Patents Assigned to Keygene, N. V.
-
Publication number: 20160010108Abstract: The present invention relates to a new method for increasing drought resistance of a plant. The method encompasses the impairment of the expression of a gene or genes in said plant. In comparison to a plant not manipulated to impair the expression of said gene(s), the plants display improved drought resistance. Also provided are plants and plant products that can be obtained by the method according to the invention.Type: ApplicationFiled: February 18, 2013Publication date: January 14, 2016Applicant: KEYGENE N.V.Inventors: Anne DESLATTES MAYS, Marieke Helena Adriana VAN HULTEN, Shital Anilkumar DIXIT, Martin DE VOS, Jesse David MUNKVOLD, Matthew Vitabile DILEO
-
Publication number: 20160010107Abstract: The present invention relates to a new method for increasing drought resistance of a plant. The method encompasses the impairment of the expression of a gene or genes in said plant. In comparison to a plant not manipulated to impair the expression of said gene(s), the plants display improved drought resistance. Also provided are plants and plant product that can be obtained by the method according to the invention.Type: ApplicationFiled: February 18, 2013Publication date: January 14, 2016Applicant: KEYGENE N.V.Inventors: Anne DESLATTES MAYS, Marieke Helena Adriana VAN HULTEN, Shital Anilkumar DIXIT, Martin DE VOS, Jesse David MUNKVOLD, Matthew Vitabile DILEO, Evert-Jan BLOM
-
Patent number: 9234193Abstract: An isolated nucleic acid molecule encoding a 7-epizingiberene synthase, a chimeric gene comprising the nucleic acid molecule, vectors comprising the same, as well as isolated 7-epizingiberene synthase proteins themselves, are provided. In addition, transgenic plants and plant cells comprising a gene encoding a 7-epizingiberene synthase, optionally integrated in its genome, and methods for making such plants and cells, are provided. Especially Solanaceae plants and plant parts (seeds, fruit, leaves, etc.) with enhanced insect pest resistance are provided.Type: GrantFiled: May 31, 2012Date of Patent: January 12, 2016Assignee: Keygene N.V.Inventors: Robert Cornelis Schuurink, Michael Albertus Haring, Petronella Martina Bleeker
-
Publication number: 20150351340Abstract: The current disclosure relates to the field of plants, in particular to the fields of plant breeding and plant genetics. More particular, the disclosure concerns inventive methodology that may be useful in improving plant properties. In particular the invention may be useful in removing linkage drag. Also provided are plant and plant parts obtained with the method disclosed herein.Type: ApplicationFiled: December 20, 2013Publication date: December 10, 2015Applicant: KEYGENE N.V.Inventors: Paul BUNDOCK, Jeroen STUURMAN
-
Publication number: 20150344946Abstract: The invention relates to a method for the high throughput discovery, detection and genotyping of one or more genetic markers in one or more samples, comprising the steps of restriction endonuclease digest of DNA, adaptor-ligation, optional pre-amplification, selective amplification, pooling of the amplified products, sequencing the libraries with sufficient redundancy, clustering followed by identification of the genetic markers within the library and/or between libraries and determination of (co-)dominant genotypes of the genetic markers.Type: ApplicationFiled: April 29, 2015Publication date: December 3, 2015Applicant: Keygene N.V.Inventors: Michael Josephus Theresia VAN EIJK, Anker Preben Sørensen, Marco Gerardus Maria Van Schriek
-
Publication number: 20150329906Abstract: The invention relates to a method for the determination of a genome sequence comprising the steps of providing a physical map of a sample genome by sequencing fragment ends of pooled BAC clones; providing a set of sequence reads from a sample genome generating a contig of the physical map and the sequence reads.Type: ApplicationFiled: July 24, 2015Publication date: November 19, 2015Applicant: Keygene N.V.Inventors: Michael Josephus Theresia VAN EIJK, Adrianus Johannes VAN TUNEN, Antoine Antonius Arnoldus Wilhelmus JANSSEN, Taco Peter JESSE
-
Publication number: 20150315602Abstract: Trichome specific plant promoters are provided herein. Also provided are transgenic cells and organisms, especially plant cell and plants, comprising such trichome-specific promoter or a chimeric or vector comprising such trichome-specific promoter. The invention further provides methods for expressing nucleic acid sequences in cells and organisms using trichome specific promoters.Type: ApplicationFiled: November 29, 2013Publication date: November 5, 2015Applicant: Keygene N.V.Inventors: Paul Johan DIERGAARDE, Marinus Willem PRINS, Martin DE VOS
-
