Patents Assigned to Keygene, N. V.
  • Strategies for high throughput identification and detection of polymorphisms
    Patent number: 9447459
    Abstract: The invention relates to a method for the high throughput identification of single nucleotide polymorphisms by performing a complexity reduction on two or more samples to yield two or more libraries, sequencing at least part of the libraries, aligning the identified sequences and determining any putative single nucleotide polymorphisms, confirming any putative single nucleotide polymorphism, generating detection probes for the confirmed single nucleotide polymorphisms, subjection a test sample to the same complexity reduction to provide a test library and screen the test library for the presence or absence of the single nucleotide polymorphisms using the detection probe.
    Type: Grant
    Filed: January 28, 2014
    Date of Patent: September 20, 2016
    Assignee: Keygene N.V.
    Inventors: Michael Josephus Theresia Van Eijk, Henricus Johannes Adam Van Der Poel
  • PLANT VOLATILES
    Publication number: 20160255833
    Abstract: The present invention provides compounds and compositions for attracting or repelling sap-sucking insects, such as whitefly, as well as methods for using such compositions.
    Type: Application
    Filed: May 16, 2016
    Publication date: September 8, 2016
    Applicant: Keygene N.V.
    Inventors: Petronella Martina BLEEKER, Kai AMENT, Paul Johan DIERGAARDE, Michiel Theodoor Jan DE BOTH, Robert Cornelis SCHUURINK
  • METHOD FOR HIGH-THROUGHPUT AFLP-BASED POLYMORPHISM DETECTION
    Publication number: 20160258013
    Abstract: The invention relates to a method for the high throughput discovery, detection and genotyping of one or more genetic markers in one or more samples, comprising the steps of restriction endonuclease digest of DNA, adaptor-ligation, optional pre-amplification, selective amplification, pooling of the amplified products, sequencing the libraries with sufficient redundancy, clustering followed by identification of the genetic markers within the library and/or between libraries and determination of (co-)dominant genotypes of the genetic markers.
    Type: Application
    Filed: April 22, 2016
    Publication date: September 8, 2016
    Applicant: Keygene N.V.
    Inventors: Michael Josephus Theresia VAN EIJK, Anker Preben SØRENSEN, Marco Gerardus Maria VAN SCHRIEK
  • HIGH THROUGHPUT SCREENING OF MUTAGENIZED POPULATIONS
    Publication number: 20160258006
    Abstract: Efficient methods are disclosed for the high throughput identification of mutations in genes in members of mutagenized populations. The methods comprise DNA isolation, pooling, amplification, creation of libraries, high throughput sequencing of libraries, preferably by sequencing-by-synthesis technologies, identification of mutations and identification of the member of the population carrying the mutation and identification of the mutation.
    Type: Application
    Filed: May 26, 2016
    Publication date: September 8, 2016
    Applicant: Keygene N.V.
    Inventors: Michael Josephus Theresia van EIJK, Adrianus Johannes van TUNEN
  • METHOD FOR GENOME SEQUENCING USING A SEQUENCE-BASED PHYSICAL MAP
    Publication number: 20160251713
    Abstract: The present invention relates to a high throughput method for the identification and detection of molecular markers wherein restriction fragments are generated and suitable adaptors comprising (sample-specific) identifiers are ligated. The adapter-ligated restriction fragments may be selectively amplified with adaptor compatible primers carrying selective nucleotides at their 3? end. The amplified adapter-ligated restriction fragments are, at least partly, sequenced using high throughput sequencing methods and the sequence parts of the restriction fragments together with the sample-specific identifiers serve as molecular markers.
    Type: Application
    Filed: February 3, 2016
    Publication date: September 1, 2016
    Applicant: Keygene N.V.
