Abstract: Disclosed is a processing method of a self-reinforced starch-based multifunctional material, and belongs to the technical field of starch deep processing. The processing method takes bulk starch as a base material, including the following steps: firstly reacting starch nanoparticles with an organic acid anhydride reagent and adding a bacteriostatic agent to prepare composite nanoparticles, then mixing the composite nanoparticles with the bulk starch, an etherifying agent, a crosslinking agent, a plasticizer and the like, and finally preparing a starch-based multifunctional material by dry extrusion modification combined with a starch-based nanoparticle assembly and reinforcement technology. The method of the disclosure is simple and convenient in step, mild and controllable in reaction, and continuous and green in production.

Abstract: The present disclosure discloses preparation and application of a novel multifunctional nanocomposite material with new photosensitizer, and belongs to the technical field of photodynamic therapy and the field of biomedicine. The photosensitizer multifunctional nanocomposite material provided by the present disclosure is prepared by self-assembly of cercosporin and an acid-sensitive copolymer multifunctional material with liver tumor cell targeting ability and traceability, wherein the acid-sensitive copolymer multifunctional material can be a copolymer of poly(N,N-dimethylaminoethyl methacrylate) and poly-3-azido-2-hydroxypropyl methacrylate covalently linked by galactose-modified rhodamine B.

Abstract: The present disclosure discloses a rosin-based small molecular weight hydrogelator and an application thereof, and belongs to the fields of supramolecular chemistry, surfactant science and chemical utilization of rosin. The rosin-based small molecular hydrogel of the present disclosure can gel water at a very low concentration, and the critical gelling concentration is only 0.176 wt %. On average, each gelling agent molecule can hold 13,889 water molecules, which exhibits extremely high gel efficiency and the formed small molecular hydrogel also exhibits extremely high stability. This small molecule hydrogel is derived from the natural product rosin and has a mild nature. It can be used in the fields of drug sustained-release, tissue engineering, daily chemicals, medicine and so on.

Abstract: The disclosure discloses an apparatus and a method for microbial cell counting, and belongs to the field of cell counting. In the present application, by converting a traditional automated intermittent counting process into a continuous counting process, the cell sap fixed in a blood cell plate in a traditional counter becomes the cell sap flowing in a microchannel, so as to prolong the cell detection time and distance. The size of the microchannel is slightly greater than the diameter of microbial cells, so as to ensure that the cells flow through the cross section of the microchannel one by one. At the same time, since the diameter of the counterbores communicated by the microchannel is slightly greater than the width of the microchannel, the flow rate of the cell sap slows down when the cell sap flows to the counterbores.

Abstract: The present disclosure discloses a processing method for intelligent hydrogel from nano-scale starch particles, and belongs to the technical field of nutritional health food. The present disclosure uses dendritic water-soluble starch particles as a skeleton and utilizes a transglycosidation and chain extension-glycan entanglement reaction of glycosyltransferase to obtain an intelligent hydrogel having a spatial reticular structure. The product provided by the present disclosure is an intelligent starch-based hydrogel which has good rehydration capability, biocompatibility, strong gel strength, enzymatic response irreversibility, pH response reversibility, can carry multiple nutritional factors. The hydrogel provided by the present disclosure can protect and control the release of food functional factors, and can be applied to food, biological drug loading, functional materials and the like.

Abstract: The disclosure discloses a method for near-infrared spectral wavelength selection based on an improved team progress algorithm (iTPA), belonging to the field of near-infrared spectral detection. The method includes: equally dividing near-infrared spectral wavebands to be selected into elite groups, ordinary groups and garbage collection groups according to evaluation values from high to low; generating a new waveband in the elite group or the ordinary group, where a wavelength point of the new waveband is selected from a random waveband in the selected group, and the wavelength point of the new waveband inherits the selected wavelength point; and enabling the inherited new waveband to select a learning behavior or an exploration behavior according to a set probability to update the wavelength point of the new waveband to generate a candidate waveband; and selecting a waveband with a highest evaluation value in the elite group as the waveband to be selected.

