Abstract: The present disclosure provides, among other things, immune suppression regimens for in vivo gene therapy and uses thereof. In various embodiments of the present disclosure, in vivo gene therapy includes delivery of at least one exogenous coding nucleic acid sequence to a stem cell of the subject. Success of in vivo gene therapy can be inhibited or reduced by immunotoxicity. The present disclosure provides compositions and methods, including among other things immune suppression regimens, that reduce immunotoxicity of in vivo gene therapy, e.g., in vivo gene therapy including administration of a viral gene therapy vector to a subject.

Abstract: A vehicle door comprising a mount, a encapsulated fixed window module, a movable window, and a sliding joint, the windows comprising outer surfaces which are aligned with each other as well as with an outer surface of a first trim attached to the first ascending strand and fixed to the first ascending post, and an outer surface of a second trim attached to the second ascending strand and fixed to the ascending profile.

Abstract: A camera lens suspension assembly includes a support member including a support metal base layer, a moving member including a moving metal base layer, bearings and smart memory alloy wires. The support member includes a bearing plate portion, static wire attach structures, and mount regions. A printed circuit on the support metal base layer includes traces extending to each static wire attach structure. The moving member includes a moving plate portion, elongated flexure arms extending from a periphery of the moving plate portion and including mount regions on ends opposite the moving plate portion, and moving wire attach structures. The bearings are between and engage the bearing plate portion of the support member and the moving plate portion of the moving member. Each of the smart memory alloy wires is attached to and extends one of the static wire attach structures and one of the moving wire attach structures.

Abstract: An apparatus is described. The apparatus includes a substrate and one or more sensor components formed on the substrate. And, the apparatus includes one or more electrical circuits formed on the substrate electrically coupled with at least one of the one or more sensor components formed on the substrate.

Abstract: In some aspects, the disclosure provides antibody-based reagents that specifically bind a tropomyosin receptor kinase B (TrkB) isoform that is associated with cancers. In some embodiments, the isoform is TrkB.T1. In some embodiments, the antibody or antibody derivative specifically binds a polypeptide comprising, consisting essentially of, or consisting of the sequence FVLFHKIPLDG (SEQ ID NO:1), or a sequence with at least 80% sequence identity thereto. In other aspects, the disclosure provides methods of producing the antibody or antibody derivative, related hybridomas, and methods of detecting and treating cancers incorporating use of the disclosed antibody reagents.

Abstract: The present disclosure describes an immunotherapy delivery hydrogel system. The immunotherapy delivery hydrogel system can be degradable and can release therapeutic agents at a tunable rate, and in a controlled manner. The immunotherapy delivery hydrogel system includes a hydrogel matrix and cancer therapeutic agent(s) associated with the hydrogel matrix. The hydrogel system can further include tumor cell-attractant(s) conjugated to the hydrogel matrix. The tumor cell-attractant(s) and the cancer therapeutic agent(s) act synergistically to treat cancer.

Abstract: Genomic safe harbors (GSH) for genetic therapies in human stem cells and engineered nanoparticles to provide targeted genetic therapies are described. The GSH and/or associated nanoparticles can be used to safely and efficiently treat a variety of genetic, infectious, and malignant diseases.

Abstract: A fluidic connection device for a fluid circuit of a motor vehicle includes a female fluidic connector, a U-shaped lock, and a locking indicator. The female fluidic connector includes a housing configured to receive a male fluidic connector, by translation along an axis (A). The U-shaped lock is carried by the female connector and includes a pair of elastically deformable legs that arranged substantially in a first plane, perpendicular to the axis (A) and on either side of the axis (A) and being configured to cooperate by elastic snap-fitting with the male connector to ensure retention of the male connector in the female connector along the axis (A). The locking indicator is carried by the female connector and configured to be translated in a second plane, perpendicular to the axis (A), between a first position for releasing the locking indicator and a second position for locking the locking indicator.

Abstract: A system and methods for manufacturing devices such as flexures using process coupons are described are described. The method including performing a test on at least one feature of a coupon, the coupon is included on an assembly sheet used in manufacturing flexures. The at least one feature is produced by a manufacturing processing step that is used to produce a portion of a flexure. And, the physical characteristics of the feature include at least one physical characteristic that is different than physical characteristics of the portion. The method also including determining the manufacturing processing step will produce an abnormal portion of a flexure based on the performed test. Further, the method includes adjusting the manufacturing processing step and manufacturing a portion of a flexure using the adjusted manufacturing processing step.

