Patents Assigned to Keygene, N. V.
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Publication number: 20140274744Abstract: The invention relates to a method for the high throughput identification of single nucleotide polymorphisms by performing a complexity reduction on two or more samples to yield two or more libraries, sequencing at least part of the libraries, aligning the identified sequences and determining any putative single nucleotide polymorphisms, confirming any putative single nucleotide polymorphism, generating detection probes for the confirmed single nucleotide polymorphisms, subjection a test sample to the same complexity reduction to provide a test library and screen the test library for the presence or absence of the single nucleotide polymorphisms using the detection probe.Type: ApplicationFiled: April 15, 2014Publication date: September 18, 2014Applicant: Keygene N.V.Inventors: Michael Josephus Theresia Van EIJK, Henricus Johannes Adam Van Der Poel
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Patent number: 8815512Abstract: The invention relates to a method for the high throughput discovery, detection and genotyping of one or more genetic markers in one or more samples, comprising the steps of restriction endonuclease digest of DNA, adaptor-ligation, optional pre-amplification, selective amplification, pooling of the amplified products, sequencing the libraries with sufficient redundancy, clustering followed by identification of the genetic markers within the library and/or between libraries and determination of (co-)dominant genotypes of the genetic markers.Type: GrantFiled: November 1, 2012Date of Patent: August 26, 2014Assignee: KeyGene N.V.Inventors: Michael Josephus Theresia Van Eijk, Anker Preben Sørensen, Marco Geradus Maria Van Schriek
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Patent number: 8809626Abstract: Trichome specific plant promoters are provided herein. Also provided are transgenic cells and organisms, especially plant cell and plants, comprising an trichome specific promoter and methods for expressing nucleic acid sequences in cells and organisms using trichome specific promoters.Type: GrantFiled: December 16, 2008Date of Patent: August 19, 2014Assignee: KeyGene N.V.Inventors: Petronella Martina Bleeker, Christianus Cornelis Nicolaas Van Schie, Paul Johan Diergaarde, Robert Cornelis Schuurink, Michel Albertus Haring, Michiel Theodoor Jan De Both
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Patent number: 8808991Abstract: Method for the detection of a target sequence comprising ligating two probes when annealed adjacent to the target sequence, hybridization of a compound primer to the ligated probes and after elongation of the compound primer, amplifying the elongated compound primer from primers annealing to primer binding sites provided in the compound primer and one of the probes to produce detectably amplicons.Type: GrantFiled: August 31, 2004Date of Patent: August 19, 2014Assignee: Keygene N.V.Inventor: René Cornelis Josephus Hodgers
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Publication number: 20140221217Abstract: The invention relates to a method for the detection of a target nucleotide sequence in a sample based on an oligonucleotide ligation assay wherein probes are used that contain (a combination of) sequence-based identifiers that can identify the sample and the target sequence (i.e. locus and/or allele combination) wherein after the ligation step, the ligated probes, or after amplification, the amplified ligated probes, are restricted using restriction enzymes to cut of part of the probes and continue with those parts (identifiers and target sequence) that contain the relevant information in the sequencing step.Type: ApplicationFiled: July 9, 2012Publication date: August 7, 2014Applicant: Keygene N.V.Inventors: Michael Josephus Theresia Van Eijk, Rene Cornelis Josephus Hogers
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Publication number: 20140213462Abstract: The invention relates to a method for the high throughput identification of single nucleotide polymorphisms by performing a complexity reduction on two or more samples to yield two or more libraries, sequencing at least part of the libraries, aligning the identified sequences and determining any putative single nucleotide polymorphisms, confirming any putative single nucleotide polymorphism, generating detection probes for the confirmed single nucleotide polymorphisms, subjection a test sample to the same complexity reduction to provide a test library and screen the test library for the presence or absence of the single nucleotide polymorphisms using the detection probe.Type: ApplicationFiled: January 28, 2014Publication date: July 31, 2014Applicant: Keygene N.V.Inventors: Michael Josephus Theresia VAN EIJK, Henricus Johannes Adam Van Der Poel
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Publication number: 20140206551Abstract: The present invention relates to a high throughput method for the identification and detection of molecular markers wherein restriction fragments are generated and suitable adaptors comprising (sample-specific) identifiers are ligated. The adapter-ligated restriction fragments may be selectively amplified with adaptor compatible primers carrying selective nucleotides at their 3? end. The amplified adapter-ligated restriction fragments are, at least partly, sequenced using high throughput sequencing methods and the sequence parts of the restriction fragments together with the sample-specific identifiers serve as molecular markers.Type: ApplicationFiled: March 19, 2014Publication date: July 24, 2014Applicant: Keygene N.V.Inventors: Michael Josephus Theresia VAN EIJK, Taco Peter Jesse
