Abstract: The invention concerns the targeted genomic modification of a plant cell, preferably a meristem cell. More in particular, the invention pertains to a vector expressing a coding RNA, wherein the coding RNA comprises a sequence encoding a CRISPR-nuclease and a mobile element, wherein the mobile element enables intercellular translocation of the coding RNA, preferably intercellular translocation to a meristem cell. The invention further concerns an editing RNA comprising the coding RNA and further comprising a guide RNA.

Abstract: Stevia varieties with a high content of RebM, are disclosed Further provided are methods for producing Stevia plants having a high RebM content by negatively regulating certain genes selecting the resulting plants, and breeding with such plants to confer such desirable Reb M phenotypes to plant progeny.

Abstract: The present invention provides for a method for producing an inbred plant comprising a first and second trait of interest in the L1-shoot meristem layer for use in producing a periclinal chimera plant, the inbred plant thus obtained, the use of said inbred plant for producing said periclinal chimera plant, a method for producing a periclinal chimera plant using said inbred plant, a periclinal chimera plant thus obtained, the use of said periclinal chimera plant in producing plant product and the plant product thus obtained.

Abstract: The present invention relates to genetic elements comprising powdery mildew-conferring QTLs derived from a plant of the species Cucumis melo, or powdery mildew-conferring part or variant thereof. The invention also relates to markers for identification of said QTLs, use thereof and methods for producing plants with increased resistance to powdery mildew and the plants thus obtained.

Abstract: The invention relates to a kit of parts for detecting one or more polymorphisms in a plurality of target nucleotide sequences in a plurality of samples, wherein the kit of parts comprises (i) a first probe and a second probe, wherein the first probe comprises a first target specific section and a first tag section that is non-complementary to the target nucleotide sequence and that comprises a first universal primer binding site, wherein the second probe comprises a second target specific section and a second tag section that is non-complementary to the target nucleotide sequence; and (ii) a first primer and optionally a second primer wherein the first primer comprises a sample-specific identifier sequence and wherein the first primer can hybridize to the first universal primer binding site.

Abstract: The current invention pertains to a method for sequencing of a target nucleic acid fragment from a nucleic acid sample, comprising the steps of cleaving the nucleic acid sample with a first and a second RNA guided or DNA guided endonuclease complex, preferably a first and a second gRNA-CAS complex, thereby generating the target nucleic acid fragment and at least one non-target nucleic acid fragment. The generated fragments are subsequently contacted with an exonuclease, wherein the exonuclease digests only the non-target nucleic acid fragments. Subsequently said target nucleic acid fragment is sequenced using nanopore selective sequencing. The invention further pertains to the use of the enriched target nucleic acid fragments for nanopore selective sequencing the target nucleic acid fragment.

Abstract: The invention pertains to a method for labelling a target nucleic acid fragment using a combination of a site-specific nuclease and a reverse transcriptase. The labelling results in the addition of a specific nucleotide sequence to at least one free 3?-end of the target nucleic acid fragment. The invention further relates to a method for determining the sequence of the target nucleic acid fragment as well as construct and kit for use in the method of the invention.

Abstract: Stevia varieties with a high content of RebM, are disclosed Further provided are methods for producing Stevia plants having a high RebM content by negatively regulating certain genes selecting the resulting plants, and breeding with such plants to confer such desirable Reb M phenotypes to plant progeny.

Abstract: The present invention relates to genetic elements comprising powdery mildew-conferring QTLs derived from a plant of the species Cucumis melo, or powdery mildew-conferring part or variant thereof. The invention also relates to markers for identification of said QTLs, use thereof and methods for producing plants with increased resistance to powdery mildew and the plants thus obtained.

Abstract: Efficient methods are disclosed for the high throughput identification of mutations in genes in members of mutagenized populations. The methods comprise DNA isolation, pooling, amplification, creation of libraries, high throughput sequencing of libraries, preferably by sequencing-by-synthesis technologies, identification of mutations and identification of the member of the population carrying the mutation and identification of the mutation.

