Abstract: The present invention relates to a method for phosphorylation site-specific labeling of phosphoproteome with a site-specific tagging reagent and analyzing of the resulting labeled one, more especially, a method for in-situ tagging of phosphorylation sites of phosphoproteins retained in polymeric gel with a nucleophilic tagging reagent. It also relates a method for generating new proteolytic cleavable sites at formerly phosphorylation sites by a proper choice of a nucleophilic tagging reagent. It also relates to a method for phosphopeptides analysis and phosphorylation site identification by in-gel digestion of the previously in-gel tagged proteins and subsequent mass analysis of the resulting peptides. The invention provides in-gel chemical tagging method for phosphoaminoacid residue of phosphoproteins retained in polymeric gel matrix. Phosphoprotein can be immobilized into gel matrix by a variety of methods such as gel electrophoresis.

Abstract: There is provided a method for producing a solid element plasma from a solid lump and a plasma source used in the method. The method of the present invention comprises colliding a solid lump with accelerated particles or lasers to detach solid atoms from the solid lump within a first chamber inside which sputtering of solid atoms is performed, directing the solid atoms to a second chamber inside which plasma discharge is performed, applying a voltage to the second chamber to produce a plasma of solid atoms through plasma discharge, and contacting the plasma of solid atoms to a target to be treated. The present invention avoids problems caused from impurities and poisonous gases of conventional systems adopting a solid element-containing gas as a source of solid element.

Abstract: The present invention relates to the method for tagging of carbohydrates with active methylene compound. Particularly, it relates to the method for tagging of carbohydrates with active methylene compound comprising the step of preparing carbohydrate conjugate in which carbohydrate and methylene compound are combined by mixing carbohydrate mixture and methylene compound under aqueous polar aprotic solvent containing amine base catalyst. The tagging method of the present invention does not need many kinds of chemical reagent and the reactions can be taken even in the presence of certain amount of impurities. So, it can be used for the analysis of oligosaccharide present in the various kinds of samples.

Abstract: Disclosed is a method of analyzing mass of the phosphoproteins or phosphopeptides and of analyzing phosphorylated positions at a phosphoprotein or phosphopeptide, comprising the steps of: 1) dephosphorylating at least one Ser and/or Thr residue of the phosphoprotein or phosphopeptide; 2) tagging the dephosphorylated amino acid residues with a tag having a R-L-G moiety wherein R is a nucleophilic functional group that selectively bind with dephosphorylated amino acid residues, G is selected from the group consisting of guanidine moiety or protected guanidine moiety such as a mono-N-protected guanidino group, a di-N,N?-protected guanidino group and an N?-protected guanidino group, and L is a linker linking the R and the G; and 3) subjecting the tagged proteins or peptides to mass spectrometry. The method is capable of precisely analyzing mass of phosphoproteins of trace amounts as well as positions of phosphoryated amino acids.

Abstract: Disclosed are an apparatus and a method for simultaneously measuring integrated reflectivity of X-rays with different orders of reflections in crystal. Continuous X-rays are incident into the crystal and reflection intensities of the X-rays reflected from the crystal with different orders of reflections are measured based on Bragg's law, thereby measuring reflectivity of X-rays with different orders of reflections.

Abstract: Disclosed is a method of analyzing mass of the phosphoproteins or phosphopeptides and of analyzing phosphorylated positions at a phosphoprotein or phosphopeptide, comprising the steps of: 1) dephosphorylating at least one Ser and/or Thr residue of the phosphoprotein or phosphopeptide; 2) tagging the dephosphorylated amino acid residues with a tag having a R-L-G moiety wherein R is a nucleophilic functional group that selectively bind with dephosphorylated amino acid residues, G is selected from the group consisting of guanidine moiety or protected guanidine moiety such as a mono-N-protected guanidino group, a di-N,N?-protected guanidino group and an N?-protected guanidino group, and L is a linker linking the R and the G; and 3) subjecting the tagged proteins or peptides to mass spectrometry. The method is capable of precisely analyzing mass of phosphoproteins of trace amounts as well as positions of phosphoryated amino acids.

Abstract: A process for preparation of RNA using an affinity column and a DNAzyme is disclosed. By the process, a target RNA can be mass-produced with a high yield, a high resolution and a high purification efficiency. The process includes the steps of preparing a RNA adduct including a target RNA and a tail RNA sequence which is affixed to the 3′-terminal of the target RNA and can complementary combine with an oligo-dN sequence of an oligo-dN affinity column; purifying the RNA adduct by combining the RNA adduct to the oligo-dN affinity column; and obtaining the target RNA by cleaving the purified RNA adduct with a DNAzyme. Preferably, the process for preparation of RNA further includes the steps of removing a RNA fragment cleaved by the DNAzyme by using an affinity column; and decomposing the DNAzyme with a DNA nuclease, and then removing decomposed DNAzyme fragments with a gel filtration column.

Abstract: Disclosed is an X-ray tube for generating X-rays linearly focused in a vertical or horizontal direction in accordance with the orientation of a filament used. The X-ray tube includes a linear filament arranged at a cathode included in the X-ray tube, the linear filament serving to allow an anode included in the X-ray tube to generate line-focused X-rays. The X-ray tube according to the present invention can be applied to a calibration for X-ray spectroscopes, or various applications requiring line-focused X-rays.

Abstract: An improved overvoltage automatic prevention apparatus having individual fault displays and permanent ground functions capable of rapidly and safely protecting load parts of the system from overvoltage caused by a thunderbolt or surge and protecting the communication system by providing automatically operable permanent ground and individual fault display functions when a series of overvoltage is applied thereto, so that easier fault checks, system management, and maintenance is possible, which includes a gas tube connected to subscriber lines and both sides of the electrode surfaces having grooves and having a ground electrode connected to the ground; melting insulation materials inserted into the grooves and defining openings formed at recesses; a permanent ground-side spring connection bar inserted into the openings of the melting insulation materials and receiving a ground electrode of the gas tube and having protruded contact points formed on the inner sides of both connection plates; and a fault display

Abstract: An improved overcurrent automatic prevention apparatus having an individual fault display and permanent ground functions capable of automatically detecting whether overcurrent flows to a switch, which occurs due to a short of communication cables and properly limiting the overcurrent flow thereto, and when overcurrent exceeding a threshold value continuously flows thereto, permanently blocking a cable line and a ground side so as to prevent characteristic changes of a current limiting device and system breakage, and when it is possible to individually display fault states in order to easily check the system by providing an individual fault display device, visually checking a switch, and preventing possible faults in the system, thus increasing the efficiency of the operation, management and maintenance of the communication system, which includes a melting insulation material deposited on the upper surface of an input terminal of the semiconductor; a ground-side spring contact point disposed on the melting ins