Abstract: The disclosure discloses an integrated riparian system for river/lake silt on-site treatment and application thereof, belonging to the technical field of silt treatment. The integrated riparian system includes three subsystems, namely a silt ecological treatment subsystem, a siphon drainage subsystem and a silt leachate advanced treatment subsystem. The silt ecological treatment subsystem is provided with plants, fillers, an aeration pipe and a water collection pipe from top to bottom, and a vent pipe is connected to the water collection pipe. A siphon in the siphon drainage subsystem is retractable and hump-shaped. The silt leachate advanced treatment subsystem includes a first-stage ecological treatment unit upflow wetland and a second-stage ecological treatment unit surface flow wetland.

Abstract: The disclosure discloses a high-flux and ultra-sensitive detection dot array enhancement chip, and belongs to the field of food safety detection. In the disclosure, single-layer Au nano-particles are chemically bonded onto a hydrophilic substrate, an Au nano-material is naturally deposited in holes of the chip under an electrostatic adsorption action, and a regular dot array is formed. Au particles distributed in the holes are separated with a particle surfactant (CTAB) to form plasma gaps so as to enhance the self-assemble of Au nano-particles distributed on hot-spots for a long range effect, thereby improving the sensing signal in detection efficiency and sensitivity of the chip.

Abstract: The disclosure discloses a method for chemically synthesizing a Helicobacter pylori core lipopolysaccharide oligosaccharide antigen carbohydrate chain, and belongs to the technical field of carbohydrate chemistry. The disclosure uses D-glucose, D-galactose and D-mannose as starting materials, which undergo a series of protection and deprotection reactions to prepare eight monosaccharide blocks. The eight monosaccharide block compounds as shown in formulas 2 to 9 undergo glycosylation reactions under catalysis of corresponding activating reagents, to prepare H. pylori lipopolysaccharide core oligosaccharide antigen fragments as shown in formula 1. The disclosure further combines the synthesized oligosaccharide fragments with a chip to make a carbohydrate chip, screens the optimal antigen fragments using patient serum, or combines the synthesized oligosaccharide fragments with carrier proteins to make glycoconjugates.

Abstract: A fermented beverage and a preparation method are disclosed. The method uses ripened fruits and/or vegetables as raw materials. The method includes performing a natural ripening on a fruit and/or a vegetable at 15° C. to 30° C. for 5 days to 10 days to obtain a ripened fruit and/or vegetable; and inoculating lactic acid bacteria into the ripened fruit and/or vegetable, and performing a fermentation for 10 days to 30 days at 15° C. to 30° C. to obtain the fermented beverage. After 23 days of fermentation, the fermented beverage has a total flavonoid content up to 0.5 mg/mL to 2.0 mg/mL, which is beneficial to human health, a pH of 3.2 to 3.8 and a total titratable acidity of up to 12 mg/mL to 18 mg/mL. The fermented beverage requires a short fermentation cycle, which is beneficial to industrial production.

Abstract: A method for preparing a sustained release antibacterial film includes: (1) mixing a cyclodextrin metal-organic framework (CD-MOF) material with a silver nitrate short-chain alcohol solution to prepare an intermediate material A; (2) mixing the intermediate material A with a caffeic acid short-chain alcohol solution to prepare an intermediate material B; (3) dispersing the intermediate material B in a solvent, adding a polydimethylsiloxane main agent and a PDMS auxiliary agent in sequence, and mixing well by stirring to obtain a casting solution; and (4) coating the casting solution on a film support material, conducting vacuum drying, and peeling off a film from the film support material to obtain the sustained-release antibacterial film. A mixed matrix-based film material in which the CD-MOF and the PDMS are physically blended is prepared based on CD-MOF and PDMS for the first time, and may be used in application researches of food, environment and other fields.

Abstract: The disclosure discloses a method to elevate the amount of steviol glycosides remained in the recrystallization mother liquor of Stevia extract, and belongs to the field of modification and extraction of natural compounds. The disclosure adopts lactase for highly specific catalytic hydrolysis of stevioside to convert stevioside into rubusoside which usually can only be obtained from leaves of Rubus suavissimus S. Lee; and meanwhile, after stevioside in the mother liquor glycosides is converted, since the polarity difference between rubusoside and rebaudioside A is far larger than the polarity difference between stevioside and rebaudioside A, rebaudioside A is more easily separated out of the mother liquor glycosides. A technology provided by the disclosure is also applicable to value increment of mixed steviol glycosides containing stevioside and is beneficial for separating out rebaudioside A from the mixed steviol glycosides.

Abstract: A method for promoting the rapid precipitation of travertine crystals by algae is disclosed. Microalgae is added to a body of water having a calcium ion concentration of 100-500 mg/L and stirred. The amount of microalgae is 0.1-8×108 cells/L. The invention adopts a method for promoting the rapid precipitation of travertine crystals by algae, which significantly improves the sedimentation rate of travertine crystals. At the same time, there are pseudomonas in the calcified water body of algae, which can be used for algae-lysing bacteria isolation and purification.

