Abstract: A system and a method for setting a fluorescence spectrum measurement system for microscopy is disclosed. Using illuminating light (3) from at least one laser that emits light of one wavelength, a continuous wavelength region is generated. Dyes are stored, with the pertinent excitation and emission spectra, in a database of a computer system (23). For each dye present in the specimen (15), a band of the illuminating light (3) and a band of the detected light (17) are calculated, the excitation and emission spectra read out from the database being employed. Setting of the calculated band in the illuminating light and in the detected light is performed on the basis of the calculation. Lastly, data acquisition is accomplished with the spectral microscope (100).

Abstract: A specimen slide unit for holding a specimen that is to be examined under a microscope or analyzed with a laboratory analysis system includes a specimen slide, a data carrier device and a signal element. The data carrier device has a radio frequency identification tag that can be read out and/or written on using Phase Jitter Modulation so as to switch a communication carrier frequency between two predefinable communication carrier frequencies. The signal element can be activated so as to assist an operator in following a predefinable processing sequence including a second specimen slide unit when the RFID tag is in a range of action of the read/write device. The signal element can be a visual or an acoustic signal element.

Abstract: Disclosed is a method for analyzing biological samples by means of a scanning microscope. According to said method, at least one screen dot is repeatedly and successively illuminated with a manipulating light beam and an exciting light beam. The interval between the time of illumination with the manipulating light beam and the time of illumination with the exciting light beam is modified, and the fluorescent light yield is measured in accordance with said interval.

Abstract: A scanning microscope, in particular a confocal scanning microscope, having: a scanning device (1), a detection device (2), an electronic system (3) for operating the scanning microscope, and a cooling device (4) for at least one component of the scanning microscope, is configured and refined, in the interest of efficient cooling, in such a way that the cooling device (4) is operable with a liquid cooling medium.

Abstract: A method and a system for the analysis of co-localizations of dyes present in a specimen. The fluorescence spectra of the dyes present in the specimen are determined. A tolerance region around each of the fluorescence spectra is selected. The spectra of the specimen, in which at least two dyes are present, are then acquired pixel by pixel. Those spectra that lie within the tolerance region around the fluorescence spectra are then calculated. A lambda vector is calculated for each pixel and assigned to a spectrum. Images can be displayed in accordance with the assignment to the spectra.

Abstract: A microscope includes at least one focusing means for focusing an object plane. The focusing means includes first and second adjustment means. The first and second adjustment means are coaxial and independently rotatably adjustable with respect to one another. The second adjustment means are releasably fastenable to the focusing means. In a preferred embodiment, the first adjustment means comprises a coarse focus adjustment knob and the second adjustment means comprises a fine focus adjustment knob. The second adjustment means preferably includes magnetic means adapted for mating engagement with complementary magnetically attractable means of a rotatable focus drive means of the focusing means. A particularly preferred embodiment comprises at least two focusing means wherein the second adjustment means of each may be releasably attached to each; one on each opposite side of the microscope as desired.

Abstract: A scanning microscope having a light source that emits an illuminating light beam, for illumination of a sample. The illuminating light beam extends along an illumination beam path and can be guided over and/or through the sample using a beam deflection device. A mirror which can be introduced in guided fashion into the illumination beam path directs a manipulating light beam via the beam deflection device onto the sample.

Abstract: A method for separating fluorescence spectra of dyes present in a specimen (15) is disclosed. Firstly a spectral scan of the fluorescence spectrum of all the dyes present in the specimen (15) is performed. The fluorescence spectra associated with the respective dyes are stored in a database of the computer system. After spectral deconvolution of the acquired mixed fluorescence spectrum, a comparison is made between the measured individual spectra ascertained by spectral deconvolution and the fluorescence spectra associated with the respective dyes. Lastly, a linear deconvolution of the acquired mixed fluorescence spectrum is performed.

Abstract: A device for adjusting the height of an arrangement 2 relative to a mounting surface 3 is disclosed. Height-adjusting device 1 is designed so that during height adjustment no friction takes place as a result of rotation of the contact sites of the height-adjusting devices 1 with the mounting surface 3. Height-adjusting device 1 consists of a rotary wheel 8, a socket 9 and a foot 11. The height adjustment of rotary wheel 8 and foot 11 relative to the stationary socket 9 occurs in telescopic manner. Height-adjusting device 1 is designed so that the foot 11 undergoes a displacement twice as large as does rotary wheel 8. A support (30) for an optical system is provided with several height-adjusting devices 1 connected with the bottom 5 of a base 31 of the support 30.

Abstract: The invention relates to a scanning microscope comprising a beam deflecting device (11), which directs an illuminating light beam (5) over or through a sample (21), and comprising a detector (33) for receiving detection light (23) exiting the sample. The scanning microscope comprises an extracting port (67) or another detector (37) and comprises a redirecting device (27), which is synchronized with the beam deflecting device and which directs the detection light according to the deflecting position of the beam deflecting device either to the detector or to the extracting port or to the other detector.

