Abstract: A method is useable for digitally correcting an optical image of a sample by a microscope that has a cover slip covering the sample. The method includes: determining, by the microscope, an index of refraction of an optical medium bordering the cover slip, a tilt of the cover slip, and/or a thickness of the cover slip; ascertaining an imaging error to be corrected in the form of a pupil function based on the index of refraction of the optical medium, the tilt of the cover slip, and/or the thickness of the cover slip; carrying out imaging of the sample by the microscope; and digitally correcting image data captured by the imaging of the sample based on the pupil function.

Abstract: A SPIM-microscope (Selective Plane Imaging Microscopy) and a method of operating the same having a y-direction illumination light source and a z-direction detection light camera. An x-scanner generates a sequential light sheet by scanning the illumination light beam in the x-direction. An electronic zoom is provided that is adapted to change the scanning length in the x-direction independently of a focal length of the illumination light beam and a size of the light sheet in the y-direction and in the z-direction, wherein the number of image pixels in x-direction is maintained unchanged by the electronic zoom independently of the scanning length in x-direction that has been selected.

Abstract: A computerized model compatibility regulation method for imaging applications first performs a target domain B application by computing means using at least one image X and target domain B image analytics to generate a target domain B application output for X. The method then applies a reference domain A application by computing means to generate reference domain A application output for X. The method further performs a compatibility assessment to generate at least one compatibility result for X. In addition, the method checks the compatibility result for X and if the check output is incompatible, the method performs online correction to generate a corrected application output for X.

Abstract: An oblique plane microscope includes a detection optical unit having an image sensor which has a sensor surface formed from sensor lines arranged in parallel, and a transport optical unit having an objective arranged for specimen illumination by a light sheet tilted relative to an optical axis of the transport optical unit and for imaging a specimen plane illuminated with the light sheet onto the sensor surface. An optical axis of the detection optical unit is tilted relative to the optical axis of the transport optical unit. The sensor lines each extend in an orthogonal direction with respect to the optical axis of the transport optical unit. The detection optical unit has an anamorphic magnification system. A magnification of an anamorphic magnification system of the detection optical unit, in a direction lying orthogonal to the sensor lines, is less than in a direction lying parallel to the sensor lines.

Abstract: A light sheet microscope includes a sample chamber in which a cover slip or slide is arrangeable, which has a surface that defines a partially reflective interface and which has a further surface that defines a further partially reflective interface. The two interfaces are arranged at different distances from an objective. The light sheet microscope further includes an optical system having the objective facing toward the cover slip or slide, an illumination apparatus, which is designed to generate a light sheet, a sensor, and a processor. The two interfaces are formed in that two optical media are applicable in the sample chamber. The light sheet microscope forms a measuring device for acquiring a measured variable. The sensor is designed to acquire the intensities and/or the incidence locations of the two reflection light beams.

Abstract: A computerized prediction guided learning method for classification of sequential data performs a prediction learning and a prediction guided learning by a computer program of a computerized machine learning tool. The prediction learning uses an input data sequence to generate an initial classifier. The prediction guided learning may be a semantic learning, an update learning, or an update and semantic learning. The prediction guided semantic learning uses the input data sequence, the initial classifier and semantic label data to generate an output classifier and a semantic classification. The prediction guided update learning uses the input data sequence, the initial classifier and label data to generate an output classifier and a data classification. The prediction guided update and semantic learning uses the input data sequence, the initial classifier and semantic and label data to generate an output classifier, a semantic classification and a data classification.

Abstract: Systems and methods for processing biological specimens are provided. The biological specimen processing system generally includes a flow cell carrier for holding a microfluidic flow cell and a fluidic handling unit attachable to the flow cell carrier. The fluidic handling unit interfaces with the microfluidic flow cell and can include fluidic pumps, fluidic connections, integrated electronics, and processing software to facilitate processing of a biological specimen contained in the microfluidic flow cell.

Abstract: A light sheet microscope includes an illuminator having a beam source which is designed to direct an illumination beam propagating along an illumination axis onto a sample. A light-sheet generator is designed to generate a light-sheet-like illumination light distribution illuminating the sample in a partial area from the illumination beam. A detection unit has a detector which is designed to capture detection light originating from the partial area of the sample illuminated with the illumination light distribution. The illuminator comprises a beam modulator adapted to modulate the illumination beam along the illumination axis in such a way that light exposure of the partial area of the sample illuminated by the illumination light distribution varies along the illumination axis.

Abstract: A microscope includes an optical imaging system with an adjustable corrector, a microscope drive, a position sensitive detector, an optical measuring system and a control unit. The optical measuring system configured to form first and second measuring light beams, direct the measuring light beams into an entrance pupil of the optical imaging system eccentrically with first and second distances to the optical axis thereof, receive first and second reflection light beams, and direct the reflection light beams onto the position sensitive detector. The control unit is configured to record positions of the reflection light beams on the position sensitive detector, and determine an aberration based on the recorded positions.