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Publication number: 20140206850Abstract: The present invention relates to a new method for improving glyphosate resistance of a plant. The method encompasses providing one or more specific mutations in a specific nucleotide sequence in said plant. In comparison to a plant not manipulated according to the method, the plant obtained by the method displays (improved) glyphosate resistance. Also provided are a (transgenic) plant, including a seed thereof, and a plant product that can be obtained by the method according to the invention.Type: ApplicationFiled: April 27, 2012Publication date: July 24, 2014Applicant: KEYGENE N.V.Inventor: Paul Bundock
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Patent number: 8785353Abstract: The invention relates to a method for the high throughput identification of single nucleotide polymorphisms by performing a complexity reduction on two or more samples to yield two or more libraries, sequencing at least part of the libraries, aligning the identified sequences and determining any putative single nucleotide polymorphisms, confirming any putative single nucleotide polymorphism, generating detection probes for the confirmed single nucleotide polymorphisms, subjection a test sample to the same complexity reduction to provide a test library and screen the test library for the presence or absence of the single nucleotide polymorphisms using the detection probe.Type: GrantFiled: June 23, 2006Date of Patent: July 22, 2014Assignee: Keygene N.V.Inventors: Michael Josephus Theresia Van Eijk, Henricus Johannes Adam Van Der Poel
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Publication number: 20140189908Abstract: The present invention relates to powdery mildew resistance providing genes of the Cucumis family, and especially Cucumis melo, wherein said resistance is provided by impairment of the present genes. Further, the present invention relates plants comprising the present impaired resistance conferring genes and seeds, embryos or other propagation material thereof. Especially, the present invention relates to powdery mildew resistance conferring genes, wherein the amino acid sequence encoded by said resistance conferring gene is selected from the group consisting of SEQ ID No. 2, SEQ ID No. 4, SEQ ID No. 6, SEQ ID No. 8, SEQ ID No. 10, SEQ ID No. 12 and SEQ ID No. 14, and amino acid sequences with more than 70% identity, preferably more than 80% identity, more preferably more than 90% identity, and most preferably more than 95% identity.Type: ApplicationFiled: February 24, 2012Publication date: July 3, 2014Applicants: KEYGENE N.V., ENZA ZADEN BEHEER B.V.Inventors: Paul Johan Diergaarde, Leonora Johanna Gertruda Van Enckevort, Karin Ingeborg Posthuma, Marinus Willem Prins
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Publication number: 20140157454Abstract: The present invention relates to powdery mildew resistance providing genes of the Cucumis family, and especially Cucumis sativus, wherein said resistance is provided by impairment of the present genes. Further, the present invention relates plants comprising the present impaired resistance conferring genes and seeds, embryos or other propagation material thereof. Especially, the present invention relates to powdery mildew resistance conferring genes, wherein the amino acid sequence encoded by said resistance conferring gene is selected from the group consisting of SEQ ID No. 2, SEQ ID No. 4, SEQ ID No. 6, SEQ ID No. 8, SEQ ID No. 10, SEQ ID No. 12, SEQ ID No. 14, SEQ ID No. 16, SEQ ID No. 18, SEQ ID No. 20 and SEQ ID No. 22, and amino acid sequences with more than 70% identity, preferably more than 80% identity, more preferably more than 90% identity, and most preferably more than 95% identity.Type: ApplicationFiled: February 20, 2012Publication date: June 5, 2014Applicants: KEYGENE N.V., ENZA ZADEN BEHEER B.V.Inventors: Paul Johan Diergaarde, Leonora Johanna Gertruda Van Enckevort, Karin Ingeborg Posthuma, Marinus Willem Prins
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Patent number: 8716550Abstract: The present invention relates to a method for the provision of mutagenised populations by the introduction of mutations by chemically and or physically mutagens in a selected region of the genome of a plant and the subsequent selection of inheritable mutations by analysis of at least one section of a mutagenised primary M1 plant for the presence of a mutation in the region of interest using high throughput sequencing.Type: GrantFiled: September 24, 2008Date of Patent: May 6, 2014Assignee: Keygene N.V.Inventor: Jeroen Stuurman
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Patent number: 8685650Abstract: The present invention relates to a high throughput method for the identification and detection of molecular markers wherein restriction fragments are generated and suitable adaptors comprising (sample-specific) identifiers are ligated. The adapter-ligated restriction fragments may be selectively amplified with adaptor compatible primers carrying selective nucleotides at their 3? end. The amplified adapter-ligated restriction fragments are, at least partly, sequenced using high throughput sequencing methods and the sequence parts of the restriction fragments together with the sample-specific identifiers serve as molecular markers.Type: GrantFiled: March 4, 2013Date of Patent: April 1, 2014Assignee: Keygene N.V.Inventors: Michael Josephus Theresia Van Eijk, Taco Peter Jesse