Abstract: An inspection microscope for several wavelength ranges with at least one illuminating beam path and at least one imaging beam path. Those optical elements in the illuminating beam path and in the imaging beam path, through which beams of all wavelengths pass, are provided with a reflection-reducing layer, by means of which the wavelength ranges with reduced reflection are the visible VIS-wavelength range up to 650 nm, the i-lines at ?=365 nm and the ultraviolet DUV-wavelength range from 240 nm to 270 nm. The reflection-reducing layer is a sandwich structure, comprising various material combinations, such as for example, M2/MgF2 or M2/MgF2/SiO2 or M2/MgF2/Al2O3, where M2 is a mixed substance from the company Merck, comprising Al2O3. The optical components with reduced reflectance preferably comprise quartz glass or CaF2.

Abstract: A device and method for inspecting an object (2) uses a bright field illumination beam path (4) of a bright field light source (5), said beam path being formed so that it passes through the projection optics (3), and a dark field illumination beam path (6) of a dark field light source (7), this beam path being formed so that it also passes through the projection optics (3). The object (2) can be projected by the projection optics (3) onto the least one detector (8), and the object (2) is simultaneously illuminated by both light sources (5, 7). In order to simultaneously detect bright field images and dark field images without involving complicated filtering operations, the light used for the dark field illumination is pulsed and the pulse intensity of the light used for the dark field illumination is greater by at least one order of magnitude than the intensity of the continuous light, which is used for the bright field illumination, during a pulsed interval.

Abstract: A microscope objective includes an objective sleeve including a first and a second sub-sleeve. A first lens is received in a first mounting ring, a second lens is received in a second mounting ring, a third lens is received in a third mounting ring, and a fourth lens is received in a fourth mounting ring. The first and second mounting rings are received in the first sub-sleeve and the third and fourth mounting rings are received in the second sub-sleeve.

Abstract: A detection device for an optical configuration, in particular for a confocal microscope, with a detector, a pinhole aperture 2 arranged before the detector in a beam path 1 of the detection light, and a lens 3 for focusing the detection light on the pinhole aperture 2 arranged before the pinhole aperture 2, is implemented with regard to high resolution and detection efficiency even in the presence of varying wavelengths such that a means 4 for wavelength-dependent illumination of the lens 3 is arranged before the lens 3 in the beam path 1. Furthermore, a wavelength-dependent beam expander 6 as well as a confocal microscope with such a detection device are disclosed.

Abstract: A microscope includes an eyepiece, an illumination beam path for illuminating a specimen, and a detection beam path for imaging the specimen. A microscope operator interface is also provided, as well as an image overlay device for overlaying an image of the microscope operator interface onto the detection beam path so that the image of the microscope operator interface together with an image of the specimen is presented at the eyepiece. Also provided is at least one operating apparatus for interacting between the operator and the microscope operator interface.

Abstract: An illumination apparatus for an optical system is disclosed, the illumination apparatus (1) encompassing a single light source (2) that emits broad-band light into an illumination beam path (2a). Placed after the light source (2) in accordance with the present invention is a filter system (4), with which several wavelengths or wavelength regions are separable and are guided through a light-guiding means (22) to an optical system (5). The filter system possesses several reflecting filters (8a, 8b, 8c, 8d) which are arranged in such a way that a selected wavelength is guidable by way of those reflection filters and leaves the filter system (4) parallel to the illumination beam path (2a).

Abstract: A microscope includes a microscope arm having an interior and an exterior surface. The interior surface has at least one recessed portion disposed therein extending from the interior surface of the microscope arm to an inner recess surface of the microscope arm. The recessed portion forms a surface for gripping the microscope. A portion of the recessed portion is, preferably, disposed above the stage and below the objective turret of the microscope. The recessed portion may comprise a textured surface for increasing the static coefficient of friction thereof.

Abstract: An apparatus for detecting photons of a light beam emanating from a spatially limited source includes a detection device having a plurality of detectors forming a three-dimensional array with semitranslucent EMCCDs disposed one behind another. A light splitting device is disposed in a path of rays of the light beam for splitting the light beam so as to distribute the photons over the detectors for detection.

Abstract: A spectral scanning microscope and a method for data acquisition using a spectral scanning microscope are disclosed. A computer system is provided that encompasses a memory and a database. In combination with the computer system and/or the database, a continuous wavelength subregion that serves to illuminate the specimen can be selected from a continuous wavelength region using the spectral selection means. Also in combination with the computer system together with the spectral selection means, a detection band can be selected from the detected light beam.

Abstract: An arrangement for micromanipulation of biological specimens includes a microscope, a motor-adjustable micromanipulator, and a common operating console having a motor-adjustable microscope functional element. The motor-adjustable micromanipulator has an injector. The common operating console includes a first operating element and a second operating element. Each of the first and second operating elements are selectably configurable to independently operate either of the motor-adjustable microscope functional element and the motor-adjustable micromanipulator.