Abstract: A computerized method of deep model matching for image transformation includes inputting pilot data and pre-trained deep model library into computer memories; performing a model matching scoring using the pilot data and the pre-trained deep model library to generate model matching score; and performing a model matching decision using the model matching score to generate a model matching decision output. Additional pilot data may be used to perform the model matching scoring and the model matching decision iteratively to obtain improved model matching decision output. Alternatively, the pre-trained deep model library may be pre-trained deep adversarial model library in the method.

Abstract: A method for illuminating a sample slice uses a light beam or a light sheet during single plane illumination microscopy (SPIM). The light beam or light sheet is deflected by an angle mirror having a first and second reflective surface reflecting a first and second portion of the light beam or light sheet, respectively, whereby the first and second portions of the light beam or light sheet spatially overlap one another after the deflecting. Alternatively, the light beam or light sheet is refracted by a refractive optical component comprising a first and second refractor surface refracting a first portion of the light beam or light sheet, respectively, whereby the first and second portions of the light beam or light sheet spatially overlap one another after the refracting.

Abstract: A method for imaging a sample using a microscope includes recording a first image of the sample, the first image being represented by image data. Sample information is extracted from the first image by analyzing the image data using an analyzer. At least a part of the sample is scanned with a light beam while modulating the light beam based on the extracted sample information. A second image of the sample is recorded during and/or after scanning the sample with the modulated light beam.

Abstract: A microscope system includes a light sheet microscopic functional unit that illuminates and images a sample in a first operating state with a light sheet-like illumination light distribution, and a scanning microscopic functional unit that illuminates and images the sample in a second operating state with a point-like illumination light distribution. A first scanning element uniaxially scans the sample, in the first operating state, with the light sheet-like illumination light distribution, and uniaxially scans the sample, in the second operating state, with the point-like illumination light distribution. A second scanning element uniaxially scans the sample, in the second operating state, with the point-like illumination light distribution, such that, in the second operating state, the second scanning element generates together with the first scanning element a biaxial scanning of the sample with the point-like illumination light distribution.

Abstract: A microscope includes an illumination system configured to illuminate a sample chamber with laser pulses. The illumination system includes a control device with stored, modifiable illumination parameters, with trigger outputs, to which at least one externally triggerable laser system is connectable in each case, and with a trigger generator configured to produce temporally successive trigger signals for triggering the at least one laser system. The microscope is configured such that an assignment of the trigger signals to the trigger outputs and/or a time interval between successive ones of the trigger signals depends on the illumination parameters.

Abstract: An illumination arrangement for a microscope includes an illumination input configured to inject an illumination beam bundle and an illumination output configured to output at least two partial beam bundles generated from the illumination beam bundle. At least one diffractive optical element is configured to split the illumination beam bundle into the at least two partial beam bundles that propagate along partial beam paths, and is configured to effect a relative change in respective propagation directions of the at least two partial beam bundles with respect to one another, such that the at least two partial beam bundles output by the illumination arrangement are non-collinear with respect to one another at the illumination output.

Abstract: A microscope system includes a light sheet microscopy functional unit and at least one further light microscopy functional unit. The light sheet microscopy functional unit has an illumination objective which is formed by a first objective and a detection objective which is formed by a separate second objective. The at least one further light microscopy functional unit has a detection objective that is formed by the second objective.

Abstract: A lightsheet microscope includes a detection objective for imaging a target region of a sample located in a focal plane, and an illumination objective for focusing an illumination light beam. The illumination objective and the detection objective define a sample chamber, in which a sample holder having a carrier surface is arranged. A light deflection device has a deflection surface arranged laterally offset in relation to the optical axis of the detection objective in the sample chamber which deflects the illumination light beam through the illumination objective in a direction perpendicular to the optical axis such that the deflected illumination light beam forms a lightsheet-type illumination light distribution. The light deflection device has a collision section facing toward the carrier surface. The carrier surface is inclined relative to the focal plane at a predetermined angle such that a part of the carrier surface is arranged in the focal plane.

