Patents Assigned to Keygene, N. V.
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Publication number: 20190225657Abstract: The present disclosure provides a modified CenH3 protein that, when present in a plant, allows the plant to be used as a haploid inducer line for plant breeding purposes. Polynucleotides encoding such modified CenH3 proteins, chimeric genes and vectors comprising such polynucleotides, host cells, and plants comprising such polynucleotides, chimeric genes or vectors are also provided. Additionally, methods for making such plants as well as methods for producing haploid or doubled haploid plants using such plants are disclosed.Type: ApplicationFiled: May 19, 2017Publication date: July 25, 2019Applicant: Keygene N.V.Inventors: Rik Hubertus Martinus OP DEN CAMP, Peter Johannes VAN DIJK, Anthony GALLARD
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Patent number: 10316364Abstract: The present invention relates to a method for identifying the source of an amplicon, comprising: providing a plurality of pools of amplicons from different sources, wherein the amplicons from different sources are present in more than one pool, and wherein the amplicons in each pool are tagged with a unique pool-specific identifier; sequencing at least part of the amplicons that comprise the pool-specific identifiers; and assigning one or more of the amplicons to corresponding pools and/or sources using the pool-specific identifiers.Type: GrantFiled: September 18, 2017Date of Patent: June 11, 2019Assignee: KEYGENE N.V.Inventors: Michael Josephus Theresia Van Eijk, Taco Peter Jesse, Adrianus Johannes Van Tunen
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Patent number: 10308952Abstract: The present invention relates to a new method for increasing drought resistance of a plant. The method encompasses the impairment of the expression of a gene or genes in said plant. In comparison to a plant not manipulated to impair the expression of said gene(s), the plants display improved drought resistance. Also provided are plants and plant product that can be obtained by the method according to the invention.Type: GrantFiled: February 18, 2013Date of Patent: June 4, 2019Assignee: KEYGENE N.V.Inventors: Anne Deslattes Mays, Marieke Helena Adriana Van Hulten, Shital Anilkumar Dixit, Martin De Vos, Jesse David Munkvold, Matthew Vitabile Dileo
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Publication number: 20190153534Abstract: Efficient methods are disclosed for the high throughput identification of mutations in genes in members of mutagenized populations. The methods comprise DNA isolation, pooling, amplification, creation of libraries, high throughput sequencing of libraries, preferably by sequencing-by-synthesis technologies, identification of mutations and identification of the member of the population carrying the mutation and identification of the mutation.Type: ApplicationFiled: February 4, 2019Publication date: May 23, 2019Applicant: Keygene N.V.Inventors: Michael Josephus Theresia Van Eijk, Adrianus Johannes Van Tunen
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Publication number: 20190144938Abstract: The invention relates to a method for the high throughput discovery, detection and genotyping of one or more genetic markers in one or more samples, comprising the steps of restriction endonuclease digest of DNA, adaptor-ligation, optional pre-amplification, selective amplification, pooling of the amplified products, sequencing the libraries with sufficient redundancy, clustering followed by identification of the genetic markers within the library and/or between libraries and determination of (co-)dominant genotypes of the genetic markers.Type: ApplicationFiled: October 19, 2018Publication date: May 16, 2019Applicant: Keygene N.V.Inventors: Michael Josephus Theresia Van Eijk, Anker Preben Sørensen, Marco Gerardus Maria Van Schriek
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Publication number: 20190147977Abstract: The invention relates to a method for identifying one or more polymorphisms in nucleic acid samples, comprising: (a) performing a reproducible complexity reduction on a plurality of nucleic acid samples to provide a plurality of libraries of the nucleic acid samples comprising amplified fragments, wherein the reproducible complexity reduction comprises amplifying fragments of the nucleic acid samples using one or more primers to obtain the amplified fragments, and wherein the amplified fragments in each library comprise a unique identifier sequence to indicate origin of each library obtained by the reproducible complexity reduction; (b) combining the plurality of libraries to obtain a combined library and sequencing at least a portion of the combined library to obtain sequences; (c) aligning the sequences to obtain an alignment; and (d) identifying one or more polymorphisms in the plurality of nucleic acid samples.Type: ApplicationFiled: November 1, 2018Publication date: May 16, 2019Applicant: Keygene N.V.Inventors: Michael Josephus Theresia Van Eijk, Henricus Johannes Adam Van Der Poel
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Patent number: 10273500Abstract: The present invention relates to powdery mildew resistance providing genes of the Cucumis family, and especially Cucumis sativus, wherein said resistance is provided by impairment of the present genes. Further, the present invention relates plants comprising the present impaired resistance conferring genes and seeds, embryos or other propagation material thereof. Especially, the present invention relates to powdery mildew resistance conferring genes, wherein the amino acid sequence encoded by said resistance conferring gene is selected from the group consisting of SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID NO: 16, SEQ ID NO: 18, SEQ ID NO: 20, and SEQ ID NO: 22, and amino acid sequences with more than 70% identity, preferably more than 80% identity, more preferably more than 90% identity, and most preferably more than 95% identity.Type: GrantFiled: September 29, 2016Date of Patent: April 30, 2019Assignees: Enza Zaden Beheer B.V., Keygene N.V.Inventors: Paul Johan Diergaarde, Leonora Johanna Gertruda van Enckevort, Karin Ingeborg Posthuma, Marinus Willem Prins
