Patents Assigned to Sangamo Biosciences, Inc.
  • Methods and compositions for treating neuropathies
    Patent number: 8551945
    Abstract: Disclosed herein are methods and compositions for treating neuropathies by modulating endogenous NT-3 of GDNF gene expression.
    Type: Grant
    Filed: February 4, 2010
    Date of Patent: October 8, 2013
    Assignee: Sangamo BioSciences, Inc.
    Inventors: Carolyn Dent, Josee Laganiere, Xiangdong Meng, David Paschon, Siyuan Tan, Lei Zhang, Steve H. Zhang
  • METHODS AND COMPOSITIONS FOR TREATING HUNTINGTON'S DISEASE
    Publication number: 20130253040
    Abstract: Disclosed herein are methods and compositions for treating or preventing Huntington's Disease.
    Type: Application
    Filed: February 28, 2013
    Publication date: September 26, 2013
    Applicant: C/O SANGAMO BIOSCIENCES, INC.
    Inventors: Jeffrey C. Miller, Edward J. Rebar, H. Steve Zhang
  • TAREGTED INTEGRATION AND EXPRESSION OF EXOGENOUS NUCLEIC ACID SEQUENCES
    Publication number: 20130244332
    Abstract: Disclosed herein are methods and compositions for targeted integration of a exogenous sequence into a predetermined target site in a genome for use, for example, in protein expression and gene inactivation.
    Type: Application
    Filed: March 6, 2013
    Publication date: September 19, 2013
    Applicant: SANGAMO BIOSCIENCES, INC.
    Inventors: Philip D. Gregory, Michael C. Holmes, Edward J. Rebar, Fyodor Urnov
  • Methods and compositions for targeted cleavage and recombination
    Patent number: 8524500
    Abstract: Disclosed herein are methods and compositions for targeted cleavage of a genomic sequence, targeted alteration of a genomic sequence, and targeted recombination between a genomic region and an exogenous polynucleotide homologous to the genomic region. The compositions include fusion proteins comprising a cleavage domain (or cleavage half-domain) and an engineered zinc finger domain and polynucleotides encoding same. Methods for targeted cleavage include introduction of such fusion proteins, or polynucleotides encoding same, into a cell. Methods for targeted recombination additionally include introduction of an exogenous polynucleotide homologous to a genomic region into cells comprising the disclosed fusion proteins.
    Type: Grant
    Filed: July 16, 2010
    Date of Patent: September 3, 2013
    Assignee: Sangamo BioSciences, Inc.
    Inventors: Fyodor Urnov, Michael C. Holmes, Jeffrey C. Miller, Carl O. Pabo
  • Methods and compositions for gene inactivation
    Patent number: 8524221
    Abstract: Disclosed herein are methods and compositions for inactivating CCR-5 genes, using zinc finger nucleases (ZFNs) comprising a zinc finger protein and a cleavage domain or cleavage half-domain. Polynucleotides encoding ZFNs, vectors comprising polynucleotides encoding ZFNs, such as adenovirus (Ad) vectors, and cells comprising polynucleotides encoding ZFNs and/or cells comprising ZFNs are also provided.
    Type: Grant
    Filed: May 5, 2011
    Date of Patent: September 3, 2013
    Assignee: Sangamo BioSciences, Inc.
    Inventors: Dale Ando, Michael C. Holmes, Yann Jouvenot, Gary Ka Leong Lee
  • METHODS AND COMPOSTITIONS FOR GENE EDITING OF A PATHOGEN
    Publication number: 20130216579
    Abstract: Disclosed herein are methods and compositions for genome editing of the malarial parasite Plasmodium, and for the use of the edited Plasmodium in the development of vaccines and therapeutics.
    Type: Application
    Filed: January 23, 2013
    Publication date: August 22, 2013
    Applicants: The Trustees of Columbia University in the City of New York, Sangamo BioSciences, Inc.
    Inventors: Sangamo BioSciences, Inc., The Trustees of Columbia University in the City of New York
  • MODIFIED DNA-BINDING PROTEINS AND USES THEREOF
    Publication number: 20130196373
    Abstract: Disclosed herein are enhanced polypeptides, polynucleotides encoding these polypeptides, cells and organisms comprising novel DNA-binding domains, including TALE DNA-binding domains. Also disclosed are methods of using these novel DNA-binding domains for modulation of gene expression and/or genomic editing of endogenous cellular sequences.
    Type: Application
    Filed: November 16, 2012
    Publication date: August 1, 2013
    Applicant: SANGAMO BIOSCIENCES, INC.
