Abstract: Disclosed herein are methods and compositions for targeted cleavage of a genomic sequence, targeted alteration of a genomic sequence, and targeted recombination between a genomic region and an exogenous polynucleotide homologous to the genomic region. The compositions include fusion proteins comprising a cleavage domain (or cleavage half-domain) and an engineered zinc finger domain and polynucleotides encoding same. Methods for targeted cleavage include introduction of such fusion proteins, or polynucleotides encoding same, into a cell. Methods for targeted recombination additionally include introduction of an exogenous polynucleotide homologous to a genomic region into cells comprising the disclosed fusion proteins.
Type:
Grant
Filed:
August 6, 2004
Date of Patent:
February 15, 2011
Assignee:
Sangamo Biosciences, Inc.
Inventors:
Fyodor Urnov, Michael C. Holmes, Jeffrey C. Miller, Carl O. Pabo
Abstract: Disclosed herein are methods and compositions for targeted integration of an exogenous sequence into a predetermined target site in a plant genome.
Type:
Application
Filed:
August 9, 2007
Publication date:
October 7, 2010
Applicants:
Dow AgroSciences LLC, Sangamo BioSciences, Inc.
Inventors:
Qihua Cai, Jeffrey C. Miller, William M. Ainley, Robbi J. Garrison, Joseph F. Petolino, Beth C. Rubin-Wilson, Lisa L. Schulenberg, Andrew F. Worden
Abstract: Disclosed herein are methods and compositions for treatment of conditions requiring inhibition of angiogenesis. Such conditions include those characterized by neovascularization, such as retinopathies, macular degeneration and various malignancies.
Abstract: The invention provides criteria and methods for selecting optimum subsequence(s) from a target gene for targeting by a zinc finger protein. Some of the methods of target site selection seek to identify one or more target segments having a DNA motif containing one or more so-called D-able subsites having the sequence 5?NNGK3?. Other methods of the invention are directed to selection of target segments within target genes using a correspondence regime between different triplets of three bases and the three possible positions of a triplet within a nine-base site. In another aspect, the invention provides methods of designing zinc finger proteins that bind to a preselected target site. These methods can be used following the preselection of target sites according to the procedures and criteria described above. The methods of design use a database containing information about previously characterized zinc finger proteins.
Type:
Grant
Filed:
February 12, 2007
Date of Patent:
August 31, 2010
Assignee:
Sangamo Biosciences, Inc.
Inventors:
Stephen P. Eisenberg, Casey C. Case, George N. Cox, III, Andrew Jamieson, Edward J. Rebar
Abstract: Methods and compositions for targeted modification of chromatin structure, within a region of interest in cellular chromatin, are provided. Such methods and compositions are useful for facilitating processes such as, for example, transcription and recombination, that require access of exogenous molecules to chromosomal DNA sequences.
Type:
Grant
Filed:
July 10, 2008
Date of Patent:
August 31, 2010
Assignee:
Sangamo Biosciences, Inc.
Inventors:
Alan P. Wolffe, Elizabeth J. Wolffe, legal representative, Trevor Collingwood, Philip D. Gregory, Andrew Snowden, Fyodor Urnov
Abstract: Disclosed herein are methods and compositions for treatment of conditions requiring inhibition of angiogenesis. Such conditions include those characterized by neovascularization, such as retinopathies, macular degeneration and various malignancies.
Type:
Grant
Filed:
December 6, 2007
Date of Patent:
June 8, 2010
Assignee:
Sangamo Biosciences, Inc.
Inventors:
H. Steve Zhang, Philip D. Gregory, Edward J. Rebar
Abstract: Disclosed herein are modified plant zinc finger proteins; compositions comprising modified plant zinc finger proteins and methods of making and using modified plant zinc finger proteins. The modified plant zinc finger proteins, in contrast to naturally-occurring plant zinc finger proteins, have a binding specificity that is determined by tandem arrays of modular zinc finger binding unit.
Abstract: Provided herein are a variety of methods and compositions for regulating angiogenesis, such methods and compositions being useful in a variety of applications where modulation of vascular formation is useful, including, but not limited to, treatments for ischemia and wound healing. Certain of the methods and compositions accomplish this by using various zinc finger proteins that bind to particular target sites in one or more VEGF genes. Nucleic acids encoding the zinc finger proteins are also disclosed. Methods for modulating the expression of one or more VEGF genes with the zinc finger proteins and nucleic acids are also disclosed. Such methods can also be utilized in a variety of therapeutic applications that involve the regulation of endothelial cell growth. Pharmaceutical compositions including the zinc finger proteins or nucleic acids encoding them are also provided.
Type:
Application
Filed:
September 3, 2009
Publication date:
April 15, 2010
Applicant:
Sangamo BioSciences, Inc.
Inventors:
Edward Rebar, Andrew Jamieson, Qiang Liu, Pei-Qi Liu, Alan Wolffe, Stephen P. Eisenberg, Eric Jarvis
Abstract: Disclosed is a method for the administration of zinc finger proteins (ZFPs) or nucleic acids that encode such ZFPs for treating peripheral arterial disease, particularly by the repeated administration at regular intervals if such ZFPs or nucleic acids that encode such ZFPs.
Type:
Application
Filed:
February 9, 2007
Publication date:
December 10, 2009
Applicant:
Sangamo BioScience, Inc.