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Patent number: 10235494Abstract: The invention relates to a method for identifying one or more polymorphisms in nucleic acid samples, comprising: (a) performing a reproducible complexity reduction on a plurality of nucleic acid samples to provide a plurality of libraries of the nucleic acid samples comprising amplified fragments, wherein the reproducible complexity reduction comprises amplifying fragments of the nucleic acid samples using one or more primers to obtain the amplified fragments, and wherein the amplified fragments in each library comprise a unique identifier sequence to indicate origin of each library obtained by the reproducible complexity reduction; (b) combining the plurality of libraries to obtain a combined library and sequencing at least a portion of the combined library to obtain sequences; (c) aligning the sequences to obtain an alignment; and (d) identifying one or more polymorphisms in the plurality of nucleic acid samples.Type: GrantFiled: April 24, 2018Date of Patent: March 19, 2019Assignee: Keygene N.V.Inventors: Michael Josephus Theresia Van Eijk, Henricus Johannes Adam Van Der Poel
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Patent number: 10233494Abstract: Efficient methods are disclosed for the high throughput identification of mutations in genes in members of mutagenized populations. The methods comprise DNA isolation, pooling, amplification, creation of libraries, high throughput sequencing of libraries, preferably by sequencing-by-synthesis technologies, identification of mutations and identification of the member of the population carrying the mutation and identification of the mutation.Type: GrantFiled: April 24, 2018Date of Patent: March 19, 2019Assignee: Keygene N.V.Inventors: Michael Josephus Theresia Van Eijk, Adrianus Johannes Van Tunen
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Publication number: 20190078117Abstract: It was found that plants comprising modified CENPC protein comprising one or more active mutations which affect the functioning of CENPC protein yet allow plants expressing said modified CENPC protein to be viable, are able to induce haploid offspring after a cross to or with a wild type plant comprising a endogenous CENPC protein. The invention relates to generation of haploid and doubled haploid plants.Type: ApplicationFiled: October 3, 2016Publication date: March 14, 2019Applicant: Keygene N.V.Inventors: Rik Hubertus Martinus OP DEN CAMP, Peter Johannes VAN DIJK, Anthony GALLARD
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Publication number: 20190029202Abstract: It was found that plants with loss of functional Msi2 protein due to a nucleotide polymorphism resulting in the introduction of a premature stop codon in the Msi2 protein, are able to induce haploid offspring after a cross to or with a wild type plant comprising a functional Msi2 protein. The invention relates to generation of haploid and doubled haploid plants.Type: ApplicationFiled: October 3, 2016Publication date: January 31, 2019Applicant: Keygene N.V.Inventors: Rik Hubertus Martinus OP DEN CAMP, Peter Johannes VAN DIJK, Anthony GALLARD
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Publication number: 20180319904Abstract: The present invention relates to a method for obtaining rubber from rubber-containing plant material, the method comprising the steps of subjecting a composition comprising rubber-containing plant material and a fermentation culture to conditions allowing anaerobic fermentation, wherein the composition preferably has a water content of less than 95 wt. %. The fermentation preferably does not involve movement of the rubber-containing plant material (or parts thereof), and preferably the fermentation is performed in the absence of oxygen ventilation. Additionally, it is preferred that the fermentation culture is cycled through the composition by percolation. A further step of the method involves obtaining the rubber from the composition.Type: ApplicationFiled: November 3, 2016Publication date: November 8, 2018Applicant: Keygene N.V.Inventors: Peter Johannes VAN DIJK, Rolf André MANK
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Patent number: 10106850Abstract: The invention relates to a method for the high throughput discovery, detection and genotyping of one or more genetic markers in one or more samples, comprising the steps of restriction endonuclease digest of DNA, adaptor-ligation, optional pre-amplification, selective amplification, pooling of the amplified products, sequencing the libraries with sufficient redundancy, clustering followed by identification of the genetic markers within the library and/or between libraries and determination of (co-)dominant genotypes of the genetic markers.Type: GrantFiled: August 22, 2017Date of Patent: October 23, 2018Assignee: Keygene N.V.Inventors: Michael Josephus Theresia Van Eijk, Anker Preben Sørensen, Marco Gerardus Maria Van Schriek
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Publication number: 20180291439Abstract: The present invention relates to a high throughput method for the identification and detection of molecular markers wherein restriction fragments are generated and suitable adaptors comprising (sample-specific) identifiers are ligated. The adapter-ligated restriction fragments may be selectively amplified with adaptor compatible primers carrying selective nucleotides at their 3? end. The amplified adapter-ligated restriction fragments are, at least partly, sequenced using high throughput sequencing methods and the sequence parts of the restriction fragments together with the sample-specific identifiers serve as molecular marker.Type: ApplicationFiled: June 5, 2018Publication date: October 11, 2018Applicant: Keygene N.V.Inventors: Michael Josephus Theresia Van Eijk, Rene Cornelis Josephus Hogers
