Abstract: The present invention provides methods for the production of plant inbred seed, or for the production of plant hybrid seed, wherein the seed obtained exhibits altered and/or improved germination properties, in particular improved germination properties such as enhanced seed germination rate, enhanced seed germination capacity and/or enhanced seedling fresh weight. The present invention also provides for the use of periclinal chimera plants for improving germination properties of seed.

Abstract: The present invention provides for a method for producing an inbred plant comprising a first and second trait of interest in the L1-shoot meristem layer for use in producing a periclinal chimera plant, the inbred plant thus obtained, the use of said inbred plant for producing said periclinal chimera plant, a method for producing a periclinal chimera plant using said inbred plant, a periclinal chimera plant thus obtained, the use of said periclinal chimera plant in producing plant product and the plant product thus obtained.

Abstract: Efficient methods are disclosed for the high throughput identification of mutations in genes in members of mutagenized populations. The methods comprise DNA isolation, pooling, amplification, creation of libraries, high throughput sequencing of libraries, preferably by sequencing-by-synthesis technologies, identification of mutations and identification of the member of the population carrying the mutation and identification of the mutation.

Abstract: A system for monitoring plants comprises an input unit, an output unit, and a processor that provides (201) image data (107) for a plurality of plants, each image data being associated with an individual plant. It associates (204) the image data (107) of each plant with corresponding plant characteristic data (109). It selects (206) a subset of the plants based on the plant characteristic data (109). It generates (207) a plurality of computer graphics objects corresponding to the selected plants. It applies (208) the image data (107) of each selected plant in form of a computer graphics texture to the corresponding computer graphics object. It determines (209) a position of each computer graphics object in a three-dimensional space of a computer graphics scene. It creates (210) a computer graphics rendering of the scene. It displays (211) the computer graphics rendering.

Abstract: Disclosed is a new method of providing plant cells with a targeted alteration in a DNA molecule. The method comprises contacting a population of plant cells comprising a DNA molecule, the DNA molecule having a target sequence, with an aqueous medium, wherein the aqueous medium comprises a CRISPR associated protein (CAS protein) or a CAS-like protein, and a CRISPR-Cas system guide RNA that hybridizes with the target sequence, and wherein the aqueous medium comprises polyethylene glycol (PEG), but needs to be substantially free of glycerol.

Abstract: The invention relates to a method for the detection of a target nucleotide sequence in a sample based on an oligonucleotide ligation assay wherein probes are used that contain (a combination of) sequence-based identifiers that can identify the sample and the target sequence (i.e. locus and/or allele combination) wherein after the ligation step, the ligated probes, or after amplification, the amplified ligated probes, are restricted using restriction enzymes to cut of part of the probes and continue with those parts (identifiers and target sequence) that contain the relevant information in the sequencing step.

Abstract: The present invention relates to a new method for increasing drought resistance of a plant. The method encompasses the impairment of the expression of a gene or genes in said plant. In comparison to a plant not manipulated to impair the expression of said gene(s), the plants display improved drought resistance. Also provided are plants and plant product that can be obtained by the method according to the invention.

Abstract: The present disclosure provides a modified CenH3 protein that, when present in a plant, allows the plant to be used as a haploid inducer line for plant breeding purposes. Polynucleotides encoding such modified CenH3 proteins, chimeric genes and vectors comprising such polynucleotides, host cells, and plants comprising such polynucleotides, chimeric genes or vectors are also provided. Additionally, methods for making such plants as well as methods for producing haploid or doubled haploid plants using such plants are disclosed.

Abstract: The present invention relates to a method for identifying the source of an amplicon, comprising: providing a plurality of pools of amplicons from different sources, wherein the amplicons from different sources are present in more than one pool, and wherein the amplicons in each pool are tagged with a unique pool-specific identifier; sequencing at least part of the amplicons that comprise the pool-specific identifiers; and assigning one or more of the amplicons to corresponding pools and/or sources using the pool-specific identifiers.

Abstract: The present invention relates to a new method for increasing drought resistance of a plant. The method encompasses the impairment of the expression of a gene or genes in said plant. In comparison to a plant not manipulated to impair the expression of said gene(s), the plants display improved drought resistance. Also provided are plants and plant product that can be obtained by the method according to the invention.

Abstract: Efficient methods are disclosed for the high throughput identification of mutations in genes in members of mutagenized populations. The methods comprise DNA isolation, pooling, amplification, creation of libraries, high throughput sequencing of libraries, preferably by sequencing-by-synthesis technologies, identification of mutations and identification of the member of the population carrying the mutation and identification of the mutation.

