Patents Assigned to Prognosys Biosciences, Inc.
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Publication number: 20200140935Abstract: The present invention provides assays and assay systems for use in spatially encoded biological assays. The invention provides an assay system comprising an assay capable of high levels of multiplexing where reagents are provided to a biological sample in defined spatial patterns; instrumentation capable of controlled delivery of reagents according to the spatial patterns; and a decoding scheme providing a readout that is digital in nature.Type: ApplicationFiled: January 3, 2020Publication date: May 7, 2020Applicant: Prognosys Biosciences, Inc.Inventor: Mark S. CHEE
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Publication number: 20200140934Abstract: The present invention provides assays and assay systems for use in spatially encoded biological assays. The invention provides an assay system comprising an assay capable of high levels of multiplexing where reagents are provided to a biological sample in defined spatial patterns; instrumentation capable of controlled delivery of reagents according to the spatial patterns; and a decoding scheme providing a readout that is digital in nature.Type: ApplicationFiled: January 3, 2020Publication date: May 7, 2020Applicant: Prognosys Biosciences, Inc.Inventor: Mark S. CHEE
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Patent number: 10619196Abstract: The present invention provides assays and assay systems for use in spatially encoded biological assays. The invention provides an assay system comprising an assay capable of high levels of multiplexing where reagents are provided to a biological sample in defined spatial patterns; instrumentation capable of controlled delivery of reagents according to the spatial patterns; and a decoding scheme providing a readout that is digital in nature.Type: GrantFiled: January 3, 2020Date of Patent: April 14, 2020Assignee: Prognosys Biosciences, Inc.Inventor: Mark S. Chee
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Publication number: 20200109443Abstract: The present disclosure provides methods and assay systems for use in spatially encoded biological assays, including assays to determine a spatial pattern of abundance, expression, and/or activity of one or more biological targets across multiple sites in a sample. In particular, the biological targets comprise proteins, and the methods and assay systems do not depend on imaging techniques for the spatial information of the targets. The present disclosure provides methods and assay systems capable of high levels of multiplexing where reagents are provided to a biological sample in order to address tag the sites to which reagents are delivered; instrumentation capable of controlled delivery of reagents; and a decoding scheme providing a readout that is digital in nature.Type: ApplicationFiled: October 8, 2019Publication date: April 9, 2020Applicant: Prognosys Biosciences, Inc.Inventors: Mark S. CHEE, David A. ROUTENBERG
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Patent number: 10612079Abstract: The present invention provides assays and assay systems for use in spatially encoded biological assays. The invention provides an assay system comprising an assay capable of high levels of multiplexing where reagents are provided to a biological sample in defined spatial patterns; instrumentation capable of controlled delivery of reagents according to the spatial patterns; and a decoding scheme providing a readout that is digital in nature.Type: GrantFiled: October 31, 2019Date of Patent: April 7, 2020Assignee: Prognosys Biosciences, Inc.Inventor: Mark S. Chee
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Publication number: 20200063195Abstract: The present invention provides assays and assay systems for use in spatially encoded biological assays. The invention provides an assay system comprising an assay capable of high levels of multiplexing where reagents are provided to a biological sample in defined spatial patterns; instrumentation capable of controlled delivery of reagents according to the spatial patterns; and a decoding scheme providing a readout that is digital in nature.Type: ApplicationFiled: October 30, 2019Publication date: February 27, 2020Applicant: Prognosys Biosciences, Inc.Inventor: Mark S. CHEE
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Publication number: 20200063196Abstract: The present invention provides assays and assay systems for use in spatially encoded biological assays. The invention provides an assay system comprising an assay capable of high levels of multiplexing where reagents are provided to a biological sample in defined spatial patterns; instrumentation capable of controlled delivery of reagents according to the spatial patterns; and a decoding scheme providing a readout that is digital in nature.Type: ApplicationFiled: October 31, 2019Publication date: February 27, 2020Applicant: Prognosys Biosciences, Inc.Inventor: Mark S. CHEE
