Patents Assigned to Prognosys Biosciences, Inc.
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Publication number: 20240084366Abstract: The present invention provides assays and assay systems for use in spatially encoded biological assays. The invention provides an assay system comprising an assay capable of high levels of multiplexing where reagents are provided to a biological sample in defined spatial patterns; instrumentation capable of controlled delivery of reagents according to the spatial patterns; and a decoding scheme providing a readout that is digital in nature.Type: ApplicationFiled: November 21, 2023Publication date: March 14, 2024Applicant: Prognosys Biosciences, Inc.Inventor: Mark S. Chee
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Patent number: 11913952Abstract: Provided herein are compositions and methods for detecting the binding of a peptide to an MHC molecule, and the binding of a peptide:MHC complex to a TCR. In preferred embodiments, the compositions and methods are in a highly-multiplexed way. The compositions and methods disclosed herein can be used to provide direct information on which peptides are bound to an MHC molecule. Also provided is a method for simultaneously detecting a large number of peptides for binding to an MHC molecule and/or a T cell. A method for detecting competitive binding of a large number of peptides to an MHC molecule and/or a T cell is also disclosed. Also provided herein is a method for simultaneously detecting a large number of specific TCRs. The compositions and methods of the present invention are useful for vaccine design, research and monitoring of autoimmune and infectious disease, immunogenicity testing of therapeutics, and tissue typing.Type: GrantFiled: November 22, 2021Date of Patent: February 27, 2024Assignee: Prognosys Biosciences, Inc.Inventors: John Andrew Altin, Mark S. Chee
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Publication number: 20240060115Abstract: The present disclosure provides methods and assay systems for use in spatially encoded biological assays, including assays to determine a spatial pattern of abundance, expression, and/or activity of one or more biological targets across multiple sites in a sample. In particular, the biological targets comprise proteins, and the methods and assay systems do not depend on imaging techniques for the spatial information of the targets. The present disclosure provides methods and assay systems capable of high levels of multiplexing where reagents are provided to a biological sample in order to address tag the sites to which reagents are delivered; instrumentation capable of controlled delivery of reagents; and a decoding scheme providing a readout that is digital in nature.Type: ApplicationFiled: November 3, 2023Publication date: February 22, 2024Applicant: Prognosys Biosciences, Inc.Inventors: Mark S. Chee, David A. Routenberg
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Patent number: 11866770Abstract: The present invention provides assays and assay systems for use in spatially encoded biological assays. The invention provides an assay system comprising an assay capable of high levels of multiplexing where reagents are provided to a biological sample in defined spatial patterns; instrumentation capable of controlled delivery of reagents according to the spatial patterns; and a decoding scheme providing a readout that is digital in nature.Type: GrantFiled: May 26, 2023Date of Patent: January 9, 2024Assignee: Prognosys Biosciences, Inc.Inventor: Mark S. Chee
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Patent number: 11821024Abstract: The present disclosure provides methods and assay systems for use in spatially encoded biological assays, including assays to determine a spatial pattern of abundance, expression, and/or activity of one or more biological targets across multiple sites in a sample. In particular, the biological targets comprise proteins, and the methods and assay systems do not depend on imaging techniques for the spatial information of the targets. The present disclosure provides methods and assay systems capable of high levels of multiplexing where reagents are provided to a biological sample in order to address tag the sites to which reagents are delivered; instrumentation capable of controlled delivery of reagents; and a decoding scheme providing a readout that is digital in nature.Type: GrantFiled: March 31, 2023Date of Patent: November 21, 2023Assignee: Prognosys Biosciences, Inc.Inventors: Mark S. Chee, David A. Routenberg
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Publication number: 20230332211Abstract: The present invention provides assays and assay systems for use in spatially encoded biological assays. The invention provides an assay system comprising an assay capable of high levels of multiplexing where reagents are provided to a biological sample in defined spatial patterns; instrumentation capable of controlled delivery of reagents according to the spatial patterns; and a decoding scheme providing a readout that is digital in nature.Type: ApplicationFiled: January 23, 2023Publication date: October 19, 2023Applicant: Prognosys Biosciences, Inc.Inventor: Mark S. Chee