Abstract: The invention relates to a method for identifying one or more polymorphisms in nucleic acid samples, comprising: (a) performing a reproducible complexity reduction on a plurality of nucleic acid samples to provide a plurality of libraries of the nucleic acid samples comprising amplified fragments, wherein the reproducible complexity reduction comprises amplifying fragments of the nucleic acid samples using one or more primers to obtain the amplified fragments, and wherein the amplified fragments in each library comprise a unique identifier sequence to indicate origin of each library obtained by the reproducible complexity reduction; (b) combining the plurality of libraries to obtain a combined library and sequencing at least a portion of the combined library to obtain sequences; (c) aligning the sequences to obtain an alignment; and (d) identifying one or more polymorphisms in the plurality of nucleic acid samples.

Abstract: The invention relates to a method for the high throughput discovery, detection and genotyping of one or more genetic markers in one or more samples, comprising the steps of restriction endonuclease digest of DNA, adaptor-ligation, optional pre-amplification, selective amplification, pooling of the amplified products, sequencing the libraries with sufficient redundancy, clustering followed by identification of the genetic markers within the library and/or between libraries and determination of (co-)dominant genotypes of the genetic markers.

Abstract: The present invention relates to powdery mildew resistance providing genes of the Cucumis family, and especially Cucumis sativus, wherein said resistance is provided by impairment of the present genes. Further, the present invention relates plants comprising the present impaired resistance conferring genes and seeds, embryos or other propagation material thereof. Especially, the present invention relates to powdery mildew resistance conferring genes, wherein the amino acid sequence encoded by said resistance conferring gene is selected from the group consisting of SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID NO: 16, SEQ ID NO: 18, SEQ ID NO: 20, and SEQ ID NO: 22, and amino acid sequences with more than 70% identity, preferably more than 80% identity, more preferably more than 90% identity, and most preferably more than 95% identity.

Abstract: Efficient methods are disclosed for the high throughput identification of mutations in genes in members of mutagenized populations. The methods comprise DNA isolation, pooling, amplification, creation of libraries, high throughput sequencing of libraries, preferably by sequencing-by-synthesis technologies, identification of mutations and identification of the member of the population carrying the mutation and identification of the mutation.

Abstract: The invention relates to a method for identifying one or more polymorphisms in nucleic acid samples, comprising: (a) performing a reproducible complexity reduction on a plurality of nucleic acid samples to provide a plurality of libraries of the nucleic acid samples comprising amplified fragments, wherein the reproducible complexity reduction comprises amplifying fragments of the nucleic acid samples using one or more primers to obtain the amplified fragments, and wherein the amplified fragments in each library comprise a unique identifier sequence to indicate origin of each library obtained by the reproducible complexity reduction; (b) combining the plurality of libraries to obtain a combined library and sequencing at least a portion of the combined library to obtain sequences; (c) aligning the sequences to obtain an alignment; and (d) identifying one or more polymorphisms in the plurality of nucleic acid samples.

Abstract: It was found that plants comprising modified CENPC protein comprising one or more active mutations which affect the functioning of CENPC protein yet allow plants expressing said modified CENPC protein to be viable, are able to induce haploid offspring after a cross to or with a wild type plant comprising a endogenous CENPC protein. The invention relates to generation of haploid and doubled haploid plants.

Abstract: It was found that plants with loss of functional Msi2 protein due to a nucleotide polymorphism resulting in the introduction of a premature stop codon in the Msi2 protein, are able to induce haploid offspring after a cross to or with a wild type plant comprising a functional Msi2 protein. The invention relates to generation of haploid and doubled haploid plants.

Abstract: The present invention relates to a method for obtaining rubber from rubber-containing plant material, the method comprising the steps of subjecting a composition comprising rubber-containing plant material and a fermentation culture to conditions allowing anaerobic fermentation, wherein the composition preferably has a water content of less than 95 wt. %. The fermentation preferably does not involve movement of the rubber-containing plant material (or parts thereof), and preferably the fermentation is performed in the absence of oxygen ventilation. Additionally, it is preferred that the fermentation culture is cycled through the composition by percolation. A further step of the method involves obtaining the rubber from the composition.

Abstract: The invention relates to a method for the high throughput discovery, detection and genotyping of one or more genetic markers in one or more samples, comprising the steps of restriction endonuclease digest of DNA, adaptor-ligation, optional pre-amplification, selective amplification, pooling of the amplified products, sequencing the libraries with sufficient redundancy, clustering followed by identification of the genetic markers within the library and/or between libraries and determination of (co-)dominant genotypes of the genetic markers.