Abstract: An embodiment is disclosed for performing the image processing for analyzing the results of a fluorescence in situ hybridization (FISH) microscopic automated sample analysis to determine specific chromosomal characteristics.
Type:
Application
Filed:
July 12, 2011
Publication date:
December 29, 2011
Applicant:
IKonisys, Inc.
Inventors:
Yanning Zhu, Triantafyllos Tafas, Youngmin Kim, Xiuzhong Wang
Abstract: An improved filter wheel is disclosed, through embodiments, that comprises a plurality of reflector support structures formed as a unitized structure with a circular base member. In some embodiments, the filter wheel further comprises a plurality of light sources mounted on the filter wheel.
Abstract: An embodiment is disclosed for performing the image processing for analyzing the results of a fluorescence in situ hybridization (FISH) microscopic automated sample analysis to determine specific chromosomal characteristics.
Type:
Grant
Filed:
August 2, 2007
Date of Patent:
August 16, 2011
Assignee:
IKONISYS, Inc.
Inventors:
Yanning Zhu, Triantafyllos Tafas, Youngmin Kim, Xiuzhong Wang
Abstract: A method for recognizing an object in an image is disclosed wherein a fractal map of the image is generated by estimating the fractal dimension of each pixel in the image. The fractal map may be segmented by thresholding and locations of candidate objects are determined. The pixel value of the image pixel corresponding to the same location where the candidate object is found in the fractal map may be compared to a threshold value. If the pixel value is greater than the threshold value, the candidate object is recognized as a valid object.
Type:
Grant
Filed:
February 7, 2011
Date of Patent:
August 2, 2011
Assignee:
Ikonisys Inc.
Inventors:
Triantafyllos P. Tafas, Michael Kilpatrick, Petros Tsipouras
Abstract: A method for recognizing an object in an image is disclosed wherein a fractal map of the image is generated by estimating the fractal dimension of each pixel in the image. The fractal map may be segmented by thresholding and locations of candidate objects are determined. The pixel value of the image pixel corresponding to the same location where the candidate object is found in the fractal map may be compared to a threshold value. If the pixel value is greater than the threshold value, the candidate object is recognized as a valid object.
Type:
Application
Filed:
February 7, 2011
Publication date:
June 2, 2011
Applicant:
Ikonisys, Inc.
Inventors:
Triantafyllos Tafas, Michael Kilpatrick, Petros Tsipouras
Abstract: An filter, apparatus and method for preparing microscope slides is presented. After filtration of the specimen is performed, the filter containing the filtrand is configured to be microscopically examined. The filter may either be affixed to a microscope slide or, in an embodiment, the filter may be incorporated into a microscope slide.
Type:
Application
Filed:
July 9, 2010
Publication date:
May 26, 2011
Applicant:
IKONISYS, INC.
Inventors:
Michael Thomas, Charles Climer, Richard Schlueter, Triantafyllos P. Tafas, Michael Kilpatrick, Antti Seppo
Abstract: An automated cassette and slide handling system is disclosed which organizes microscope slides in cassettes, automatically and sequentially removes individual slides from their respective cassettes, positioned each slide under the microscope as provided by the protocol, and after examination returns the slide to its proper cassette.
Type:
Grant
Filed:
August 3, 2007
Date of Patent:
May 24, 2011
Assignee:
Ikonisys, Inc.
Inventors:
Tibor Virag, Yash Agarwal, Wei Guo, Richard Eberle, Youngmin Kim, Michael Kilpatrick, Petros Tsipouras, Triantafyllos Tafas
Abstract: A method for automated microscopic analysis wherein the test protocol is obtained from interrogatable data affixed to each microscope slide. The method further comprises the algorithms that implement the test protocol.
Type:
Application
Filed:
August 12, 2010
Publication date:
April 7, 2011
Applicant:
Ikonisys, Inc.
Inventors:
Young Min KIM, Yanning ZHU, Yash AGARWAL, Xiuzhong WANG, Aaron ARMSTRONG, Robert BORGERDING, Andrew MACGINITIE, Antti SEPPO, llia ICHETOVKIN, Michael KILPATRICK, Petros TSIPOURAS, Triantafyllos TAFAS
Abstract: A displaceable light-tight enclosure system housing an automated robotic microscope having electronically controllable components and an image capture device. The enclosure system includes a device(s) for displacement, an externally viewable monitor, and a plurality of electrical power receptacles. The light-tight enclosure system also incorporates an anti-fall support leg that may be extended from the enclosure.
Type:
Grant
Filed:
December 8, 2008
Date of Patent:
March 8, 2011
Assignee:
Ikonisys, Inc.