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Publication number: 20230323436Abstract: The present invention provides assays and assay systems for use in spatially encoded biological assays. The invention provides an assay system comprising an assay capable of high levels of multiplexing where reagents are provided to a biological sample in defined spatial patterns; instrumentation capable of controlled delivery of reagents according to the spatial patterns; and a decoding scheme providing a readout that is digital in nature.Type: ApplicationFiled: May 26, 2023Publication date: October 12, 2023Applicant: Prognosys Biosciences, Inc.Inventor: Mark S. Chee
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Publication number: 20230304074Abstract: The present disclosure provides methods and assay systems for use in spatially encoded biological assays, including assays to determine a spatial pattern of abundance, expression, and/or activity of one or more biological targets across multiple sites in a sample. In particular, the biological targets comprise proteins, and the methods and assay systems do not depend on imaging techniques for the spatial information of the targets. The present disclosure provides methods and assay systems capable of high levels of multiplexing where reagents are provided to a biological sample in order to address tag the sites to which reagents are delivered; instrumentation capable of controlled delivery of reagents; and a decoding scheme providing a readout that is digital in nature.Type: ApplicationFiled: March 31, 2023Publication date: September 28, 2023Applicant: Prognosys Biosciences, Inc.Inventors: Mark S. Chee, David A. Routenberg
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Patent number: 11767550Abstract: The present invention provides assays and assay systems for use in spatially encoded biological assays. The invention provides an assay system comprising an assay capable of high levels of multiplexing where reagents are provided to a biological sample in defined spatial patterns; instrumentation capable of controlled delivery of reagents according to the spatial patterns; and a decoding scheme providing a readout that is digital in nature.Type: GrantFiled: January 9, 2023Date of Patent: September 26, 2023Assignee: Prognosys Biosciences, Inc.Inventor: Mark S. Chee
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Patent number: 11761030Abstract: The present invention provides assays and assay systems for use in spatially encoded biological assays. The invention provides an assay system comprising an assay capable of high levels of multiplexing where reagents are provided to a biological sample in defined spatial patterns; instrumentation capable of controlled delivery of reagents according to the spatial patterns; and a decoding scheme providing a readout that is digital in nature.Type: GrantFiled: December 5, 2022Date of Patent: September 19, 2023Assignee: Prognosys Biosciences, Inc.Inventor: Mark S. Chee
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Patent number: 11753674Abstract: The present disclosure provides methods and assay systems for use in spatially encoded biological assays, including assays to determine a spatial pattern of abundance, expression, and/or activity of one or more biological targets across multiple sites in a sample. In particular, the biological targets comprise proteins, and the methods and assay systems do not depend on imaging techniques for the spatial information of the targets. The present disclosure provides methods and assay systems capable of high levels of multiplexing where reagents are provided to a biological sample in order to address tag the sites to which reagents are delivered; instrumentation capable of controlled delivery of reagents; and a decoding scheme providing a readout that is digital in nature.Type: GrantFiled: July 31, 2020Date of Patent: September 12, 2023Assignee: Prognosys Biosciences, Inc.Inventors: Mark S. Chee, David A. Routenberg
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Patent number: 11732292Abstract: The present invention provides assays and assay systems for use in spatially encoded biological assays. The invention provides an assay system comprising an assay capable of high levels of multiplexing where reagents are provided to a biological sample in defined spatial patterns; instrumentation capable of controlled delivery of reagents according to the spatial patterns; and a decoding scheme providing a readout that is digital in nature.Type: GrantFiled: October 19, 2022Date of Patent: August 22, 2023Assignee: Prognosys Biosciences, Inc.Inventor: Mark S. Chee
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Patent number: 11733238Abstract: The present invention provides assays and assay systems for use in spatially encoded biological assays. The invention provides an assay system comprising an assay capable of high levels of multiplexing where reagents are provided to a biological sample in defined spatial patterns; instrumentation capable of controlled delivery of reagents according to the spatial patterns; and a decoding scheme providing a readout that is digital in nature.Type: GrantFiled: June 26, 2020Date of Patent: August 22, 2023Assignee: Prognosys Biosciences, Inc.Inventor: Mark S. Chee
