectable gene and inactivating same, said siRNA or shRNA being the transcription product of an RNAi selection cassette, the selection cassette comprising a section of at least 19 nucleotides of the transcribed region of the gene, said selection being operatively linked to a promoter and a transcription termination signal.Type: ApplicationFiled: June 16, 2005Publication date: May 22, 2008Applicant: GBF-GESELLSCHAFT FUR BIOTECHNOLOGISCHE FORSCHUNGInventors: Martin Hafner, Karina Nawrath, Werner Muller
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Patent number: 7316996Abstract: The invention provides lipopeptide conjugates in which a cysteine that is double-substituted by a fatty acid is bonded by means of the carboxyl group to a highly soluble, physiologically compatible and non-immunogenic, polymeric conjugate group. The conjugates exhibit an excellent macrophage stimulant action and do not require addition solutizing. They can be used in a wide range of applications, in particular for stimulating macrophages, for stimulating antibody synthesis, for combating infection, as an immunostimulant, in particular in relation to tumors, for preventing and treating septicaemic shock, for would healing and as an adjuvant for vaccines.Type: GrantFiled: July 18, 2003Date of Patent: January 8, 2008Assignee: GBF Gesellschaft fur Biotechnologische Forschung mbHInventors: Peter Muhlradt, Michael Morr
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Patent number: 6992235Abstract: The invention relates to a method for marker-free DNA expression cassette exchange in the genome of cells or parts of cells by using the FLP recombinase mediated cassette exchange. A first DNA expression cassette carrying a positive-negative selection marker flanked by two FLP recombinase recognition target (FRT) sites is integrated into a chromosomal locus of the genome. Following selection of cell clones surviving the conditions for positive selection, the first DNA cassette is exchanged by an incoming second DNA expression cassette located on a circular vector and carrying a transgene flanked by the same FRT sites as the first DNA cassette by using FLP-recombinase. The cell clones surviving the conditions for negative selection contain specifically inserted the gene of the incoming DNA cassette without inserted unwanted vector sequences or positive selectable markers.Type: GrantFiled: April 25, 2001Date of Patent: January 31, 2006Assignee: Gesellschaft fur Biotechnologische Forschung mbHInventors: Jürgen Bode, Jost Seibler, Dirk Schübeler
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Patent number: 6831076Abstract: The present invention relates to epothilon derivatives and to their use.Type: GrantFiled: June 25, 2003Date of Patent: December 14, 2004Assignee: Gesellschaft fur Biotechnologische Forschung mbH (GBF)Inventors: Gerhard Hoefle, Michael Kiffe
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Patent number: 6613912Abstract: The present invention relates to epothilon derivatives and to their use.Type: GrantFiled: April 16, 2001Date of Patent: September 2, 2003Assignee: Gesellschaft fur Biotechnologische Forschung mbH (GBF)Inventors: Gerhard Hoefle, Michael Kiffe
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Patent number: 6288237Abstract: The present invention relates to epothilon derivatives and to their use.Type: GrantFiled: August 3, 1998Date of Patent: September 11, 2001Assignee: Gesellschaft fur Biotechnologische Forschung mbH (GBF)Inventors: Gerhard Hoefle, Michael Kiffe
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Patent number: 6060273Abstract: Multicistronic expression units allow the equimolar expression of the genes located in the corresponding cistrons. These expression units are particularly suitable for the recombinant production of proteins composed of two or more polypeptide subunits.Type: GrantFiled: June 2, 1997Date of Patent: May 9, 2000Assignees: Beiersdorf AG, Gesellschaft fur Biotechnologische Forschung mbHInventors: Wilhelm Dirks, Manfred Wirth, Hansjorg Hauser, Wolfram Eichner, Volker Achterberg, Albrecht Dorschner, Wolfgang Meyer-Ingold, Heiko Mielke
