Patents Assigned to HIROSAKI UNIVERSITY
  • Patent number: 10562989
    Abstract: A fluorine-containing boric acid PVB composite comprising a condensate of a fluorine-containing alcohol, boric acid and polyvinyl butyral, wherein the fluorine-containing alcohol is represented by the general formula: Rf(A-OH)k wherein k is 1 or 2; when k is 1, Rf is a perfluoroalkyl group having 6 or less carbon atoms, a polyfluoroalkyl group in which some of the fluorine atom or atoms of the perfluoroalkyl group are replaced by hydrogen atom or atoms, a polyfluoroalkyl group containing a terminal perfluoroalkyl group having 6 or less carbon atoms and a perfluoroalkylene group having 6 or less carbon atoms, or a linear or branched, perfluoroalkyl group containing a terminal perfluoroalkyl group having 6 or less carbon atoms, a perfluoroalkylene group having 6 or less carbon atoms and ether bonds; when k is 2, Rf is a linear or branched, perfluoroalkylene group or polyfluoroalkylene group, containing a perfluoroalkylene group having 6 or less carbon atoms and ether bonds; and A is an alkylene group having
    Type: Grant
    Filed: March 10, 2016
    Date of Patent: February 18, 2020
    Assignees: Unimatec Co., Ltd., Hirosaki University
    Inventors: Takeshi Fukushima, Katsuyuki Sato, Hideo Sawada
  • Patent number: 10551387
    Abstract: A cancer cell detection method that makes it possible to perform imaging of cells in a living state and a dual detection method for cancer cells in which the aforementioned method is combined with a pre-existing dyeing method for cytodiagnosis. The method uses living body-derived cells and includes: incubating living cells included in a sample taken from a person together with a fluorescently-labeled L-glucose derivative and detecting the fluorescently-labeled L-glucose derivative that is taken up into the cells; and detecting fluorescence emitted by the L-glucose derivative that is present within the cells while the cells are attached to a thin glass or plastic plate. Also provided is a dual detection method for cancer cells in which the cancer cell detection method that uses living body-derived cells is combined with a dyeing method using cells that are fixed using an alcohol or the like.
    Type: Grant
    Filed: September 24, 2015
    Date of Patent: February 4, 2020
    Assignee: HIROSAKI UNIVERSITY
    Inventors: Katsuya Yamada, Ayako Sasaki, Kouki Ono, Kiyoshi Tone
  • Patent number: 10509041
    Abstract: Provided is a glucose derivative, which is taken into cells via a membrane sugar transport system and is represented by formula (1). Also provided are an imaging agent and an imaging method for a cell or intracellular molecule using said glucose derivative. Further provided is a method for detecting cancer cells with good accuracy using said glucose derivative and an imaging agent to be used in said method. More specifically provided are D-glucose derivatives and L-glucose derivatives in which glucose is bound to the 7-position of a fluorescent molecular group with a coumarin backbone or a quinolone backbone. Also provided are a cell imaging agent and imaging method using the derivative. A cancer cell imaging agent and imaging method using the L-glucose derivative is also provided. G is a group selected from formulas (G1)-(G4) below.
    Type: Grant
    Filed: April 19, 2018
    Date of Patent: December 17, 2019
    Assignee: HIROSAKI UNIVERSITY
    Inventors: Katsuya Yamada, Ayako Sasaki, Tadashi Teshima, Toshihiro Yamamoto, Yuji Otsuka
  • Patent number: 10504225
    Abstract: An image processing device includes: an acquisition unit that acquires a probability distribution of luminance of a pixel in a plurality of perspective images including the luminance of the pixel which is superposition of luminance components resulting from respective objects, based on an occurrence frequency of a perspective image in which the luminance of the pixel has respective values of a plurality of values; a distribution estimation unit that estimates a mixture distribution indicating the acquired probability distribution; a component estimation unit that estimates a component corresponding to a smallest expectation value distribution of which an expectation value is the smallest among a plurality of partial distributions that form the estimated mixture distribution, and forming at least a portion of the luminance of the pixel; a removal unit that removes the estimated component from the luminance of the pixel included in the perspective image.