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Publication number: 20230212655Abstract: The present invention provides assays and assay systems for use in spatially encoded biological assays. The invention provides an assay system comprising an assay capable of high levels of multiplexing where reagents are provided to a biological sample in defined spatial patterns; instrumentation capable of controlled delivery of reagents according to the spatial patterns; and a decoding scheme providing a readout that is digital in nature.Type: ApplicationFiled: March 14, 2023Publication date: July 6, 2023Applicant: Prognosys Biosciences, Inc.Inventor: Mark S. Chee
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Publication number: 20230175057Abstract: The present invention provides oligonucleotide constructs, sets of such oligonucleotide constructs, and methods of using such oligonucleotide constructs to provide validated sequences or sets of validated sequences corresponding to desired ROIs. Such validated ROIs and constructs containing these have a wide variety of uses, including in synthetic biology, quantitative nucleic acid analysis, polymorphism and/or mutation screening, and the like.Type: ApplicationFiled: October 12, 2022Publication date: June 8, 2023Applicant: Prognosys Biosciences, Inc.Inventor: Mark S. CHEE
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Patent number: 11634756Abstract: The present invention provides assays and assay systems for use in spatially encoded biological assays. The invention provides an assay system comprising an assay capable of high levels of multiplexing where reagents are provided to a biological sample in defined spatial patterns; instrumentation capable of controlled delivery of reagents according to the spatial patterns; and a decoding scheme providing a readout that is digital in nature.Type: GrantFiled: October 24, 2022Date of Patent: April 25, 2023Assignee: Prognosys Biosciences, Inc.Inventor: Mark S. Chee
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Patent number: 11618918Abstract: The present disclosure provides methods and assay systems for use in spatially encoded biological assays, including assays to determine a spatial pattern of abundance, expression, and/or activity of one or more biological targets across multiple sites in a sample. In particular, the biological targets comprise proteins, and the methods and assay systems do not depend on imaging techniques for the spatial information of the targets. The present disclosure provides methods and assay systems capable of high levels of multiplexing where reagents are provided to a biological sample in order to address tag the sites to which reagents are delivered; instrumentation capable of controlled delivery of reagents; and a decoding scheme providing a readout that is digital in nature.Type: GrantFiled: June 2, 2022Date of Patent: April 4, 2023Assignee: Prognosys Biosciences, Inc.Inventors: Mark S. Chee, David A. Routenberg
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Publication number: 20230042088Abstract: The present invention provides assays and assay systems for use in spatially encoded biological assays. The invention provides an assay system comprising an assay capable of high levels of multiplexing where reagents are provided to a biological sample in defined spatial patterns; instrumentation capable of controlled delivery of reagents according to the spatial patterns; and a decoding scheme providing a readout that is digital in nature.Type: ApplicationFiled: October 24, 2022Publication date: February 9, 2023Applicant: Prognosys Biosciences, Inc.Inventor: Mark S. Chee
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Patent number: 11560587Abstract: The present invention provides assays and assay systems for use in spatially encoded biological assays. The invention provides an assay system comprising an assay capable of high levels of multiplexing where reagents are provided to a biological sample in defined spatial patterns; instrumentation capable of controlled delivery of reagents according to the spatial patterns; and a decoding scheme providing a readout that is digital in nature.Type: GrantFiled: August 1, 2022Date of Patent: January 24, 2023Assignee: Prognosys Biosciences, Inc.Inventor: Mark S. Chee
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Patent number: 11549138Abstract: The present invention provides assays and assay systems for use in spatially encoded biological assays. The invention provides an assay system comprising an assay capable of high levels of multiplexing where reagents are provided to a biological sample in defined spatial patterns; instrumentation capable of controlled delivery of reagents according to the spatial patterns; and a decoding scheme providing a readout that is digital in nature.Type: GrantFiled: May 26, 2022Date of Patent: January 10, 2023Assignee: Prognosys Biosciences, Inc.Inventor: Mark S. Chee