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Patent number: 5925559Abstract: The invention concerns a collection of phagemids made up of a promotor, a gene of a fusion between a ligand protein, wherein the ligand protein in its processed state would be of from 40 to 60 amino acid residues, and a bacteriophage protein, at least one transcription terminator sequence, a replication origin derived from a single strand bacteriophage, a plasmid replication origin and facultatively at least one selection marker. Further, the invention concerns a collection of Escherichia coli clones or cells representing the collection of phagemids, a process for isolating phagemid particles with strong binding characteristics for a defined target molecule with the use of collections according to the invention and phagemid particles obtained according to the process.Type: GrantFiled: June 23, 1997Date of Patent: July 20, 1999Assignee: Gesellschaft fur Biotechnologische Forschung GmbHInventors: John Collins, Peter Roettgen
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Patent number: 5830637Abstract: A single, unitary, solid phase support apparatus having a planar surface divided into a plurality of separate zone functions to detect antibodies. Each zone has bonded to it, a different peptide through its C-terminal end. The zones are incubated with the analyte sample and observed for reaction, indicating the virus-specific or bacteria specific presence or absence.Type: GrantFiled: September 30, 1996Date of Patent: November 3, 1998Assignee: Gesellschaft fur Biotechnologische Forschung mbH (GBF)Inventors: Ronald Frank, Sinan Guler
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Patent number: 5770439Abstract: A biosensor is produced by depositing thick film pastes by screen-printing on a substrate to produce thick-film conductivity electrodes, and immobilizing enzymes on the electrodes. In specific embodiments, the substrate is an unfired ceramic, a fired ceramic or a plastic, the paste is a noble metal paste, enzyme immobilization is with a bifunctional reagent, a plurality of different enzymes are immobilized on different electrodes, and/or the electrodes are printed as parallel lines or in the image of a comb or concentric circles. A multi-dimensional biosensor is prepared by forming thick film paste electrodes containing an immobilized enzyme on a plurality of separate substrates, disposing the separate substrates one on top of the other in a vertical stack, pressing the stacked substrates together and curing the paste.Type: GrantFiled: September 28, 1992Date of Patent: June 23, 1998Assignee: Gesellschaft fur Biotechnologische Forschung mbB (GBF)Inventors: Ursula Bilitewski, Wiebke Drewes, Franz Bechtold
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Patent number: 5763227Abstract: The invention relates to a process for the production of a primer for consecutive DNA sequencing, in which at least two shortmers are hybridised adjacent to one another using a template and are then ligated with one another to form the primer, the process being characterised in that(a) a template in the form of a single strand of nucleotides is used in which the number of nucleotides is at least 6 and is not greater than approximately twice the total number of nucleotides of all the shortmers used, and(b) the primer formed is then separated from the template and from unreacted shortmers and recovered. The invention relates also to banks (libraries) of templates for the mentioned process.Type: GrantFiled: April 29, 1996Date of Patent: June 9, 1998Assignee: Gesellschaft fur Biotechnologische Forschung mbH (GBF)Inventor: Helmut Blocker
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Patent number: 5756758Abstract: The invention relates to compounds which can be reacted with a carboxyl function with formation of an ester bond, whereby a protective group and, in particular, an anchor group for the carboxyl function is provided. The invention further relates to esters of acids which are obtainable by esterification with a compound according to the invention, and to the use of the compounds according to the invention in peptide synthesis.Type: GrantFiled: December 7, 1995Date of Patent: May 26, 1998Assignee: Gesellschaft fur Biotechnologische Forschung mbH IGBF)Inventors: Ronald Frank, Stefan Hoffmann
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Patent number: 5753256Abstract: The invention relates to a plaster for the treatment of nail mycoses, which s characterized by an antimycotically active substance, clotrimazole and bifonazole being excluded.Type: GrantFiled: May 6, 1996Date of Patent: May 19, 1998Assignee: Labtec Gesellschaft fur Biotechnologische Forschung und Entwicklung mbHInventors: Gunter Cordes, Ulrike Vollmer