Abstract: The present disclosure discloses a salt-reduced fermentation method for high-salt dilute-state fermented soy sauce, and belongs to the technical field of fermentation engineering. The present disclosure separates and screens Weissella paramesenteroides JL-5 and Bacillus amyloliquefaciens JDF-2 which inhibit the growth of spoilage bacteria from low-salt soy sauce mash, and by changing the microbial fermentation process of high-salt dilute-state soy sauce, the strains obtained by screening are used for the fermentation of low-salt soy sauce mash. Without changing the flavor and quality of soy sauce, low-salt fermentation of the soy sauce is enabled to proceed normally, and the biogenic amine content is less than 100 mg L?1, and the number of spoilage bacteria in the soy sauce are reduced. The prepared low-salt soy sauce has an amino acid nitrogen content of greater than 1.2 g·100 mL?1, contents of various spoilage bacteria of less than 1.

Abstract: A novel direct-ink-writing (DIW) method for printing a strain gauge array circuit is disclosed. Firstly, the whole circuit is layered by a 3D printing method; a thin layer of insulating material is printed on the first circuit layer, instead of printing an insulating bridge, to form the second insulating layer; a part needing to contact the second insulating layer is not printed, but one through hole is reserved; the second circuit layer is then printed; the functional layer of the strain gauge is finally covered; the electrodes on the functional layer can contact the second circuit layer or can contact the first circuit layer through the through holes, which ensures that the functional layer contacts the two circuit layers, and also ensures the insulation in a cross part of a matrix circuit; the printing of the array strain gauge is effectively completed; and the stability of measurement is ensured.

Abstract: The present invention provides a knowledge reuse-based method and system for predicting a cell concentration in a fermentation process. The method includes: constructing a cell concentration soft sensor universal model in a fermentation process; acquiring and preprocessing process data of a fermentation stage A; determining a cell concentration soft sensor model of the fermentation stage A; designing a cell concentration online soft sensor of a fermentation stage B; and predicting a cell concentration of the fermentation stage B according to the cell concentration online soft sensor of the fermentation stage B. The present invention resolves the problems of weak generalization of a cell concentration soft sensor model and high costs of establishing models for fermentation stages separately, thereby improving the prediction accuracy of a cell concentration soft sensor.

Abstract: Disclosed are an agarase mutant with improved thermal stability and application thereof, belonging to the fields of genetic engineering technology and enzyme engineering. The present disclosure provides an agarase mutant, which is obtained by mutating the amino acid at the 86th site, the 373rd site, the 374th site, the 496th site, the 507th site, or the 747th site of agarase with an amino acid sequence as shown in SEQ ID NO. 1. The agarase mutant provided by the present disclosure improves the thermal stability and the hydrolytic activity of the agarase. Compared with the wild type enzyme, the mutant enzyme shows excellent heat resistance and can be industrially used at a relatively high temperature, so that the utilization rate of agar raw materials and the yield of oligosaccharides from agar are improved, and the mutant enzyme has a good industrial application prospect.

Abstract: The disclosure discloses an alginate lyase and application thereof, and belongs to the technical field of biology. The alginate lyase provided by the disclosure has high degradation activity, and the enzyme activity reaches 65 U/mg; the alginate lyase is stable in nature, and the enzyme activity remains 98% or higher of the initial enzyme activity after storage at 4° C. for 18 months; and the alginate lyase has high product specificity. The disclosure uses E. coli as a host to express the alginate lyase derived from V. natriegens, the obtained recombinant E. coli can produce the alginate lyase secreted extracellularly in a conventional LB medium without adding an induction substrate sodium alginate, so the downstream processing technology of protein is simplified, and the disclosure has great industrial application potential.

Abstract: This application relates to a yarn carrier device with a yarn tension control function for a flat knitting machine, and a yarn tension control method. At the moment a carriage is connected to a yarn carrier device, a fixing frame carrying a bobbin ascends or descends on sliding rails installed on the yarn carrier device. The ascending or descending speed, time and distance of the fixing frame can be calculated directly according to a yarn carrier stop, the quantity of an unknitted portion of the yarn and the speed of the carriage. When the carriage contacts a yarn carrier, the fixing frame is controlled to ascend along the sliding rails. When the carriage drives the yarn carrier to enter a knitting area, the fixing frame is controlled to descend.

Abstract: The present disclosure provides a preparation method of eucalyptol emulsion and application thereof in biopesticides, belonging to the field of emulsion preparation. The method for preparing the eucalyptol emulsion includes the steps of: (1) adding eucalyptol into a cyclodextrin solution to obtain a mixed solution; (2) evenly mixing an emulsifier and a co-emulsifier to obtain a mixed solution; (3) adding the solution obtained in step (2) and water into the solution obtained in step (1), emulsifying to obtain the eucalyptol emulsion. In steps (1), (2) and (3), the mass percents of substances from which the eucalyptol emulsion is prepared are as follows: 0.5%-1% of cyclodextrin, 2.5%-25% of the eucalyptol, 1.25%-15% of the emulsifier, 0.3%-3.75% of the co-emulsifier and 55.3%-95.5% of the water, and the sum thereof is 100%. The emulsion is extremely stable without stratification after being placed at 20° C., 40° C. and 60° C. for 30 days and also has a good insecticidal effect.