    Type: Grant
    Filed: September 29, 2017
    Date of Patent: December 10, 2019
    Assignees: TOHOKU UNIVERSITY, HIROSAKI UNIVERSITY
    Inventors: Noriyasu Homma, Masao Sakai, Kei Ichiji, Naoki Shibusawa, Xiaoyong Zhang, Makoto Abe, Norihiro Sugita, Makoto Yoshizawa, Yoshihiro Takai
  • Patent number: 10302638
    Abstract: An automatic analyzing apparatus 10 includes a chip rack 11 that stores a pipette chip, a pipette 12 into which a specimen is injected, a conveyance unit that conveys the pipette 12 by parallel translation, a reagent rack 14, a reaction unit 15, a detection unit 16, and a detection block unit 17. The pipette chip stored by the chip rack 11 has a planar structure to directly and optically detect the specimen. The chip rack 11 includes, in a hole that receives the pipette chip, a guide corresponding to the structure of the pipette chip. The pipette 12 sucks or discharges the specimen via the pipette chip mounted onto the tip thereof by a drive of a pump. In the detection unit 16, a measurement is carried out with the pipette chip arranged so that the plane that receives light is vertical to an optical axis.
    Type: Grant
    Filed: May 19, 2014
    Date of Patent: May 28, 2019
    Assignees: SYSTEM INSTRUMENTS CO., LTD., HIROSAKI UNIVERSITY, NATIONAL UNIVERSITY CORPORATION KOBE UNIVERSITY
    Inventors: Kazuyuki Hamada, Takeshi Akiba, Chikara Ohyama, Tohru Yoneyama, Yuki Tobisawa, Toshifumi Takeuchi
  • Patent number: 10288604
    Abstract: The present invention has an object of providing a sugar derivative emitting blue fluorescence color which can be used for imaging of cells or intracellular molecules and a method for imaging cells using the derivative. Further, the present invention has an object of providing a method for detecting cancer cells at high accuracy by imaging, and an imaging agent used for this method. The present invention provides a fluorescently labeled sugar derivative having 3-carboxy-6,8-difluoro-7-hydroxycoumarin or 3-carboxymethyl-6,8-difluoro-7-hydroxy-4-methylcoumarin as a fluorescent molecular group in its molecule, and a cell imaging agent and an imaging method using the derivative. Further, the present invention provides an imaging agent and an imaging method for cancer cells using an L-glucose derivative having the above-described fluorescent molecular group in its molecule.
    Type: Grant
    Filed: October 1, 2013
    Date of Patent: May 14, 2019
    Assignee: HIROSAKI UNIVERSITY
    Inventors: Katsuya Yamada, Tadashi Teshima, Toshihiro Yamamoto
  • Publication number: 20190113456
    Abstract: A multi-surface image acquisition system includes a multi-surface observation prism which includes one or more prisms and has a light path formed to collect surfaces in respective directions to be observed of a subject having a three-dimensional structure into one direction, in which a light path length is corrected by utilizing a difference in refractive index between glass and air to equalize a working distance of each surface. A light field camera expands a focal-depth adjustment range for two or more images focused on same plane through the prism. A three-dimensional subject can be observed in multiple directions at the same time, including an observation of cells.
    Type: Application
    Filed: March 30, 2017
    Publication date: April 18, 2019
    Applicant: Hirosaki University
    Inventors: Katsuya Yamada, Kouki Ono, Yasushi Kojima, Terumasa Takamatsu
  • Publication number: 20180328930
    Abstract: An object of the present invention is to provide a novel Pca determination method and a Pca malignancy determination method. The present invention is an invention relating to a “prostate carcinoma determination method, including determining the ratio of the amount of free prostate specific antigen, which has a glycan in which the terminal sialic acid residue of the glycan is ?(2,3)-linked to a second galactose residue from the terminal of the glycan, to the amount of free PSA in a biological sample, and then determining that prostate carcinoma is developed or the probability of developing prostate carcinoma is high in the case where the ratio is 40% or higher.