Abstract: Systems and methods to genetically modify B cells to express selected antibodies are described. The systems and methods can be used to: obviate the need for classical vaccinations; provide protection against infectious agents for which no vaccinations are currently available; provide protection against infectious agents when patients are otherwise immune-suppressed; and/or provide a benefit provided by a therapeutic antibody, such as in the treatment of autoimmune disorders.

Abstract: Systems and methods to increase the efficacy of vaccines that require or are rendered more effective with T cell mediated immunity are described. The systems and methods utilize polynucleotides that genetically modify T cells to express a T cell receptor specific for an administered vaccine antigen.

Abstract: Described herein are peptides and variants and mutants thereof capable of interacting with TEAD, disrupting the HIPPO pathway, or modulating the activity or function of TEAD interactions in a cell. Pharmaceutical compositions and uses of peptides, as well as methods of designing and manufacturing such peptides, to treat cancer, tumor, or any other disease/condition associated with a dysregulated HIPPO pathway or uncontrolled cell growth are also described herein.

Abstract: Circular handed alpha-helical repeat proteins are described. The repeat proteins have a number of uses as scaffolds for geometrically precise, arrayed presentation of cell-signaling or immune-related protein and peptide epitopes, as well as numerous other therapeutic, diagnostic, and nanotechnological uses.

Abstract: The present disclosure provides binding proteins and TCRs with high affinity and specificity against Merkel cell polyomavirus T antigen epitopes or peptides, T cells expressing such high affinity Merkel cell polyomavirus T antigen specific TCRs, nucleic acids encoding the same, and compositions for use in treating Merkel cell carcinoma.

Abstract: A camera assembly comprises a lens assembly supported on a support structure, wherein the lens assembly includes an autofocus actuator arrangement and the camera assembly includes an optical image stabilization assembly arranged to move the lens assembly in a plane perpendicular to the optical axis. A flexible printed circuit tape connected between the support structure and the lens assembly and providing an electrical connection to the auto-focus actuator arrangement is bent around a corner, thereby allowing the flexible printed circuit tape to accommodate the motion of the lens assembly perpendicular to the optical axis. A crimp plate connected to the lens assembly which crimps shape memory alloy wires has features extending out of the plane of the crimp plate for reducing flexibility. At least part of the optical image stabilization assembly overlaps the lens assembly in the direction along the optical axis, thereby reducing the height of the camera assembly.

Abstract: Devices including a substrate and a plurality of coil portions disposed on the substrate. The plurality of coil portions electrically coupled to form a coil structure.

Abstract: Systems and methods to selectively protect therapeutic cells by reducing CD33 expression in the therapeutic cells using base editors and targeting non-therapeutic cells with an anti-CD33 therapy are described. The selective protection results in the enrichment of the therapeutic cells while simultaneously targeting any diseased, malignant and/or non-therapeutic CD33 expressing cells within a subject.

Abstract: Anti-vibration mount comprising a first frame, a second frame, an elastomeric main body connecting the first frame and the second frame, the elastomeric main body allowing relative displacements of the second frame relative to the first frame in a first direction of vibration and in a second direction of vibration perpendicular to the first direction of vibration, the elastomeric main body also being suitable for mounting a weight in the first direction of vibration. An inertia body is connected to the first frame by an elastic suspension interposed between the inertia body and the first frame along the second direction of vibration. An elastomeric lateral abutment body is interposed between the first frame and the inertia body to limit the relative displacements of the second frame with respect to the first frame in the second direction of vibration. The inertia body has a natural frequency lower than 800 Hz.

Abstract: A suspension assembly is described. A suspension assembly including a support member configured to receive at least a first circuit member. The first circuit member including at least a trace. The first circuit member disposed on the support member.

Abstract: A window module (60) for a vehicle, in particular automobile, comprising: a fixed window (22) comprising an internal face (22c) and an external face (22d), a movable window (14) comprising an internal face (14c) and an external face (14d), edges (14b, 22a) of the windows facing one another and defining a clearance (J) between them, and the external faces (14d, 22d) of the windows being substantially aligned, an elongated guide (26) extending along said edges and attached to the internal face (14c) of the movable window (14), an elongated upright (62) extending along said edges and attached to the internal face (22c) of the fixed window (22), and a trim element (70) fitted and attached to the upright, this trim element comprising at least one member (72) for retaining the guide in a housing (L) of the upright.