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Patent number: 5705361Abstract: The invention relates to a hybrid transposon having a PT operon, to plasmids and to bacterial strains (hosts) therefor.Type: GrantFiled: October 21, 1993Date of Patent: January 6, 1998Assignee: Gesellschaft fur Biotechnologische Forschung mbH GBFInventors: Mark Walker, Kenneth Timmis
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Patent number: 5665567Abstract: The recombinant production of PDGF-AB in mammalian cells is disclosed by means of a bicistronic vector system in which an IRES sequence is located between the first and second cistrons and in which the B chain coding gene is located in the first cistron. The disclosed expression unit allows the equimolor expression of the A and B polypeptide chains.Type: GrantFiled: April 24, 1995Date of Patent: September 9, 1997Assignees: Beiersdorf AG, Gesellschaft fur Biotechnologische Forschung mbHInventors: Wolfram Eichner, Volker Achterberg, Albrecht Dorschner, Wolfgang Meyer-Ingold, Heiko Mielke, Wilhem Dirks, Manfred Wirth, Hansjorg Hauser
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Patent number: 5622979Abstract: The invention relates to compounds of the following general formula I ##STR1## especially the compound of formula Ia referred to as thiangazole ##STR2## their pharmaceutically acceptable acid addition salts, processes for their preparation and therapeutic compositions and compositions that can be used in crop-protection.Type: GrantFiled: June 7, 1995Date of Patent: April 22, 1997Assignees: Ciba-Geigy Corporation, Gesellschaft fur Biotechnologische Forschung mbHInventors: Gerhard H ofle, Norbert Bedorf, Edgar Forche, Klaus Gerth, Herbert Irschik, Rolf Jansen, Brigitte Kunze, Hans Reichenbach, Florenz Sasse, Heinrich Steinmetz, Wolfram Trowitzsch-Kienast, Johannes P. Pachlatko
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Patent number: 5610038Abstract: The invention relates to compounds of the following general formula I ##STR1## especially the compound of formula Ia referred to as thiangazole ##STR2## their pharmaceutically acceptable acid addition salts, processes for their preparation and therapeutic compositions and compositions that can be used in crop-protection.Type: GrantFiled: June 7, 1995Date of Patent: March 11, 1997Assignees: Ciba-Geigy Corporation, Gesellschaft fur Biotechnologische Forschung mbHInventors: Gerhard Hofle, Norbert Bedorf, Edgar Forche, Klaus Gerth, Herbert Irschik, Rolf Jansen, Brigitte Kunze, Hans Reichenbach, Florenz Sasse, Heinrich Steinmetz, Wolfram Trowitzsch-Kienast, Johannes P. Pachlatko
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Patent number: 5604249Abstract: The invention relates to compounds of the following general formula I ##STR1## especially the compound of formula Ia referred to as thiangazole ##STR2## their pharmaceutically acceptable acid addition salts, processes for their preparation and therapeutic compositions and compositions that can be used in crop-protection.Type: GrantFiled: June 7, 1995Date of Patent: February 18, 1997Assignees: Ciba-Geigy Corporation, Gesellschaft fur Biotechnologische Forschung mbHInventors: Gerhard H ofle, Norbert Bedorf, Edgar Forche, Klaus Gerth, Herbert Irschik, Rolf Jansen, Brigitte Kunze, Hans Reichenbach, Florenz Sasse, Heinrich Steinmetz, Wolfram Trowitzsch-Kienast, Johannes P. Pachlatko
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Patent number: 5480790Abstract: A water-soluble protein conjugate modified by saccharides is covalently bound to a carbohydrate backbone via a saccharide group. Such protein conjugates modified by saccharides can be produced in a simple way by polymerizing a vinylsaccharide and conjugating in a known way the poly(vinylsaccharide) obtained in this way with the protein, which is preferably an enzyme. The protein conjugates according to the present invention are stable over long time periods at a high enzymatic activity and also in aqueous solution and are thus particularly suitable for use in test kits.Type: GrantFiled: April 4, 1991Date of Patent: January 2, 1996Assignees: Boehringer Mannheim GmbH, Gesellschaft fur Biotechnologische Forschung mbHInventors: Wilhelm Tischer, Joachim Klein, Rolf-Joachim Muller, Stephan Engelke