Patents Assigned to Keygene, N. V.
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Publication number: 20220033879Abstract: The current invention pertains to a method for the enrichment of a target nucleic acid fragment from a nucleic acid sample, comprising the steps of cleaving the nucleic acid sample with a first and a second RNA guided or DNA guided endonuclease complex, preferably a first and a second gRNA-CAS complex, thereby generating the target nucleic acid fragment and at least one non-target nucleic acid fragment. The generated fragments are subsequently contacted with an exonuclease, wherein the exonuclease digests only the non-target nucleic acid fragments. The invention further pertains to the use of the enriched target nucleic acid fragments for preparing an adapter ligated target nucleic acid fragment and for sequencing the target nucleic acid fragment.Type: ApplicationFiled: November 27, 2019Publication date: February 3, 2022Applicant: KEYGENE N.V.Inventors: Stefan John WHITE, René Cornelis Josephus HOGERS
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Patent number: 11236346Abstract: The invention provides nucleotide sequences and amino acid sequences of the Dipgene as well as (functional) homologues, fragments and variants thereof, which provides diplospory as a part of apomixis. Also diplospory plants and methods for making these are provided, as are methods of using these, and methods of making apomictic seed.Type: GrantFiled: September 5, 2016Date of Patent: February 1, 2022Assignee: KEYGENE N.V.Inventors: Peter Johannes Van Dijk, Diana Rigola, Marinus Willem Prins, Adrianus Johannes Van Tunen
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Publication number: 20220025445Abstract: The current invention pertains to a reliable method for determining the relative frequency of a sequence variant of interest in a nucleic acid sample derived from at least one polyploid cell, wherein the method uses a UMI to correct for any amplification biases. The invention further pertains to the use of a UMI for accurately determining the relative frequency of a sequence variant of interest in a nucleic acid sample derived from at least one polyploid cell.Type: ApplicationFiled: August 13, 2021Publication date: January 27, 2022Applicant: Keygene N.V.Inventors: Stefan John WHITE, René Cornelis Josephus HOGERS
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Publication number: 20220010321Abstract: The invention pertains to a method for targeted modification of DNA in a plant cell, comprising a step of contacting the DNA with an RNA-guided CRISPR-system nuclease complex, wherein the complex comprises a crRNA and a tracrRNA as separate molecules. The invention further pertains to said RNA-guided CRISPR-system nuclease complex for targeting of DNA in a plant cell and kits comprising the RNA-guided CRISPR-system nuclease complex or constructs encoding the same.Type: ApplicationFiled: November 1, 2019Publication date: January 13, 2022Applicant: KEYGENE N.V.Inventor: Franck Georges Paul LHUISSIER
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Publication number: 20210324393Abstract: Method for targeted alteration of a duplex acceptor DNA sequence in a plant cell protoplast, comprising combining the duplex acceptor DNA sequence with a donor mutagenic nucleobase, wherein the duplex acceptor DNA sequence contains a first DNA sequence and a second DNA sequence which is the complement of the first DNA sequence and wherein the donor mutagenic nucleobase comprises at least one mismatch with respect to the duplex acceptor DNA sequence to be altered, preferably with respect to the first DNA sequence, wherein the method further comprises a step of introducing the donor mutagenic nucleobase into the cell protoplasts using polyethylene glycol (PEG) mediated transformation and the use of PEG protoplast transformation for enhancing the rate of targeted mutagenesis.Type: ApplicationFiled: May 11, 2021Publication date: October 21, 2021Applicant: Keygene N.V.Inventors: Paul BUNDOCK, Michiel Theodoor Jan DE BOTH, Frank LHUISSIER
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Patent number: 11107551Abstract: The present invention provides a method for improving at least one phenotypic trait of interest in subsequent generation(s) of a population of individuals, preferably crop plants or cattle. Particularly, the method identifies the combination of at least three individuals that gives, upon subsequent intercrossing, the highest estimated probability of improving the at least one phenotypic trait of interest in the subsequent generation(s). Also provided is a computer-readable medium comprising instructions for performing the method.Type: GrantFiled: June 13, 2014Date of Patent: August 31, 2021Assignee: KEYGENE N.V.Inventors: Jacob Bernhard Buntjer, José Lúcio Lima Guerra, Timotheus Gerardus Doeswijk, Remco Van Berloo, Niek Bouman
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Publication number: 20210238612Abstract: Provided is a method for targeted modification of DNA in a plant cell using crRNA-guided MAD7-nuclease and compositions and kits for doing the same.Type: ApplicationFiled: July 12, 2019Publication date: August 5, 2021Applicant: Keygene N.V.Inventor: Paul BUNDOCK