Abstract: The invention discloses a method for reduction of autofluorescence in biological samples, comprising the steps of: a) providing a biological microscopy sample; b) irradiating the sample with visible light, wherein the visible light has a spectrum such that at least 50% of the light intensity emanates from a narrow wavelength interval within the visible range. The invention also discloses a method for autofluorescence reduction with triplet sensitizers irradiated with visible light.

Abstract: A collection device for collecting a sample dissected or ablated from an object includes a collection container configured to collect the dissected or ablated sample. An electrode pair is arranged on a side of a base of the collection container facing away from the object or on or in the base itself. The electrodes of the electrode pair are arranged concentrically. A voltage supply is configured to apply a voltage to the electrodes of the electrode pair such that a divergent electric field is generated at least in a region of the collection container.

Abstract: A changing system for a microscope includes multiple afocal enlargement changing modules of different enlargement levels that are optionally introducible into an infinite beam path running along an optical axis of the microscope. Each of the enlargement changing modules contain a light deflection system. The light deflection systems are designed to adjust the path length of the infinite beam path passing through the respective enlargement changing module in such a way that all of the enlargement changing modules, regardless of the different enlargement levels of the enlargement changing modules, map an exit pupil of a lens of the microscope onto the same location along the optical axis.

Abstract: A computerized method of deep model matching for image transformation includes inputting pilot data and pre-trained deep model library into computer memories; performing a model matching scoring using the pilot data and the pre-trained deep model library to generate model matching score; and performing a model matching decision using the model matching score to generate a model matching decision output. Additional pilot data may be used to perform the model matching scoring and the model matching decision iteratively to obtain improved model matching decision output. Alternatively, the pre-trained deep model library may be pre-trained deep adversarial model library in the method.

Abstract: A method for illuminating a sample slice uses a light beam or a light sheet during single plane illumination microscopy (SPIM). The light beam or light sheet is deflected by an angle mirror having a first and second reflective surface reflecting a first and second portion of the light beam or light sheet, respectively, whereby the first and second portions of the light beam or light sheet spatially overlap one another after the deflecting. Alternatively, the light beam or light sheet is refracted by a refractive optical component comprising a first and second refractor surface refracting a first portion of the light beam or light sheet, respectively, whereby the first and second portions of the light beam or light sheet spatially overlap one another after the refracting.

Abstract: A method for imaging a sample using a microscope includes recording a first image of the sample, the first image being represented by image data. Sample information is extracted from the first image by analyzing the image data using an analyzer. At least a part of the sample is scanned with a light beam while modulating the light beam based on the extracted sample information. A second image of the sample is recorded during and/or after scanning the sample with the modulated light beam.

Abstract: A microscope system includes a light sheet microscopic functional unit that illuminates and images a sample in a first operating state with a light sheet-like illumination light distribution, and a scanning microscopic functional unit that illuminates and images the sample in a second operating state with a point-like illumination light distribution. A first scanning element uniaxially scans the sample, in the first operating state, with the light sheet-like illumination light distribution, and uniaxially scans the sample, in the second operating state, with the point-like illumination light distribution. A second scanning element uniaxially scans the sample, in the second operating state, with the point-like illumination light distribution, such that, in the second operating state, the second scanning element generates together with the first scanning element a biaxial scanning of the sample with the point-like illumination light distribution.

Abstract: A microscope system includes a light sheet microscopy functional unit and at least one further light microscopy functional unit. The light sheet microscopy functional unit has an illumination objective which is formed by a first objective and a detection objective which is formed by a separate second objective. The at least one further light microscopy functional unit has a detection objective that is formed by the second objective.

Abstract: An illumination arrangement for a microscope includes an illumination input configured to inject an illumination beam bundle and an illumination output configured to output at least two partial beam bundles generated from the illumination beam bundle. At least one diffractive optical element is configured to split the illumination beam bundle into the at least two partial beam bundles that propagate along partial beam paths, and is configured to effect a relative change in respective propagation directions of the at least two partial beam bundles with respect to one another, such that the at least two partial beam bundles output by the illumination arrangement are non-collinear with respect to one another at the illumination output.

Abstract: A microscope includes an illumination system configured to illuminate a sample chamber with laser pulses. The illumination system includes a control device with stored, modifiable illumination parameters, with trigger outputs, to which at least one externally triggerable laser system is connectable in each case, and with a trigger generator configured to produce temporally successive trigger signals for triggering the at least one laser system. The microscope is configured such that an assignment of the trigger signals to the trigger outputs and/or a time interval between successive ones of the trigger signals depends on the illumination parameters.