Inventors:
Triantafyllos Tafas, Yash Agarwal, Youngmin Kim, Wei Guo, Richard Eberle, Joseph Turgeon, Michael Kilpatrick, Petros Tsipouras
Abstract: In various embodiments methods for automated screening for gene amplification in biological tissue samples using an automated fluorescence microscope to analyze fluorescence in situ hybridized samples are provided. Various additional embodiments provide methods of high throughput screening for gene amplification.
Type:
Grant
Filed:
March 19, 2008
Date of Patent:
March 8, 2011
Assignee:
Ikonisys, Inc.
Inventors:
Triantafyllos P. Tafas, Michael Kilpatrick, Xiuzhong Wang, Youngmin Kim, Michael Thomas, Petros Tsipouras
Abstract: An imager system is disclosed comprising a image intensifier and a CMOS image sensor. The system provides fast capture speed and high sensitivity.
Abstract: A method for recognizing an object in an image is disclosed wherein a fractal map of the image is generated by estimating the fractal dimension of each pixel in the image. The fractal map may be segmented by thresholding and locations of candidate objects are determined. The pixel value of the image pixel corresponding to the same location where the candidate object is found in the fractal map may be compared to a threshold value. If the pixel value is greater than the threshold value, the candidate object is recognized as a valid object.
Type:
Grant
Filed:
March 21, 2008
Date of Patent:
February 8, 2011
Assignee:
Ikonisys Inc.
Inventors:
Triantafyllos P. Tafas, Michael Kilpatrick, Petros Tsipouras
Abstract: A computer controlled method for detecting and diagnosing a rare cell type in a tissue sample is provided, said method comprising treating the tissue sample such that it generates a first signal indicative of the presence at a location of a rare cell, detecting the first signal, treating the location at which the first signal is detected to generate a second signal indicative of a diagnostically useful cellular characteristic and detecting the second signal. The first signal can be morphological or a color present in a sought cell either before or after staining. The second signal can be generated by in situ PCR or PCR in situ hybridization. In one preferred embodiment, the rare cell type is a fetal cell in a maternal blood tissue sample, said sample consisting of a smear of unenriched maternal blood. In another embodiment, the method is used to diagnose or genotype cancer cells in a blood or tissue biopsy sample.
Abstract: A method for automated microscopic analysis wherein the test protocol is obtained from interrogatable data affixed to each microscope slide. The method further comprises the algorithms that implement the test protocol.
Type:
Grant
Filed:
August 3, 2007
Date of Patent:
November 9, 2010
Assignee:
Ikonisys, Inc.
Inventors:
Young Min Kim, Yanning Zhu, Yash Agarwal, Xiuzhong Wang, Aaron Armstrong, Robert Borgerding, Andrew MacGinitie, Antti Seppo, Ilia Ichetovkin, Michael Kilpatrick, Petros Tsipouras, Triantafyllos Tafas
Abstract: The present invention relates generally to a fractionator for collecting at least one of a plurality of segregated components from a segregated sample disposed in a sample tube. The fractionator has a head with a head surface at its forward end. The head may be configured to form a slideable seal with the inside surface of a sample tube. A collection port is disposed forward of the head surface, and a fluid passageway is in fluid communication with the collection port and is configured and arranged to allow fluid transport from the sample tube to a sample receptacle. The fractionator may have a valve in fluid communication with the collection port and may have a valve controller configured and arranged to operate the valve based, at least in part, on the location of the collection port with respect to a sample disposed in the sample tube.
Abstract: A method and apparatus for automating microscopic analysis of a plurality of data-encoded microscope slides. In embodiments, the data written to or read from the slides may comprise images, analysis protocols, analytic results and other pertinent data. Embodiments also encompass a magazine that contains a plurality of data encoded slides.
Abstract: A method and apparatus for automating microscopic analysis of a plurality of data-encoded microscope slides. In embodiments, the data written to or read from the slides may comprise images, analysis protocols, analytic results and other pertinent data. Embodiments also encompass a magazine that contains a plurality of data encoded slides.
Abstract: The invention herein includes a method for combining immuno staining and FISH using covalently bound small molecule tags. Among embodiments included are methods of tagging immuno and FISH probes for treatment of biologic material. Embodiments comprise directing the fluorescently labeled immuno probe(s) to non-chromosomic portions of a biologic sample and directing labeled FISH probe(s) to chromosomic portions of the biologic sample for further identification of subcellular components. Embodiments also comprise the automatic connection of information regarding the sample to image analysis, to subcellular component identification and enumeration in order to affect biomedical decisions.
Type:
Grant
Filed:
March 13, 2007
Date of Patent:
February 9, 2010
Assignee:
Ikonisys, Inc.
Inventors:
Michael Kilpatrick, Antti Seppo, Triantafyllos Tafas