Patents Assigned to Keygene, N. V.
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Patent number: 10023907Abstract: The present invention relates to a high throughput method for the identification and detection of molecular markers wherein restriction fragments are generated and suitable adaptors comprising (sample-specific) identifiers are ligated. The adapter-ligated restriction fragments may be selectively amplified with adaptor compatible primers carrying selective nucleotides at their 3? end. The amplified adapter-ligated restriction fragments are, at least partly, sequenced using high throughput sequencing methods and the sequence parts of the restriction fragments together with the sample-specific identifiers serve as molecular marker.Type: GrantFiled: May 22, 2014Date of Patent: July 17, 2018Assignee: Keygene N.V.Inventors: Michael Josephus Theresia Van Eijk, Rene Cornelis Josephus Hogers
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Publication number: 20180163222Abstract: The present invention relates to a new method for increasing drought resistance of a plant. The method encompasses the impairment of the expression of a gene or genes in said plant. In comparison to a plant not manipulated to impair the expression of said gene(s), the plants display improved drought resistance. Also provided are plants and plant products that can be obtained by the method according to the invention.Type: ApplicationFiled: January 26, 2018Publication date: June 14, 2018Applicant: Keygene N.V.Inventors: Anne DESLATTES MAYS, Marieke Helena Adriana VAN HULTEN, Shital Anilkumar DIXIT, Martin DE VOS, Jesse David MUNKVOLD, Matthew Vitabile DILEO
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Publication number: 20180137241Abstract: The invention relates to a method for identifying one or more polymorphisms in nucleic acid samples, comprising: (a) performing a reproducible complexity reduction on a plurality of nucleic acid samples to provide a plurality of libraries of the nucleic acid samples comprising amplified fragments, wherein the reproducible complexity reduction comprises amplifying fragments of the nucleic acid samples using one or more primers to obtain the amplified fragments, and wherein the amplified fragments in each library comprise a unique identifier sequence to indicate origin of each library obtained by the reproducible complexity reduction; (b) combining the plurality of libraries to obtain a combined library and sequencing at least a portion of the combined library to obtain sequences; (c) aligning the sequences to obtain an alignment; and (d) identifying one or more polymorphisms in the plurality of nucleic acid samples.Type: ApplicationFiled: January 5, 2018Publication date: May 17, 2018Applicant: Keygene N.V.Inventors: Michael Josephus Theresia VAN EIJK, Henricus Johannes Adam VAN DER POEL
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Patent number: 9909138Abstract: The present invention relates to a new method for increasing drought resistance of a plant. The method encompasses the impairment of the expression of a gene or genes in said plant. In comparison to a plant not manipulated to impair the expression of said gene(s), the plants display improved drought resistance. Also provided are plants and plant products that can be obtained by the method according to the invention.Type: GrantFiled: February 18, 2013Date of Patent: March 6, 2018Assignee: Keygene N.V.Inventors: Anne Deslattes Mays, Marieke Helena Adriana Van Hulten, Shital Anilkumar Dixit, Martin De Vos, Jesse David Munkvold, Matthew Vitabile Dileo
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Patent number: 9898576Abstract: The invention relates to a method for identifying one or more polymorphisms in nucleic acid samples, comprising: (a) performing a reproducible complexity reduction on a plurality of nucleic acid samples to provide a plurality of libraries of the nucleic acid samples comprising amplified fragments, wherein the reproducible complexity reduction comprises amplifying fragments of the nucleic acid samples using one or more primers to obtain the amplified fragments, and wherein the amplified fragments in each library comprise a unique identifier sequence to indicate origin of each library obtained by the reproducible complexity reduction; (b) combining the plurality of libraries to obtain a combined library and sequencing at least a portion of the combined library to obtain sequences; (c) aligning the sequences to obtain an alignment; and (d) identifying one or more polymorphisms in the plurality of nucleic acid samples.Type: GrantFiled: March 23, 2017Date of Patent: February 20, 2018Assignee: Keygene N.V.Inventors: Michael Josephus Theresia Van Eijk, Henricus Johannes Adam Van Der Poel
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Patent number: 9898577Abstract: The invention relates to a method for identifying one or more polymorphisms in nucleic acid samples, comprising: (a) performing a reproducible complexity reduction on a plurality of nucleic acid samples to provide a plurality of libraries of the nucleic acid samples comprising amplified fragments, wherein the reproducible complexity reduction comprises amplifying fragments of the nucleic acid samples using one or more primers to obtain the amplified fragments, and wherein the amplified fragments in each library comprise a unique identifier sequence to indicate origin of each library obtained by the reproducible complexity reduction; (b) combining the plurality of libraries to obtain a combined library and sequencing at least a portion of the combined library to obtain sequences; (c) aligning the sequences to obtain an alignment; and (d) identifying one or more polymorphisms in the plurality of nucleic acid samples.Type: GrantFiled: October 10, 2017Date of Patent: February 20, 2018Assignee: Keygene N.V.Inventors: Michael Josephus Theresia Van Eijk, Henricus Johannes Adam Van Der Poel