    Inventor: Sangamo BioSciences, Inc.
  • ORGANISMS HOMOZYGOUS FOR TARGETED MODIFICATION
    Publication number: 20130198878
    Abstract: Disclosed herein are homozygously modified organisms and methods of making and using these organisms.
    Type: Application
    Filed: March 14, 2013
    Publication date: August 1, 2013
    Applicant: C/O SANGAMO BIOSCIENCES, INC.
    Inventor: C/O SANGAMO BIOSCIENCES, INC.
  • METHODS AND COMPOSITIONS FOR REGULATION OF TRANSGENE EXPRESSION
    Publication number: 20130177960
    Abstract: Nucleases and methods of using these nucleases for expressing a transgene from a safe harbor locus in a secretory tissue, and clones and animals derived therefrom.
    Type: Application
    Filed: September 21, 2012
    Publication date: July 11, 2013
    Applicant: Sangamo BioSciences, Inc.
    Inventor: Sangamo BioSciences, Inc.
  • METHODS AND COMPOSITIONS FOR REGULATION OF TRANSGENE EXPRESSION
    Publication number: 20130177983
    Abstract: Nucleases and methods of using these nucleases for expressing a transgene from a safe harbor locus in a secretory tissue, and clones and animals derived therefrom.
    Type: Application
    Filed: September 21, 2012
    Publication date: July 11, 2013
    Applicant: Sangamo BioScience, Inc.
    Inventor: Sangamo BioScience, Inc.
  • RAPID SCREENING OF BIOLOGICALLY ACTIVE NUCLEASES AND ISOLATION OF NUCLEASE-MODIFIED CELLS
    Publication number: 20130171657
    Abstract: Disclosed herein are methods and compositions for rapidly identifying active nucleases and cells having nuclease-mediated genomic modifications.
    Type: Application
    Filed: March 11, 2013
    Publication date: July 4, 2013
    Applicant: SANGAMO BIOSCIENCES, INC.
    Inventor: Sangamo BioSciences, Inc.
  • METHODS AND COMPOSITIONS FOR REGULATING HIV INFECTION
    Publication number: 20130171732
    Abstract: Methods and compositions for regulating HIV infection and/or replication in which an anti-HIV transgene is integrated into the genome of a cell using a nuclease.
    Type: Application
    Filed: October 4, 2012
    Publication date: July 4, 2013
    Applicant: SANGAMO BIOSCIENCES, INC.
    Inventor: Sangamo BioSciences, Inc.
  • Nucleic acid encoding a zinc finger that recognizes the sodium channel Nav 1.8 (PN3) gene
    Patent number: 8466267
    Abstract: A variety of zinc finger proteins (ZFPs) and methods utilizing such proteins are provided for use in treating neuropathic pain. ZFPs that bind to a target site in genes that are aberrantly expressed in subjects having neuropathic pain are described. In addition, ZFPs that bind to a target site in genes expressed at normal levels in subjects experiencing neuropathic pain, modulation of whose expression results in decreased pain perception, are also provided. For example, genes that are over-expressed in the dorsal root ganglia (DRG) of pain patients (e.g., VR1, TRKA and/or Nav1.8) can be repressed, whereas genes that are under-expressed in the same populations can be activated.
    Type: Grant
    Filed: July 5, 2007
    Date of Patent: June 18, 2013
    Assignee: Sangamo Biosciences, Inc.
    Inventor: Trevor Collingwood
  • ENGINEERED ZINC FINGER PROTEINS TARGETING 5-ENOLPYRUVYL SHIKIMATE-3-PHOSPHATE SYNTHASE GENES
    Publication number: 20130145503
    Abstract: The present disclosure relates to engineered zinc finger proteins that target 5-enolpyruvyl shikimate-3-phosphate synthase (EPSPS) genes in plants and methods of using such zinc finger proteins in modulating gene expression, gene inactivation, and targeted gene modification. In particular, the disclosure pertains to zinc finger nucleases for targeted cleavage and alteration of EPSPS genes.
    Type: Application
    Filed: February 13, 2013
    Publication date: June 6, 2013
    Applicants: C/O SANGAMO BIOSCIENCES, INC., C/O DOW AGROSCIENCES LLC
    Inventors: c/o Dow AgroSciences LLC, c/o Sangamo BioSciences, Inc.
  • METHODS AND COMPOSITIONS FOR MODIFICATION OF THE HPRT LOCUS
    Publication number: 20130137104
    Abstract: Nucleases and methods of using these nucleases for modification of an HPRT locus and for increasing the frequency of gene modification at a targeted locus and clones and for generating animals.
