Abstract: The invention relates to a method of preparing and using a library of template polynucleotides suitable for use as templates in solid-phase nucleic acid amplification and sequencing reactions to determine the methylation status of the cytosine bases in the library. In particular, the invention relates to a method of preparing and analysing a library of template polynucleotides suitable for methylation analysis.

Abstract: A carbon nanotube field effect transistor (CNFET), that has a channel formed of carbon nanotubes (CNTs), includes a layered deposit of a nonstoichiometric doping oxide (NDO), such as HfOX, where the concentration of the NDO varies through the thickness of the layer(s). An n-type metal-oxide semiconductor (NMOS) CNFET made in this manner can achieve similar ON-current, OFF-current, and/or threshold voltage magnitudes to a corresponding p-type metal-oxide semiconductor (PMOS) CNFET. Such an NMOS and PMOS can be used to achieve a symmetric complementary metal-oxide semiconductor (CMOS) CNFET design.

Abstract: A learning device calculates a feature of each data included in a pair of datasets in which two modalities among a plurality of modalities are combined, using a model that receives data on a corresponding modality among the modalities and outputs a feature obtained by mapping the received data into an embedding space. The learning device then selects similar data similar to each target data that is data on a first modality in a first dataset of the datasets, from data on a second modality included in a second dataset of the datasets. The learning device further updates a parameter of the model such that the features of the data in the pair included in the first and the second datasets are similar to one another, and the feature of data paired with the target data is similar to the feature of data paired with the similar data.

Abstract: In coherent beam combining, the beams can be phase-modulated with a pseudo-random bit sequence (PRBS) to prevent stimulated Brillouin scattering (SBS) downstream. To coherently combine the phase-modulated beams, however, the PRBS waveforms should be true-time-delayed to within a small fraction of the bit duration. Traditionally, this true time delay is achieved by cutting optical fibers to length or with optical trombones. But trimming fibers is hard to do precisely, and optical trombones have large insertion loss. In addition, the path length mismatch varies as the fibers heat up and/or vibrate. Here, the beams are generated from a kilohertz linewidth seed split among N>1 (e.g., N=100) arms. Each arm is phase-modulated with a separate copy of the unique PRBS pattern. The relative phase of the PRBS patterns is stabilized by phase-locking the master oscillators used to read out the PRBS patterns.

Abstract: Materials (e.g., particles, hydrogels) that provide extended release of one or more therapeutic agents are useful platforms for drug delivery. In part, the present invention relates to new triblock (ABC) bottlebrush copolymers which can be used in the formulation of particles and hydrogels for the extended release of therapeutic agents. In certain embodiments, the triblock bottlebrush copolymers, particles, and hydrogels described herein are thermally-responsive and gel at physiological temperature (e.g., upon administration to a subject), providing injectable and/or implantable gels which can be used for extended release drug delivery. The present invention also provides methods for extended release drug delivery, and methods of treating and/or preventing a disease or conditions in a subject, using the inventive copolymers, particles, and hydrogels. In addition, the present invention provides methods of preparing the triblock bottlebrush copolymers described herein.

Abstract: Space-based ground-imaging lidar has become increasingly feasible with recent advances in compact fiber lasers and single-photon-sensitive Geiger-mode detector arrays. A challenge with such a system is imperfect pointing knowledge caused by angular jitter, exacerbated by long distances between the satellite and the ground. Unless mitigated, angular jitter blurs the 3D lidar data. Unfortunately, using mechanical isolation, advanced IMUs, star trackers, or auxiliary passive optical sensors to reduce the error in pointing knowledge increases size, weight, power, and/or cost. Here, the 2-axis jitter time series is estimated from the lidar data. Simultaneously, a single-surface model of the ground is estimated as nuisance parameters. Expectation Maximization separates signal and background detections, while maximizing the joint posterior probability density of the jitter and surface states.

Abstract: This disclosure provides systems, methods, and compositions for site-specific genetic engineering using Programmable Addition via Site-Specific Targeting Elements (PASTE). PASTE comprises the addition of an integration site into a target genome followed by the insertion of one or more genes of interest or one or more nucleic acid sequences of interest at the site. PASTE combines gene editing technologies and integrase technologies to achieve unidirectional incorporation of genes in a genome for the treatment of diseases and diagnosis of disease.

Abstract: Control of volume in bioreactors and associated systems is generally described. Feeding and/or sampling strategies can be employed, in some embodiments, such that the working volume within the bioreactor remains substantially constant.

Abstract: Compositions, devices, and methods relating to the use of mixed-matrix membranes containing metal-organic frameworks to separate gases are generally described. In some embodiments, branched nanoparticles made at least in part of metal-organic frameworks are described. In some embodiments, the morphology and size of the branched nanoparticles are controlled by the presence of a chemical modulator during synthesis. In some embodiments, the branched nanoparticles are uniformly distributed in a mixed-matrix membrane. In some embodiments, the mixed-matrix membrane is configured to separate one or more gases from a gas mixture. In some embodiments, the branched nanoparticles contribute at least in part to an increase in permeability, selectivity, and/or resistance to plasticization of the mixed-matrix membrane.