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Patent number: 9896721Abstract: The invention relates to a method for the high throughput identification of single nucleotide polymorphisms by performing a complexity reduction on two or more samples to yield two or more libraries, sequencing at least part of the libraries, aligning the identified sequences and determining any putative single nucleotide polymorphisms, confirming any putative single nucleotide polymorphism, generating detection probes for the confirmed single nucleotide polymorphisms, subjection a test sample to the same complexity reduction to provide a test library and screen the test library for the presence or absence of the single nucleotide polymorphisms using the detection probe.Type: GrantFiled: November 14, 2016Date of Patent: February 20, 2018Assignee: Keygene N.V.Inventors: Michael Josephus Theresia Van Eijk, Henricus Johannes Adam Van Der Poel
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Publication number: 20180023135Abstract: The invention relates to a method for the detection of a target nucleotide sequence in a sample based on an oligonucleotide ligation assay wherein probes are used that contain (a combination of) sequence-based identifiers that can identify the sample and the target sequence (i.e. locus and/or allele combination) wherein after the ligation step, the ligated probes, or after amplification, the amplified ligated probes, are restricted using restriction enzymes to cut of part of the probes and continue with those parts (identifiers and target sequence) that contain the relevant information in the sequencing step.Type: ApplicationFiled: August 22, 2017Publication date: January 25, 2018Applicant: Keygene N.V.Inventors: Michael Josephus Theresia VAN EIJK, René Cornelis Josephus HOGERS
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Publication number: 20180025111Abstract: The invention relates to a method for identifying one or more polymorphisms in nucleic acid samples, comprising: (a) performing a reproducible complexity reduction on a plurality of nucleic acid samples to provide a plurality of libraries of the nucleic acid samples comprising amplified fragments, wherein the reproducible complexity reduction comprises amplifying fragments of the nucleic acid samples using one or more primers to obtain the amplified fragments, and wherein the amplified fragments in each library comprise a unique identifier sequence to indicate origin of each library obtained by the reproducible complexity reduction; (b) combining the plurality of libraries to obtain a combined library and sequencing at least a portion of the combined library to obtain sequences; (c) aligning the sequences to obtain an alignment; and (d) identifying one or more polymorphisms in the plurality of nucleic acid samples.Type: ApplicationFiled: October 10, 2017Publication date: January 25, 2018Applicant: Keygene N.V.Inventors: Michael Josephus Theresia VAN EIJK, Henricus Johannes Adam VAN DER POEL
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Publication number: 20180016631Abstract: Disclosed is a method suitable for (long-range) mate pair sequencing wherein the mate pairs are located within a certain distance from each other on the same nucleotide sequence. By ligating a DNA fragment into an identifier section—containing backbone, a digestable circularized construct is provided to which adaptors can be ligated after digestion. Amplification yields amplicons that contain a combination of the identifier section with the terminal part of the fragments. The fragments are subsequently mated to each other to obtain a mated pair by identifying the corresponding identifier section in both amplicons. The mated pairs can be used in the construction of genome scaffolds or in the generation of draft genome sequences.Type: ApplicationFiled: December 23, 2015Publication date: January 18, 2018Applicant: Keygene N.V.Inventor: Michael Josephus Theresia VAN EIJK
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Publication number: 20180002751Abstract: The present invention relates to a method for identifying the source of an amplicon, comprising: providing a plurality of pools of amplicons from different sources, wherein the amplicons from different sources are present in more than one pool, and wherein the amplicons in each pool are tagged with a unique pool-specific identifier; sequencing at least part of the amplicons that comprise the pool-specific identifiers; and assigning one or more of the amplicons to corresponding pools and/or sources using the pool-specific identifiers.Type: ApplicationFiled: September 18, 2017Publication date: January 4, 2018Applicant: Keygene N.V.Inventors: Michael Josephus Theresia VAN EIJK, Taco Peter JESSE, Adrianus Johannes VAN TUNEN
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Patent number: 9856486Abstract: Trichome specific plant promoters are provided herein. Also provided are transgenic cells and organisms, especially plant cell and plants, comprising such trichome-specific promoter or a chimeric or vector comprising such trichome-specific promoter. The invention further provides methods for expressing nucleic acid sequences in cells and organisms using trichome specific promoters.Type: GrantFiled: November 29, 2013Date of Patent: January 2, 2018Assignee: Keygene N.V.Inventors: Paul Johan Diergaarde, Marinus Willem Prins, Martin De Vos