    Type: Application
    Filed: July 24, 2018
    Publication date: November 15, 2018
    Applicants: FUJIFILM WAKO PURE CHEMICAL CORPORATION, HIROSAKI UNIVERSITY
    Inventors: Chikara OYAMA, Tohru YONEYAMA, Yuki TOBISAWA, Tomokazu ISHIKAWA, Tatsuo KUROSAWA, Kenji NAKAMURA
  • Patent number: 10093812
    Abstract: A composite material of resin—fluorine-containing boric acid composite particles comprising a resin, and a condensate of boric acid and a fluorine-containing alcohol represented by the general formula RF-A-OH (wherein RF is a perfluoroalkyl group having 6 or less carbon atoms, or a polyfluoroalkyl group, in which some of the fluorine atom or atoms of the perfluoroalkyl group are replaced by a hydrogen atom or hydrogen atoms, and which contains a terminal perfluoroalkyl group having 6 or less carbon atoms and a perfluoroalkylene group having 6 or less carbon atoms; and A is an alkylene group having 1 to 6 carbon atoms, or comprising a resin, and a condensate of boric acid, the said fluorine-containing alcohol and an alkoxysilane at a molar ratio of 1.0 or less based on the fluorine-containing alcohol. These composite materials of resin—fluorine-containing boric acid composite particles has good adhesion to inorganic substrates, and the like.
    Type: Grant
    Filed: March 10, 2015
    Date of Patent: October 9, 2018
    Assignees: Unimatec Co., Ltd., Hirosaki University
    Inventors: Takeshi Fukushima, Katsuyuki Sato, Hideo Sawada
  • Patent number: 10088396
    Abstract: Fluorine-containing boric acid composite capsule particles comprising a condensate of a fluorine-containing alcohol, a guest compound, and boric acid particles, wherein the fluorine-containing alcohol is represented by the general formula: RF-A-OH wherein RF is: a perfluoroalkyl group having 6 or less carbon atoms, a linear or branched perfluoroalkyl group containing a terminal perfluoroalkyl group having 6 or less carbon atoms and a perfluoroalkylene group having 6 or less carbon atoms, and containing an O, S, or N atom, or a polyfluoroalkyl group in which some of the fluorine atom or atoms of the perfluoroalkyl group are replaced by hydrogen atom or atoms, and which contains a terminal perfluoroalkyl group having 6 or less carbon atoms and a perfluoroalkylene group having 6 or less carbon atoms, wherein the perfluoroalkylene group may contain an O, S, or N atom, and one fluorine atom of the terminal perfluoroalkyl group may be replaced by —(CH2)fOH (wherein f is an integer of 1 to 3); and A is an alkyl
    Type: Grant
    Filed: March 10, 2015
    Date of Patent: October 2, 2018
    Assignees: Unimatec Co., Ltd., Hirosaki University
    Inventors: Takeshi Fukushima, Katsuyuki Sato, Hideo Sawada
  • Publication number: 20180238899
    Abstract: Provided is a glucose derivative, which is taken into cells via a membrane sugar transport system and is represented by formula (1). Also provided are an imaging agent and an imaging method for a cell or intracellular molecule using said glucose derivative. Further provided is a method for detecting cancer cells with good accuracy using said glucose derivative and an imaging agent to be used in said method. More specifically provided are D-glucose derivatives and L-glucose derivatives in which glucose is bound to the 7-position of a fluorescent molecular group with a coumarin backbone or a quinolone backbone. Also provided are a cell imaging agent and imaging method using the derivative. A cancer cell imaging agent and imaging method using the L-glucose derivative is also provided. G is a group selected from formulas (G1)-(G4) below.
    Type: Application
    Filed: April 19, 2018
    Publication date: August 23, 2018
    Applicant: HIROSAKI UNIVERSITY
    Inventors: Katsuya YAMADA, Ayako SASAKI, Tadashi TESHIMA, Toshihiro YAMAMOTO, Yuji OTSUKA
  • Patent number: 10001487
    Abstract: Provided is a glucose derivative, which is taken into cells via a membrane sugar transport system and is represented by formula (1). Also provided are an imaging agent and an imaging method for a cell or intracellular molecule using said glucose derivative. Further provided are a method for detecting cancer cells with good accuracy using said glucose derivative and an imaging agent to be used in said method. More specifically provided are D-glucose derivatives and L-glucose derivatives in which glucose is bound to the 7-position of a fluorescent molecular group with a coumarin backbone or a quinolone backbone. Also provided are a cell imaging agent and imaging method using the derivative. A cancer cell imaging agent and imaging method using the L-glucose derivative is also provided. G is a group selected from formulas (G1)-(G4) below.