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Publication number: 20200048702Abstract: The present invention provides oligonucleotide constructs, sets of such oligonucleotide constructs, and methods of using such oligonucleotide constructs to provide validated sequences or sets of validated sequences corresponding to desired ROIs. Such validated ROIs and constructs containing these have a wide variety of uses, including in synthetic biology, quantitative nucleic acid analysis, polymorphism and/or mutation screening, and the like.Type: ApplicationFiled: October 21, 2019Publication date: February 13, 2020Applicant: Prognosys Biosciences, Inc.Inventor: Mark S. CHEE
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Publication number: 20200048703Abstract: The present invention provides oligonucleotide constructs, sets of such oligonucleotide constructs, and methods of using such oligonucleotide constructs to provide validated sequences or sets of validated sequences corresponding to desired ROIs. Such validated ROIs and constructs containing these have a wide variety of uses, including in synthetic biology, quantitative nucleic acid analysis, polymorphism and/or mutation screening, and the like.Type: ApplicationFiled: October 21, 2019Publication date: February 13, 2020Applicant: Prognosys Biosciences, Inc.Inventor: Mark S. CHEE
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Publication number: 20200048701Abstract: The present invention provides oligonucleotide constructs, sets of such oligonucleotide constructs, and methods of using such oligonucleotide constructs to provide validated sequences or sets of validated sequences corresponding to desired ROIs. Such validated ROIs and constructs containing these have a wide variety of uses, including in synthetic biology, quantitative nucleic acid analysis, polymorphism and/or mutation screening, and the like.Type: ApplicationFiled: October 21, 2019Publication date: February 13, 2020Applicant: Prognosys Biosciences, Inc.Inventor: Mark S. CHEE
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Publication number: 20190383813Abstract: Provided herein are compositions and methods for detecting the binding of a peptide to an MHC molecule, and the binding of a peptide:MHC complex to a TCR. In preferred embodiments, the compositions and methods are in a highly-multiplexed way. The compositions and methods disclosed herein can be used to provide direct information on which peptides are bound to an MHC molecule. Also provided is a method for simultaneously detecting a large number of peptides for binding to an MHC molecule and/or a T cell. A method for detecting competitive binding of a large number of peptides to an MHC molecule and/or a T cell is also disclosed. Also provided herein is a method for simultaneously detecting a large number of specific TCRs. The compositions and methods of the present invention are useful for vaccine design, research and monitoring of autoimmune and infectious disease, immunogenicity testing of therapeutics, and tissue typing.Type: ApplicationFiled: May 21, 2019Publication date: December 19, 2019Applicant: Prognosys Biosciences, Inc.Inventors: John Andrew ALTIN, Mark S. CHEE
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Patent number: 10501793Abstract: The present invention provides oligonucleotide constructs, sets of such oligonucleotide constructs, and methods of using such oligonucleotide constructs to provide validated sequences or sets of validated sequences corresponding to desired ROIs. Such validated ROIs and constructs containing these have a wide variety of uses, including in synthetic biology, quantitative nucleic acid analysis, polymorphism and/or mutation screening, and the like.Type: GrantFiled: November 11, 2016Date of Patent: December 10, 2019Assignee: PROGNOSYS BIOSCIENCES, INC.Inventor: Mark S. Chee
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Patent number: 10494667Abstract: The present invention provides assays and assay systems for use in spatially encoded biological assays. The invention provides an assay system comprising an assay capable of high levels of multiplexing where reagents are provided to a biological sample in defined spatial patterns; instrumentation capable of controlled delivery of reagents according to the spatial patterns; and a decoding scheme providing a readout that is digital in nature.Type: GrantFiled: June 11, 2019Date of Patent: December 3, 2019Assignee: Prognosys Biosciences, Inc.Inventor: Mark S. Chee
