Abstract: A nickel-based alloy composition consisting, in weight percent, of: between 4.0% and 6.9% aluminium, between 0.0% and 23.4% cobalt, between 9.1% and 11.9% chromium, between 0.1% and 4.0% molybdenum, between 0.6% and 3.7% niobium, between 0.0 and 1.0% tantalum, between 0.0% and 3.0% titanium, between 0.0% and 10.9% tungsten, between 0.02 wt. % and 0.35 wt. % carbon, between 0.001 and 0.2 wt. % boron, between 0.001 wt. % and 0.5 wt. %. zirconium, between 0.0 and 0.5% silicon, between 0.0 and 0.1% yttrium, between 0.0 and 0.1% lanthanum, between 0.0 and 0.1% cerium, between 0.0 and 0.003% sulphur, between 0.0 and 0.25% manganese, between 0.0 and 0.5% copper, between 0.0 and 0.5% hafnium, between 0.0 and 0.5% vanadium, between 0.0 and 10.0% iron, the balance being nickel and incidental impurities.

Abstract: Recombinant adenoviral vectors, immunogenic compositions thereof and their use in medicine, and methods for generating recombinant adenoviral vectors are provided. In particular, the an adenovirus vector having a capsid derived from chimpanzee adenovirus AdY25, wherein the capsid encapsidates a nucleic acid molecule comprising an exogeneous nucleotide sequence of interest are provided.

Abstract: The present application discloses a measurement device incorporating a modular microfluidic system comprising standard modules which can be networked together via standardised connectors in a range of configurations. The measurement device includes a master microfluidic module connectable to an external pressure source, which has a socket for receiving a reagent cartridge, and can be used to drive flow of reagent from the reagent cartridge to an analysis chip. The master microfluidic module also has specialised pressure output and reagent input connectors which can be attached to secondary microfluidic modules, so that the master microfluidic modules can drive reagent from the secondary microfluidic modules to an analysis chip supported on the main microfluidic module. The application also describes a range of specialised cartridge types for use with the measurement device.

Abstract: The invention relates to mutant forms of lysenin. The invention also relates to analyte characterisation using lysenin.

Abstract: Provided herein relate to modified or mutant forms of secretin and compositions comprising the same. In particular, the modified or mutant forms of secretin permits efficient capture and/or translocation of an analyte through the modified or mutant secretin nanopores. Methods for using unmodified secretin or the modified or mutant forms of secretin and compositions, for example, for characterizing an analyte, e.g., a target polynucleotide, are also provided.

Abstract: A process for analysing chromosome regions and interactions relating to immunoresponsiveness.

Abstract: The invention relates to a new method of determining in a sample the presence or absence of one or more analyte members of a group of two or more analytes. The invention therefore relates to a multiplex assay for determining the presence or absence of each analyte in a group of multiple analytes. The assay uses aptamers and transmembrane pores.

Abstract: There is provided a method of reducing noise in a cryogenic cooling system associated with a mechanical refrigerator forming part of said cooling system. The method comprises: monitoring vibrations in the cooling system during operation of the mechanical refrigerator; and modulating an operating frequency of the mechanical refrigerator based on the monitored vibrations so as to reduce the amplitude of said vibrations. This allows noise within the cooling system to be reduced.

Abstract: A method of determining the presence of bacteria expressing cytochrome c oxidase (‘the bacteria’), the method comprising: —providing a sample suspected of containing the bacteria; —providing a compound that has two redox states: a reduced state and an oxidised state, wherein cytochrome c oxidase can convert the compound from its reduced state to its oxidised state; —contacting an electrode either with (i) the compound in its oxidised state in the presence of the sample, then applying a reductive potential and measuring the current at the electrode; or (ii) the compound in its reduced state in the presence of the sample, then applying an oxidative potential and measuring the current at the electrode; and—comparing the magnitude of the current produced by the reductive potential or oxidative potential in the presence of the sample suspected of containing the bacteria with the magnitude of the current produced under the same conditions, but in the absence of the sample suspected of containing the bacteria, where

Abstract: Provided are an artificial multi-antigen fusion protein and a preparation method thereof. The fusion protein can effectively stimulate CD8+T and CD4+ T cell immunities, and can be applied to immunodiagnostics or serve as a prophylactic or therapeutic vaccine.

Abstract: The invention relates to a new method of determining the presence, absence or one or more characteristics of multiple analytes. The invention concerns coupling a first analyte to a membrane containing a detector and investigating the first analyte using the detector. The invention also concerns coupling a second analyte to the membrane and investigating the second analyte. The first analyte is uncoupled form the membrane prior to investigating the second analyte. The invention also relates to polynucleotide sequencing.