    Inventors: Michael Josephus Theresia VAN EIJK, Taco Peter JESSE
  • PLANT VOLATILES BASED ON R-CURCUMENE
    Publication number: 20160212991
    Abstract: The invention provides a method for controlling whiteflies which comprises the steps of: providing a composition comprising or consisting of R-curcumene and optionally further beta-myrcene, para-cymene, gamma-terpinene, alpha-terpinene, alpha-phellandrene zingiberene and/or 7-epi-zingiberene; and adding said composition one or more times to a plurality of crop plants. The method can be combined with the use of attractant compositions comprising beta-phellandrene, limonene and/or 2-carene, and optionally adding said composition one or more times to one or more trap plants and/or trap materials.
    Type: Application
    Filed: April 5, 2016
    Publication date: July 28, 2016
    Applicant: Keygene N.V.
    Inventors: Petronella Martina BLEEKER, Kai AMENT, Paul Johan DIERGAARDE, Michiel Theodoor Jan de BOTH, Robert Cornelis SCHUURINK
  • High throughput screening of mutagenized populations
    Patent number: 9376719
    Abstract: Efficient methods are disclosed for the high throughput identification of mutations in genes in members of mutagenized populations. The methods comprise DNA isolation, pooling, amplification, creation of libraries, high throughput sequencing of libraries, preferably by sequencing-by-synthesis technologies, identification of mutations and identification of the member of the population carrying the mutation and identification of the mutation.
    Type: Grant
    Filed: August 21, 2013
    Date of Patent: June 28, 2016
    Assignee: Keygene N.V.
    Inventors: Michael Josephus Theresia van Eijk, Adrianus Johannes van Tunen
  • Strategies for high throughput identification and detection of polymorphisms
    Patent number: 9376716
    Abstract: The invention relates to a method for the high throughput identification of single nucleotide polymorphisms by performing a complexity reduction on two or more samples to yield two or more libraries, sequencing at least part of the libraries, aligning the identified sequences and determining any putative single nucleotide polymorphisms, confirming any putative single nucleotide polymorphism, generating detection probes for the confirmed single nucleotide polymorphisms, subjection a test sample to the same complexity reduction to provide a test library and screen the test library for the presence or absence of the single nucleotide polymorphisms using the detection probe.
    Type: Grant
    Filed: October 16, 2015
    Date of Patent: June 28, 2016
    Assignee: Keygene N.V.
    Inventors: Michael Josephus Theresia Van Eijk, Henricus Johannes Adam Van Der Poel
  • Mutagenesis method using polyethylene glycol mediated introduction of mutagenic nucleobases into plant protoplasts
    Patent number: 9365860
    Abstract: Method for targeted alteration of a duplex acceptor DNA sequence in a plant cell protoplast, comprising combining the duplex acceptor DNA sequence with a donor mutagenic nucleobase, wherein the duplex acceptor DNA sequence contains a first DNA sequence and a second DNA sequence which is the complement of the first DNA sequence and wherein the donor mutagenic nucleobase comprises at least one mismatch with respect to the duplex acceptor DNA sequence to be altered, preferably with respect to the first DNA sequence, wherein the method further comprises a step of introducing the donor mutagenic nucleobase into the cell protoplasts using polyethylene glycol (PEG) mediated transformation and the use of PEG protoplast transformation for enhancing the rate of targeted mutagenesis.
    Type: Grant
    Filed: July 17, 2012
    Date of Patent: June 14, 2016
    Assignee: KEYGENE N.V.
    Inventors: Paul Bundock, Michiel Theodoor Jan De Both, Franck Lhuissier
  • Plant volatiles
    Patent number: 9364002
    Abstract: The present invention provides compounds and compositions for attracting or repelling sap-sucking insects, such as whitefly, as well as methods for using such compositions.
    Type: Grant
    Filed: September 25, 2008
    Date of Patent: June 14, 2016
    Assignee: Keygene N.V.
    Inventors: Petronella Martina Bleeker, Kai Ament, Paul Johan Diergaarde, Michiel Theodoor Jan De Both, Robert Cornelis Schuurink
  • STRATEGIES FOR HIGH THROUGHPUT IDENTIFICATION AND DETECTION OF POLYMORPHISMS
    Publication number: 20160145689
    Abstract: The invention relates to a method for the high throughput identification of single nucleotide polymorphisms by performing a complexity reduction on two or more samples to yield two or more libraries, sequencing at least part of the libraries, aligning the identified sequences and determining any putative single nucleotide polymorphisms, confirming any putative single nucleotide polymorphism, generating detection probes for the confirmed single nucleotide polymorphisms, subjection a test sample to the same complexity reduction to provide a test library and screen the test library for the presence or absence of the single nucleotide polymorphisms using the detection probe.