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Patent number: 11060100Abstract: The invention is in the field of agriculture, in particular in the field of crop protection, more particularly in the field of providing nematode resistance to plants. A method for producing a plant having improved nematode resistance, particularly to root-knot nematodes and/or cyst nematodes, is disclosed, as well as a plant produced by such method.Type: GrantFiled: June 16, 2017Date of Patent: July 13, 2021Assignee: KEYGENE N.V.Inventors: Matthew Vitabile Dileo, Jesse David Munkvold, Martin De Vos, Anna Maria Tomczak, Sandra Goritschnig
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Publication number: 20210207213Abstract: The invention relates to a kit of parts for detecting one or more polymorphisms in a plurality of target nucleotide sequences in a plurality of samples, wherein the kit of parts comprises (i) a first probe and a second probe, wherein the first probe comprises a first target specific section and a first tag section that is non-complementary to the target nucleotide sequence and that comprises a first universal primer binding site, wherein the second probe comprises a second target specific section and a second tag section that is non-complementary to the target nucleotide sequence; and (ii) a first primer and optionally a second primer wherein the first primer comprises a sample-specific identifier sequence and wherein the first primer can hybridize to the first universal primer binding site.Type: ApplicationFiled: March 22, 2021Publication date: July 8, 2021Applicant: Keygene N.V.Inventors: Michael Josephus Theresia Van Eijk, Rene Cornelis Josephus Hogers
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Publication number: 20210202035Abstract: The invention relates to a method for identifying one or more polymorphisms in nucleic acid samples, comprising: (a) performing a reproducible complexity reduction on a plurality of nucleic acid samples to provide a plurality of libraries of the nucleic acid samples comprising amplified fragments, wherein the reproducible complexity reduction comprises amplifying fragments of the nucleic acid samples using one or more primers to obtain the amplified fragments, and wherein the amplified fragments in each library comprise a unique identifier sequence to indicate origin of each library obtained by the reproducible complexity reduction; (b) combining the plurality of libraries to obtain a combined library and sequencing at least a portion of the combined library to obtain sequences; (c) aligning the sequences to obtain an alignment; and (d) identifying one or more polymorphisms in the plurality of nucleic acid samples.Type: ApplicationFiled: March 11, 2021Publication date: July 1, 2021Applicant: KEYGENE N.V.Inventors: Michael Josephus Theresia VAN EIJK, Henricus Johannes Adam VAN DER POEL
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Publication number: 20210164021Abstract: The invention concerns a method for the production of oligonucleotides. The method of the invention uses a combination of amplification, restriction and affinity purification to produce high quality oligonucleotides. The invention further pertains to a nucleic acid precursor for use in the method of the invention, a solid support comprising said nucleic acid precursor and a kit for use in the method of the invention.Type: ApplicationFiled: December 10, 2020Publication date: June 3, 2021Applicant: Keygene N.V.Inventors: Rene Cornelis Josephus HOGERS, Maria Johanna BLEEKER, Michael Josephus Theresia VAN EIJK
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Patent number: 11008615Abstract: The invention relates to a kit for use in a method for detecting genetic variation in one or more members of a population, comprising (a) an adaptor adapted for ligation to a plurality of nucleic acid fragments and (b) a set of primers for PCR amplification having a 5?-end and a 3?-end, wherein at least one of the primers comprises one or more selective nucleotides at the 3? end, and wherein the adaptor and/or at least one of the primers comprises a sample-specific identifier sequence capable of indicating sample origin of an amplification product.Type: GrantFiled: July 19, 2019Date of Patent: May 18, 2021Assignee: KEYGENE N.V.Inventors: Michael Josephus Theresia Van Eijk, Anker Preben Sørensen, Marco Gerardus Maria Van Schriek
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Patent number: 11008579Abstract: Method for targeted alteration of a duplex acceptor DNA sequence in a plant cell protoplast, comprising combining the duplex acceptor DNA sequence with a donor mutagenic nucleobase, wherein the duplex acceptor DNA sequence contains a first DNA sequence and a second DNA sequence which is the complement of the first DNA sequence and wherein the donor mutagenic nucleobase comprises at least one mismatch with respect to the duplex acceptor DNA sequence to be altered, preferably with respect to the first DNA sequence, wherein the method further comprises a step of introducing the donor mutagenic nucleobase into the cell protoplasts using polyethylene glycol (PEG) mediated transformation and the use of PEG protoplast transformation for enhancing the rate of targeted mutagenesis.Type: GrantFiled: March 30, 2016Date of Patent: May 18, 2021Assignee: Keygene N.V.Inventors: Paul Bundock, Michiel Theodoor Jan De Both, Frank Lhuissier