Abstract: A primary resonant coil (22) and a secondary resonant coil (23) are different in the proposed improved coreless power transformer design, wherein the differences between the two resonant coils are achieved in two ways: (1) in the first difference, the two resonant coil windings are made to be different; for example, to have different number of turns, turn spacing and/or different wire sizes, and (2) the second difference is with the capacitance values of the two parallel capacitors each used to resonate their respective coils.

Abstract: Embodiments disclosed herein relate to an ingestible electronic device configured to provide short term electrical stimulation to a stomach of a subject. The device may include a non-expandable housing and two or more electrodes disposed on the non-expandable housing to apply the short term electrical stimulation to the stomach of the subject. In some instances, the short term electrical stimulation may be on the order of 1 hour or less. In some instances, the ingestible electronic device may utilize the natural gastrointestinal processes of the subject to retain the ingestible electronic device for an appropriate time period within the stomach. For example, the subject may first ingest the ingestible electronic device and the device may travel into the stomach of the subject. The device may then apply electrical stimulation to the stomach for an appropriate time period prior to passing out of the stomach via a pyloric orifice of the stomach.

Abstract: A contactless sensor includes a cardiac beamformer, a wireless-to- seismocardiogram translator, and an automatic labeler. The cardiac beamformer determines at least one beam for receiving wireless signals generated based on movement of a heart. The at least one beam is generated based on phase information and a heart signal extracted from a time-domain signal generated from one or more receiver elements. The wireless-to-seismocardiogram translator implements a convolutional neural network to transform time-series data detected from the at least one beam to a seismocardiogram. The automatic labeler identifies and labels one or more micro-cardiac events in the time-series data. The cardiac beamformer may be considered an optional feature in one or more implementations.

Abstract: Described is a flexible thermally-drawn receive phased array antenna and antenna system and a process for making the same. The phased array antenna system includes a plurality of antenna elements and one or more low noise amplifiers (LNAs) encapsulated in a fiber such as flexible polyetherimide (PEI) fiber material. A DC bias for the LNAs and other components is provided by one or more electrically conducting wires disposed in proximity to the antenna elements and also encapsulated in the fiber. In embodiments, the antenna elements are provided as dipole antenna elements and the phased array antenna is provided as a flexible thermally-drawn linear dipole receive phased array antenna operable at UHF frequencies.

Abstract: The invention provides for delivery, engineering and optimization of systems, methods, and compositions for manipulation of sequences and/or activities of target sequences. Provided are vectors and vector systems, some of which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors. Also provided are methods of directing CRISPR complex formation in prokaryotic and eukaryotic cells to ensure enhanced specificity for target recognition and avoidance of toxicity.

Abstract: The present disclosure relates generally to technologies comprising engineered immune cells that express chimeric antigen receptors (CARs) that specifically bind to a membrane-inserting amphiphilic ligand. Also disclosed herein are methods and compositions for treating a tumor.

Abstract: The invention provides for systems, methods, and compositions for manipulation of sequences and/or activities of target sequences. Provided are vectors and vector systems, some of which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors. Also provided are methods of directing CRISPR complex formation in eukaryotic cells and methods for selecting specific cells by introducing precise mutations utilizing the CRISPR/Cas system.

Abstract: A high density wiring system to transmit radio frequency (RF) signals to superconducting qubits disposed within a dilution refrigerator is disclosed. The high density wiring system comprises a plurality of flexible layers, with conductive layers disposed between adjacent pairs of flexible layers. The conductive layers may be constructed using phosphor bronze. The wiring system that is disposed at the lower temperature stages of the dilution refrigerator may be coated with a superconducting material. The wiring system is useful for superconducting quantum computers and cryogenic sensors, as well as cryogenic infrastructure.

Abstract: Resistive switching devices that contain lithium, including resistive switching devices containing a lithium titanate, and associated systems and methods are generally described. In some cases, the resistive switching device contains a lithium titanate-containing domain, a first electrode, and a second electrode. In some cases, the application of an electrical potential to the resistive switching device causes a change in resistance state of the lithium titanate-containing domain. The resistive switching devices described herein may be useful as memristors, and in applications that include Resistive-random access memory and neuromorphic computing.

Abstract: Articles and methods for processing aluminum are generally described. The aluminum can include compositions of gallium and/or indium such that the aluminum is activated to react with water.

Abstract: A method and system for medical imaging employs an excitation source configured to cause an object having a plurality of cells to at least one of emit, reflect, and fluoresce light. An optical receptor is employed that is configured to receive the light from the object. A filter assembly receives the light from the optical receptor and filters the light. An image processor having a field of view (FOV) substantially greater than a diameter of a cell of the object and an analysis resolution substantially matched to the diameter of a cell of the object that receives the filtered light from the filter and analyzes the filtered light corresponding to each cell in the FOV. A feedback system is provided that is configured to provide an indication of a state of each cell in the FOV and a location of a cell in the FOV meeting a predetermined condition.