Abstract: A scanning microscope with a first and at least one other detection channel is disclosed. The first detection channel comprises at least one first detector and the other detection channel comprises at least one other detector to detect the detection light given off by a sample. A switching mechanism is provided that selectively directs the detection light into the first or into the other detection channel.

Abstract: A microscope objective includes an objective sleeve including a first and a second sub-sleeve. A first lens is received in a first mounting ring, a second lens is received in a second mounting ring, a third lens is received in a third mounting ring, and a fourth lens is received in a fourth mounting ring. The first and second mounting rings are received in the first sub-sleeve and the third and fourth mounting rings are received in the second sub-sleeve.

Abstract: A method for superimposing optical information in a scanning microscope includes determining a transformation matrix, and superimposing first optical information of a CCD image and second optical information of at least one piece of second image information using the transformation matrix.

Abstract: The present invention concerns a method and an arrangement for imaging and measuring microscopic three-dimensional structures. In them, a data set is depicted in three-dimensional form on a display (27) associated with the microscope. At least one arbitrary section position and an arbitrary rotation angle are defined by the user. Rotation of the three-dimensional depiction on the display (27) is performed until a structure contained in the three-dimensional form reproduces on the display (27) a depiction that appears suitable to the user. The corresponding analytical operations are then performed on the structure.

Abstract: A method for investigating transport processes in a preferably biological specimen, a laser light beam (2, 3) being guided by means of a scanning apparatus line by line over the specimen within definable specimen regions, and the light proceeding from the specimen being detected by means of a detection apparatus, is characterized, in the interest of the capability of investigating, with high accuracy, processes within the specimen that proceed on a short time scale, in that both an image production light beam (5) for the purpose of observing the specimen and a manipulation light beam (4) for the purpose of manipulating the specimen are used as the laser light beam (2, 3), the image production light beam (5) preceding the manipulation light beam (4) in such a way that pixels of the specimen not yet manipulated by the manipulation light beam (4) are illuminated with the image production light beam (5).

Abstract: An illumination apparatus for an optical system, in particular for a microscope, having a light source (2) emitting preferably broad-band light in order to furnish an output light beam (3), and having a filter device (5), is characterized, in the interest of economical, low-maintenance, and vibration-free generation of an illuminating light beam encompassing multiple wavelengths or wavelength regions, and a high degree of flexibility in terms of the spectral light shaping of the illuminating light beam, in that the filter device (5) encompasses at least one means (7) for spatial splitting of the output light beam (3) into light sub-beams (8, 9) and at least one means for spectral manipulation of at least one of the light sub-beams (8, 9); and that at least one beam combining means is provided with which definable light sub-beams (9) are combinable into one illuminating light beam (14) for the optical system.

Abstract: The scanning microscope includes an illumination beam path, microscope optics and at least one light source which generates an excitation light beam of a first wavelength and a second light beam of a second wavelength. Microscope optics are provided for focussing the excitation light beam onto a first focal region in a first plane of a sample and for focusing the second light beam onto a second focal region in a second plane of the sample. The first focal region and the second focal region overlap partially. The optical properties of the components arranged in the illumination beam path are matched to one another such that optical aberrations are corrected in such a way that the focal regions remain static relative to one another irrespective of the scanning movement.

Abstract: The present invention concerns a method, an arrangement, and a software program for controlling analytical and adjustment operations of a microscope. The arrangement has an electronic acquisition system (50) which converts the electrical signals coming from the detectors (19) into digital signals and preprocesses them. A PC (34) receives the digital signals from the electronic acquisition system (50) and identifies from the digital signals a graphical depiction. On a display (27), the graphical depiction is displayed and moreover the user is offered the opportunity to select adjustment functions. An input unit (33), with which selection of the adjustment functions and selection of at least one structure of interest can be achieved, is provided for selection. An electronic control system (67), with which the adjusting elements of the microscope can be controlled, is connected to the PC (34).

Abstract: The invention concerns an apparatus for beam deflection, in particular for scanning microscopy, a light beam being deflectable by a mirror arrangement that is alternatingly rotatable by a rotary drive. The apparatus for beam deflection makes possible maximum variability in terms of frequency range and maximally achievable oscillation frequency, and is thus usable in flexible and versatile fashion. It moreover allows almost any desired angular offset from the zero point position to be established, and is characterized in that the rotary drive comprises two mutually independent drive units which rotate the mirror arrangement, together or mutually independently, about a rotation axis.