Abstract: The invention relates to a method for modifying a template double stranded polynucleotide, especially for characterisation using nanopore sequencing. The method produces from the template a plurality of modified double stranded polynucleotides. These modified polynucleotides can then be characterised.

Abstract: The present invention relates to a method for preparing a stabilised crystalline A/M/X material comprising an oxide of formula [Z]pOq and a compound of formula [A]a[M]b[X]c, wherein [Z] comprises at least one element Z capable of forming an oxide with a band gap of at least 3 eV; p and q are positive numbers; [A] comprises one or more A cations; [M] comprises one or more M cations; [X] comprises one or more X anions; a is an integer from 1 to 6; b is an integer from 1 to 6; and c is an integer from 1 to 18. Often, the stabilised crystalline A/M/X material is a perovskite. The invention also provides a stabilised crystalline A/M/X material, which can be produced by the process of the invention. The invention further provides materials and devices containing the stabilised crystalline A/M/X material of the invention.

Abstract: The present invention relates to a semiconductor device comprising a semiconducting material, wherein the semiconducting material comprises a compound comprising: (i) one or more first monocations [A]; (ii) one or more second monocations [BI]; (iii) one or more trications [BIII]; and (iv) one or more halide anions [X]. The invention also relates to a process for producing a semiconductor device comprising said semiconducting material. Also described is a compound comprising: (i) one or more first monocations [A]; (ii) one or more second monocations [BI] selected from Cu+, Ag+ and Au+; (iii) one or more trications [BIII]; and (iv) one or more halide anions [X].

Abstract: The invention relates to a new method of delivering an analyte to a transmembrane pore in a membrane. The method involves the use of microparticles.

Abstract: An interferometric scattering microscope is adapted by performing spatial filtering of output light, which comprises both light scattered from a sample location and illuminating light reflected from the sample location, prior to detection of the output light. The spatial filtering passes the reflected illumination light but with a reduction in intensity that is greater within a predetermined numerical aperture than at larger numerical apertures. This enhances the imaging contrast for coherent illumination, particularly for objects that are weak scatterers.

Abstract: Disclosed preamplifier circuit comprises amplifier arranged in first current path between input node and output node of the preamplifier circuit. Feedback capacitor is arranged in second current path between said input and output nodes. Feedback circuit having adjustable transfer function is arranged in third current path between said input and output nodes. Reset switch arranged in said third current path enables selectively coupling output of the feedback circuit to input of the amplifier and decoupling output of the feedback circuit from input of the amplifier. Loop controller is arranged selectively, in dependence of voltage in the preamplifier circuit, one of open reset switch to set preamplifier circuit in normal operating mode and close reset switch to set preamplifier circuit in reset mode. Loop controller is arranged to adjust the transfer function of the feedback circuit at least in part in dependence of the current operating mode of the preamplifier circuit.

Abstract: A nanopore sensing device comprises a planar structure provided with plural fluidic passages extending between the first and second chambers. The planar structure supports nanopores in membranes across respective passages and sensor electrodes are arranged to sense a fluidic electrical potential in respective passages between the nanopores and the second chamber. The passages comprise planar fluidic resistor portions between the sensor electrode and the second chamber, the planar fluidic resistor portions extending in a planar direction of the planar structure and being configured to form a fluidic resistor.

Abstract: The invention provides a method of investigating the spatial organisation of proteins in or on cells, and the use of that spatial organisation information to inform decisions about medical interventions, especially in relation to cancer treatments including CAR-T therapy. The method involves detecting one or more species of proteins on each of the plurality of cells; obtaining respective spatial coordinates of the detected proteins within the plurality of cells; detecting boundaries of the plurality of cells; and constructing a data vector based on the obtained spatial coordinates and the detected boundaries.

Abstract: The present invention pertains generally to the field of therapeutic compounds. More specifically the present invention pertains to certain 2-amino-N-(arylsulfinyl)-acetamide compounds that, inter alia, inhibit (e.g., selectively inhibit) bacterial aminoacyl-tRNA synthetase (aaRS) (e.g., bacterial leucyl-t RNA synthetase, LeuRS). The present invention also pertains to pharmaceutical compositions comprising such compounds, and the use of such compounds and compositions, both in vitro and in vivo, to inhibit (e.g., selectively inhibit) bacterial aminoacyl-tRNA synthetase; to treat disorders that are ameliorated by the inhibition (e.g., selective inhibition) of bacterial aminoacyl-tRNA synthetase; to treat bacterial infections; etc.