Abstract: The present application belongs to the technical field of research and development of fermented beverages, and a method for preparing Cordyceps militaris ferment by two-stage fermentation and complex enzymatic hydrolysis is provided. The present application involves preparation of a Cordyceps militaris powder and a Cordyceps militaris slurry, preparation of a Cordyceps militaris fermentation substrate, lactic acid bacteria fermentation in combination with complex enzymatic hydrolysis, and yeast fermentation, followed by centrifugation, filtration, formulation, sterilization, and filling to obtain a product. By employing lactic acid bacteria fermentation in combination with complex enzymatic hydrolysis, and secondary fermentation by yeast, the present application can quickly finish a whole fermentation process in 2 to 3 days.

Abstract: A preparation method for a water-retaining nanoagent and applications thereof in a quick-frozen crustacean shrimp product, related to the technical field of food processing. The formula of the present invention comprises inulin, xylitol, carboxymethyl chitosan, pullulan, sodium tripolyphosphate, sodium chloride, and sodium alginate. The powder raw materials are mixed evenly with water and then go through a homogenizer, a colloid mill, and an ultrasound treatment to produce the water-retaining nanoagent. The method of the present invention also relates to applications of the freeze-proof water-retaining agent in freezing a crustacean aquatic product.

Abstract: A method for evaluating crease recovery of fabrics based on power function equation. The steps are: (1) place the sample in the sample placement area; (2) pressure the overlapping part of the sample; (3) let the free part of the sample automatically restore and record the video image of the sample crease recovery by camera; (4) process the video image of the fabric crease recovery and calculating the recovery angle of each frame of video image; (5) repeat steps 1 to 4 to measure N samples of the same fabric; (6) obtain the dynamic process of fabric crease recovery angle change. This can reveal which type of fabric has better recovery property, when the existing methods have the similar results of recovery angle.

Abstract: The disclosure discloses a nitrile hydratase mutant, a genetically engineered bacterium containing the mutant and applications thereof, and belongs to the technical field of enzyme engineering. In the disclosure, glycine at position 47 of a nitrile hydratase mutant ?L6T/A19V/F126Y-?M46K/E108R/S212Y (disclosed in the patent of disclosure CN102216455A) is mutated to asparagine. The obtained new mutant enzyme has better temperature tolerance and tolerance to a product, and is conducive to future industrial production. The recombinant strain containing the nitrile hydratase mutant is fermented at high density, and 3-cyanopyridine is used as a substrate to carry out a whole-cell catalytic reaction to prepare nicotinamide. Compared with a chemical production method, the method has a safe and clean production process and no environmental pollution. Compared with an enzymatic method, the substrate price is cheap and the catalytic efficiency is high.

Abstract: Disclosed is a method for promoting extracellular expression of proteins in B. subtilis using cutinase, which belongs to the technical fields of genetic engineering, enzyme engineering and microbial engineering. It teaches co-expressing a cutinase mutant and a target protein in B. subtilis to promote extracellular expression of the target protein which is naturally located inside cells. The target protein includes xylose isomerase, 4,6-?-glucosyltransferase, 4-?-glucosyltransferase, trehalose synthase, branching enzyme and the like. The invention can achieve extracellular expression of intracellularly localized target protein, improve the production efficiency, reduce the production cost and simplify the subsequent extraction process.

Abstract: The disclosure discloses a method for preparing a lipoamino acid compound based on deep eutectic solvents, and belongs to the field of organic synthesis. The preparation method of the disclosure uses a fatty acid and an amino acid as substrates, uses a deep eutectic solvent as a medium, and directly and efficiently prepares a single-chain lipoamino acid surfactant under the catalysis of lipase CALB. Alternatively, Amano lipase M is used as a catalyst to efficiently catalyze the reaction of an amino acid containing a plurality of amino groups with a fatty acid to prepare a Gemini amino acid. The method of the disclosure does not need to undergo protection and deprotection of the amino acid, and does not involve commonly used fatty acid chlorides. Compared with the traditional chemical synthesis methods, the disclosure has the advantages of greenness, safety, high selectivity, high yield, etc., and has very important potential application value.

Abstract: The disclosure provides an active peptide with an anti-lipid oxidation function and a preparation method and application thereof and belongs to the technical field of plant-derived biologically active peptides. In the disclosure, oil processing by-products, namely oil crops after oil extraction, are used as the raw materials, and the raw materials are subjected to the steps of protein extraction, infrared pretreatment, proteolysis, freeze-drying, lipophilic part extraction, vacuum concentration and drying to prepare an anti-lipid oxidation peptide having the functional characteristics of scavenging DPPH free radicals, chelating metal ions, inhibiting lipid peroxidation, prolonging vegetable oil oxidation induction time, improving emulsion stability and the like.