Abstract: It was found that plants with loss of functional Msi2 protein due to a nucleotide polymorphism resulting in the introduction of a premature stop codon in the Msi2 protein, are able to induce haploid offspring after a cross to or with a wild type plant comprising a functional Msi2 protein. The invention relates to generation of haploid and doubled haploid plants.

Abstract: A plant selection apparatus has a storage means for storing a plurality of image datasets of plants and associated plant information including at least one of genotype information of the plants, phenotype information of the plants, and pedigree information of the plants. A selection unit preselects a subset of the plants. A display device displays the image datasets of the subset of the plants. A tracking unit generates observation information including information on which of the subset of the plants is observed, and the storage means is configured for storing the observation information. A training unit trains a classifier based on the observation information, the input selection, and the plant information including said at least one of the genotype information, the phenotype information, and the pedigree information.

Abstract: The present invention provides methods for the production of plant inbred seed, or for the production of plant hybrid seed, wherein the seed obtained exhibits altered and/or improved germination properties, in particular improved germination properties such as enhanced seed germination rate, enhanced seed germination capacity and/or enhanced seedling fresh weight. The present invention also provides for the use of periclinal chimera plants for improving germination properties of seed.

Abstract: Disclosed is a new method of providing plant cells with a targeted alteration in a DNA molecule. The method comprises contacting a population of plant cells comprising a DNA molecule, the DNA molecule having a target sequence, with an aqueous medium, wherein the aqueous medium comprises a CRISPR associated protein (CAS protein) or a CAS-like protein, and a CRISPR-Cas system guide RNA that hybridizes with the target sequence, and wherein the aqueous medium comprises polyethylene glycol (PEG), but needs to be substantially free of glycerol.

Abstract: The invention provides nucleotide sequences and amino acid sequences of the Dip gene as well as (functional) homologues, fragments and variants thereof, which provides diplospory as a part of apomixis. Also diplospory plants and methods for making these are provided, as are methods of using these, and methods of making apomictic seed.

Abstract: A system for monitoring plants comprises an input unit, an output unit, and a processor that provides (201) image data (107) for a plurality of plants, each image data being associated with an individual plant. It associates (204) the image data (107) of each plant with corresponding plant characteristic data (109). It selects (206) a subset of the plants based on the plant characteristic data (109). It generates (207) a plurality of computer graphics objects corresponding to the selected plants. It applies (208) the image data (107) of each selected plant in form of a computer graphics texture to the corresponding computer graphics object. It determines (209) a position of each computer graphics object in a three-dimensional space of a computer graphics scene. It creates (210) a computer graphics rendering of the scene. It displays (211) the computer graphics rendering.

Abstract: The invention provides the nucleotide sequence and amino acid sequences of the parthenogenesis gene of Taraxacum as well as (functional) homologues, fragments and variants thereof, which provides parthenogenesis as a part of apomixis. Also parthenogenetic plants and methods for making these are provided, as are molecular markers and methods of using these.

Abstract: Stevia varieties with a high content of RebM, are disclosed Further provided are methods for producing Stevia plants having a high RebM content by negatively regulating certain genes selecting the resulting plants, and breeding with such plants to confer such desirable Reb M phenotypes to plant progeny.

Abstract: It was found that plants comprising modified CENPC protein comprising one or more active mutations which affect the functioning of CENPC protein yet allow plants expressing said modified CENPC protein to be viable, are able to induce haploid offspring after a cross to or with a wild type plant comprising a endogenous CENPC protein. The invention relates to generation of haploid and doubled haploid plants.

Abstract: The present invention provides methods for the production of plant inbred seed, or for the production of plant hybrid seed, wherein the seed obtained exhibits altered and/or improved germination properties, in particular improved germination properties such as enhanced seed germination rate, enhanced seed germination capacity and/or enhanced seedling fresh weight. The present invention also provides for the use of periclinal chimera plants for improving germination properties of seed.