Abstract: A backside connection access structure and method for manufacturing are described. The method including forming a gold layer over at least a portion of a substrate. The method also including forming a metal layer over the gold layer. And, the method includes forming an opening in the substrate to expose at least a portion of the gold layer.

Abstract: A connector for providing a leak resistant flow path between fluid conduits includes a female connector, a male connector, and a tubular fluid conduit that extends through a conduit passage in the male connector and into a conduit receiving passage in the female connector. The connector can include optional anti-cantilever features that provide a more effective axial seal when using a seal washer recessed into a planar surface of the male connector to supplement a radial seal provided by an O-ring sealing member between the tubular fluid conduit and the conduit receiving passage.

Abstract: The instant disclosure provides biomarkers and methods for identifying subjects at risk of developing cytokine release syndrome (CRS), neurotoxicity, or both after adoptive immunotherapy to guide preemptive intervention, modified therapy, or the like. For example, adverse event biomarkers may be measured in a subject before pre-conditioning chemotherapy, before immunotherapy (e.g., adoptive immunotherapy infusion comprising a chimeric antigen receptor (CAR) modified T cell), or shortly after pre-conditioning chemotherapy and/or immunotherapy. Exemplary biomarkers include temperature, cytokine levels and endothelial activation biomarkers, such as angiopoietin 2, von Willebrand factor (vWF), ratio of angiopoietin 2 to angiopoietin 1, and ratio of ADAMTS13 to vWF. Also provided are methods of treating subjects identified as at risk of developing cytokine release syndrome (CRS), neurotoxicity, or both to minimize such potential adverse events.

Abstract: A method for detecting the binding of a chromatin-associated factor of interest to a sequence of chromatin DNA in a cell, including: contacting a permeabilized cell or nucleus with a specific binding agent that specifically recognizes the chromatin-associated factor of interest, wherein the specific binding agent is linked to a nuclease that is inactive or an activatable transposome; activating the nuclease or transposase, thereby excising the sequence of chromatin DNA bound to the chromatin-associated factor of interest; isolating the excised DNA; and determining the sequence of the excised DNA, thereby detecting binding of a chromatin-associated factor of interest to a sequence of chromatin DNA in the cell.

Abstract: The present disclosure provides compositions and methods for targeting a Ras antigen to, for example, treat or prevent cancer. Disclosed embodiments include binding proteins, such as a T cell receptor or a chimeric antigen receptor, that bind to a Ras antigen:HLA complex. Polynucleotides encoding such binding protein can introduced into a host cell, such as a T cell, and the cell can be used in immunotherapy for treating various cancers. Also provided are immunogenic polypeptides that can be useful to, for example, induce an immune response against a mutated Ras or to identify a binding protein that binds to a Ras antigen.

Abstract: A device includes a dielectric layer having a first surface and a second surface. The device also includes a first set of high-aspect ratio electroplated structures disposed on the first surface of the dielectric layer and a second set of high-aspect ratio electroplated structures disposed on the second surface of the dielectric layer opposite the first set of high-aspect ratio electroplated structures.

Abstract: A method for detecting the binding of a chromatin-associated factor of interest to a sequence of chromatin DNA in a cell, including: contacting a permeabilized cell or nucleus with a specific binding agent that specifically recognizes the chromatin-associated factor of interest, wherein the specific binding agent is linked to a nuclease that is inactive or an activatable transposome; activating the nuclease or transposase, thereby excising the sequence of chromatin DNA bound to the chromatin-associated factor of interest; isolating the excised DNA; and determining the sequence of the excised DNA, thereby detecting binding of a chromatin-associated factor of interest to a sequence of chromatin DNA in the cell.

Abstract: Hematopoeitic stem/progenitor cells (HSPC) and/or non-T effector cells are modified to express an extracellular component including a tag cassette. The tag cassette can be used to activate, promote proliferation of, detect, enrich, isolate, track, deplete and/or eliminate modified cells. The cells can also be modified to express a binding domain.

Abstract: A method provides a sealed connection of a connector to a heat exchanger of the coaxial tubular type is particularly suitable for a motor vehicle air-conditioning circuit. The method includes the steps of mounting a free end of an external tube of the exchanger in or on the connector. The external tube is directly secured with the connector, and an internal tube is inserted in the external tube until a free end of the internal tube is mounted in or on the connector. This mounting ensuring a sealing between the internal tube and the connector. The method further includes directly securing the internal and external tubes against one another to avoid relative displacements.

Abstract: The present disclosure provides methods for immortalizing precursor cells that are non-terminally differentiated cells such as stem cells, the methods comprising culturing the precursor cells in the presence of a Notch 1 agonist, Notch 2 agonist or Notch 1 agonist and Notch 2 agonist (and, in particular embodiments, one or more growth factors) that support the proliferation but not differentiation of the non-terminally differentiated cells. The present disclosure further provides methods to induce the differentiation of immortalized cells, comprising growing the cells in the presence of a Notch 1 agonist, Notch 2 agonist or Notch 1 agonist and Notch 2 agonist and at least one growth factor which supports the differentiation of the cell into a more specialized cell type. The immortalized and/or differentiated cells of the disclosure can be used to repopulate cell populations that have been diminished, for example as a result of infection or exposure to certain drugs.