    Type: Grant
    Filed: April 6, 2015
    Date of Patent: June 19, 2018
    Assignee: HIROSAKI UNIVERSITY
    Inventors: Katsuya Yamada, Ayako Sasaki, Tadashi Teshima, Toshihiro Yamamoto, Yuji Otsuka
  • Patent number: 9958450
    Abstract: An object of the present invention is to provide a method for accurately evaluating the specific incorporation of D-glucose into cells. The present invention as a means for achieving the object is characterized by comprising contacting a D-glucose derivative that has a fluorescent chromophore in the molecule and is specifically incorporated into cells and an L-glucose derivative that has a fluorescent chromophore in the molecule with different cells in the same cell strain to be evaluated, respectively, comparing the fluorescence emitted by the D-glucose derivative that has a fluorescent chromophore in the molecule and is specifically incorporated into cells with the fluorescence emitted by the L-glucose derivative that has a fluorescent chromophore in the molecule, and evaluating the specific incorporation of D-glucose into cells relative to L-glucose by taking the difference between the two kinds of fluorescence intensities.
    Type: Grant
    Filed: February 5, 2015
    Date of Patent: May 1, 2018
    Assignee: HIROSAKI UNIVERSITY
    Inventors: Katsuya Yamada, Tadashi Teshima, Toshihiro Yamamoto
  • Patent number: 9933430
    Abstract: The method for distinguishing between prostate carcinoma and benign prostatic hyperplasia comprising: bringing an analyte sample containing a prostate-specific antigen (PSA) into contact with a carrier having an anti-free PSA antibody immobilized thereon, thereby binding free PSA to the anti-free PSA antibody immobilized on the carrier; thereafter bringing the carrier in which the free PSA is bound to the immobilized anti-free PSA antibody into contact with a monoclonal antibody capable of specifically recognizing a glycan in which a terminal sialic acid residue is bound to galactose through an ?(2,3) bond, thereby binding the monoclonal antibody recognizing the glycan to the free PSA bound to the anti-free PSA antibody; measuring the amount of the free PSA having an N-type glycan in which a terminal sialic acid residue is bound to galactose through an ?(2,3) bond; comparing the measured amount thus obtained with a preset cutoff value for prostate carcinoma and benign prostatic hyperplasia, thereby determinin
    Type: Grant
    Filed: October 9, 2013
    Date of Patent: April 3, 2018
    Assignees: HIROSAKI UNIVERSITY, SHIZUOKA PREFECTURAL UNIVERSITY CORPORATION
    Inventors: Chikara Ohyama, Tohru Yoneyama, Yuki Tobisawa, Shingo Hatakeyama, Takashi Suzuki, Ilpal Jwa, Maho Yamaguchi
  • Patent number: 9885005
    Abstract: [Problem] To provide a phospholipid ?-linolenic acid composition of consistent quality and having a stable supply of source material, with focus on Torula yeast, which is edible and recognized as safe, in order to resolve issues with omega 3 sources. [Means for Solving the Problem] As a result of intensive study to resolve the above-noted issues, the inventors of the present invention have discovered that a phospholipid ?-linolenic acid composition can be obtained using residue of yeast extract extracted from Torula yeast (Candida utilis), for example, which is currently produced in large quantities for application in foods, and so arrived at the present invention.
    Type: Grant
    Filed: March 9, 2015
    Date of Patent: February 6, 2018
    Assignees: KOHJIN LIFE SCIENCES CO., LTD., HIROSAKI UNIVERSITY
    Inventors: Naoto Kaji, Shiroh Aida, Katsuhiro Osaki, Masato Omae, Tomohiro Nakagawa, Motoyuki Tokuriki, Hayato Maeda
  • Publication number: 20180025493
    Abstract: An image processing device includes: an acquisition unit that acquires a probability distribution of luminance of a pixel in a plurality of perspective images including the luminance of the pixel which is superposition of luminance components resulting from respective objects, based on an occurrence frequency of a perspective image in which the luminance of the pixel has respective values of a plurality of values; a distribution estimation unit that estimates a mixture distribution indicating the acquired probability distribution; a component estimation unit that estimates a component corresponding to a smallest expectation value distribution of which an expectation value is the smallest among a plurality of partial distributions that form the estimated mixture distribution, and forming at least a portion of the luminance of the pixel; a removal unit that removes the estimated component from the luminance of the pixel included in the perspective image.