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Patent number: 10093944Abstract: The present invention relates to powdery mildew resistance providing genes of the Cucumis family, and especially Cucumis melo, wherein said resistance is provided by impairment of the present genes. Further, the present invention relates plants comprising the present impaired resistance conferring genes and seeds, embryos or other propagation material thereof. Especially, the present invention relates to powdery mildew resistance conferring genes, wherein the amino acid sequence encoded by said resistance conferring gene is selected from the group consisting of SEQ ID No. 2, SEQ ID No. 4, SEQ ID No. 6, SEQ ID. No. 8, SEQ ID No. 10, SEQ ID No. 12 and SEQ ID No. 14, and amino acid sequences with more than 70% identity, preferably more than 80% identity, more preferably more than 90% identity, and most preferably more than 95% identity.Type: GrantFiled: June 1, 2017Date of Patent: October 9, 2018Assignees: Enza Zaden Beheer B.V., Keygene N.V.Inventors: Paul Johan Diergaarde, Leonora Johanna Gertruda van Enckevort, Karin Ingeborg Posthuma, Marinus Willem Prins
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Patent number: 10095832Abstract: The invention relates to a method for identifying one or more polymorphisms in nucleic acid samples, comprising: (a) performing a reproducible complexity reduction on a plurality of nucleic acid samples to provide a plurality of libraries of the nucleic acid samples comprising amplified fragments, wherein the reproducible complexity reduction comprises amplifying fragments of the nucleic acid samples using one or more primers to obtain the amplified fragments, and wherein the amplified fragments in each library comprise a unique identifier sequence to indicate origin of each library obtained by the reproducible complexity reduction; (b) combining the plurality of libraries to obtain a combined library and sequencing at least a portion of the combined library to obtain sequences; (c) aligning the sequences to obtain an alignment; and (d) identifying one or more polymorphisms in the plurality of nucleic acid samples.Type: GrantFiled: January 5, 2018Date of Patent: October 9, 2018Assignee: Keygene N.V.Inventors: Michael Josephus Theresia Van Eijk, Henricus Johannes Adam Van Der Poel
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Publication number: 20180271043Abstract: The present invention relates to a method for obtaining Taraxacum plants of considerable size and substantial rubber content, preferably by providing Taraxacum plants having a relatively large genome size and/or having a specific percentage of Taraxacum koksaghyz (TKS) derived genes and a specific percentage of Taraxacum officinale (TO) derived genes; selecting these plants for the presence or absence of certain markers; and subsequently selecting the plants on plant size, and on genome size.Type: ApplicationFiled: September 2, 2016Publication date: September 27, 2018Applicant: Keygene N.V.Inventors: Peter Johannes VAN DIJK, Anker Preben SØRENSEN
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Publication number: 20180245151Abstract: Efficient methods are disclosed for the high throughput identification of mutations in genes in members of mutagenized populations. The methods comprise DNA isolation, pooling, amplification, creation of libraries, high throughput sequencing of libraries, preferably by sequencing-by-synthesis technologies, identification of mutations and identification of the member of the population carrying the mutation and identification of the mutation.Type: ApplicationFiled: April 24, 2018Publication date: August 30, 2018Applicant: Keygene N.V.Inventors: Michael Josephus Theresia VAN EIJK, Adrianus Johannes VAN TUNEN
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Publication number: 20180247017Abstract: The invention relates to a method for identifying one or more polymorphisms in nucleic acid samples, comprising: (a) performing a reproducible complexity reduction on a plurality of nucleic acid samples to provide a plurality of libraries of the nucleic acid samples comprising amplified fragments, wherein the reproducible complexity reduction comprises amplifying fragments of the nucleic acid samples using one or more primers to obtain the amplified fragments, and wherein the amplified fragments in each library comprise a unique identifier sequence to indicate origin of each library obtained by the reproducible complexity reduction; (b) combining the plurality of libraries to obtain a combined library and sequencing at least a portion of the combined library to obtain sequences; (c) aligning the sequences to obtain an alignment; and (d) identifying one or more polymorphisms in the plurality of nucleic acid samples.Type: ApplicationFiled: April 24, 2018Publication date: August 30, 2018Applicant: Keygene N.V.Inventors: Michael Josephus Theresia VAN EIJK, Henricus Johannes Adam VAN DER POEL
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Publication number: 20180216122Abstract: The invention provides nucleotide sequences and amino acid sequences of the Dipgene as well as (functional) homologues, fragments and variants thereof, which provides diplospory as a part of apomixis. Also diplospory plants and methods for making these are provided, as are methods of using these, and methods of making apomictic seed.Type: ApplicationFiled: September 5, 2016Publication date: August 2, 2018Applicant: Keygene N.V.Inventors: Peter Johannes VAN DIJK, Diana RIGOLA, Marinus Willem PRINS, Adrianus Johannes VAN TUNEN