Abstract: Guide (114) fora sliding glazing, in particular for an automobile, said guide (114) being intended to be fastened onto a glazing (12) and to cooperate with a glass run seal (10), said guide (114) comprising a body made of a single piece and having an elongated shape along an axis (A), said body comprising: a fastening portion (114a) extending along said axis (A) and having a face (114a1) configured to be fastened onto the glazing (12), and a guide portion (114b) extending along said axis (A) and being cross sectional L-shaped, said guide portion (114b) comprising a wall (114b1) substantially parallel to said face (114a1) and a free rib (114b2) substantially perpendicular to said face (114a1), said free rib (114b2) including at least one non-planar lateral face (128, 130).

Abstract: Siderocalin-metal chelator combinations that bind metallic radioisotopes used in nuclear medicine with high affinity are described. The high affinity siderocalin-metal chelator combinations include a number of chelator backbone arrangements with functional groups that coordinate with metals. The siderocalin-metal chelator combinations can be used to deliver radionuclides for imaging and therapeutic purposes.

Abstract: Devices and methods for bio-specimen refrigeration are provided. In an embodiment, the bio-specimen refrigeration devices of the present disclosure include a housing having a lid and a base portion, wherein the lid is selectively moveable between an open position and a closed position; a coolant cartridge chamber disposed in the housing and configured to fluidically couple with a coolant cartridge disposed in the coolant cartridge chamber; and a cooling chamber disposed in the housing and configured to receive a fluid coolant from the coolant cartridge, wherein in the closed position, the lid seals the cooling chamber.

Abstract: The guide for sliding glazing intended to be added and fastened onto a glazing and to cooperate with a sliding joint. The guide comprising a body of elongated shape and a longitudinal base extending along the axis of elongation of the body. A first face of the guide applied and fastened onto a glazing surface and a second face opposite the first face, the second face cooperating by sliding with said joint. The longitudinal base including a thinned portion at at least one free longitudinal end. A thickness of the thinned portion, in a direction substantially perpendicular to said first face, decreasing at least in the longitudinal direction in such a way that said second face is flush with said glazing surface on said at least one free end.

Abstract: Methods of making a weather strip and bright strip assembly for a vehicle door include extruding a weather strip that has a longitudinal shape along an X axis, a sealing body made of at least a first material, and a carrier body made of at least a second material that is harder than the first material. The methods further include cutting at least in part a longitudinal end of the carrier body along the X axis, and removing the longitudinal end; molding a curved or angled end onto the weather strip, in replacement of the longitudinal end, wherein the curved or angled end extends between the X axis and a Y axis; and mounting a bright strip onto the carrier body, the bright strip including a first curved or angled portion covering at least in part the curved or angled end and a second longitudinal portion covering at least in part the carrier body.

Abstract: The current disclosure provides protein residue mapping using a combination of deep mutational scanning and phage display high throughput sequencing. The disclosed methods allow mapping of antibody epitopes and determination of changes in residues of a protein that abolish binding of the protein to a candidate binding molecule.

Abstract: SMA actuators and related methods are described. One embodiment of an actuator includes a base; a plurality of buckle arms; and at least a first shape memory alloy wire coupled with a pair of buckle arms of the plurality of buckle arms. Another embodiment of an actuator includes a base and at least one bimorph actuator including a shape memory alloy material. The bimorph actuator attached to the base.

Abstract: Methods and compositions for treatment of malignancies are provided. The methods utilize implantation of engineered, programmable hydrogel depots capable of long-term molecule release into close proximity of the tumor. By providing gradients of immune cell chemokines and releasing immune checkpoint inhibitors, the hydrogel implants are effective at elimination of tumor cells via immune cell-mediated cell death.

Abstract: The invention relates to a material including a support consisting of a porous composite material including at least one polymer phase forming a binder based on at least one polymer selected from thermoplastic polymers, elastomers, and elastomer thermoplastics, and at least one filler selected from thermally conductive fillers, the pores of the support consisting of the porous composite material being partially or entirely filled with at least one phase-change material. The invention also relates to a method for producing said material.

Abstract: The invention relates to a heat exchanger comprising a first free space (7) for a first fluid (3), a thermally conductive wall (11) which, at least locally, delimits said first free space (7), in such a way that an exchange of heat can occur between the first fluid and the thermally conductive wall (11) which is hollow and encloses a material (13) for storing thermal energy by accumulation of latent heat, by heat exchange with at least the first fluid. The first free space (7) is divided into at least two separated channels (7a, 7b) in which two streams of the first fluid (3) can circulate at the same time but separately, the thermally conductive wall (11) which encloses the thermal energy storage material (13) being interposed between the two channels (7a, 7b).