Abstract: A method for determining a neural network for correcting optical aberrations includes determining one or more images that are at least partly related to an optical system or the design of an optical system. A neural network is determined on the basis of the determined one or more images in such a way that the determined neural network when applied to an image captured by the optical system outputs an image which has been corrected in relation to one or more optical aberrations.

Abstract: A method for determining a focus position includes recording at least one first image, wherein image data of the at least one recorded first image are dependent on at least one first focus position during the recording of the at least one first image. A second focus position is determined based on an analysis of the at least one recorded first image using a trained model. At least one second image is recorded using the second focus position. The at least one first image and the at least one second image contain items of information which are in a context with a training of the trained model.

Abstract: A method is useable for determining a thickness of a cover slip or object carrier in a microscope, which has an objective facing toward a sample chamber. Two optical media border two opposing surfaces of the cover slip or object carrier and form two partially reflective interfaces, which are arranged at different distances from the objective. The method includes: deflecting a measurement light beam by the objective with oblique incidence on the cover slip or object carrier; generating two reflection light beams spatially separated from one another by the measurement light beam being partially reflected on each of the two interfaces; receiving the two reflection light beams by the objective and conducting them onto a position-sensitive detector; registering the incidence locations on the position-sensitive detector; and determining the thickness of the cover slip or object carrier based on the registered incidence locations.

Abstract: Embodiments relate to a system (100) comprising one or more processors (110) and one or more storage devices (120). The system (100) is configured to receive biology-related language-based search data (101) and generate a first high-dimensional representation of the biology-related language-based search data (101) by a trained language recognition ma-chine-learning algorithm executed by the one or more processors (110). The first high-dimensional representation comprises at least 3 entries each having a different value. Further, the system is configured to obtain a plurality of second high-dimensional representations (105) of a plurality of biology-related image-based input data sets or of a plurality of biology-related language-based input data sets and compare the first high-dimensional representation with each second high-dimensional representation of the plurality of second high-dimensional representations (105).

Abstract: A laboratory system includes: a laboratory device on a table surface of a laboratory bench, the laboratory bench having at least one receiving section below the table surface, which at least one receiving section receives at least one fluid reservoir which is connected to the laboratory device. In an embodiment, the laboratory device includes a climate chamber and/or an incubation system, and the laboratory system further includes a first fluid supply system that supplies at least one fluid to the climate chamber and/or the incubation system, the first fluid supply system being connected to the at least one fluid reservoir in which the at least one fluid is storable.

Abstract: An optical assembly includes a lens unit capable of being moved along an optical axis of the optical assembly. The lens unit includes a lens mount for holding at least one lens. The optical assembly further includes a sleeve for receiving the lens mount. The lens mount is in sliding contact with and movable in relation to the sleeve as the lens unit is moved along the optical axis. The lens mount is formed of or comprises at its outer surface a self-lubricating material, and/or the sleeve is formed of or comprises at its inner surface a self-lubricating material.

Abstract: A microscope system includes: at least one microscope component with electrically adjustable component parameters; a controller for generating electrical signals and transmitting the electrical signals to the at least one microscope component to set the electrically adjustable component parameters; and a computer having at least one display unit, the computer generating an input unit having a graphical user interface on the at least one display unit, at least three setting parameters being adjustable by the graphical user interface. The input unit has an input pointer that is positionable in an input area so as to set a respective value for the at least three setting parameters. A coordinate axis is defined in the input area for each of the at least three setting parameters, with at least one coordinate axis not being perpendicular to another coordinate axis. Values of the at least three setting parameters are determined by the coordinates.

Abstract: The invention relates to a microscope for microscopic examination of a sample comprising a microscope housing enclosing an illumination optics, a microscope stage and an imaging optics, an integrated sample chamber located within the microscope housing and formed by a separated housing section within the microscope housing. The housing section has a lid which provides direct access to the microscope stage for placing the sample in the sample chamber. The housing section has an interface for connection of an external incubation environment conditioning unit to the sample chamber. The interface is configured to provide a connection between the external incubation environment conditioning unit and the sample chamber, such that the environmental conditions in the sample chamber can be controlled when the external incubation environment conditioning unit is connected to the interface.

Abstract: An optical system for a microscope for imaging an object includes: a telescope system having an optical correction unit, which is adjustable in order to correct a spherical imaging aberration, and having a zoom optical unit, which is adjustable in order to adapt a magnification of the telescope system to a ratio of two refractive indices, one of which is assigned to an object side and an other of which is assigned to an image side, within a predetermined magnification range. The telescope system is telecentric over an entire magnification range both with respect to the object side and with respect to the image side by the zoom optical unit contained in the telescope system.

Abstract: A method for approximating image data is disclosed. The method includes obtaining initial image data formed by imaging at least part of a sample and analysing the initial image data to form one or more portions of image data. The method also includes accessing a set of predefined stereotype elements, determining one or more of the stereotype elements as a visual approximation for the at least one object within the sample, selecting one or more of the determined stereotype elements to be associated with at least one object within the sample, and forming instruction data based on and/or including the one or more selected stereotype elements, the instruction data including instructions on how to arrange the one or more selected stereotype elements, to a processing system for approximating image data and to a system comprising such processing system.