    Type: Application
    Filed: January 28, 2016
    Publication date: May 26, 2016
    Applicant: Keygene N.V.
    Inventors: Michael Josephus Theresia VAN EIJK, Henricus Johannes Adam Van der POEL
  • DIRECTED STRATEGIES FOR IMPROVING PHENOTYPIC TRAITS
    Publication number: 20160132635
    Abstract: The present invention provides a method for improving at least one phenotypic trait of interest in subsequent generation(s) of a population of individuals, preferably crop plants or cattle. Particularly, the method identifies the combination of at least three individuals that gives, upon subsequent intercrossing, the highest estimated probability of improving the at least one phenotypic trait of interest in the subsequent generation(s). Also provided is a computer-readable medium comprising instructions for performing the method.
    Type: Application
    Filed: June 13, 2014
    Publication date: May 12, 2016
    Applicant: Keygene N.V.
    Inventors: Jacob Bernhard BUNTJER, José Lúcio Lima GUERRA, Timotheus Gerardus DOESWIJK, Remco VAN BERLOO, Niek BOUMAN
  • Method for high-throughput AFLP-based polymorphism detection
    Patent number: 9334536
    Abstract: The invention relates to a method for the high throughput discovery, detection and genotyping of one or more genetic markers in one or more samples, comprising the steps of restriction endonuclease digest of DNA, adaptor-ligation, optional pre-amplification, selective amplification, pooling of the amplified products, sequencing the libraries with sufficient redundancy, clustering followed by identification of the genetic markers within the library and/or between libraries and determination of (co-)dominant genotypes of the genetic markers.
    Type: Grant
    Filed: May 9, 2014
    Date of Patent: May 10, 2016
    Assignee: Keygene N.V.
    Inventors: Michael Josephus Theresia Van Eijk, Anker Preben Sørensen, Marco Gerardus Maria Van Schriek
  • Plant volatiles based on R-curcumene
    Patent number: 9332753
    Abstract: The invention provides a method for controlling whiteflies which comprises the steps of: providing a composition comprising or consisting of R-curcumene and optionally further beta-myrcene, para-cymene, gamma-terpinene, alpha-terpinene, alpha-phellandrene zingiberene and/or 7-epi-zingiberene; and adding said composition one or more times to a plurality of crop plants. The method can be combined with the use of attractant compositions comprising beta-phellandrene, limonene and/or 2-carene, and optionally adding said composition one or more times to one or more trap plants and/or trap materials.
    Type: Grant
    Filed: March 4, 2010
    Date of Patent: May 10, 2016
    Assignee: Keygene N.V.
    Inventors: Petronella Martina Bleeker, Kai Ament, Paul Johan Diergaarde, Michiel Theodoor Jan de Both, Robert Cornelis Schuurink
  • Method for high-throughput AFLP-based polymorphism detection
    Patent number: 9328383
    Abstract: The invention relates to a method for the high throughput discovery, detection and genotyping of one or more genetic markers in one or more samples, comprising the steps of restriction endonuclease digest of DNA, adaptor-ligation, optional pre-amplification, selective amplification, pooling of the amplified products, sequencing the libraries with sufficient redundancy, clustering followed by identification of the genetic markers within the library and/or between libraries and determination of (co-)dominant genotypes of the genetic markers.
    Type: Grant
    Filed: April 29, 2015
    Date of Patent: May 3, 2016
    Assignee: Keygene N.V.