Publication number: 20150284789Abstract: The method of the present invention now provides a technique for generating sequence information from nucleic acid samples based on knowledge from part(s) of the nucleotide sequence. The knowledge of the partial sequence may include knowledge about the presence of restriction sites. The knowledge of the partial sequence can be used to generate adaptor ligated or nucleotide-elongated fragments. From the combination of information on the ligated adaptor and the Known Nucleotide Sequence Section, probes can be designed. The probes can be used in the provision of circularised fragments that can be sequenced. Combining the known and determined sequences adds sequence information to the already existing sequence information and complements the available genomic sequence information.Type: ApplicationFiled: June 17, 2015Publication date: October 8, 2015Applicant: Keygene N.V.Inventor: René Cornelis Josephus HOGERS
-
Patent number: 9150854Abstract: The current invention relates to a method for targeted alteration of acceptor DNA, for example duplex acceptor DNA. The method comprises the use of an oligonucleotide having at least one mismatch relative to the targeted (duplex) acceptor DNA. The mismatch is located at specific positions within said oligonucleotide. Also provided is a kit that comprises instructions for performing the method according to the inventions, and in a preferred embodiment, comprises an oligonucleotide suitable for use in the method.Type: GrantFiled: November 25, 2011Date of Patent: October 6, 2015Assignee: Keygene N.V.Inventors: Paul Bundock, Franck Lhuissier
-
Publication number: 20150232924Abstract: The invention relates to a method for the high throughput identification of single nucleotide polymorphisms by performing a complexity reduction on two or more samples to yield two or more libraries, sequencing at least part of the libraries, aligning the identified sequences and determining any putative single nucleotide polymorphisms, confirming any putative single nucleotide polymorphism, generating detection probes for the confirmed single nucleotide polymorphisms, subjection a test sample to the same complexity reduction to provide a test library and screen the test library for the presence or absence of the single nucleotide polymorphisms using the detection probe.Type: ApplicationFiled: February 19, 2015Publication date: August 20, 2015Applicant: Keygene N.V.Inventors: Michael Josephus Theresia VAN EIJK, Henricus Johannes Adam Van der Poel
-
Patent number: 9090888Abstract: The disclosure provides an isolated nucleic acid molecule encoding a 7-epizingiberene synthase, a chimeric gene comprising said nucleic acid molecule, vectors comprising the same, as well as isolated 7-epizingiberene synthase proteins themselves. In addition, transgenic plants and plant cells comprising a gene encoding a 7-epizingiberene synthase, optionally integrated in its genome, and methods for making such plants and cells, are provided. Especially Solanaceae plants and plant parts (seeds, fruit, leaves, etc.) with enhanced insect pest resistance are provided.Type: GrantFiled: December 15, 2014Date of Patent: July 28, 2015Assignee: Keygene N.V.Inventors: Robert Cornelis Schuurink, Michael Albertus Haring, Petronella Martina Bleeker
-
Patent number: 9080210Abstract: The invention involves methods and uses of a combination of at least two nucleotide sequence identifiers in the preparation of a sample DNA for high throughput sequencing. Accordingly, in the high throughput sequencing a plurality of prepared sample DNAs, each preparation of a sample DNA comprises a unique combination of the at least two nucleotide sequence identifiers wherein a first nucleotide sequence identifier is selected from a group of nucleotide sequence identifiers and a second nucleotide sequence identifier is selected from the group of nucleotide sequence identifiers.Type: GrantFiled: June 8, 2011Date of Patent: July 14, 2015Assignee: Keygene N.V.Inventors: Michael Josephus Theresia Van Eijk, Henricus Johannes Adam Van Der Poel
-
Patent number: 9062348Abstract: The invention relates to a method for the high throughput discovery, detection and genotyping of one or more genetic markers in one or more samples, comprising the steps of restriction endonuclease digest of DNA, adaptor-ligation, optional pre-amplification, selective amplification, pooling of the amplified products, sequencing the libraries with sufficient redundancy, clustering followed by identification of the genetic markers within the library and/or between libraries and determination of (co-)dominant genotypes of the genetic markers.Type: GrantFiled: November 21, 2014Date of Patent: June 23, 2015Assignee: Keygene N.V.Inventors: Michael Josephus Theresia Van Eijk, Anker Preben Sørensen, Marco Gerardus Maria Van Schriek
-
Publication number: 20150159217Abstract: The invention relates to a method for the high throughput discovery, detection and genotyping of one or more genetic markers in one or more samples, comprising the steps of restriction endonuclease digest of DNA, adaptor-ligation, optional pre-amplification, selective amplification, pooling of the amplified products, sequencing the libraries with sufficient redundancy, clustering followed by identification of the genetic markers within the library and/or between libraries and determination of (co-)dominant genotypes of the genetic markers.Type: ApplicationFiled: November 21, 2014Publication date: June 11, 2015Applicant: Keygene N.V.Inventors: Michael Josephus Theresia VAN EIJK, Anker Preben Sørensen, Marco Gerardus Maria Van Schriek