    Type: Application
    Filed: September 29, 2017
    Publication date: January 25, 2018
    Applicants: TOHOKU UNIVERSITY, HIROSAKI UNIVERSITY
    Inventors: Noriyasu HOMMA, Masao SAKAI, Kei ICHIJI, Naoki SHIBUSAWA, Xiaoyong ZHANG, Makoto ABE, Norihiro SUGITA, Makoto YOSHIZAWA, Yoshihiro TAKAI
  • Publication number: 20170315129
    Abstract: The purpose of the present invention is to provide a novel cancer cell detection method that uses living body-derived cells and that can be used even in cytodiagnosis. In particular, the purpose of the present invention is to provide a novel cancer cell detection method that makes it possible to perform imaging of cells in a living state and a dual detection method for cancer cells in which the aforementioned method is combined with a pre-existing dyeing method for cytodiagnosis. Provided is a cancer cell detection method that uses living body-derived cells and that includes: incubating living cells included in a sample taken from a person together with a fluorescently-labeled L-glucose derivative and detecting the fluorescently-labeled L-glucose derivative that is taken up into the cells; and detecting fluorescence emitted by the L-glucose derivative that is present within the cells while the cells are attached to a thin glass or plastic plate.
    Type: Application
    Filed: September 24, 2015
    Publication date: November 2, 2017
    Applicant: Hirosaki University
    Inventors: Katsuya YAMADA, Ayako SASAKI, Kouki ONO, Kiyoshi TONE
  • Patent number: 9803044
    Abstract: Disclosed is a fluorine-containing oligomer comprising a copolymer of a fluoroalkyl alcohol (meth)acrylic acid derivative represented by the general formula: CnF2n+1(CH2CF2)a(CF2CF2)b(CH2CH2)cOCOCR?CH2??[I] wherein R is a hydrogen atom or a methyl group, n is an integer of 1 to 6, a is an integer of 1 to 4; b is an integer of 0 to 3; and c is an integer of 1 to 3; and a (meth)acrylic acid derivative represented by the general formula: (CH2?CRCO)mR???[II] wherein R is a hydrogen atom or a methyl group, m is 1, 2, or 3; and when m is 1, R? is OH group, NH2 group that is unsubstituted or mono- or di-substituted with an alkyl group having 1 to 6 carbon atoms, or a monovalent group derived from an alkylene glycol or polyalkylene glycol group containing an alkylene group having 2 or 3 carbon atoms; when m is 2 or 3, R? is a divalent or trivalent organic group derived from a diol or triol. The copolymerization reaction is performed using a hydrocarbon-based peroxide or azo compound polymerization initiator.
    Type: Grant
    Filed: March 6, 2014
    Date of Patent: October 31, 2017
    Assignees: Unimatec Co., Ltd., Hirosaki University
    Inventors: Katsuyuki Sato, Hideo Sawada
  • Publication number: 20170283778
    Abstract: The present disclosure relates to: an artificial peritoneal tissue comprising a cellular tissue and a mesothelial cell layer that covers the surface of the cellular tissue, wherein the cellular tissue comprises a fibroblast, an extracellular matrix, and a vascular endothelial cell and/or a lymphatic endothelial cell each capable of forming a lumen; and a method for producing the artificial peritoneal tissue.
    Type: Application
    Filed: August 25, 2015
    Publication date: October 5, 2017
    Applicants: HIROSAKI UNIVERSITY, OSAKA UNIVERSITY
    Inventors: Hiroshi SHIMODA, Yoshiya ASANO, Mitsuru AKASHI, Michiya MATSUSAKI
  • Patent number: 9756791
    Abstract: A grafting technique effective for crops such as soybean and plants of the Poaceae family including rice, wheat, corn, etc. and a method of grafting crops of the present invention comprising the steps of replacing a radicle of a dry dormant seed with a radicle of another dormant seed capable of achieving grafting, and thereafter, germinating the seed.
    Type: Grant
    Filed: November 7, 2013
    Date of Patent: September 12, 2017
    Assignee: HIROSAKI UNIVERSITY
    Inventor: Takeo Harada