Abstract: An optical measurement system having a spectrophotometer and a ellipsometer is calibrated, the spectrophotometer and the ellipsometer first being calibrated independently of one another. A spectrophotometer layer thickness (dphoto) of a specimen is then determined at an initial angle of incidence (?init) using the spectrophotometer. An ellipsometer layer thickness (delli) of the specimen is then determined using the layer thickness determined with the ellipsometer. The spectrophotometer and the ellipsometer are matched to one another by varying the initial angle of incidence (?init) until the absolute value of the difference between the spectrophotometer layer thickness (dphoto) and the ellipsometer layer thickness (delli) is less than a predefined absolute value.

Abstract: A microscope includes an interference contrast transmitted-light device having an analyzer disposed in the microscope imaging beam path, the analyzer causing a beam deflection. A fluorescence device is provided, the fluorescence device and the interference contrast transmitted-light device being selectably and alternatively insertable into the imaging beam path. A pair of glass wedge plates are arranged behind the analyzer in the imaging direction so as to compensate to zero for the beam deflection caused by the analyzer.

Abstract: A scanning microscope for imaging an object includes a light source and a spectrally selective detection device. An illumination beam path extends from the light source to the object. A detection beam path extends from the object to the detection device. A spectrally selective element useable to select light from the light source is provided. The spectrally selective element is useable to mask out of the detection beam path the selected light from the light source reflected or scattered on the object. An illumination slit diaphragm is disposed in the illumination beam path and configured to generate a linear illumination pattern in a region of the object. A detection slit diaphragm is disposed in the detection beam path and configured to detect the light coming from the linear illumination region from a focal plane so as to provide a confocal slit scanner.

Abstract: The arrangement for spectrally sensitive reflected-light and transmitted-light microscopy comprises an illuminating device which illuminates a sample under study in two dimensions. A scanning device is provided which respectively directs the light emanating from a detection region of the sample under study onto a multiband detector. In one embodiment, the illuminating device is arranged in such a way that it transilluminates the sample in two dimensions. In a further embodiment, the illuminating device illuminates the sample in two dimensions in reflected light.

Abstract: A comparison optical system (1) having an illumination system for specimens, the comparison optical system (1) being equipped with several macroscopes (2a, 2b). The macroscopes (2a, 2b) are connected to one another via a bridge (3). Each macroscope (2a, 2b) is connected to an attachment device (25a, 25b) for the illumination system. The attachment device (25a, 25b) is constructed from several movable arms (30, 32, 35) and an adjustable holder (37) for a light source.

Abstract: Many points on a sample are tested simultaneously in parallel in an FCS method with multifocal illumination and/or detection.

Abstract: An optical arrangement for a microscope, in particular for a scanning microscope, with a beam splitter 1 arranged in a divergent and/or convergent beam path for separating an illumination light 2 that is produced by an illumination source from a detection light 3 that is emitted by a sample being tested, which with regard to reliable correction of imaging errors can be implemented and developed even when using thick beam splitters 1 such that the beam splitter 1 is wedge-shaped and implemented as a beam splitter plate for reflection primarily at a glass-air interface 4. Furthermore, a microscope with such an optical arrangement is disclosed.

Abstract: A lamp assembly for a microscope, the microscope having a stand, comprising a female member comprising a first electrical socket, the socket arranged to provide a source of electricity for a lamp, the female receptacle located inside the microscope stand, and, a male member comprising at least two electrical pin terminals arranged to matingly engage the first electrical socket to receive electricity therefrom, and, a second socket electrically connected to the at least two electrical pin terminals, the second socket arranged to hold the lamp and provide electricity thereto.

Abstract: The invention relates to a device, such as a rotary disk (1) or a longitudinal slider (2), for retaining optical components, this device comprising open orifices (3) arranged next to one another, and a raceway (4) having detents (5) being provided in the direction of the arranged orifices (3), which detents are embodied in such a way that they are to be brought into click-stop engagement with a ball bearing (6) running along the raceway (4), in order to hold the device (1, 2) in a selected position. In order to enable gentle click-stopping of the ball bearing (6) with a wide capture region, it is proposed that the detents (5) have, in the running direction, a profile comprising a combination of two arc-shaped profiles (10, 11), such that an outer profile (11) of lesser curvature surrounds an inner profile of greater curvature (10).