Abstract: An optical device includes an optical system and a spectrally selective component arranged in a beam path and configured to spectrally influence light that propagates along the beam path. The spectrally selective component comprises an effective surface having a spectral edge that varies with the incidence site of the light on the effective surface. The effective surface of the spectrally selective component is arranged in the beam path at a point at which a variation of the spectral edge, which is caused by a variation of the incidence angle at which the light is incident onto the effective surface, is at least partly compensated for by an opposite-direction variation of the spectral edge which is caused by a variation of the incidence site. Alternatively, the effective surface is arranged in the beam path at the site of an image of a pupil of the optical system.

Abstract: The invention relates to a correction objective (10), comprising a first lens group (12) of positive optical power, a second lens group (14) of positive optical power, a third lens group (16) of negative optical power, and a fourth lens group (18) of positive optical power, which are arranged in this order from the object side, the second lens group (14) being movable along the optical axis (O) in such a way that the sum of the distance (V1) between the second lens group (14) and the first lens group (12) and the distance (V2) between the second lens group (14) and the third lens group (16) is constant. The image scale of the second lens group (14) lies in a range of ?0.9 to ?1.1.

Abstract: A method for evaluating a single-photon detector signal includes duplicating the single-photon detector signal into a first and a second signal. The first signal is processed and the second signal is either not processed or is processed in a manner different from the first signal. A differential signal is formed between the unprocessed or differently processed second signal and the processed first signal. The differential signal is evaluated to determine pulse events.

Abstract: A computerized domain matching image conversion method for transportable imaging applications first performs a target domain A to source domain B matching converter training by computing means using domain B training images and at least one domain A image to generate an A to B domain matching converter. The method then applies the A to B domain matching converter to a domain A application image to generate its domain B matched application image. The method further applies a domain B imaging application analytics to the domain B matched application image to generate an imaging application output for the domain A application image.

Abstract: An optical assembly includes a volume grating configured to influence a beam direction of at least one light beam, and a switching device arranged in a beam path upstream of the volume grating. The switching device is configured to switch the beam direction and/or beam position of the at least one light beam from a first beam direction and/or beam position, in which the at least one light beam does not impinge on the volume grating at an acceptance angle of the volume grating, to a second beam direction and/or beam position, in which the at least one light beam impinges on the volume grating at the acceptance angle, and/or vice versa.

Abstract: An image processing device for improving signal-to-noise of microscopy images includes a memory configured to store microscopy images at least temporarily, and processing circuitry configured to determine an output image based on a weighted rolling average of an ordered set of microscopy images stored in the memory. A method for improving signal-to-noise of microscopy images includes storing microscopy images at least temporarily, and determining an output image based on a weighted rolling average of an ordered set of the stored microscopy images.

Abstract: A feed device for an immersion medium for use with an objective enabling a specimen to be imaged microscopically includes a cap fitted releasably or fixedly to the objective and delimiting a receptacle space for the immersion medium. The cap has an exit opening aligned with an optical element of the objective facing the specimen. The immersion medium held in the receptacle space is feedable through the exit opening to a target space situated between the optical element of the objective and the specimen. A sensor is integrated in the cap and has an electrode structure configured to detect an amount of the immersion medium fed through the exit opening to the target space. The electrode structure at least partly encloses the exit opening and has a spatial detection region extending away from the exit opening in a radial direction.

Abstract: An electric circuit for driving an acousto-optic crystal includes a piezoelectric converter configured to drive the acousto-optic crystal to vibrate mechanically. A signaling cable is configured to conduct a first electrical alternating-current signal and a second electrical signal. The electric circuit further includes a first frequency-separating filter and a second frequency-separating filter, each of the frequency-separating filters having an input, a high-frequency output and a low-frequency output. The input of the first frequency-separating filter and the input of the second frequency-separating filter is connected to the signaling cable, and the high-frequency output of the second frequency-separating filter is connected to the piezoelectric converter.