Abstract: The present invention discloses a cutting auxiliary device integrated with clamping and blanking functions includes a positioning and loading unit, a clamping unit and a supporting unit. The supporting unit and the clamping unit can slide left and right on the platform of the positioning and loading unit through the slide block and slide groove structure. The supporting unit and the clamping unit are connected through an adjustable push rod with a scale bar; The board is placed on the positioning platform, and the adjustable push rod is pushed to move the supporting unit and the clamping unit to fix the board again for cutting. After completion, the handwheel is turned to make the clamping platform rotate such that the material is transferred into the material receiving container.

Abstract: The present invention discloses a glucan-based shell-core structure carrier material and preparation and application thereof, and belongs to the technical field of modern food processing. Spherical hyperbranched water-soluble amylum grains are used as the raw material, and an enzymatic grafting and chain extending process is adopted for treatment to modify the surfaces of water-soluble glucan molecules into a firm shell structure with densely cumulated crystal structures, and form the glucan-based carrying material with the shell-core structure of which an inner core cavity has an amorphous state and an outer shell layer has a crystalline state.

Abstract: The present disclosure discloses a preparation method of low-glycemic index slowly digestible starch-based reconstituted instant rice, and belongs to the technical field of food processing. The preparation method is based on high-temperature fluidization technology to carry out non-crystallization treatment on a solid phase broken rice raw material in a short period of time, and then the material is subjected to a one-step reactive extrusion technology combining enzymatic hydrolysis and esterification treatment to prepare the reconstituted instant rice with high content of slowly digestible starch and low glycemic index. The reconstituted instant rice prepared by the one-step molding extrusion technology is suitable for type II diabetes patients to eat, and at the same time increases the added value of agricultural and sideline products, reduces production cost, and improves production efficiency.

Abstract: A teaching and demonstration tool for simulating fabric construction, which is used for demonstrating the fabric construction and assisting in teaching and designing fabric constructions. The teaching and demonstration tool includes a frame body for fixing the warp strips (6) and weft strips (7), and a plurality of pairs of positioning members (3). The frame body includes longitudinal bars (1) and latitudinal bars (2). A plurality of warp strips (6) and weft strips (7) are fixed on the longitudinal bars (1) and the latitudinal bars (2) respectively by the plurality of positioning members (3). Both the longitudinal bars and latitudinal bars (1, 2) are provided with scale lines.

Abstract: Disclosed is a preparation method for polylactic acid grafted chitosan nanowhiskers, and belongs to the technical field of materials. The preparation method of the disclosure is that after lactide, a catalyst and chitosan are uniformly mixed, polymerization grafting is performed to prepare PLA-g-CS, and then the PLA-g-CS is dispersed into an alkali liquor to obtain nanowhiskers by a repeated freezing/unfreezing method, with no solvent used in a polymerization grafting process. The method has advantages that the nanowhiskers can be prepared from the PLA-g-CS without a good solvent, and the whole reaction is efficient, clean, and environmentally friendly.

Abstract: The present invention provides a recombinant Bacillus subtilis with improved 2?-fucosyllactose production, and a construction method thereof. In the present invention, a strain capable of efficiently synthesizing 2?-fucosyllactose is obtained by the fusion expression of the fucosyltransferase gene and the L-fucokinase/guanosine 5?-diphosphate-L-fucose pyrophosphorylase gene in Bacillus subtilis BSGL-FF, the fermentation supernatant of which comprises a cumulative amount of 2?-fucosyllactose as high as 1.62 g/L, which is 55% higher than the amount achieved with the control strain. The construction method of the recombinant Bacillus subtilis of the present invention is simple, and convenient to use, and thus has good application prospects.

Abstract: The present disclosure relates to estimation methods of actuator faults based on Bayesian learning. An actuator fault is modeled based on a random walking model, and a joint posterior probability distribution of a system state variable and the actuator fault is represented using two mutually independent hypothesis distributions based on a variational Bayesian theory; a system state variable and an actuator fault of a system at a moment are predicted at a moment; and a predicted system state variable and a predicted actuator fault are iteratively updated at the moment according to the Bayesian theory to output an estimated value of the system state variable at the moment, a variance of the estimated value and an estimated value of the actuator fault at the moment and a variance of the estimated value.