Abstract: The invention relates to a device for examining and manipulating microscopic objects with a microscope having a light source that serves to illuminate the object, and which generates an illumination light beam that runs along and illumination beam path, that can be guided over or through the object by means of a beam deflector, with a detector to detect light emitted from the object that runs along the detection beam path, with a primary beam splitter, and with a light source, which generates a manipulation light beam that runs along an illumination beam path, that serves to manipulate the object.

Abstract: The invention concerns a method and an apparatus for investigating layers (1) of tissues in living animals using a microscope (2). The microscope (2) is focused onto a layer (1), and images of the layer (1) are acquired or optical measurements are performed on it. Positional changes of the layer (1) are brought about by movements of the animal or of its organs. The positional changes are sensed, and corresponding signals are generated. The signals are stored, together with the corresponding images or measurement results, for later evaluation; or they are processed in such a way that the positional changes are compensated for in order to investigate the layer (1). As a result, the layer (1) can be qualitatively or quantitatively investigated microscopically, irrespective of the movement of the animal or its organs.

Abstract: A detection device for an optical configuration, in particular for a confocal microscope, with a detector, a pinhole aperture 2 arranged before the detector in a beam path 1 of the detection light, and a lens 3 for focusing the detection light on the pinhole aperture 2 arranged before the pinhole aperture 2, is implemented with regard to high resolution and detection efficiency even in the presence of varying wavelengths such that a means 4 for wavelength-dependent illumination of the lens 3 is arranged before the lens 3 in the beam path 1. Furthermore, a wavelength-dependent beam expander 6 as well as a confocal microscope with such a detection device are disclosed.

Abstract: An optical arrangement includes a laser, and a chromatic error correcting device arranged in a beam path of the laser. The chromatic error correcting device includes a pulse stretcher.

Abstract: An illumination device in a microscope includes multiple light sources arranged on a mirror housing. A mirror is provided which is rotatably or displaceably arranged in the mirror housing for selectably switching each of the light sources into an illumination beam path. At least one drive system is provided for rotating or displacing the mirror so as to switch one of the light sources at a time into the illumination beam path.

Abstract: An EMCCD detector includes a first gain register and a photoactive area divided into at least a two detection regions. At least one of the detection regions is assigned to the first gain register.

Abstract: A microscope (1), preferably a confocal laser scanning microscope, having at least one light source, a detector, and two objectives (2), one of the objectives (2) being arranged on each of the two sides of the specimen plane (3) and the objectives (2) being directed toward one another and having a common focus; and at least one beam splitter (5) for distributing the illuminating light (6) to the objectives (2), and a beam recombiner (5) for combining the detected light (7) coming from the objectives (2), being provided in the illumination/detection beam path (4), is characterized, for selectable, subsequent implementation of ultrahigh-resolution microscope techniques, in that the objectives (2) and the beam splitter/beam recombiner (5) are grouped into a modular assembly (8); and the assembly (8) has an interface (9) for connection to the illumination/detection beam path (4) of the microscope (1).

Abstract: An apparatus for selecting and detecting at least two spectral regions of a light beam includes a selection device, a detection device, and a light suppression device. The light suppression device suppresses reflected and/or scattered light of an illuminating light of a microscope. The light suppression device is located in an optical path between the selection device and the detection device, and includes a graded filter.

Abstract: An auto focus method for a microscope (2), and a system for adjusting a focus for the microscope (2), are disclosed. The microscope (2) possesses a microscope stage (18) and an objective (16) located in a working position. A relative motion in the Z direction takes place between the microscope stage (18) and the objective. Images are read in by the camera (20) during the relative motion, and a microscope control device (4) and a computer (6) are provided for evaluation and determination of the focus position.

Abstract: A portable microscope and a microscope stage (10) for a portable microscope are disclosed. The microscope comprises a stand (4) that carries the adjustable-height microscope stage (10). The microscope stage (10) is constructed from a sample support part (38), a guide part (40), and a base (42). The base (42) of the microscope stage (10) carries an illumination module (39).