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Publication number: 20170356042Abstract: The invention relates to a method for the high throughput discovery, detection and genotyping of one or more genetic markers in one or more samples, comprising the steps of restriction endonuclease digest of DNA, adaptor-ligation, optional pre-amplification, selective amplification, pooling of the amplified products, sequencing the libraries with sufficient redundancy, clustering followed by identification of the genetic markers within the library and/or between libraries and determination of (co-)dominant genotypes of the genetic markers.Type: ApplicationFiled: August 22, 2017Publication date: December 14, 2017Applicant: Keygene N.V.Inventors: Michael Josephus Theresia VAN EIJK, Anker Preben SØRENSEN, Marco Gerardus Maria VAN SCHRIEK
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Patent number: 9834784Abstract: The disclosure provides an isolated nucleic acid molecule encoding a 7-epizingiberene synthase, a chimeric gene comprising said nucleic acid molecule, vectors comprising the same, as well as isolated 7-epizingiberene synthase proteins themselves. In addition, transgenic plants and plant cells comprising a gene encoding a 7-epizingiberene synthase, optionally integrated in its genome, and methods for making such plants and cells, are provided. Especially Solanaceae plants and plant parts (seeds, fruit, leaves, etc.) with enhanced insect pest resistance are provided.Type: GrantFiled: July 27, 2015Date of Patent: December 5, 2017Assignee: Keygene N.V.Inventors: Robert Cornelis Schuurink, Michael Albertus Haring, Petronella Martina Bleeker
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Publication number: 20170342486Abstract: Efficient methods are disclosed for the high throughput identification of mutations in genes in members of mutagenized populations. The methods comprise DNA isolation, pooling, amplification, creation of libraries, high throughput sequencing of libraries, preferably by sequencing-by-synthesis technologies, identification of mutations and identification of the member of the population carrying the mutation and identification of the mutation.Type: ApplicationFiled: August 10, 2017Publication date: November 30, 2017Applicant: Keygene N.V.Inventors: Michael Josephus Theresia VAN EIJK, Adrianus Johannes VAN TUNEN
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Publication number: 20170306347Abstract: The present invention provides use of a plant gene JAZ5a for improving drought-resistance of a plant. It further provides a method for improving the drought-resistance of a plant, comprising enhancing the expression or activity of Jaz5A in said plant.Type: ApplicationFiled: June 20, 2017Publication date: October 26, 2017Applicant: Keygene N.V.Inventor: Adrianus Johannes VAN TUNEN
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Patent number: 9777322Abstract: The invention relates to a method for the detection of a target nucleotide sequence in a sample based on an oligonucleotide ligation assay wherein probes are used that contain (a combination of) sequence-based identifiers that can identify the sample and the target sequence (i.e. locus and/or allele combination) wherein after the ligation step, the ligated probes, or after amplification, the amplified ligated probes, are restricted using restriction enzymes to cut of part of the probes and continue with those parts (identifiers and target sequence) that contain the relevant information in the sequencing step.Type: GrantFiled: July 9, 2012Date of Patent: October 3, 2017Assignee: Keygene N.V.Inventors: Michael Josephus Theresia Van Eijk, René Cornelis Josephus Hogers
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Patent number: 9777324Abstract: The invention relates to a method for the high throughput discovery, detection and genotyping of one or more genetic markers in one or more samples, comprising the steps of restriction endonuclease digest of DNA, adaptor-ligation, optional pre-amplification, selective amplification, pooling of the amplified products, sequencing the libraries with sufficient redundancy, clustering followed by identification of the genetic markers within the library and/or between libraries and determination of (co-)dominant genotypes of the genetic markers.Type: GrantFiled: April 22, 2016Date of Patent: October 3, 2017Assignee: Keygene N.V.Inventors: Michael Josephus Theresia Van Eijk, Anker Preben Sørensen, Marco Gerardus Maria Van Schriek
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Publication number: 20170247713Abstract: The present invention relates to identification and isolation of zinc finger transcription factor in tomato that specifically expresses in glandular trichomes of Solanum lycopersicum cultivar Moneymaker and binds to the promoters of the genes encoding Terpene Synthase 5 (also known as Monoterpene Synthase1) and Terpene Synthase 11 (also known as Sesquiterpene Synthase 1). The invention provides the isolated, recombinant or synthetic polynucleotides encoding the polypeptide sequences of SEQ ID NO:2 and variants and fragments thereof. The invention also provides constructs, vectors, host cells and plants genetically modified to contain the polynucleotides of the invention. The methods for producing plants with altered levels of terpenes, including transformed and mutant plants, are also provided.Type: ApplicationFiled: March 10, 2017Publication date: August 31, 2017Applicant: Keygene N.V.Inventors: Robert Cornelis SCHUURINK, Michael Albertus HARING, Eleni SPYROPOULOU
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Patent number: 9745627Abstract: Efficient methods are disclosed for the high throughput identification of mutations in genes in members of mutagenized populations. The methods comprise DNA isolation, pooling, amplification, creation of libraries, high throughput sequencing of libraries, preferably by sequencing-by-synthesis technologies, identification of mutations and identification of the member of the population carrying the mutation and identification of the mutation.Type: GrantFiled: February 16, 2017Date of Patent: August 29, 2017Assignee: Keygene N.V.Inventors: Michael Josephus Theresia van Eijk, Adrianus Johannes van Tunen