Abstract: An apparatus for reducing a chromatic spread angle of light diffracted at an acousto-optic element includes the acousto-optic element and a first and a second focusing optical unit. The acousto-optic element is disposed in a beam path of an incident light beam and is configured to generate the diffracted light from the incident light beam such that the diffracted light emanates from a virtual interaction point of the acousto-optic element. The first focusing optical unit is disposed in the beam path upstream of the acousto-optic element and the second focusing optical unit is disposed in the diffracted light such that a focus of the incident light beam is situated downstream of the first focusing optical unit in the acousto-optic element and the virtual interaction point is located in a front focus of the second focusing optical unit.

Abstract: A microscope system for imaging at least one region of a sample includes: an image generating unit for microscopic imaging of a section of a sample region to be imaged acquired in an observation beam path; a movement unit for moving the section to be imaged into the observation beam path of the image generating unit; a graphic user interface displayed on a display for establishing a movement range corresponding to the sample region to be imaged by way of at least one user input, the graphic user interface displaying a coordinate system and, after input of at least one point in the displayed coordinate system, the movement range is established as a defined movement volume; and a control unit for activating the movement unit as a function of the defined movement volume so as to approach a set of sections corresponding to the defined movement volume in succession.

Abstract: A method for manipulating at least one beam path in a microscope includes ascertaining a refractive index of a sample arranged in a sample volume and/or of an optical medium arranged in the sample volume. At least one microscope parameter is set in dependence on the ascertained refractive index for manipulating the beam path.

Abstract: A light source module for a microscope, including a light source configured to emit illumination light along an illumination light path, at least one light blocking shutter configured to be moved into and out of the illumination light path, and at least one light sensor configured to detect an intensity of the illumination light propagating along the illumination light path. The at least one light sensor is integrated with the at least one light blocking shutter to be moved therewith into and out of the illumination light path.

Abstract: A method for laser microdissection includes: processing a microscopic examination object by a laser beam using tuples of coordinate values which respectively indicate positions of target points on the examination object at least in a first spatial direction and a second spatial direction orthogonal to the first spatial direction, positions of at least three reference points being ascertained beforehand in each case in the first and second spatial directions and also in a third spatial direction orthogonal to the first and second spatial directions; defining a reference plane based on the positions of the reference points; and determining, for the target points, further coordinate values indicating an expected position of the target points on the examination object in the third spatial direction in each case, as determined further coordinate values, the determining of the further coordinate values being performed depending on the defined reference plane.

Abstract: An oblique plane microscope has an objective illuminating a plane of the sample and collecting detection light. A beam splitting system splits the collected detection light into two detection light bundles along two optical paths, respectively. A first intermediate imaging system generates a first intermediate image of the plane in a first intermediate image space. The first intermediate imaging system has a first objective and a first reflecting element in the first intermediate image space and reflecting the first detection light bundle into the first objective. A second intermediate imaging system generates a second intermediate image of the plane in a second intermediate image space. The second intermediate imaging system has a second objective and a second reflecting element positioned in the second intermediate image space and reflecting the second detection light bundle into the second objective. A detection system detects the detection light bundles reflected into the objectives.

Abstract: A method for estimating baseline in a signal, the signal being represented by input signal data (I(xi)), includes estimating a baseline contribution (I2(xi)) in the signal to obtain baseline estimation data (f(xi)), wherein the baseline estimation data (f(xi)) are computed as a fit to at least a subset of the input signal data (I(xi)) by minimizing a least-square minimization criterion (M(f(xi))). Deblurred output signal data (O(xi)) are obtained based on the baseline estimation data (f(xi)) and the input signal data (I(xi)). The least-square minimization criterion (M(f(xi))) comprises a penalty term (P(f(xi))).

Abstract: A method for estimating a stimulated emission depletion microscopy (STED) resolution includes generating a first frame representing a reference image from a field-of-view, the reference image having a predetermined reference resolution, and generating at least one second frame representing a STED image from the same field-of-view, the STED image having the STED resolution to be estimated. The at least one second frame is blurred by applying a convolution kernel with at least one fit parameter to the second frame. An optimal value of the at least one fit parameter of the convolution kernel is determined for which a difference between the first frame and the blurred at least one second frame is minimized. The STED resolution is estimated based on the optimal value of the at least one fit parameter and the predetermined reference resolution.

Abstract: An optical imaging device for a microscope comprises a first optical system configured to form a first optical image corresponding to a first region of a sample in accordance with a first imaging mode, a second optical system configured to form a second optical image corresponding to a second region of said sample, wherein said first and second regions spatially coincide in a target region of said sample and said first and second imaging modes are different from each other, a memory storing first distortion correction data suitable for correcting a first optical distortion caused by said first optical system in said first optical image, second distortion correction data suitable for correcting a second optical distortion caused by said second optical system in said second optical image, and transformation data suitable for correcting positional misalignment between said first and second optical images, and a processor which is configured to process first image data representing said first optical image based