Abstract: Disclosed is an Aspergillus niger seed continuous culture method, comprising the steps of: (1) at a startup stage, Aspergillus niger spores are inoculated into a seed culture medium to obtain a seed liquid; (2) at a seed continuous culture stage, continuous dispersion treatment is performed on the seed liquid obtained in step (1), continuous culture is performed on the seed liquid obtained by dispersion, and meanwhile, a fresh seed feed medium is replenished; and (3) at a stop stage, the replenishment of the fresh seed feed medium and the dispersion treatment are stopped, continuous culture is performed to obtain a seed liquid, and then the seed liquid is transferred into the fermentation medium for fermentation culture. The method according to the present invention makes breakthrough to solve problems that multi-cellular filamentous bacteria grow slowly and mycelium pellets are easy to lose in continuous culture, thus fully achieving seed continuous culture.

Abstract: The disclosure discloses a method for producing jasmonic acid by solid state fermentation of microorganisms, and belongs to the field of biotechnology. Lasiodiplodia sp. is inoculated in a solid state fermentation medium containing wheat for static fermentation for 6-15 days, and then, jasmonic acid of 4128 mg/kg wheat can be produced. The method provides a feasibility for extension of production of jasmonic acid by fermentation of microorganisms.

Abstract: The present disclosure discloses a multistage nanoreactor catalyst and preparation and application thereof, belonging to the technical field of synthesis gas conversion. The catalyst consists of a core of an iron-based Fischer-Tropsch catalyst, a transition layer of a porous oxide or porous carbon material, and a shell layer of a molecular sieve having an aromatization function. The molecular sieve of the shell layer can be further modified by a metal element or a non-metal element, and the outer surface of the molecular sieve is further modified by a silicon-oxygen compound to adjust the acidic site on the outer surface and the aperture of the molecular sieve, thereby inhibiting the formation of heavy aromatic hydrocarbons. According to the disclosure, the shell layer molecular sieve with a transition layer and a shell layer containing or not containing auxiliaries, and with or without surface modification can be prepared by the iron-based Fischer-Tropsch catalyst through multiple steps.

Abstract: The disclosure discloses a multi-response cellulose nanocrystals-composite film and a preparation method thereof, belonging to the technical field of functional materials. The multi-response cellulose nanocrystals-composite film of the disclosure includes cellulose nanocrystals, a deep eutectic solvent and anthocyanins. The deep eutectic solvent is composed of choline chloride and biological sugar. A mass ratio of the choline chloride to the biological sugar is 1-20:1-6. A mass ratio of the cellulose nanocrystals to the deep eutectic solvent to the anthocyanins is 10:0-10:1, and the amount of the deep eutectic solvent is not 0. The preparation method of the composite film of the disclosure is simple and easy to operate, and has the advantages of mild conditions and short time consumption. The multi-response film prepared in the disclosure has humidity and pH detection functions, has the advantages of good flexibility, good reversibility, excellent stability, etc.

Abstract: The disclosure discloses Bacteroides ovatus for relieving endotoxin infection and application thereof, and belongs to the technical field of microorganisms. The Bacteroides ovatus CCFM1021 of the disclosure has low immunogenicity, and can reduce the content of pro-inflammatory factors and increase the concentration of anti-inflammatory factors in the blood of mice infected with endotoxin, up-regulate the number of Foxp3+ regulatory T cells and stabilize the composition of the intestinal microbiota. A pharmaceutical composition of the Bacteroides ovatus CCFM1021 for relieving endotoxin infection has broad application prospects.

Abstract: The present invention provides a method for preparing double-layered bursting beads with milk tea flavor comprising: preparing inner and/or outer shell forming solutions for inner and/or outer shells of the bursting beads; preparing inner and/or outer core material solutions for inner and/or outer core materials of the bursting beads; preparing inner and/or outer shell curing solutions for inner and/or outer shells of the bursting beads; adding the inner core material solution into the inner shell forming solution, incubating, curing in the inner shell curing solution, and filtering to obtain inner bursting beads; and adding the inner bursting beads and the outer core material solution into the outer shell forming solution, and curing in the outer shell curing solution to obtain the double-layered bursting beads.

Abstract: Disclosed is a real-time object detection method deployed on a platform with limited computing resources, which belongs to the field of deep learning and image processing. In the present invention, YOLO-v3-tiny neural network is improved, Tinier-YOLO reserves the front five convolutional layers and pooling layers of YOLO-v3-tiny and makes prediction at two different scales. Fire modules in SqueezeNet, 1×1 bottleneck layers, and dense connection are introduced, so that the structure is used to achieve smaller, faster, and more lightweight network that can be run in real time on an embedded AI platform. The model size of Tinier-YOLO in the present invention is only 7.9 MB, which is only ¼ of 34.9 MB of YOLO-v3-tiny, and ? of YOLO-v2-tiny. The reduction in the model size of Tinier-YOLO does not affect real-time performance and accuracy of Tinier-YOLO. Real-time performance of Tinier-YOLO in the present invention is 21.8% higher than that of YOLO-v3-tiny and 70.8% higher than that of YOLO-v2-tiny.