    Inventors: Michael Josephus Theresia Van Eijk, Anker Preben Sørensen, Marco Gerardus Maria Van Schriek
  • METHOD FOR TARGETED SEQUENCING
    Publication number: 20160083788
    Abstract: The method of the present invention now provides a technique for generating sequence information from nucleic acid samples based on knowledge from part(s) of the nucleotide sequence. The knowledge of the partial sequence may include knowledge about the presence of restriction sites. The knowledge of the partial sequence can be used to generate adaptor-ligated or nucleotide-elongated fragments. From the combination of information on the ligated adaptor and the Known Nucleotide Sequence Section, probes can be designed. The probes can be used in the provision of circularized fragments that can be sequenced. Combining the known and determined sequences adds sequence information to the already existing sequence information and complements the available genomic sequence information.
    Type: Application
    Filed: June 6, 2014
    Publication date: March 24, 2016
    Applicant: Keygene N.V.
    Inventor: René Cornelis Josephus HOGERS
  • Method for genome sequencing using a sequence-based physical map
    Patent number: 9284606
    Abstract: The present invention relates to a high throughput method for the identification and detection of molecular markers wherein restriction fragments are generated and suitable adaptors comprising (sample-specific) identifiers are ligated. The adapter-ligated restriction fragments may be selectively amplified with adaptor compatible primers carrying selective nucleotides at their 3? end. The amplified adapter-ligated restriction fragments are, at least partly, sequenced using high throughput sequencing methods and the sequence parts of the restriction fragments together with the sample-specific identifiers serve as molecular markers.
    Type: Grant
    Filed: February 4, 2015
    Date of Patent: March 15, 2016
    Assignee: Keygene N.V.
    Inventors: Michael Josephus Theresia Van Eijk, Taco Peter Jesse
  • STRATEGIES FOR HIGH THROUGHPUT IDENTIFICATION AND DETECTION OF POLYMORPHISMS
    Publication number: 20160060686
    Abstract: The invention relates to a method for the high throughput identification of single nucleotide polymorphisms by performing a complexity reduction on two or more samples to yield two or more libraries, sequencing at least part of the libraries, aligning the identified sequences and determining any putative single nucleotide polymorphisms, confirming any putative single nucleotide polymorphism, generating detection probes for the confirmed single nucleotide polymorphisms, subjection a test sample to the same complexity reduction to provide a test library and screen the test library for the presence or absence of the single nucleotide polymorphisms using the detection probe.
    Type: Application
    Filed: October 16, 2015
    Publication date: March 3, 2016
    Applicant: KEYGENE N.V.
    Inventors: Michael Josephus Theresia VAN EIJK, Henricus Johannes Adam VAN DER POEL
  • PEST RESISTANT PLANTS
    Publication number: 20160017364
    Abstract: The disclosure provides an isolated nucleic acid molecule encoding a 7-epizingiberene synthase, a chimeric gene comprising said nucleic acid molecule, vectors comprising the same, as well as isolated 7-epizingiberene synthase proteins themselves. In addition, transgenic plants and plant cells comprising a gene encoding a 7-epizingiberene synthase, optionally integrated in its genome, and methods for making such plants and cells, are provided. Especially Solanaceae plants and plant parts (seeds, fruit, leaves, etc.) with enhanced insect pest resistance are provided.
    Type: Application
    Filed: July 27, 2015
    Publication date: January 21, 2016
    Applicant: Keygene N.V.
    Inventors: Robert Cornelis SCHUURINK, Michael Albertus HARING, Petronella Martina BLEEKER
  • DROUGHT RESISTANCE IN PLANTS: UPL4
    Publication number: 20160010106
    Abstract: The present invention relates to a new method for increasing drought resistance of a plant. The method encompasses the impairment of the expression of a gene or genes in said plant. In comparison to a plant not manipulated to impair the expression of said gene(s), the plants display improved drought resistance. Also provided are plants and plant product that can be obtained by the method according to the invention.
    Type: Application
    Filed: February 18, 2013
    Publication date: January 14, 2016
    Applicant: KEYGENE N.V.
    Inventors: Anne DESLATTES MAYS, Marieke Helena Adriana VAN HULTEN, Shital Anilkumar DIXIT, Martin DE VOS, Jesse David MUNKVOLD, Matthew Vitabile DILEO