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Patent number: 10480022Abstract: The present invention provides assays and assay systems for use in spatially encoded biological assays. The invention provides an assay system comprising an assay capable of high levels of multiplexing where reagents are provided to a biological sample in defined spatial patterns; instrumentation capable of controlled delivery of reagents according to the spatial patterns; and a decoding scheme providing a readout that is digital in nature.Type: GrantFiled: February 14, 2019Date of Patent: November 19, 2019Assignee: Prognosys Biosciences, Inc.Inventor: Mark S. Chee
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Patent number: 10472669Abstract: The present invention provides assays and assay systems for use in spatially encoded biological assays. The invention provides an assay system comprising an assay capable of high levels of multiplexing where reagents are provided to a biological sample in defined spatial patterns; instrumentation capable of controlled delivery of reagents according to the spatial patterns; and a decoding scheme providing a readout that is digital in nature.Type: GrantFiled: May 2, 2019Date of Patent: November 12, 2019Assignee: Prognosys Biosciences, Inc.Inventor: Mark S. Chee
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Publication number: 20190177778Abstract: The present invention provides assays and assay systems for use in spatially encoded biological assays. The invention provides an assay system comprising an assay capable of high levels of multiplexing where reagents are provided to a biological sample in defined spatial patterns; instrumentation capable of controlled delivery of reagents according to the spatial patterns; and a decoding scheme providing a readout that is digital in nature.Type: ApplicationFiled: February 14, 2019Publication date: June 13, 2019Applicant: Prognosys Biosciences, Inc.Inventor: Mark S. CHEE
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Publication number: 20190177777Abstract: The present invention provides assays and assay systems for use in spatially encoded biological assays. The invention provides an assay system comprising an assay capable of high levels of multiplexing where reagents are provided to a biological sample in defined spatial patterns; instrumentation capable of controlled delivery of reagents according to the spatial patterns; and a decoding scheme providing a readout that is digital in nature.Type: ApplicationFiled: February 14, 2019Publication date: June 13, 2019Applicant: Prognosys Biosciences, Inc.Inventor: Mark S. CHEE
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Patent number: 10308982Abstract: The present invention provides assays and assay systems for use in spatially encoded biological assays. The invention provides an assay system comprising an assay capable of high levels of multiplexing where reagents are provided to a biological sample in defined spatial patterns; instrumentation capable of controlled delivery of reagents according to the spatial patterns; and a decoding scheme providing a readout that is digital in nature.Type: GrantFiled: June 20, 2016Date of Patent: June 4, 2019Assignee: Prognosys Biosciences, Inc.Inventor: Mark S. Chee
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Patent number: 10288608Abstract: The present disclosure provides polynucleotide conjugates, methods, and assay systems for use in detecting the presence, absence, and/or amount of an analyte in a sample. Various polynucleotide conjugates, conjugate pairs, sets, libraries, and assay systems comprising the same are disclosed. In particular, methods and assay systems for antibody detection and analysis are provided. For example, assays capable of high levels of multiplexing are used for antibody detection and analysis in a biological sample, e.g., Lyme disease patient samples. The presently disclosed polynucleotide conjugates, methods, and assay systems can be used to provide sensitive and reliable diagnosis, even at early stages of a disease or condition. Use for monitoring disease progression and prognosis is also disclosed.Type: GrantFiled: November 7, 2014Date of Patent: May 14, 2019Assignee: Prognosys Biosciences, Inc.Inventors: Igor A. Kozlov, John Andrew Altin, Petr Capek, Mark S. Chee
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Patent number: 10266883Abstract: The present invention provides oligonucleotide constructs, sets of such oligonucleotide constructs, and methods of using such oligonucleotide constructs to provide validated sequences or sets of validated sequences corresponding to desired ROIs. Such validated ROIs and constructs containing these have a wide variety of uses, including in synthetic biology, quantitative nucleic acid analysis, polymorphism and/or mutation screening, and the like.Type: GrantFiled: November 11, 2016Date of Patent: April 23, 2019Assignee: Prognosys Biosciences, Inc.Inventor: Mark S. Chee