Abstract: The present invention relates to a pneumatic glue gun including tank components which contain a glue hose and an injector head; pressure generating units which contain a shell, an extruder, a slidable piston and a push/pull rod; and a drive component connected with the extruder. The tank components are fixed on the shell through a open end of the glue hose and faced the interior space of the shell. The drive component increases the atmospheric pressure between the extruder and the piston by driving the movement of the extruder in the shell, and the piston slides in the glue hose through the increased atmospheric pressure. The glue of the present invention does not need to be replaced, and the glue can be replenished at any time, so the work of replacing the tank is eliminated, and no waste of resources is caused.

Abstract: The present disclosure discloses an amylopectin-based cyclic glucan and the processing method for the same, and belongs to the technical field of food processing. The present disclosure uses a starch as a raw material, prepares an amylopectin-based cyclic glucan through sugar chain degradation grading and glycosidase-catalyzed trans-glycoside technology, and can be used as a steady-state carrier material for food active factors. The method of the disclosure has advantages of green environmental protection, high processing yield and low cost. The prepared product has high branching degree, special large ring structure and good water solubility, and can be used for steady-state delivery and active protection of natural functional substances, involving nutritional food, medicine, daily chemicals and other fields.

Abstract: The invention discloses a preparation method of a catechol group modified biomacromolecular scaffold material, comprising: grafting a catechol-containing compound by amidation to obtain modified biomacromolecules; then, allowing dopamine to perform oxidized self-polymerization in a weakly alkaline buffer solution to form polydopamine (PDA) particles with a uniform particle size; next, forming a scaffold which has three cross-linking structures, namely modified biomacromolecules, modified biomacromolecules/PDA, and biomacromolecules/PDA, through interaction between catechol groups, interaction between catechol groups and PDA particles, and interaction between macromolecules and PDA particles in the modified macromolecules respectively; and cross-linking the scaffold with calcium ions, adipic dihydrazide or genipin to further adjust the degree of cross-linking and porosity of the scaffold.

Abstract: The disclosure discloses a recombinant Bacillus subtilis engineered bacterium capable of efficiently expressing keratinase, and belongs to the technical fields of genetic engineering and fermentation engineering. The disclosure successfully constructs a genetically engineered bacterium B. subtilis WB600-pP43NMK-ker capable of efficiently expressing keratinase by using a keratinase gene from Bacillus licheniformis (BBE11-1) as a target gene, pP43NMK as an expression vector and B. subtilis WB600 as an expression host; and meanwhile, the disclosure conducts in-depth research on fermentation media and fermentation conditions when the engineered bacteria are configured as a production strain to produce keratinase to obtain a fermentation medium capable of increasing a yield of keratinase and an optimum process for fermentation production of keratinase.

Abstract: The present invention discloses a preparation method of a cotton bast fiber and chitosan composite non-woven fabric for filtration and belongs to the technical field of fiber materials. The preparation method adopts steam flash explosion for pretreatment to separate cotton bast first, then a papermaking method is used for forming, finally chitosan is added to enhance the mechanical performance and improve the filtration efficiency, and a technological process is simple, environmentally friendly and suitable for industrial production. The non-woven fabric adopts lignin-rich cotton bast fibers and chitosan as raw materials, so that the non-woven fabric has good antibacterial performance. The cotton bast and chitosan used in the preparation method are both bio-based materials which can be degraded in the natural environment after use, so that burdens caused to the environment are reduced.

Abstract: The disclosure discloses a method for separating phenolic acid glucoside compounds from Diaphragma juglandis Fructus, belonging to the technical field of food. The method includes the following steps: (1) extraction: crushing Diaphragma juglandis Fructus, and then extracting by using ethanol to obtain walnut ethanol extract; and (2) separation: performing column chromatography on the walnut ethanol extract obtained in step (1), sequentially eluting by using ethanol of different concentrations, purifying and eluting repeatedly, and performing separation to obtain phenolic acid glucoside compounds. The phenolic acid glucoside compounds are 2-hydroxyl-5-methoxylphenyl-O-?-D-glucopyranoside, 6-hydroxylnaphthyl-1,2-di-O-?-D-glucopyranoside and 2,6-dihydroxynaphthyl-O-?-D-glucopyranoside. Pharmacological experiments confirm that the three compounds have good antioxidant activity.

