Patents Assigned to Leica Microsystems CMS GmbH
  • Publication number: 20210278651
    Abstract: A light sheet microscope includes: a detection objective configured to image a target region of a sample located in a focal plane of the detection objective; an illumination objective configured to focus an illumination light beam in the sample, the detection objective and the illumination objective being opposite to one another, and the optical axis of the detection objective and the optical axis of the illumination objective being parallel to one another; a first light deflection device having at least one first deflection surface and one second deflection surface, which are each arranged offset to the optical axis of the detection objective and are configured to deflect the illumination light beam focused by the illumination objective in a direction perpendicular to the optical axis of the detection objective such that a deflected illumination light beam forms a light-sheet-like illumination light distribution focused in the focal plane.
    Type: Application
    Filed: November 12, 2019
    Publication date: September 9, 2021
    Applicant: Leica Microsystems CMS GmbH
    Inventors: Werner KNEBEL, Florian FAHRBACH, Christof FRANKE, Dirk-Oliver FEHRER
  • Patent number: 11086117
    Abstract: An apparatus for light-sheet-like light illumination of a sample includes a light source configured to generate an illumination beam. A focusing system is configured to focus the illumination beam to form a light-sheet-like illumination light distribution, with which a focal plane of the sample can be illuminated. An imaging optical unit is configured to image the light-sheet-like illumination light distribution into the focal plane. A polarization element, arranged in a position conjugated to the focal plane between the focusing system and the imaging optical unit, is configured to split the illumination beam into two differently polarized sub-beams, which propagate into the imaging optical unit in different propagation directions, whereby the light-sheet-like illumination light distribution can be imaged by the imaging optical unit in the form of two differently polarized light-sheets, which from a same side of the focal plane are superimposed on each other in the focal plane.
    Type: Grant
    Filed: May 3, 2017
    Date of Patent: August 10, 2021
    Assignee: Leica Microsystems CMS GmbH
    Inventor: Christian Schumann
  • Publication number: 20210116695
    Abstract: A microscope control unit includes at least one connection which is connectable to one or more electrically addressable microscope components or other electrically controllable components. At least one connection parameter of the at least one connection is programmatically configurable. At least one script with script commands is provided on the microscope control unit. At least one terminal is addressable by the script commands.
    Type: Application
    Filed: April 26, 2018
    Publication date: April 22, 2021
    Applicant: Leica Microsystems CMS GmbH
    Inventors: Markus Weber, Robert Mainberger
  • Publication number: 20200386616
    Abstract: A method for counting photons using a photomultiplier includes obtaining a measurement signal from a raw signal produced by the photomultiplier by correcting the raw signal for a noise signal and/or an offset, wherein an incident photon produces a pulse in the raw signal. The measurement signal is integrated over time to form an analog integrated measurement signal. A number of photons that are incident in the photomultiplier is ascertained by comparing a value of the analog integrated measurement signal to an integral proportionality value which corresponds to a specific number of photons incident in the photomultiplier.
    Type: Application
    Filed: August 28, 2018
    Publication date: December 10, 2020
    Applicant: Leica Microsystems CMS GmbH
    Inventors: Holger BIRK, Bernd WIDZGOWSKI
  • Patent number: 10768399
    Abstract: The invention relates to a mirror device for deflecting illuminating light in SPIM microscopy. The invention is characterized by a holding component that comprises a connecting element for mounting the holding component on a microscope objective, at least one deflection mirror being detachably mounted on the holding component.
    Type: Grant
    Filed: September 25, 2015
    Date of Patent: September 8, 2020
    Assignee: Leica Microsystems CMS GmbH
    Inventors: Ingo Paulus, Sebastian Hitzler
  • Patent number: 10591356
    Abstract: The invention relates to a beam combiner for a microscope, in particular a scanning microscope, which receives at least a first illuminating light bundle and a second illuminating light bundle and combines them into a collinear output light bundle, the first illuminating light bundle and the second illuminating light bundle having the same illuminating light wavelength but a different polarization, in particular linear polarization. The beam combiner is embodied as an acousto-optic beam combiner and is constructed and operated in such a way that by interaction with at least one mechanical wave, both the first illuminating light bundle and the second illuminating light bundle are diffracted and are thereby directed into a common optical axis.
    Type: Grant
    Filed: September 3, 2014
    Date of Patent: March 17, 2020
    Assignee: Leica Microsystems CMS GmbH
    Inventors: Volker Seyfried, Vishnu Vardhan Krishnamachari
  • Patent number: 10458899
    Abstract: The invention relates to a method for microscopic investigation of a plurality of samples.