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Patent number: 8685889Abstract: The invention relates to a method for the high throughput identification of single nucleotide polymorphisms by performing a complexity reduction on two or more samples to yield two or more libraries, sequencing at least part of the libraries, aligning the identified sequences and determining any putative single nucleotide polymorphisms, confirming any putative single nucleotide polymorphism, generating detection probes for the confirmed single nucleotide polymorphisms, subjection a test sample to the same complexity reduction to provide a test library and screen the test library for the presence or absence of the single nucleotide polymorphisms using the detection probe.Type: GrantFiled: April 18, 2012Date of Patent: April 1, 2014Assignee: Keygene N.V.Inventors: Michael Josephus Theresia van Eijk, Henricus Johannes Adam van der Poel
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Publication number: 20140080716Abstract: Efficient methods are disclosed for the high throughput identification of mutations in genes in members of mutagenized populations. The methods comprise DNA isolation, pooling, amplification, creation of libraries, high throughput sequencing of libraries, preferably by sequencing-by-synthesis technologies, identification of mutations and identification of the member of the population carrying the mutation and identification of the mutation.Type: ApplicationFiled: August 21, 2013Publication date: March 20, 2014Applicant: Keygene N.V.Inventors: Michael Josephus Theresia VAN EIJK, Adrianus Johannes van Tunen
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Patent number: 8674175Abstract: A new farnesene synthase was isolated from tomato. The farnesene synthase shows surprising properties with regard to the end products formed and its gene has, on a nucleotide level, low sequence identity with known farnesene synthase genes from other sources. The invention relates to isolated polynucleotides, polypeptides encoded by said polynucleotides, genetic constructs, vectors, hosts, in particular plants, harboring such polynucleotides, polypeptides and genetic constructs, and seed derived from such plants.Type: GrantFiled: December 2, 2009Date of Patent: March 18, 2014Assignee: Keygene N.V.Inventors: Michiel Theodoor Jan de Both, Robert Cornelis Schuurink, Kai Ament, Michael Albertus Haring
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Publication number: 20140004607Abstract: The current invention relates to a method for targeted alteration of acceptor DNA, for example duplex acceptor DNA. The method comprises the use of an oligonucleotide having at least one mismatch relative to the targeted (duplex) acceptor DNA. The mismatch is located at specific positions within said oligonucleotide. Also provided is a kit that comprises instructions for performing the method according to the inventions, and in a preferred embodiment, comprises an oligonucleotide suitable for use in the method.Type: ApplicationFiled: November 25, 2011Publication date: January 2, 2014Applicant: KEYGENE N.V.Inventors: Paul Bundock, Franck Lhuissier
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Patent number: 8614073Abstract: Efficient methods are disclosed for the high throughput identification of mutations in genes in members of mutagenized populations. The methods comprise DNA isolation, pooling, amplification, creation of libraries, high throughput sequencing of libraries, preferably by sequencing-by-synthesis technologies, identification of mutations and identification of the member of the population carrying the mutation and identification of the mutation.Type: GrantFiled: April 16, 2012Date of Patent: December 24, 2013Assignee: Keygene N.V.Inventors: Michael Josephus Theresia van Eijk, Adrianus Johannes van Tunen
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Publication number: 20130184166Abstract: The present invention relates to a high throughput method for the identification and detection of molecular markers wherein restriction fragments are generated and suitable adaptors comprising (sample-specific) identifiers are ligated. The adapter-ligated restriction fragments may be selectively amplified with adaptor compatible primers carrying selective nucleotides at their 3? end. The amplified adapter-ligated restriction fragments are, at least partly, sequenced using high throughput sequencing methods and the sequence parts of the restriction fragments together with the sample-specific identifiers serve as molecular markers.Type: ApplicationFiled: March 4, 2013Publication date: July 18, 2013Applicant: Keygene N.V.Inventor: Keygene N.V.
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Patent number: 8481257Abstract: The invention relates to a method for the high throughput discovery, detection and genotyping of one or more genetic markers in one or more samples, comprising the steps of restriction endonuclease digest of DNA, adaptor-ligation, optional pre-amplification, selective amplification, pooling of the amplified products, sequencing the libraries with sufficient redundancy, clustering followed by identification of the genetic markers within the library and/or between libraries and determination of (co-) dominant genotypes of the genetic markers.Type: GrantFiled: December 20, 2006Date of Patent: July 9, 2013Assignee: Keygene N.V.Inventors: Michael Josephus Theresia Van Eijk, Anker Preben Sørensen, Marco Gerardus Maria Van Schriek