    Type: Application
    Filed: October 25, 2012
    Publication date: May 30, 2013
    Applicants: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA, SANGAMO BIOSCIENCES, INC.
    Inventors: SANGAMO BIOSCIENCES, INC., THE REGENTS OF THE UNIVERSITY OF CALIF
  • POSITION DEPENDENT RECOGNITION OF GNN NUCLEOTIDE TRIPLETS BY ZINC FINGERS
    Publication number: 20130123484
    Abstract: The specificity of binding of a zinc finger to a triplet or quadruplet nucleotide target subsite depends upon the location of the zinc finger in a multifinger protein and, hence, upon the location of its target subsite within a larger target sequence. The present disclosure provides zinc finger amino acid sequences for recognition of triplet target subsites having the nucleotide G in the 5?-most position of the subsite, that have been optimized with respect to the location of the subsite within the target site. Accordingly, the disclosure provides finger position-specific amino acid sequences for the recognition of GNN target subsites. This allows the construction of multi-finger zinc finger proteins with improved affinity and specificity for their target sequences, as well as enhanced biological activity.
    Type: Application
    Filed: January 16, 2013
    Publication date: May 16, 2013
    Applicant: SANGAMO BIOSCIENCES, INC.
    Inventor: Sangamo Biosciences, Inc.
  • METHODS AND COMPOSITIONS FOR MODIFICATION OF THE HPRT LOCUS
    Publication number: 20130122591
    Abstract: Nucleases and methods of using these nucleases for modification of an HPRT locus and for increasing the frequency of gene modification at a targeted locus and clones and for generating animals.
    Type: Application
    Filed: October 25, 2012
    Publication date: May 16, 2013
    Applicants: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA, SANGAMO BIOSCIENCES, INC.
    Inventors: Sangamo BioSciences, Inc., The Regents of the University of California
  • Targeted deletion of cellular DNA sequences
    Patent number: 8409861
    Abstract: Disclosed herein are methods and compositions for targeted deletion of double-stranded DNA. The compositions include fusion proteins comprising a cleavage domain (or cleavage half-domain) and an engineered zinc finger domain, and polynucleotides encoding same. Methods for targeted deletion include introduction of such fusion proteins, or polynucleotides encoding same, into a cell such that two targeted cleavage events occur. Subsequent cellular repair mechanisms result in deletion of sequences between the two cleavage sites.
    Type: Grant
    Filed: December 15, 2005
    Date of Patent: April 2, 2013
    Assignee: Sangamo BioSciences, Inc.
    Inventors: Dmitry Guschin, Michael C. Holmes
  • Position dependent recognition of GNN nucleotide triplets by zinc fingers
    Patent number: 8383766
    Abstract: The specificity of binding of a zinc finger to a triplet or quadruplet nucleotide target subsite depends upon the location of the zinc finger in a multifinger protein and, hence, upon the location of its target subsite within a larger target sequence. The present disclosure provides zinc finger amino acid sequences for recognition of triplet target subsites having the nucleotide G in the 5?-most position of the subsite, that have been optimized with respect to the location of the subsite within the target site. Accordingly, the disclosure provides finger position-specific amino acid sequences for the recognition of GNN target subsites. This allows the construction of multi-finger zinc finger proteins with improved affinity and specificity for their target sequences, as well as enhanced biological activity.
    Type: Grant
    Filed: August 15, 2007
    Date of Patent: February 26, 2013
    Assignee: Sangamo BioSciences, Inc.
    Inventors: Qiang Liu, Edward Rebar, Andrew C. Jamieson
  • Methods for targeted cleavage and recombination of CCR5
    Patent number: 8349810
    Abstract: Disclosed herein are methods and compositions for targeted cleavage of a genomic sequence, targeted alteration of a genomic sequence, and targeted recombination between a genomic region and an exogenous polynucleotide homologous to the genomic region. The compositions include fusion proteins comprising a cleavage domain (or cleavage half-domain) and an engineered zinc finger domain, as well as polynucleotides encoding same. Fusion proteins comprising cleavage half-domains are used in pairs to reconstitute a functional cleavage domain. In these fusion proteins, the zinc finger domain can be N-terminal to the cleavage half-domain, or the cleavage half-domain can be N-terminal to the zinc finger domain.
    Type: Grant
    Filed: June 22, 2011
    Date of Patent: January 8, 2013
    Assignee: Sangamo BioSciences, Inc.
    Inventors: Jeffrey C. Miller, Lei Zhang