    Type: Grant
    Filed: June 18, 2014
    Date of Patent: October 29, 2019
    Assignee: Leica Microsystems CMS GmbH
    Inventors: Werner Knebel, Wernher Fouquet, Frank Sieckmann
  • Patent number: 10429301
    Abstract: The invention relates to a method for tomographic investigation of a sample, in which method a sample is illuminated with an illuminating light bundle and in which a transmitted light bundle that contains the light of the illuminating light bundle transmitted through the sample is detected with a transmission detector. The invention further relates to an apparatus for tomographic investigation of a sample. Provision is made that the illuminating light bundle and the transmitted light bundle pass in opposite propagation directions through the same objective.
    Type: Grant
    Filed: December 17, 2014
    Date of Patent: October 1, 2019
    Assignee: Leica Microsystems CMS GmbH
    Inventors: Werner Knebel, Wernher Fouquet, Frank Sieckmann
  • Patent number: 10429630
    Abstract: A fluorescence microscope has an incident fluorescence illumination unit generating an incident fluorescence illumination beam path a multi-band fluorescence filter system encompassing a multi-band beam splitter for deflecting the incident fluorescence illumination beam path into an objective of the microscope and onto a specimen. The filter system has a multi-band blocking filter that at least partly transmits a fluorescence emission beam path proceeding from the specimen; and having a digital camera for generating a fluorescence image of the specimen. The microscope has a bright-field transmitted illumination unit for generating a bright-field transmitted illumination beam path for transmitted illumination of the specimen. The filter system is arranged fixedly so that upon acquisition of a bright-field image of the specimen, the system remains in a bright-field transmitted light beam path proceeding from the specimen.
    Type: Grant
    Filed: October 28, 2016
    Date of Patent: October 1, 2019
    Assignee: Leica Microsystems CMS GmbH
    Inventors: Benjamin Deissler, Christian Schumann
  • Publication number: 20190278072
    Abstract: An illumination apparatus for a microscope, for producing a de-excitation or switching light distribution, includes a light source configured to produce a primary illumination light beam and a beam splitter configured to divide the primary illumination light beam into two partial illumination light beams. An illumination objective is configured to focus the partial illumination light beams onto and/or into a sample such that the partial illumination light beams extend, spatially separated from one another, through an entry pupil of the illumination objective and are spatially superposed on and/or in the sample after passing through the illumination objective. A phase influencer is configured to cause a relative phase offset of the partial illumination light beams with respect to one another in such a way that the partial illumination light beams in the entry pupil of the illumination objective have a phase offset of ?.
    Type: Application
    Filed: June 21, 2017
    Publication date: September 12, 2019
    Applicant: Leica Microsystems CMS GmbH
    Inventors: Florian FAHRBACH, Lars FRIEDRICH, Werner KNEBEL
  • Patent number: D862553
    Type: Grant
    Filed: June 28, 2018
    Date of Patent: October 8, 2019
    Assignee: Leica Microsystems CMS GmbH
    Inventor: Christophe Apothéloz
  • Patent number: D862554
    Type: Grant
    Filed: June 28, 2018
    Date of Patent: October 8, 2019
    Assignee: Leica Microsystems CMS GmbH
    Inventor: Christophe Apothéloz
  • Patent number: D862555
    Type: Grant
    Filed: June 28, 2018
    Date of Patent: October 8, 2019
    Assignee: Leica Microsystems CMS GmbH
    Inventor: Christophe Apothéloz
  • Patent number: D862556
    Type: Grant
    Filed: June 28, 2018
    Date of Patent: October 8, 2019
    Assignee: Leica Microsystems CMS GmbH
    Inventor: Christophe Apothéloz
  • Patent number: D862557
    Type: Grant
    Filed: June 28, 2018
    Date of Patent: October 8, 2019
    Assignee: Leica Microsystems CMS GmbH
    Inventor: Christophe Apothéloz
  • Patent number: D863391
    Type: Grant
    Filed: June 28, 2018
    Date of Patent: October 15, 2019
    Assignee: Leica Microsystems CMS GmbH
    Inventor: Christophe Apothéloz
  • Patent number: D876506
    Type: Grant
    Filed: October 6, 2017
    Date of Patent: February 25, 2020
    Assignee: Leica Microsystems CMS GmbH
    Inventor: Christophe Apothéloz
  • Patent number: D878444
    Type: Grant
    Filed: October 6, 2017
    Date of Patent: March 17, 2020
    Assignee: Leica Microsystems CMS GmbH
    Inventor: Christophe Apothéloz
  • Patent number: D924246
    Type: Grant
    Filed: September 20, 2018
    Date of Patent: July 6, 2021
    Assignee: Leica Microsystems CMS GmbH
    Inventor: Stefan Huber
  • Patent number: D924247
    Type: Grant
    Filed: September 20, 2018
    Date of Patent: July 6, 2021
    Assignee: Leica Microsystems CMS GmbH
    Inventor: Stefan Huber