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Patent number: 10995338Abstract: The current disclosure relates to the field of plants, in particular to the fields of plant breeding and plant genetics. More particular, the disclosure concerns inventive methodology that may be useful in improving plant properties. In particular the invention may be useful in removing linkage drag. Also provided are plant and plant parts obtained with the method disclosed herein.Type: GrantFiled: December 20, 2013Date of Patent: May 4, 2021Assignee: KEYGENE N.V.Inventors: Paul Bundock, Jeroen Stuurman
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Publication number: 20210123068Abstract: The current disclosure relates to the field of plants, in particular to the fields of plant breeding and plant genetics. More particular, the disclosure concerns inventive methodology that may be useful in improving plant properties. In particular the invention may be useful in removing linkage drag. Also provided are plant and plant parts obtained with the method disclosed herein.Type: ApplicationFiled: January 5, 2021Publication date: April 29, 2021Applicant: KEYGENE N.V.Inventors: Paul BUNDOCK, Jeroen STUURMAN
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Patent number: 10988807Abstract: The invention relates to a kit of parts for detecting one or more polymorphisms in a plurality of target nucleotide sequences in a plurality of samples, wherein the kit of parts comprises (i) a first probe and a second probe, wherein the first probe comprises a first target specific section and a first tag section that is non-complementary to the target nucleotide sequence and that comprises a first universal primer binding site, wherein the second probe comprises a second target specific section and a second tag section that is non-complementary to the target nucleotide sequence; and (ii) a first primer and optionally a second primer wherein the first primer comprises a sample-specific identifier sequence and wherein the first primer can hybridize to the first universal primer binding site.Type: GrantFiled: August 13, 2019Date of Patent: April 27, 2021Assignee: KEYGENE N.V.Inventors: Michael Josephus Theresia Van Eijk, René Cornelis Josephus Hogers
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Patent number: 10978175Abstract: The invention relates to a method for identifying one or more polymorphisms in nucleic acid samples, comprising: (a) performing a reproducible complexity reduction on a plurality of nucleic acid samples to provide a plurality of libraries of the nucleic acid samples comprising amplified fragments, wherein the reproducible complexity reduction comprises amplifying fragments of the nucleic acid samples using one or more primers to obtain the amplified fragments, and wherein the amplified fragments in each library comprise a unique identifier sequence to indicate origin of each library obtained by the reproducible complexity reduction; (b) combining the plurality of libraries to obtain a combined library and sequencing at least a portion of the combined library to obtain sequences; (c) aligning the sequences to obtain an alignment; and (d) identifying one or more polymorphisms in the plurality of nucleic acid samples.Type: GrantFiled: July 19, 2019Date of Patent: April 13, 2021Assignee: KEYGENE N.V.Inventors: Michael Josephus Theresia Van Eijk, Henricus Johannes Adam Van Der Poel
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Patent number: 10961545Abstract: The present invention relates to a new method for increasing drought resistance of a plant. The method encompasses the impairment of the expression of a gene or genes in said plant. In comparison to a plant not manipulated to impair the expression of said gene(s), the plants display improved drought resistance. Also provided are plants and plant product that can be obtained by the method according to the invention.Type: GrantFiled: April 30, 2019Date of Patent: March 30, 2021Assignee: KEYGENE N.V.Inventors: Anne Deslattes Mays, Marieke Helena Adriana Van Hulten, Shital Anilkumar Dixit, Martin De Vos, Jesse David Munkvold, Matthew Vitabile Dileo
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Publication number: 20210076584Abstract: It was found that plants with loss of functional Msi2 protein due to a nucleotide polymorphism resulting in the introduction of a premature stop codon in the Msi2 protein, are able to induce haploid offspring after a cross to or with a wild type plant comprising a functional Msi2 protein. The invention relates to generation of haploid and doubled haploid plants.Type: ApplicationFiled: November 30, 2020Publication date: March 18, 2021Applicant: Keygene N.V.Inventors: Rik Hubertus Martinus Op Den CAMP, Peter Johannes Van DIJK, Anthony GALLARD
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Publication number: 20210024951Abstract: The present invention relates to genetic elements comprising powdery mildew-conferring QTLs derived from a plant of the species Cucumis melo, or powdery mildew-conferring part or variant thereof. The invention also relates to markers for identification of said QTLs, use thereof and methods for producing plants with increased resistance to powdery mildew and the plants thus obtained.Type: ApplicationFiled: September 29, 2020Publication date: January 28, 2021Applicant: Keygene N.V.Inventors: Diana RIGOLA, René Johannes Maria HOFSTEDE, Magdalena Barbara LASTDRAGER, Jamila Maguy CHAÏB