Abstract: The present disclosure discloses a high-temperature fast-curing starch-based adhesive for particleboards and a preparation method thereof, belonging to the technical field of adhesive preparation. The low viscosity of the starch-based adhesive is ensured by selecting a crosslinking monomer which does not self-crosslink in a reaction process in the present disclosure, and a binary crosslinking agent matched with the crosslinking monomer is added before use to be quickly crosslinked with the crosslinking monomer at high temperature so as to ensure better thermosetting property and water resistance as well as faster curing speed of the starch-based adhesive at the same time, which meets the requirements of the particleboards for the adhesive, solves the problem of long curing time of the existing starch-based adhesives at high temperatures, further shortens the curing time of the starch-based adhesives to about 60 s, and improves the production efficiency of the particleboards.

Abstract: The disclosure discloses stabilized rice aleurone and a preparation method, using the technology of classifying a rice aleurone layer by a tangential injection airflow impeller classifier combined with an ultrasonic vibrating screen for the first time, and simultaneously uses steam treatment coupled with thermal enzyme inactivation by drum drying as a stabilization method to prepare the stabilized rice aleurone for the first time. The drum drying equipment realizes the two purposes of enzyme inactivation and drying, and the method has the advantages of short process flow, simple equipment, high utilization rate, small floor space, low investment, low energy consumption and no pollution, and is suitable for industrial promotion. The stabilized rice aleurone has high nutritional value, is rich in functional ingredients, and can be used as a raw material for functional food development in industrial production and food applications.

Abstract: The disclosure discloses an ultrasonic-assisted pretreatment method for extraction of multiple steroid hormones in a sediment, including the following steps: (1) lyophilizing the sediment, grinding the sediment, and passing the ground sediment through a 40-60-mesh sieve; (2) placing the sample obtained in step (1) in a container; (3) adding an extractant to the container in step (2), shaking the mixture for 15 s-30 s, centrifuging the mixture to collect an supernatant after ultrasonication, and repeating extraction three times; where the extractants used in the three times of extraction are two of methanol, acetonitrile and acetone; and (4) after mixing the supernatants of the three times of extraction obtained in step (3), concentrating the mixture under a nitrogen flow at 20-30° C., passing the concentrated mixture through a filter, and performing detection. By using the method of the disclosure, the maximum recovery can be up to 100%.

Abstract: The invention provides a recombinant Escherichia coli strain for producing succinic acid and a construction method thereof. The by-product encoding genes in the E. coli strain FMME-N-2 are knocked out to obtain the E. coli strain FMME-N-5 (?focA-pflB-?ldhA-?pta-ackA); and the phosphoenolpyruvate carboxykinase pck derived from Actinobacillus succinogenes and the phosphite dehydrogenase ptxD derived from Pseudomonas stutzeri were overexpressed. The constructed plasmid pTrcHisA-pck-ptxD was introduced into the expression host E. coli FMME-N-5 (?focA-pflB-?ldhA-?pta-ackA), and the cells were screened in a plate containing ampicillin, to obtain an engineered strain E. coli FMME-N-5 (?focA-pflB-?ldhA-?pta-ackA)-pck-ptxD that can efficiently produce succinic acid. After fermentation by a two-stage fermentation strategy, the production of succinic acid reaches 137 g/L, the yield of succinic acid is up to 1 g/g glucose, and the space time yield is 1.

Abstract: The present invention discloses an iterative learning control (ILC) method for a multi-particle vehicle platoon driving system, and relates to the field of ILC. The method includes: firstly, discretizing a multi-particle train dynamic equation using a finite difference method to obtain a partial recurrence equation, and then transforming the partial recurrence equation into a spatially interconnected system model; secondly, transforming the spatially interconnected system model into an equivalent one-dimensional dynamic model using a lifting technology, and in order to compensate input delay, designing an ILC law based on a state observer; and thirdly, transforming a controlled object into an equivalent discrete repetitive process according to the ILC law, and converting a controller combination problem into a linear matrix inequality based on stability analysis of the repetitive process.

Abstract: The disclosure discloses an irreversible covalent organic framework for efficient and selective gold recovery and a preparation method thereof, and belongs to the technical field of precious metal recovery from an aqueous solution. In the disclosure, metal trifluoromethanesulfonate is used as a catalyst, and a solvothermal method is used to prepare a mother covalent organic framework, and then the corresponding structural unit is used to perform an exchange reaction to prepare an irreversible amide-linked covalent organic framework material. The disclosure solves the problem of preparation of high-stability irreversible covalent organic framework, the introduced amide bond gives the covalent organic framework the ability to quickly and selectively recover precious metal gold from an aqueous solution, and the covalent organic framework can be used repeatedly.