Abstract: A microscope (1) having a holding apparatus, insertable reversibly into the microscope (1), for interchangeable optical components is described. According to the present invention, the holding apparatus is embodied as a pivoting drawer (10) that is arranged pivotably in and out about a pivot axis. During exchange or insertion of optical components, the pivoting drawer (10) always remains connected to the microscope stand (2) even in the pivoted-out state. It is guided while being pivoted in, so that jamming, as in the case of plug-in modules previously known from the existing art, is avoided.

Abstract: A microscope (10) is disclosed, having a revolving nosepiece (12) on which are mounted multiple objectives. The objectives (11) comprise multiple components 211, . . . 21n). A light-intensity-reducing layer (40) is applied onto at least one component of an objective. The light-intensity-reducing layer (40) is configured in such a way that for each objective introduced into the illumination beam path, the brightness behind it is of the same magnitude.

Abstract: An inverted microscope (1) equipped with an ergotube (9) is disclosed. The ergotube (9) substantially comprises an eyepiece (9a) and a tube housing (9b). The microscope beam path (30) defined by the inverted microscope (1) is of U-shaped configuration. The U-shaped microscope beam path (30) is deflected into the eyepiece (9a) by a single deflection element (74) that is embodied as a pivotable mirror.

Abstract: A slider for positioning multiple optical elements is disclosed. Also disclosed is a microscope that contains the slider (25) according to the present invention. The slider (25) encompasses a slider carrier (35) that is guided on a first shaft (31) and a second shaft (32). The second shaft (32) is equipped with multiple detent positions (34) so as thereby to position the slider (25) exactly in the optical axis (20) of the microscope (1). The slider carrier (35) is guided on the second shaft (32) by a first roller (36) and a second roller (38). The second roller (38) is arranged below the center axis of the second shaft (32).

Abstract: A microscope (1) with manual and external adjustability of an optical element (41) is disclosed. The optical element (41) is displaceable into an optical axis (20) determined by the microscope (1). The microscope (1) further encompasses a stand (3) and a tube (9) attachable to the stand (3). The optical element (41) is arranged on an adjustment element (45) in the interior of the stand (3). Provided in the interior of the stand (3) is a closure element (26) that enables access to the adjustment element (45) when the optical element (41) is positioned in the optical axis (20) of the microscope.

Abstract: An indexable microscope is described, that comprises a microscope stand and an optical beam path, having a plurality of optical components that are switchable into and out of the beam path, which are arranged on at least two independent mechanical assemblies to be indexed separately and are switchable selectably into and out of the beam path by indexing the respective assembly. According to the present invention, a space-saving and flexible arrangement of the separately indexable assemblies having the optical components is achieved in that a common central receiving plate is provided, on the upper side and the lower side of which at least one of the assemblies is respectively arranged. Configuration of the assemblies as rotary disks permits very flexible arrangements which allow both manual and motorized actuation of the indexing of the assemblies.

Abstract: An optical device exhibits a focusing optic that focuses a light beam in a focal plane. A means is envisaged to form the focus of the light beam, whereby the means to form the focus brings about a phase shift between a first part of the light beam and a second part of the light beam. The optical device is characterized in that the means to form the focus exhibits a first interface and a second interface, wherein the first interface reflects the first part of the light beam, and the second interface reflects the second part of the light beam.

Abstract: A microscope for investigating the lifetime of excited states in a sample has a light source that generates excitation light, and has a detector that receives detected light proceeding from the sample. The light source contains a semiconductor laser which emits pulsed excitation light. An adjusting apparatus is provided for adjusting the pulse repetition rate to the specific lifetime properties of the sample.

Abstract: The scanning microscope includes an illumination beam path, microscope optics and at least one light source which generates an excitation light beam of a first wavelength and a second light beam of a second wavelength. Microscope optics are provided for focussing the excitation light beam onto a first focal region in a first plane of a sample and for focusing the second light beam onto a second focal region in a second plane of the sample. The first focal region and the second focal region overlap partially. The optical properties of the components arranged in the illumination beam path are matched to one another such that optical aberrations are corrected in such a way that the focal regions remain static relative to one another irrespective of the scanning movement.