Inventors:
Patrice Tremble, Joe Rokovich, Brian Annex
Abstract: Provided herein are a variety of methods and compositions for regulating angiogenesis, such methods and compositions being useful in a variety of applications where modulation of vascular formation is useful, including, but not limited to, treatments for ischemia and wound healing. Certain of the methods and compositions accomplish this by using various zinc finger proteins that bind to particular target sites in one or more VEGF genes. Nucleic acids encoding the zinc finger proteins are also disclosed. Methods for modulating the expression of one or more VEGF genes with the zinc finger proteins and nucleic acids are also disclosed. Such methods can also be utilized in a variety of therapeutic applications that involve the regulation of endothelial cell growth. Pharmaceutical compositions including the zinc finger proteins or nucleic acids encoding them are also provided.
Type:
Grant
Filed:
April 26, 2005
Date of Patent:
October 20, 2009
Assignee:
Sangamo Biosciences, Inc.
Inventors:
Edward Rebar, Andrew Jamieson, Qiang Liu, Pei-Qi Liu, Alan Wolffe, Stephen P. Eisenberg, Eric Jarvis
Abstract: The specificity of binding of a zinc finger to a triplet or quadruplet nucleotide target subsite depends upon the location of the zinc finger in a multifinger protein and, hence, upon the location of its target subsite within a larger target sequence. The present disclosure provides zinc finger amino acid sequences for recognition of triplet target subsites having the nucleotide G in the 5?-most position of the subsite, that have been optimized with respect to the location of the subsite within the target site. Accordingly, the disclosure provides finger position-specific amino acid sequences for the recognition of GNN target subsites. This allows the construction of multi-finger zinc finger proteins with improved affinity and specificity for their target sequences, as well as enhanced biological activity.
Type:
Grant
Filed:
August 11, 2005
Date of Patent:
September 8, 2009
Assignee:
Sangamo Biosciences, Inc.
Inventors:
Qiang Liu, Edward Rebar, Andrew C. Jamieson
Abstract: A variety of zinc finger proteins (ZFPs) and methods utilizing such proteins are provided for use in treating chronic pain. ZFPs that bind to a target site in genes that are aberrantly expressed in subjects having chronic pain are described. In addition, ZFPs that bind to a target site in genes expressed at normal levels in subjects experiencing chronic pain, modulation of whose expression results in decreased pain perception, are also provided. For example, genes that are over-expressed in the dorsal root ganglia (DRG) of pain patients (e.g., Nav1.8) can be repressed.
Type:
Application
Filed:
February 6, 2009
Publication date:
August 27, 2009
Applicant:
SANGAMO BIOSCIENCES, INC.
Inventors:
SIYUAN TAN, STEVE H. ZHANG, JEFFREY C. MILLER, PHILIP D. GREGORY
Abstract: Methods and compositions are provided for modulating cardiac contractility by regulating transcription of the phsopholamban gene using engineered zinc finger proteins.
Abstract: A variety of zinc finger proteins (ZFPs) and methods utilizing such proteins are provided for use in treating neuropathic pain. ZFPs that bind to a target site in genes that are aberrantly expressed in subjects having neuropathic pain are described. In addition, ZFPs that bind to a target site in genes expressed at normal levels in subjects experiencing neuropathic pain, modulation of whose expression results in decreased pain perception, are also provided. For example, genes that are over-expressed in the dorsal root ganglia (DRG) of pain patients (e.g., VR1, TRKA and/or Nav1.8) can be repressed, whereas genes that are under-expressed in the same populations can be activated.
Abstract: The present invention relates to methods of using libraries of randomized zinc finger proteins to identify genes associated with selected phenotypes.
Type:
Grant
Filed:
January 6, 2003
Date of Patent:
February 17, 2009
Assignee:
Sangamo BioSciences, Inc.
Inventors:
Casey C. Case, Edward J. Rebar, Andrew Jamieson
Abstract: Disclosed herein are methods and compositions for treatment of conditions requiring inhibition of angiogenesis. Such conditions include those characterized by neovascularization, such as retinopathies, macular degeneration and various malignancies.
Abstract: The invention provides criteria and methods for selecting optimum subsequence(s) from a target gene for targeting by a zinc finger protein. Some of the methods of target site selection seek to identify one or more target segments having a DNA motif containing one or more so-called D-able subsites having the sequence 5?NNGK3?. Other methods of the invention are directed to selection of target segments within target genes using a correspondence regime between different triplets of three bases and the three possible positions of a triplet within a nine-base site. In another aspect, the invention provides methods of designing zinc finger proteins that bind to a preselected target site. These methods can be used following the preselection of target sites according to the procedures and criteria described above. The methods of design use a database containing information about previously characterized zinc finger proteins.
Type:
Application
Filed:
February 12, 2007
Publication date:
December 13, 2007
Applicant:
Sangamo BioSciences, Inc.
Inventors:
Stephen Eisenberg, Casey Case, George Cox lll, Andrew Jamieson, Edward Rebar
Abstract: Disclosed herein are modified plant zinc finger proteins; compositions comprising modified plant zinc finger proteins and methods of making and using modified plant zinc finger proteins. The modified plant zinc finger proteins, in contrast to naturally-occurring plant zinc finger proteins, have a binding specificity that is determined by tandem arrays of modular zinc finger binding units.