Abstract: The present invention provides a phospholipase D having an amino acid sequence as shown in SEQ ID NO. 1, and further provides a gene sequence encoding phospholipase D, which has a nucleotide sequence as shown in SEQ ID NO. 2. The present invention also provides a method for improving the expression level of phospholipase D by systematically engineering the expression elements. The method comprises screening and replacement of signal peptides, ribosome binding sites and promoters. The constructed recombinant plasmid is transformed into a host cell, and the recombinant strain is capable of successfully expressing phospholipase D. The phospholipase D of the present invention has a good phosphatidyl transferring ability, and can be used for synthesizing the product phosphatidylserine with lecithin and L-serine as substrates. The recombinant strain has good stability of enzyme activity and short fermentation period, which lays the foundation for large-scale industrial production.

Abstract: The disclosure discloses a probiotic mixed preparation with anti-influenza ability and application thereof, and belongs to the technical fields of microorganisms and medicines. The probiotic mixed preparation has anti-influenza effects, which are embodied in: (1) significantly reducing the degree of weight loss of influenza mice; (2) significantly improving the blood indexes of the influenza mice; (3) significantly improving the inflammation status of respiratory tract infections in the influenza mice; (4) significantly reducing the viral load in the lungs of the influenza mice (i.e., significantly inhibiting the replication and multiplication of influenza viruses in the influenza mice); and (5) significantly increasing the expression quantity of antiviral protein MxA in the lungs of the influenza mice. Therefore, the probiotic mixed preparation has great application prospects in preparation of a product for preventing and/or treating atopic dermatitis and even preventing and/or treating influenza.

Abstract: The present disclosure discloses a genetically engineered strain, belonging to the technical field of bioengineering. L-amino acid oxidase genes, ?-keto acid decarboxylase genes, alcohol dehydrogenase genes, and enzyme genes capable of reducing NAD(P) to NAD(P)H are introduced into the genetically engineered strain of the present disclosure. The present disclosure further discloses a construction method and application of a recombinant Escherichia coli genetically engineered strain. When being applied to the biosynthesis of phenylethanoids, the method of the present disclosure has the characteristics of simple operation, low cost, and high synthesis efficiency and optical purity of the product, and has good industrialization prospects.

Abstract: An automatic fabric folding device has a rotating mechanism, an automatic folding mechanism and a crease generating mechanism. The automatic folding mechanism is located below the finger cylinder on one side of the rotating mechanism, and the finger cylinder on the other side of the automatic folding mechanism is fixed with a crease generating mechanism. The two rotating cylinders are central symmetrical with the rotating arm. This accurately controls the bending and folding of the fabric sample through the automatic control technology, and realizes the automatic detection of the fabric crease recovery process. By mechanical and numerical control technology, the parts to be measured are effectively prevented from interference by human factors, and the detection accuracy is improved.

Abstract: The present invention provides an enzymatic method for producing a fatty acid bornyl ester including using borneol and a fatty acid as a substrate for reaction and adding a lipase in a solvent system or a solvent-free system to catalyze the esterification reaction for a period of time to obtain fatty acid bornyl ester. The present method preferably uses fatty acids or their derivatives as acyl donors to prepare fatty acid bornyl esters. By utilizing the characteristics of the substrate, the synthesis process is simple; the reaction efficiency is high; and the content of fatty acid bornyl ester is up to 97%.

Abstract: A multi-copolymer, methods for producing the same and using thereof in wastewater treatment application are provided. The method includes pre-mixing by mass 10-30 parts by mass of alkenyl succinic anhydrides, 10-30 parts of vinylbenzyltrimethylammonium chloride, and 10-30 parts of dimethylaminoethyl methacrylate with water to form a first mixture; reacting the first mixture comprising passing thereof through inert gases to provide reflux protection, and adding by mass 10-30 parts of vinyl acetate and 10-30 parts of acrylamide to form a second mixture, followed by heating the second mixture and adding an initiator therein to initiate the reaction.

Abstract: A method for preparing Cordyceps militaris nano-composite powder with protein-polysaccharide complex coacervation stability. The method includes sorting, drying, crushing suspending Cordyceps militaris ultrafine powder, emulsifying primarily, re-emulsifying, adjusting pH value and conducting spray drying. The emulsion stability after primary emulsification is not high, so re-emulsification technology is increased, and lactalbumin with higher emulsifying property and higher nutritional value are adopted as enhanced emulsifier, thus enhancing stability of an emulsifying system. The network envelopment stability of polysaccharide colloidal xanthan gum or kudzu powder is used to consolidate the stability of the emulsifying system, and a Pickering emulsifying system with multilayer of solid particle stability is constructed.