-
Publication number: 20150148241Abstract: The present invention relates to a high throughput method for the identification and detection of molecular markers wherein restriction fragments are generated and suitable adaptors comprising (sample-specific) identifiers are ligated. The adapter-ligated restriction fragments may be selectively amplified with adaptor compatible primers carrying selective nucleotides at their 3? end. The amplified adapter-ligated restriction fragments are, at least partly, sequenced using high throughput sequencing methods and the sequence parts of the restriction fragments together with the sample-specific identifiers serve as molecular markers.Type: ApplicationFiled: February 4, 2015Publication date: May 28, 2015Applicant: Keygene N.V.Inventors: Michael Josephus Theresia VAN EIJK, Taco Peter JESSE
-
Patent number: 9023768Abstract: The invention relates to a method for the high throughput identification of single nucleotide polymorphisms by performing a complexity reduction on two or more samples to yield two or more libraries, sequencing at least part of the libraries, aligning the identified sequences and determining any putative single nucleotide polymorphisms, confirming any putative single nucleotide polymorphism, generating detection probes for the confirmed single nucleotide polymorphisms, subjection a test sample to the same complexity reduction to provide a test library and screen the test library for the presence or absence of the single nucleotide polymorphisms using the detection probe.Type: GrantFiled: April 15, 2014Date of Patent: May 5, 2015Assignee: Keygene N.V.Inventors: Michael Josephus Theresia Van Eijk, Henricus Johannes Adam Van Der Poel
-
Publication number: 20150101084Abstract: The disclosure provides an isolated nucleic acid molecule encoding a 7-epizingiberene synthase, a chimeric gene comprising said nucleic acid molecule, vectors comprising the same, as well as isolated 7-epizingiberene synthase proteins themselves. In addition, transgenic plants and plant cells comprising a gene encoding a 7-epizingiberene synthase, optionally integrated in its genome, and methods for making such plants and cells, are provided. Especially Solanaceae plants and plant parts (seeds, fruit, leaves, etc.) with enhanced insect pest resistance are provided.Type: ApplicationFiled: December 15, 2014Publication date: April 9, 2015Applicant: Keygene N.V.Inventors: Robert Cornelis SCHUURINK, Michael Albertus Haring, Petronella Martina Bleeker
-
Patent number: 8975028Abstract: The present invention relates to a high throughput method for the identification and detection of molecular markers wherein restriction fragments are generated and suitable adaptors comprising (sample-specific) identifiers are ligated. The adapter-ligated restriction fragments may be selectively amplified with adaptor compatible primers carrying selective nucleotides at their 3? end. The amplified adapter-ligated restriction fragments are, at least partly, sequenced using high throughput sequencing methods and the sequence parts of the restriction fragments together with the sample-specific identifiers serve as molecular markers.Type: GrantFiled: March 19, 2014Date of Patent: March 10, 2015Assignee: Keygene N.V.Inventors: Michael Josephus Theresia Van Eijk, Taco Peter Jesse
-
Patent number: 8932812Abstract: Method for de novo whole genome sequencing based on a (sequence-based) physical map of a DNA sample clone bank based on end-sequencing tagged adapter-ligated restriction fragments, in combination with sequencing adapter-ligated restriction fragments of the DNA sample wherein the recognition sequence of the restriction enzyme used in the generation of the physical map is identical to at least part of the recognition sequence of the restriction enzyme used in the generation of the DNA sample.Type: GrantFiled: December 16, 2010Date of Patent: January 13, 2015Assignee: KeyGene N.V.Inventors: René Cornelis Josephus Hogers, Michael Josephus Theresia van Eijk
-
Patent number: 8911945Abstract: The invention relates to a method for the high throughput discovery, detection and genotyping of one or more genetic markers in one or more samples, comprising the steps of restriction endonuclease digest of DNA, adaptor-ligation, optional pre-amplification, selective amplification, pooling of the amplified products, sequencing the libraries with sufficient redundancy, clustering followed by identification of the genetic markers within the library and/or between libraries and determination of (co-)dominant genotypes of the genetic markers.Type: GrantFiled: June 27, 2014Date of Patent: December 16, 2014Assignee: KeyGene N.V.Inventors: Michael Josephus Theresia Van Eijk, Anker Preben Sørensen, Marco Gerardus Maria Van Schriek